ProteinAF-9 / ALL1-fused gene from chromosome 9 protein / Myeloid/lymphoid or mixed-lineage leukemia translocated ...ALL1-fused gene from chromosome 9 protein / Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein / YEATS domain-containing protein 3
Mass: 8278.414 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MLLT3, AF9, YEATS3 / Production host: Escherichia coli (E. coli) / References: UniProt: P42568
#2: Protein/peptide
BCL-6corepressor / BCoR
Mass: 3713.134 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BCOR, KIAA1575 / Production host: Escherichia coli (E. coli) / References: UniProt: Q6W2J9
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
1
isotropic
2
2D 1H-13C HSQC
1
3
1
isotropic
1
3D HNCO
1
4
1
isotropic
1
3D HNHA
1
5
1
isotropic
1
3D HN(CA)CB
1
11
1
isotropic
1
3DCBCA(CO)NH
1
10
1
isotropic
2
3D (H)CCH-TOCSY
1
9
1
isotropic
3
3DC(CO)NH
1
8
1
isotropic
2
3D 1H-13C NOESY aliphatic
1
7
1
isotropic
3
3D 1H-13C NOESY aromatic
1
6
1
isotropic
1
3D 1H-15N NOESY
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Sample preparation
Details
Type: solution Contents: 750 uM [U-13C; U-15N] BCoR, 750 uM [U-13C; U-15N] AF9, 9.3 mM Bis-Tris, 15.8 mM MES, 100 mM NaCl, 1 mM DTT, 5 % [U-99% 2H] D2O, 95 % H2O, 95% H2O/5% D2O Label: BCoR-AF9 / Solvent system: 95% H2O/5% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
750uM
BCoR
[U-13C; U-15N]
1
750uM
AF9
[U-13C; U-15N]
1
9.3mM
Bis-Tris
naturalabundance
1
15.8mM
MES
naturalabundance
1
100mM
NaCl
naturalabundance
1
1mM
DTT
naturalabundance
1
5 %
D2O
[U-99% 2H]
1
95 %
H2O
naturalabundance
1
Sample conditions
Ionic strength: 100 mM / Label: BCoR-AF9 / pH: 6.0 / Pressure: ambient / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AVANCE
Bruker
AVANCE
600
1
Bruker AVANCE
Bruker
AVANCE
800
2
Varian INOVA
Varian
INOVA
600
3
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Processing
NMR software
Name
Developer
Classification
TopSpin
BrukerBiospin
collection
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
processing
Analysis
CCPN
peakpicking
CYANA
Guntert, MumenthalerandWuthrich
chemicalshiftassignment
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
structurecalculation
VNMR
Varian
collection
MOLMOL
Koradi, BilleterandWuthrich
dataanalysis
PyMOL
Schroedinger
dataanalysis
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
refinement
Refinement
Method: simulated annealing / Software ordinal: 5
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10
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