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- PDB-6c2f: MBD2 in complex with methylated DNA -

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Basic information

Entry
Database: PDB / ID: 6c2f
TitleMBD2 in complex with methylated DNA
Components
  • (12-mer DNA) x 2
  • Methyl-CpG-binding domain protein 2
KeywordsDNA BINDING PROTEIN/DNA / methylated dna / dna binding / Structural Genomics / Structural Genomics Consortium / SGC / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


satellite DNA binding / NuRD complex / DNA methylation-dependent heterochromatin formation / methyl-CpG binding / C2H2 zinc finger domain binding / RNA Polymerase I Promoter Opening / NoRC negatively regulates rRNA expression / molecular adaptor activity / chromatin remodeling / protein domain specific binding ...satellite DNA binding / NuRD complex / DNA methylation-dependent heterochromatin formation / methyl-CpG binding / C2H2 zinc finger domain binding / RNA Polymerase I Promoter Opening / NoRC negatively regulates rRNA expression / molecular adaptor activity / chromatin remodeling / protein domain specific binding / negative regulation of DNA-templated transcription / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / nucleus / cytosol
Similarity search - Function
Methyl-CpG binding protein 2/3, C-terminal domain / Methyl-CpG-binding domain protein 2/3, p55-binding region / C-terminal domain of methyl-CpG binding protein 2 and 3 / p55-binding region of Methyl-CpG-binding domain proteins MBD / Methyl-cpg-binding Protein 2; Chain A / Methyl-cpg-binding Protein 2; Chain A / Methyl-CpG binding domain / Methyl-CpG DNA binding / Methyl-CpG binding domain / Methyl-CpG-binding domain (MBD) profile. ...Methyl-CpG binding protein 2/3, C-terminal domain / Methyl-CpG-binding domain protein 2/3, p55-binding region / C-terminal domain of methyl-CpG binding protein 2 and 3 / p55-binding region of Methyl-CpG-binding domain proteins MBD / Methyl-cpg-binding Protein 2; Chain A / Methyl-cpg-binding Protein 2; Chain A / Methyl-CpG binding domain / Methyl-CpG DNA binding / Methyl-CpG binding domain / Methyl-CpG-binding domain (MBD) profile. / DNA-binding domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / Methyl-CpG-binding domain protein 2
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.65 Å
AuthorsLiu, K. / Xu, C. / Tempel, W. / Arrowsmith, C.H. / Bountra, C. / Edwards, A.M. / Min, J. / Structural Genomics Consortium / Structural Genomics Consortium (SGC)
CitationJournal: to be published
Title: MBD2 in complex with methylated DNA
Authors: Liu, K. / Xu, C. / Tempel, W. / Arrowsmith, C.H. / Bountra, C. / Edwards, A.M. / Min, J. / Structural Genomics Consortium
History
DepositionJan 8, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 14, 2018Provider: repository / Type: Initial release
Revision 1.1Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Methyl-CpG-binding domain protein 2
B: 12-mer DNA
C: 12-mer DNA
D: Methyl-CpG-binding domain protein 2
E: 12-mer DNA
F: 12-mer DNA
G: Methyl-CpG-binding domain protein 2
H: 12-mer DNA
I: 12-mer DNA
J: Methyl-CpG-binding domain protein 2
K: 12-mer DNA
L: 12-mer DNA
M: Methyl-CpG-binding domain protein 2
N: 12-mer DNA
O: 12-mer DNA
P: Methyl-CpG-binding domain protein 2
Q: 12-mer DNA
R: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)96,79229
Polymers96,79218
Non-polymers011
Water0
1
A: Methyl-CpG-binding domain protein 2
B: 12-mer DNA
C: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1325
Polymers16,1323
Non-polymers02
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3140 Å2
ΔGint-14 kcal/mol
Surface area7560 Å2
MethodPISA
2
D: Methyl-CpG-binding domain protein 2
E: 12-mer DNA
F: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1325
Polymers16,1323
Non-polymers02
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3080 Å2
ΔGint-14 kcal/mol
Surface area7500 Å2
MethodPISA
3
G: Methyl-CpG-binding domain protein 2
H: 12-mer DNA
I: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1325
Polymers16,1323
Non-polymers02
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3130 Å2
ΔGint-14 kcal/mol
Surface area7540 Å2
MethodPISA
4
J: Methyl-CpG-binding domain protein 2
K: 12-mer DNA
L: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1325
Polymers16,1323
Non-polymers02
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3020 Å2
ΔGint-14 kcal/mol
Surface area7460 Å2
MethodPISA
5
M: Methyl-CpG-binding domain protein 2
N: 12-mer DNA
O: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1325
Polymers16,1323
Non-polymers02
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3080 Å2
ΔGint-12 kcal/mol
Surface area7450 Å2
MethodPISA
6
P: Methyl-CpG-binding domain protein 2
Q: 12-mer DNA
R: 12-mer DNA


Theoretical massNumber of molelcules
Total (without water)16,1324
Polymers16,1323
Non-polymers01
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3020 Å2
ΔGint-13 kcal/mol
Surface area7460 Å2
MethodPISA
Unit cell
Length a, b, c (Å)40.510, 40.600, 202.380
Angle α, β, γ (deg.)90.070, 93.110, 119.750
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 148 through 160 or (resid 161...
21(chain D and (resid 148 through 150 or (resid 151...
31(chain G and (resid 148 through 160 or (resid 161...
41(chain J and (resid 148 through 159 or (resid 160...
51(chain M and (resid 148 through 149 or (resid 150...
61(chain P and (resid 148 through 150 or (resid 151...
12chain C
22chain F
32chain I
42chain L
52chain O
62chain R
13chain B
23chain E
33chain H
43chain K
53chain N
63chain Q

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LYSLYSLYSLYS(chain A and (resid 148 through 160 or (resid 161...AA148 - 1607 - 19
121LYSLYSLYSLYS(chain A and (resid 148 through 160 or (resid 161...AA16120
131GLYGLYPROPRO(chain A and (resid 148 through 160 or (resid 161...AA147 - 2156 - 74
141GLYGLYPROPRO(chain A and (resid 148 through 160 or (resid 161...AA147 - 2156 - 74
151GLYGLYPROPRO(chain A and (resid 148 through 160 or (resid 161...AA147 - 2156 - 74
161GLYGLYPROPRO(chain A and (resid 148 through 160 or (resid 161...AA147 - 2156 - 74
211LYSLYSMETMET(chain D and (resid 148 through 150 or (resid 151...DD148 - 1507 - 9
221ASPASPASPASP(chain D and (resid 148 through 150 or (resid 151...DD15110
231LYSLYSPROPRO(chain D and (resid 148 through 150 or (resid 151...DD148 - 2157 - 74
241LYSLYSPROPRO(chain D and (resid 148 through 150 or (resid 151...DD148 - 2157 - 74
251LYSLYSPROPRO(chain D and (resid 148 through 150 or (resid 151...DD148 - 2157 - 74
261LYSLYSPROPRO(chain D and (resid 148 through 150 or (resid 151...DD148 - 2157 - 74
311LYSLYSLYSLYS(chain G and (resid 148 through 160 or (resid 161...GG148 - 1607 - 19
321LYSLYSLYSLYS(chain G and (resid 148 through 160 or (resid 161...GG16120
331LYSLYSPROPRO(chain G and (resid 148 through 160 or (resid 161...GG148 - 2157 - 74
341LYSLYSPROPRO(chain G and (resid 148 through 160 or (resid 161...GG148 - 2157 - 74
351LYSLYSPROPRO(chain G and (resid 148 through 160 or (resid 161...GG148 - 2157 - 74
361LYSLYSPROPRO(chain G and (resid 148 through 160 or (resid 161...GG148 - 2157 - 74
411LYSLYSTRPTRP(chain J and (resid 148 through 159 or (resid 160...JJ148 - 1597 - 18
421LYSLYSLYSLYS(chain J and (resid 148 through 159 or (resid 160...JJ160 - 16119 - 20
431LYSLYSMETMET(chain J and (resid 148 through 159 or (resid 160...JJ148 - 2147 - 73
441LYSLYSMETMET(chain J and (resid 148 through 159 or (resid 160...JJ148 - 2147 - 73
451LYSLYSMETMET(chain J and (resid 148 through 159 or (resid 160...JJ148 - 2147 - 73
461LYSLYSMETMET(chain J and (resid 148 through 159 or (resid 160...JJ148 - 2147 - 73
511LYSLYSARGARG(chain M and (resid 148 through 149 or (resid 150...MM148 - 1497 - 8
521METMETASPASP(chain M and (resid 148 through 149 or (resid 150...MM150 - 1519 - 10
531LYSLYSPROPRO(chain M and (resid 148 through 149 or (resid 150...MM148 - 2157 - 74
541LYSLYSPROPRO(chain M and (resid 148 through 149 or (resid 150...MM148 - 2157 - 74
551LYSLYSPROPRO(chain M and (resid 148 through 149 or (resid 150...MM148 - 2157 - 74
561LYSLYSPROPRO(chain M and (resid 148 through 149 or (resid 150...MM148 - 2157 - 74
611LYSLYSMETMET(chain P and (resid 148 through 150 or (resid 151...PP148 - 1507 - 9
621ASPASPASPASP(chain P and (resid 148 through 150 or (resid 151...PP15110
631LYSLYSPROPRO(chain P and (resid 148 through 150 or (resid 151...PP148 - 2157 - 74
641LYSLYSPROPRO(chain P and (resid 148 through 150 or (resid 151...PP148 - 2157 - 74
651LYSLYSPROPRO(chain P and (resid 148 through 150 or (resid 151...PP148 - 2157 - 74
661LYSLYSPROPRO(chain P and (resid 148 through 150 or (resid 151...PP148 - 2157 - 74
112DGDGDCDCchain CCC1 - 121 - 12
212DGDGDCDCchain FFF1 - 121 - 12
312DGDGDCDCchain III1 - 121 - 12
412DGDGDCDCchain LLL1 - 121 - 12
512DGDGDCDCchain OOO1 - 121 - 12
612DGDGDCDCchain RRR1 - 121 - 12
113DGDGDCDCchain BBB1 - 121 - 12
213DGDGDCDCchain EEE1 - 121 - 12
313DGDGDCDCchain HHH1 - 121 - 12
413DGDGDCDCchain KKK1 - 121 - 12
513DGDGDCDCchain NNN1 - 121 - 12
613DGDGDCDCchain QQQ1 - 121 - 12

NCS ensembles :
ID
1
2
3

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Components

#1: Protein
Methyl-CpG-binding domain protein 2 / / Demethylase / DMTase / Methyl-CpG-binding protein MBD2


Mass: 8791.171 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MBD2 / Plasmid: pET28-MHL / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-V2R-pRARE2 / References: UniProt: Q9UBB5
#2: DNA chain
12-mer DNA


Mass: 3726.457 Da / Num. of mol.: 6 / Source method: obtained synthetically / Details: synthetic / Source: (synth.) synthetic construct (others)
#3: DNA chain
12-mer DNA


Mass: 3614.354 Da / Num. of mol.: 6 / Source method: obtained synthetically / Details: synthetic / Source: (synth.) synthetic construct (others)
#4: Chemical
ChemComp-UNX / UNKNOWN ATOM OR ION


Num. of mol.: 11 / Source method: obtained synthetically

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.2 Å3/Da / Density % sol: 61.3 %
Crystal growTemperature: 291 K / Method: vapor diffusion / Details: 20% PEG-3350, 0.2 M potassium acetate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU FR-E SUPERBRIGHT / Wavelength: 1.5418 Å
DetectorType: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jul 3, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2.55→35.1 Å / Num. obs: 36264 / % possible obs: 99.7 % / Redundancy: 5.5 % / Biso Wilson estimate: 68.25 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.051 / Rpim(I) all: 0.024 / Rrim(I) all: 0.056 / Net I/σ(I): 17.3 / Num. measured all: 198905 / Scaling rejects: 0
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.55-2.665.60.962444443860.5910.4481.0611.8100
8.83-35.15.20.03744058530.9980.0180.04138.596.9

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Processing

Software
NameVersionClassification
PHENIX1.12_2829refinement
Aimless0.5.27data scaling
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: early version of PDB entry 6C2E model

6c2e
PDB Unreleased entry


Resolution: 2.65→35.099 Å / SU ML: 0.46 / Cross valid method: THROUGHOUT / σ(F): 1.92 / Phase error: 30.38
Details: Note uninterpreted electron density near O-gamma of residue Ser-183 of all chains. refmac was used at intermediate stages of refinement. coot was used for interactive model building. A test ...Details: Note uninterpreted electron density near O-gamma of residue Ser-183 of all chains. refmac was used at intermediate stages of refinement. coot was used for interactive model building. A test of swapping chains B and C of the hemimethylated DNA duplex revealed that the diffraction data do not clearly distinguish between alternative strand assignments.
RfactorNum. reflection% reflectionSelection details
Rfree0.2422 2800 4.68 %selected in space group P622, then expanded to P1.
Rwork0.2115 ---
obs0.213 59837 92.04 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 150.24 Å2 / Biso mean: 87.5732 Å2 / Biso min: 54.66 Å2
Refinement stepCycle: final / Resolution: 2.65→35.099 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2982 2922 11 0 5915
Biso mean--92.18 --
Num. residues----552
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.016334
X-RAY DIFFRACTIONf_angle_d1.3169185
X-RAY DIFFRACTIONf_chiral_restr0.073989
X-RAY DIFFRACTIONf_plane_restr0.011694
X-RAY DIFFRACTIONf_dihedral_angle_d26.5783220
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A1711X-RAY DIFFRACTION8.706TORSIONAL
12D1711X-RAY DIFFRACTION8.706TORSIONAL
13G1711X-RAY DIFFRACTION8.706TORSIONAL
14J1711X-RAY DIFFRACTION8.706TORSIONAL
15M1711X-RAY DIFFRACTION8.706TORSIONAL
16P1711X-RAY DIFFRACTION8.706TORSIONAL
21C696X-RAY DIFFRACTION8.706TORSIONAL
22F696X-RAY DIFFRACTION8.706TORSIONAL
23I696X-RAY DIFFRACTION8.706TORSIONAL
24L696X-RAY DIFFRACTION8.706TORSIONAL
25O696X-RAY DIFFRACTION8.706TORSIONAL
26R696X-RAY DIFFRACTION8.706TORSIONAL
31B702X-RAY DIFFRACTION8.706TORSIONAL
32E702X-RAY DIFFRACTION8.706TORSIONAL
33H702X-RAY DIFFRACTION8.706TORSIONAL
34K702X-RAY DIFFRACTION8.706TORSIONAL
35N702X-RAY DIFFRACTION8.706TORSIONAL
36Q702X-RAY DIFFRACTION8.706TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.65-2.69570.34961730.34773064323792
2.6957-2.74470.38251490.35752703285292
2.7447-2.79750.37651360.34512720285692
2.7975-2.85450.36171380.33372932307092
2.8545-2.91660.38741060.32763097320393
2.9166-2.98440.39441190.35162667278692
2.9844-3.0590.29891800.29032944312494
3.059-3.14160.38811000.25932985308594
3.1416-3.2340.26681130.27192674278791
3.234-3.33830.2841870.2662293248072
3.3383-3.45750.27531730.24442455262883
3.4575-3.59580.1716920.2152929302194
3.5958-3.75930.21141080.20472989309794
3.7593-3.95730.30561200.23322956307695
3.9573-4.20480.2409940.19632911300594
4.2048-4.52880.26422170.19333000321795
4.5288-4.98350.21341160.17892891300795
4.9835-5.70190.266770.15973051312896
5.7019-7.17370.19681990.18132885308496
7.1737-35.10240.17752030.16882891309495
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.82793.13322.74395.53872.09755.8675-0.14480.50460.63960.1137-0.0051-0.4465-1.1241.44250.01250.83860.05420.05410.62320.14220.7222-33.4287-22.96661.43
21.32440.35370.03642.24060.08614.45630.89120.9309-0.12040.0214-1.35720.28460.4039-0.0137-0.11710.99340.46980.09080.72330.08551.3047-44.8321-31.9483-4.4027
31.9901-1.2848-0.17716.74270.75267.28890.20320.2648-0.60960.3363-0.28420.75770.0549-0.32970.04430.82710.23360.05150.65740.06131.0314-44.7-32.6638-2.9273
45.5937-2.7043-5.07083.82342.08747.34950.13480.37270.2033-0.2956-0.17210.4291-0.6615-1.19620.05681.0598-0.1745-0.07150.7768-0.0330.5825-50.3714-23.6929-132.1096
52.84364.622-0.74828.019-0.32433.0961-0.35630.1374-0.4499-1.32360.2795-0.80520.24490.15640.13091.3825-0.2343-0.06570.8877-0.06591.078-36.8893-28.6073-139.3195
63.30735.9089-0.52728.0446-0.38364.51220.5141-0.6737-0.1490.4033-0.6511-0.9296-0.47480.47980.10511.238-0.20230.05720.7935-0.01330.9392-36.3892-28.3537-137.8735
77.5148-1.91143.04774.7036-1.39356.85570.1831-0.51090.4861-0.234-0.11610.6725-1.2597-1.37740.13930.8662-0.02380.05110.6376-0.15330.6453-56.6947-14.1024-106.2534
83.70550.99030.00093.24450.7936.76730.5243-0.7085-0.48280.4085-1.1231-0.6279-0.04910.16120.03780.918-0.33450.0430.7426-0.07061.1189-45.4762-23.0893-99.9287
92.31852.26870.01816.6845-1.01447.34090.024-0.3534-0.2691-0.7985-0.2291-0.82260.1998-0.02610.02950.9773-0.26020.09530.6921-0.10951.0896-45.5614-23.8586-101.3905
101.1540.19720.27127.037-6.02389.412-0.0686-0.4985-0.2203-0.21040.11720.11131.37830.2506-0.06250.5579-0.1010.00531.3265-0.03110.5107-43.085-33.0763-64.8109
117.78650.9577-0.41610.58670.46534.7575-0.34061.57861.19660.17130.0917-0.0721-0.02490.5770.05580.7536-0.08090.10621.6431-0.14351.113-45.3706-19.0044-71.9543
128.2201-1.65790.00130.437-0.56274.1673-0.5628-2.01741.37830.38910.0750.4408-0.3828-0.06330.12340.7603-0.1275-0.031.619-0.02390.9742-45.8206-18.7161-70.5017
133.32640.40140.89028.12773.92487.2562-0.18860.2292-0.76640.66880.23950.45152.2248-0.4857-0.01040.601-0.09150.08250.83470.07230.6675-47.8165-43.6777-38.9238
143.44950.0591-0.56071.8164-1.22923.2374-0.8701-1.9061.03170.01860.30610.0454-0.5154-0.5079-0.18790.29210.07950.00181.47660.01521.3075-45.8264-29.2489-32.7519
153.13820.38051.22240.8831-0.03362.6847-1.16781.54050.54440.10540.9350.2-0.67330.1855-0.0561-0.26320.2022-0.02271.74580.22081.3491-45.2115-28.9959-34.2238
166.22823.4972-5.51644.9459-2.07096.40610.4311-0.15880.4191-0.0915-0.2122-0.3731-0.75391.0267-0.14281.06810.2333-0.04460.7006-0.04670.6064-28.4253-25.9636-174.5631
171.1028-3.0582-0.55527.44951.32443.6381-0.4160.0813-0.8731.20810.05510.67130.6589-0.0510.09191.2760.2791-0.07530.91940.02041.1603-41.7745-30.8117-167.015
183.5396-5.6245-0.31888.5573-0.35955.17721.00720.97850.0053-1.4333-1.45090.8248-0.5398-0.59720.13461.41820.2724-0.07120.8984-0.14670.954-42.2918-30.6341-168.4836
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain AA147 - 215
2X-RAY DIFFRACTION2chain BB1 - 12
3X-RAY DIFFRACTION3chain CC1 - 12
4X-RAY DIFFRACTION4chain DD148 - 215
5X-RAY DIFFRACTION5chain EE1 - 12
6X-RAY DIFFRACTION6chain FF1 - 12
7X-RAY DIFFRACTION7chain GG148 - 215
8X-RAY DIFFRACTION8chain HH1 - 12
9X-RAY DIFFRACTION9chain II1 - 12
10X-RAY DIFFRACTION10chain JJ148 - 214
11X-RAY DIFFRACTION11chain KK1 - 12
12X-RAY DIFFRACTION12chain LL1 - 12
13X-RAY DIFFRACTION13chain MM148 - 215
14X-RAY DIFFRACTION14chain NN1 - 12
15X-RAY DIFFRACTION15chain OO1 - 12
16X-RAY DIFFRACTION16chain PP148 - 215
17X-RAY DIFFRACTION17chain QQ1 - 12
18X-RAY DIFFRACTION18chain RR1 - 12

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