Tyrosine-protein phosphatase non-receptor type substrate 1
Keywords
IMMUNE SYSTEM / Macrophage / Neutrophil / Phagocytosis / Myeloid / Immune checkpoint / CD47 / SIRP / Cancer immunotherapy
Function / homology
Function and homology information
negative regulation of I-kappaB phosphorylation / cellular response to interleukin-12 / monocyte extravasation / negative regulation of macrophage inflammatory protein 1 alpha production / negative regulation of chemokine (C-C motif) ligand 5 production / protein binding involved in heterotypic cell-cell adhesion / regulation of interleukin-1 beta production / regulation of type II interferon production / GTPase regulator activity / cell-cell adhesion mediator activity ...negative regulation of I-kappaB phosphorylation / cellular response to interleukin-12 / monocyte extravasation / negative regulation of macrophage inflammatory protein 1 alpha production / negative regulation of chemokine (C-C motif) ligand 5 production / protein binding involved in heterotypic cell-cell adhesion / regulation of interleukin-1 beta production / regulation of type II interferon production / GTPase regulator activity / cell-cell adhesion mediator activity / protein antigen binding / negative regulation of nitric oxide biosynthetic process / negative regulation of interferon-beta production / negative regulation of JNK cascade / regulation of tumor necrosis factor production / regulation of nitric oxide biosynthetic process / negative regulation of phagocytosis / regulation of interleukin-6 production / Signal regulatory protein family interactions / negative regulation of interleukin-6 production / tertiary granule membrane / ficolin-1-rich granule membrane / negative regulation of tumor necrosis factor production / negative regulation of cytokine production involved in inflammatory response / cellular response to interleukin-1 / positive regulation of phagocytosis / protein tyrosine kinase binding / negative regulation of protein phosphorylation / Cell surface interactions at the vascular wall / negative regulation of ERK1 and ERK2 cascade / negative regulation of inflammatory response / cellular response to type II interferon / SH3 domain binding / cellular response to hydrogen peroxide / cell migration / positive regulation of T cell activation / regulation of gene expression / cellular response to lipopolysaccharide / protein phosphatase binding / cell adhesion / Neutrophil degranulation / cell surface / extracellular exosome / membrane / plasma membrane Similarity search - Function
I: KWAR23 Fab heavy chain K: KWAR23 Fab light chain H: Tyrosine-protein phosphatase non-receptor type substrate 1 J: KWAR23 Fab heavy chain L: KWAR23 Fab light chain G: Tyrosine-protein phosphatase non-receptor type substrate 1
Mass: 18.015 Da / Num. of mol.: 245 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.22 Å3/Da / Density % sol: 61.84 %
Crystal grow
Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Crystals were obtained by addition of 0.1 uL of protein to an equal volume of 22% PEG3350, 150 mM ammonium sulfate, and 0.1 M Tris (pH 7.0)
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Data collection
Diffraction
Mean temperature: 100 K / Ambient temp details: Liquid nitrogen cryostream