+Open data
-Basic information
Entry | Database: PDB / ID: 6a5b | ||||||
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Title | Crystal structure of plant Receptor-like Kinase FER | ||||||
Components | Receptor-like protein kinase FERONIA | ||||||
Keywords | TRANSFERASE / a plant receptor F | ||||||
Function / homology | Function and homology information pollen tube reception / filiform apparatus / negative regulation of abscisic acid-activated signaling pathway / response to brassinosteroid / response to ethylene / brassinosteroid mediated signaling pathway / ethylene-activated signaling pathway / stomatal movement / root development / plasmodesma ...pollen tube reception / filiform apparatus / negative regulation of abscisic acid-activated signaling pathway / response to brassinosteroid / response to ethylene / brassinosteroid mediated signaling pathway / ethylene-activated signaling pathway / stomatal movement / root development / plasmodesma / abscisic acid-activated signaling pathway / defense response to fungus / transmembrane receptor protein tyrosine kinase activity / post-embryonic development / circadian regulation of gene expression / negative regulation of cell growth / protein autophosphorylation / non-specific serine/threonine protein kinase / protein kinase activity / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.205 Å | ||||||
Authors | Xiao, Y. / Chai, J. | ||||||
Citation | Journal: Nature / Year: 2019 Title: Mechanisms of RALF peptide perception by a heterotypic receptor complex. Authors: Xiao, Y. / Stegmann, M. / Han, Z. / DeFalco, T.A. / Parys, K. / Xu, L. / Belkhadir, Y. / Zipfel, C. / Chai, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6a5b.cif.gz | 88.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6a5b.ent.gz | 64.9 KB | Display | PDB format |
PDBx/mmJSON format | 6a5b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6a5b_validation.pdf.gz | 426.9 KB | Display | wwPDB validaton report |
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Full document | 6a5b_full_validation.pdf.gz | 430.5 KB | Display | |
Data in XML | 6a5b_validation.xml.gz | 15.4 KB | Display | |
Data in CIF | 6a5b_validation.cif.gz | 21.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a5/6a5b ftp://data.pdbj.org/pub/pdb/validation_reports/a5/6a5b | HTTPS FTP |
-Related structure data
Related structure data | 6a5aC 6a5cSC 6a5dC 6a5eC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46815.863 Da / Num. of mol.: 1 / Fragment: UNP residues 21-450 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: FER, AAK1, SIR, SRN, At3g51550, F26O13.190 Production host: Insect cell expression vector pTIE1 (others) References: UniProt: Q9SCZ4, non-specific serine/threonine protein kinase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.42 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 0.1M Bis-Tris pH 6.5, 25%(w/v) Polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 1 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 1, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 21471 / % possible obs: 98.2 % / Redundancy: 7 % / Rsym value: 0.075 / Net I/σ(I): 20.4 |
Reflection shell | Resolution: 2.2→2.28 Å / Num. unique obs: 2099 / Rsym value: 0.642 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6A5C Resolution: 2.205→40.595 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 23.56 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Displacement parameters | Biso max: 89.27 Å2 / Biso mean: 42.9336 Å2 / Biso min: 22.39 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.205→40.595 Å
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