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- PDB-1osb: Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free st... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1osb | ||||||
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Title | Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure. | ||||||
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![]() | TRANSFERASE/DNA / Bacterial conjugation / Relaxase / DNA replication / TRANSFERASE-DNA COMPLEX | ||||||
Function / homology | ![]() G-quadruplex DNA unwinding / mitochondrial genome maintenance / replication fork reversal / telomere maintenance / single-stranded DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guasch, A. / Lucas, M. / Moncalian, G. / Cabezas, M. / Perez-Luque, R. / Gomis-Ruth, F.X. / de la Cruz, F. / Coll, M. | ||||||
![]() | ![]() Title: Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC. Authors: Guasch, A. / Lucas, M. / Moncalian, G. / Cabezas, M. / Perez-Luque, R. / Gomis-Ruth, F.X. / de la Cruz, F. / Coll, M. #1: ![]() Title: Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation Authors: Grandoso, G. / Avila, P. / Cayon, A. / Hernando, M.A. / Llosa, M. / de la Cruz, F. #2: ![]() Title: Bacterial conjugation: a two-step mechanism for DNA transport. Authors: Llosa, M. / Gomis-Ruth, F.X. / Coll, M. / de la Cruz, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 157.8 KB | Display | ![]() |
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PDB format | ![]() | 121.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1omhSC ![]() 1qx0C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 7714.970 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA forming a cruciform arm #2: Protein | Mass: 32894.832 Da / Num. of mol.: 2 / Fragment: N-terminal relaxase domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.03 Å3/Da / Density % sol: 59.41 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296KK | ||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 4 ℃ | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MACSCIENCE / Detector: CCD / Date: Feb 20, 2002 |
Radiation | Monochromator: diamond / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.65→40 Å / Num. obs: 28480 / % possible obs: 99.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4.4 % / Rmerge(I) obs: 0.098 / Net I/σ(I): 7 |
Reflection shell | Highest resolution: 2.65 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.291 / Mean I/σ(I) obs: 2.5 / % possible all: 97.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1OMH Resolution: 2.65→40 Å / Cross valid method: FREE R-VALUE / σ(F): 1 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.65→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.65→2.719 Å /
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