PDB-6bit: SIRPalpha antibody complex

ID or keywords:

Entry

Database: PDB / ID: 6bit

Title

SIRPalpha antibody complex

Components

KWAR23 Fab heavy chain
KWAR23 Fab light chain
Tyrosine-protein phosphatase non-receptor type substrate 1

Keywords

IMMUNE SYSTEM /

Macrophage /

Neutrophil /

Phagocytosis / Myeloid /

Immune checkpoint /

CD47 / SIRP /

Cancer immunotherapy

Function / homology

Function and homology information

negative regulation of I-kappaB phosphorylation / cellular response to interleukin-12 / monocyte extravasation / negative regulation of macrophage inflammatory protein 1 alpha production / negative regulation of chemokine (C-C motif) ligand 5 production / protein binding involved in heterotypic cell-cell adhesion / regulation of interleukin-1 beta production / regulation of type II interferon production / cell-cell adhesion mediator activity / GTPase regulator activity ...
Similarity search - Function

Biological species

Homo sapiens (human)

Mus musculus (house mouse)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.191 Å

Authors

Ring, N.G. / Herndler-Brandstetter, D. / Weiskopf, K. / Shan, L. / Volkmer, J.P. / George, B.M. / Lietzenmayer, M. / McKenna, K.M. / Naik, T.J. / McCarty, A. ...

Funding support

United States, 3items

Citation

Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2017
Title: Anti-SIRP alpha antibody immunotherapy enhances neutrophil and macrophage antitumor activity.
Authors: Ring, N.G. / Herndler-Brandstetter, D. / Weiskopf, K. / Shan, L. / Volkmer, J.P. / George, B.M. / Lietzenmayer, M. / McKenna, K.M. / Naik, T.J. / McCarty, A. / Zheng, Y. / Ring, A.M. / ...

History

Deposition	Nov 3, 2017	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 6, 2017	Provider: repository / Type: Initial release
Revision 1.1	Dec 13, 2017	Group: Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2	Jan 17, 2018	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3	Nov 20, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	6bit.cif.gz	422.6 KB	Display	PDBx/mmCIF format
PDB format	pdb6bit.ent.gz	360.1 KB	Display	PDB format
PDBx/mmJSON format	6bit.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/bi/6bit ftp://data.pdbj.org/pub/pdb/validation_reports/bi/6bit	HTTPS FTP

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Related structure data

Similar structure data	5urb-1 7bxa-2 6was-1 6bfq-4 4ffz-2 1r8k-d 6pe9-4 2xrc-2 3skj-2 5otj-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#21 - Sep 2001 Antibodies similarity (1) #256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1) #43 - Jul 2003 Src Tyrosine Kinase similarity (1) #60 - Dec 2004 Ubiquitin similarity (1)

Deposited unit

I: KWAR23 Fab heavy chain

K: KWAR23 Fab light chain

H: Tyrosine-protein phosphatase non-receptor type substrate 1

J: KWAR23 Fab heavy chain

L: KWAR23 Fab light chain

G: Tyrosine-protein phosphatase non-receptor type substrate 1

118 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

I: KWAR23 Fab heavy chain

K: KWAR23 Fab light chain

H: Tyrosine-protein phosphatase non-receptor type substrate 1

defined by author
Evidence: gel filtration
58.8 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

J: KWAR23 Fab heavy chain

L: KWAR23 Fab light chain

G: Tyrosine-protein phosphatase non-receptor type substrate 1

defined by author
58.8 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	164.966, 164.966, 96.716
Angle α, β, γ (deg.)	90.00, 90.00, 120.00
Int Tables number	154
Space group name H-M	P3₂21

#1: Antibody	KWAR23 Fab heavy chain Mass: 22923.551 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Tissue: Hybridoma supernatant
#2: Antibody	KWAR23 Fab light chain Mass: 23068.410 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Tissue: Hybridoma supernatant
#3: Protein	Tyrosine-protein phosphatase non-receptor type substrate 1 / SHPS-1 / Brain Ig-like molecule with tyrosine-based activation motifs / Bit / CD172 antigen-like ...SHPS-1 / Brain Ig-like molecule with tyrosine-based activation motifs / Bit / CD172 antigen-like family member A / Inhibitory receptor SHPS-1 / Macrophage fusion receptor / MyD-1 antigen / Signal-regulatory protein alpha-1 / Sirp-alpha-1 / Signal-regulatory protein alpha-2 / Sirp-alpha-2 / Signal-regulatory protein alpha-3 / Sirp-alpha-3 / p84 Mass: 12803.461 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP / Production host: Escherichia coli (E. coli) / References: UniProt: P78324
#4: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 245 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.22 Å³/Da / Density % sol: 61.84 %
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Crystals were obtained by addition of 0.1 uL of protein to an equal volume of 22% PEG3350, 150 mM ammonium sulfate, and 0.1 M Tris (pH 7.0)

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Data collection

Diffraction	Mean temperature: 100 K / Ambient temp details: Liquid nitrogen cryostream
Diffraction source	Source: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 24, 2013
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.19→47.622 Å / Num. obs: 76976 / % possible obs: 99.2 % / Redundancy: 6 % / Net I/σ(I): 21.5
Reflection shell	Resolution: 2.19→2.27 Å / Redundancy: 4.3 % / Num. unique obs: 6916 / % possible all: 87.9

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Processing

Software

Name	Version	Classification
PHENIX	1.11.1_2575	refinement
HKL-2000		data reduction
SCALA		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.191→47.622 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 26.02

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2402	3856	5.01 %	0.05
R_work	0.2103	-	-	-
obs	0.2118	76965	98.79 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2.191→47.622 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	8147	0	0	245	8392

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	8381
X-RAY DIFFRACTION	f_angle_d	0.531	11439
X-RAY DIFFRACTION	f_dihedral_angle_d	12.239	5011
X-RAY DIFFRACTION	f_chiral_restr	0.043	1296
X-RAY DIFFRACTION	f_plane_restr	0.004	1451

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.191-2.2178	0.3562	97	0.3055	1858	X-RAY DIFFRACTION	71
2.2178-2.2458	0.3383	134	0.288	2617	X-RAY DIFFRACTION	99
2.2458-2.2754	0.3107	141	0.2868	2613	X-RAY DIFFRACTION	100
2.2754-2.3066	0.3018	137	0.277	2636	X-RAY DIFFRACTION	100
2.3066-2.3395	0.2926	134	0.2765	2600	X-RAY DIFFRACTION	100
2.3395-2.3744	0.312	140	0.2695	2619	X-RAY DIFFRACTION	100
2.3744-2.4115	0.2899	135	0.2549	2631	X-RAY DIFFRACTION	100
2.4115-2.4511	0.3379	139	0.2657	2613	X-RAY DIFFRACTION	100
2.4511-2.4933	0.2945	137	0.2602	2627	X-RAY DIFFRACTION	100
2.4933-2.5387	0.3019	137	0.2635	2608	X-RAY DIFFRACTION	100
2.5387-2.5875	0.2858	140	0.267	2615	X-RAY DIFFRACTION	100
2.5875-2.6403	0.2997	139	0.2596	2603	X-RAY DIFFRACTION	100
2.6403-2.6977	0.2989	141	0.2669	2684	X-RAY DIFFRACTION	100
2.6977-2.7605	0.3044	135	0.264	2609	X-RAY DIFFRACTION	100
2.7605-2.8295	0.3191	138	0.2607	2631	X-RAY DIFFRACTION	100
2.8295-2.906	0.2796	139	0.2524	2603	X-RAY DIFFRACTION	100
2.906-2.9915	0.2701	146	0.247	2653	X-RAY DIFFRACTION	100
2.9915-3.088	0.2785	136	0.2339	2617	X-RAY DIFFRACTION	100
3.088-3.1984	0.2791	138	0.2304	2624	X-RAY DIFFRACTION	100
3.1984-3.3264	0.2818	141	0.2265	2660	X-RAY DIFFRACTION	100
3.3264-3.4777	0.2385	140	0.2098	2657	X-RAY DIFFRACTION	100
3.4777-3.661	0.2376	138	0.2026	2635	X-RAY DIFFRACTION	100
3.661-3.8903	0.2151	141	0.1861	2643	X-RAY DIFFRACTION	100
3.8903-4.1905	0.2025	138	0.1755	2662	X-RAY DIFFRACTION	100
4.1905-4.6119	0.1658	143	0.1572	2657	X-RAY DIFFRACTION	100
4.6119-5.2785	0.1694	139	0.1511	2674	X-RAY DIFFRACTION	100
5.2785-6.6475	0.2078	141	0.1793	2690	X-RAY DIFFRACTION	99
6.6475-47.6328	0.2045	152	0.1865	2770	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	9.5879	-2.1625	2.8711	2.5074	-2.5587	3.6188	-0.1173	1.1292	-0.6052	-0.0278	0.2336	-1.2774	0.2183	0.6855	-0.1383	0.5047	0.045	-0.1335	0.6687	-0.2925	1.1577	-44.9758	17.9872	-8.9047
2	6.1797	-1.4394	0.539	6.6179	0.5626	3.4449	-0.1475	0.0545	-0.3102	0.3019	0.4664	-1.0524	-0.1689	0.5099	-0.1514	0.3666	0.0084	-0.0532	0.4234	-0.1286	0.6509	-54.8043	25.3292	-5.6226
3	0.8421	-1.901	0.0482	3.1435	-0.6929	-0.1484	-0.1831	-0.17	0.2693	-0.0836	-0.0368	-0.8801	0.017	0.0186	0.1645	0.4625	-0.0238	0.0073	0.4982	-0.0515	1.0078	-43.6264	5.5961	-9.3688
4	2.6717	0.9092	0.2564	5.7434	1.4503	1.8884	-0.2881	0.2344	0.3248	-0.9056	0.0779	-0.5411	-0.0727	0.2632	0.1765	0.5068	-0.052	0.1218	0.4404	0.0757	0.8616	-39.3276	-6.9544	-14.6148
5	1.0552	-2.7749	-0.0643	3.355	-1.5521	0.3733	-0.1179	-0.0802	-0.6389	0.1082	0.3139	-0.0889	-0.0088	-0.0162	-0.143	0.3541	0.0342	-0.1149	0.3699	-0.0288	0.7735	-64.8857	4.2104	-3.772
6	6.7157	2.8388	-0.9766	4.1398	0.0201	2.3699	-0.1053	-0.3946	0.3435	-0.0971	-0.2429	-0.7204	-0.1418	0.4576	0.3314	0.3846	0.0028	0.0038	0.3901	0.0693	0.74	-43.0825	-16.4432	0.1462
7	9.0125	5.3955	0.8987	6.0427	-0.2964	8.8769	0.2937	-0.1314	0.7542	0.0995	-0.3823	0.6406	-0.5719	0.1572	0.1505	0.4073	0.0845	0.03	0.2628	0.0006	0.3182	-81.2991	43.4572	-12.7847
8	4.302	-0.1754	1.1594	1.9531	0.0084	1.2862	-0.0226	-0.2113	-0.1598	0.0239	0.0716	-0.1097	0.0794	0.0906	-0.0391	0.38	0.0283	0.014	0.2806	-0.0087	0.2381	-77.8402	30.1899	-13.301
9	6.6937	5.6144	-2.0934	8.086	-1.9753	5.3814	-0.28	0.1051	-0.7906	-0.4292	0.1971	-0.9851	-0.0306	0.37	0.0309	0.3723	0.092	0.017	0.3031	-0.01	0.3844	-76.6077	31.106	-19.6491
10	6.8954	3.2117	2.2685	1.9868	1.6865	2.2667	0.0703	-0.3326	-0.224	0.1914	0.003	-0.2881	-0.039	0.1244	0.0317	0.4588	0.0591	-0.0556	0.3407	-0.0011	0.328	-75.0293	35.9759	-5.2348
11	5.1301	5.129	4.1894	5.4398	3.6134	7.5802	0.4101	-0.3325	-0.5531	0.5572	-0.1134	0.8604	0.7298	-0.2897	-0.2556	0.3977	0.0159	-0.0163	0.353	0.0519	0.5171	-93.0541	23.8905	-16.7615
12	1.5518	0.9032	-0.8252	3.3743	-1.9373	3.3518	-0.0456	0.0677	-0.0748	-0.0981	0.0126	0.0595	0.0853	-0.1942	0.0107	0.2091	0.0139	-0.0102	0.297	0.0014	0.2386	-65.5718	-43.5073	-4.7648
13	0.3886	-0.7483	-0.6142	7.2201	-0.8206	9.3224	0.126	-0.1606	0.6241	-0.1718	0.1289	-0.4377	-0.8311	0.973	-0.2566	0.3196	-0.0206	-0.0169	0.3548	-0.057	0.3039	-60.9826	-34.7701	0.9745
14	7.1485	5.5094	-1.6738	9.5753	-0.9496	3.6087	0.0398	-0.0021	0.6529	0.1239	0.0149	0.0401	-0.5323	-0.3321	-0.1541	0.311	0.0812	-0.0308	0.3123	-0.0419	0.3487	-71.1246	-34.2128	3.7658
15	2.7829	0.0452	-0.6655	0.8166	-0.2693	1.6653	0.1148	0.399	-0.0301	-0.1703	-0.0863	-0.0405	0.154	0.1275	-0.0318	0.3543	0.0588	-0.0327	0.3839	-0.0387	0.311	-57.69	-40.012	-13.8032
16	7.2152	-0.1792	-1.6558	1.4748	1.3101	4.871	0.365	0.663	-0.7678	0.0285	-0.1593	0.0232	0.6093	0.4632	-0.1337	0.4331	0.089	-0.0768	0.5141	-0.0525	0.3782	-45.6568	-42.6312	-21.9672
17	6.7558	-0.9104	-2.7606	4.6196	0.6121	5.4804	-0.2802	0.8389	-1.0636	-0.7412	0.1383	0.294	1.1595	0.2943	0.1836	0.9132	0.1832	-0.2039	0.6682	-0.1967	0.6376	-45.3625	-51.2387	-29.7637
18	3.08	-2.9506	-4.5508	3.6758	3.5316	7.7953	0.6364	-1.1591	0.4348	-0.4201	-0.0325	-0.3098	-0.663	1.1543	-0.6163	0.4246	-0.1019	0.0384	0.4751	-0.0754	0.3673	-41.7117	-31.75	6.4771
19	3.8237	1.2325	-1.2365	2.7077	-0.7374	2.2876	0.1563	-0.4956	-0.0411	0.2483	-0.3185	-0.0684	-0.0256	0.3739	0.1063	0.2297	-0.0141	-0.0213	0.4407	0.0171	0.2847	-45.9259	-41.5221	10.3951
20	5.4871	1.1407	-0.4717	0.7204	-0.5044	5.5366	0.1335	0.1188	-0.1941	-0.0174	-0.283	-0.2692	0.1265	0.452	0.07	0.279	-0.0261	0.009	0.4098	0.0343	0.2964	-41.9409	-41.7752	4.5293
21	5.1251	0.4883	0.5907	1.7636	-0.4598	0.272	-0.2343	-0.2839	-0.1154	-0.1276	0.0268	-0.0135	0.153	-0.1725	0.1759	0.3257	-0.0205	0.0573	0.3154	-0.0821	0.3505	-58.1559	-34.1415	9.6099
22	5.3634	-1.2009	1.7316	1.8042	1.4053	2.7512	0.2701	0.3475	0.4621	-0.3276	-0.0628	-0.3834	-0.4124	0.9338	-0.0461	0.3556	0.0841	0.0769	0.5883	0.1676	0.3985	-30.7331	-38.7586	-5.0495
23	1.4766	0.421	0.4365	5.2021	-2.3101	1.2797	0.0523	0.7683	-0.4854	-1.3559	-0.1177	-0.1486	0.9002	-0.0681	0.2264	0.8189	0.3272	0.0031	1.3514	-0.037	0.409	-38.5263	-40.0733	-35.0943
24	0.5635	-0.9986	1.8643	2.5242	-2.7557	6.6326	0.2114	1.2567	0.3713	-0.3213	-0.5893	-0.383	-0.3309	0.7819	0.4073	0.505	0.1923	0.0895	1.0874	0.2157	0.5567	-31.6754	-34.4714	-22.453
25	8.623	2.8271	-7.2108	4.8855	-2.4306	8.6651	0.5816	1.3318	1.6813	-0.3342	0.1392	-0.2384	-0.8808	-0.2477	-0.6416	0.5646	0.0327	0.0704	0.872	0.2946	0.6729	-34.5476	-27.5837	-27.2289
26	5.8163	-3.1481	2.8154	7.9794	-5.0731	9.6055	0.0008	-0.2758	-0.9393	0.1067	0.768	1.5717	0.5932	-0.0019	-0.9333	0.3802	0.037	0.0122	0.5794	0.1297	0.6117	-35.281	-42.6058	-9.8726
27	4.8407	1.4267	-3.045	4.6538	2.786	5.0864	-0.7025	0.9833	0.4632	-1.2408	-0.5828	-0.2668	-0.4522	-0.3615	0.9869	0.787	0.0695	0.1259	1.066	0.2604	0.5777	-38.1866	-31.3013	-36.3607
28	1.1357	-0.3932	1.5869	2.6129	-1.7053	2.7693	0.3904	1.8154	0.3663	-1.0519	-0.2026	-0.4805	-0.416	0.8453	0.1824	0.7109	0.1695	0.2287	1.441	0.3317	0.5237	-25.4533	-34.0398	-28.5251
29	5.9537	-5.5115	6.361	5.3079	-5.9284	7.5843	-0.3276	-0.7753	0.2564	0.9777	0.2963	0.1663	-0.3982	-0.1312	-0.0208	0.3643	-0.0557	0.0253	0.3821	-0.0375	0.3325	-74.796	-51.4368	30.8499
30	7.2855	2.5931	2.1715	2.2894	-2.0902	6.6808	-0.1964	-0.7225	-1.2247	0.3156	-0.0045	-0.5859	0.6317	0.8466	0.2824	0.3916	0.1106	-0.0414	0.472	0.1081	0.4904	-57.622	-62.1023	27.9445
31	5.1419	1.7218	-0.9648	4.7552	-1.4414	2.361	0.0696	0.1612	0.0356	-0.1625	0.0066	-0.1496	0.156	-0.0815	-0.1019	0.2243	0.0162	0.0027	0.2714	-0.0346	0.162	-71.8825	-49.9907	22.6251
32	7.3441	0.5304	1.6449	2.9335	2.6706	2.9515	-0.0669	-0.3028	0.7244	0.3226	0.0998	-0.0107	-0.273	0.6223	-0.3904	0.3852	-0.0941	0.0394	0.3556	-0.008	0.387	-60.4503	-44.7347	23.1158
33	2.5535	0.4303	-0.1521	1.7288	-0.4922	1.9834	0.0169	-0.0131	-0.2541	-0.0749	-0.0156	-0.1557	0.1409	0.07	0.0494	0.2694	0.0298	0.0199	0.3176	-0.0333	0.2745	-64.9795	-55.4918	17.7797
34	3.4712	-1.9827	4.624	2.7604	-2.4884	8.3911	-0.0782	-0.943	-0.1143	0.0152	0.1248	0.1249	0.2073	-0.4677	-0.1523	0.2972	-0.0302	-0.0083	0.3173	-0.0481	0.2645	-67.2369	-46.7954	25.3533
35	1.1299	-1.258	1.1329	4.2457	-5.3938	7.2869	0.205	-0.0111	0.2266	0.139	-0.4578	-0.1874	-0.3681	0.6648	0.0288	0.4227	-0.038	0.0311	0.3499	-0.0679	0.3253	-71.79	-40.4348	21.5918
36	6.3317	-6.0371	-0.179	6.1596	-0.4328	7.9909	-0.8304	-1.8711	-0.43	1.3782	0.0961	-1.0853	0.5655	0.1706	0.4555	0.4126	-0.0666	-0.0658	0.6119	0.0893	0.6911	-55.5094	-58.761	32.725

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'I' and (resid 1 through 17 )
2	X-RAY DIFFRACTION	2	chain 'I' and (resid 18 through 73 )
3	X-RAY DIFFRACTION	3	chain 'I' and (resid 74 through 157 )
4	X-RAY DIFFRACTION	4	chain 'I' and (resid 158 through 213 )
5	X-RAY DIFFRACTION	5	chain 'K' and (resid 2 through 128 )
6	X-RAY DIFFRACTION	6	chain 'K' and (resid 129 through 212 )
7	X-RAY DIFFRACTION	7	chain 'H' and (resid 1 through 11 )
8	X-RAY DIFFRACTION	8	chain 'H' and (resid 12 through 62 )
9	X-RAY DIFFRACTION	9	chain 'H' and (resid 63 through 86 )
10	X-RAY DIFFRACTION	10	chain 'H' and (resid 87 through 109 )
11	X-RAY DIFFRACTION	11	chain 'H' and (resid 110 through 117 )
12	X-RAY DIFFRACTION	12	chain 'J' and (resid 1 through 39 )
13	X-RAY DIFFRACTION	13	chain 'J' and (resid 40 through 52 )
14	X-RAY DIFFRACTION	14	chain 'J' and (resid 53 through 67 )
15	X-RAY DIFFRACTION	15	chain 'J' and (resid 68 through 145 )
16	X-RAY DIFFRACTION	16	chain 'J' and (resid 146 through 184 )
17	X-RAY DIFFRACTION	17	chain 'J' and (resid 185 through 213 )
18	X-RAY DIFFRACTION	18	chain 'L' and (resid 2 through 14 )
19	X-RAY DIFFRACTION	19	chain 'L' and (resid 15 through 75 )
20	X-RAY DIFFRACTION	20	chain 'L' and (resid 76 through 90 )
21	X-RAY DIFFRACTION	21	chain 'L' and (resid 91 through 101 )
22	X-RAY DIFFRACTION	22	chain 'L' and (resid 102 through 113 )
23	X-RAY DIFFRACTION	23	chain 'L' and (resid 114 through 128 )
24	X-RAY DIFFRACTION	24	chain 'L' and (resid 129 through 150 )
25	X-RAY DIFFRACTION	25	chain 'L' and (resid 151 through 163 )
26	X-RAY DIFFRACTION	26	chain 'L' and (resid 164 through 174 )
27	X-RAY DIFFRACTION	27	chain 'L' and (resid 175 through 188 )
28	X-RAY DIFFRACTION	28	chain 'L' and (resid 189 through 211 )
29	X-RAY DIFFRACTION	29	chain 'G' and (resid 2 through 11 )
30	X-RAY DIFFRACTION	30	chain 'G' and (resid 12 through 23 )
31	X-RAY DIFFRACTION	31	chain 'G' and (resid 24 through 40 )
32	X-RAY DIFFRACTION	32	chain 'G' and (resid 41 through 51 )
33	X-RAY DIFFRACTION	33	chain 'G' and (resid 52 through 86 )
34	X-RAY DIFFRACTION	34	chain 'G' and (resid 87 through 95 )
35	X-RAY DIFFRACTION	35	chain 'G' and (resid 96 through 109 )
36	X-RAY DIFFRACTION	36	chain 'G' and (resid 110 through 116 )

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