[English] 日本語
![](img/lk-miru.gif)
- PDB-1omh: Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free st... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1omh | ||||||
---|---|---|---|---|---|---|---|
Title | Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure. | ||||||
![]() |
| ||||||
![]() | TRANSFERASE/DNA / PROTEIN-DNA COMPLEX / BACTERIAL CONJUGATION / RELAXASE / DNA REPLICATION / TRANSFERASE-DNA COMPLEX | ||||||
Function / homology | ![]() G-quadruplex DNA unwinding / mitochondrial genome maintenance / replication fork reversal / telomere maintenance / single-stranded DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guasch, A. / Lucas, M. / Moncalian, G. / Cabezas, M. / Perez-Luque, R. / Gomis-Ruth, F.X. / de la Cruz, F. / Coll, M. | ||||||
![]() | ![]() Title: Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC. Authors: Guasch, A. / Lucas, M. / Moncalian, G. / Cabezas, M. / Perez-Luque, R. / Gomis-Ruth, F.X. / de la Cruz, F. / Coll, M. #1: ![]() Title: Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation Authors: Grandoso, G. / Avila, P. / Cayon, A. / Hernando, M.A. / Llosa, M. / de la Cruz, F. #2: ![]() Title: Bacterial conjugation: a two-step mechanism for DNA transport. Authors: Llosa, M. / Gomis-Ruth, F.X. / Coll, M. / de la Cruz, F. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 84.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 67.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: DNA chain | Mass: 7714.970 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: DNA FORMING A CRUCIFORM ARM | ||
---|---|---|---|
#2: Protein | Mass: 33082.414 Da / Num. of mol.: 1 / Fragment: N-terminal relaxase domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.3 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K | ||||||||||||||||||||||||||||||
Components of the solutions |
| ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source |
| |||||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 8, 2002 | |||||||||||||||
Radiation | Monochromator: diamond / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
| |||||||||||||||
Reflection | Resolution: 2.05→50 Å / Num. obs: 64999 / % possible obs: 100 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Biso Wilson estimate: 29.69 Å2 / Rmerge(I) obs: 0.146 | |||||||||||||||
Reflection shell | Resolution: 1.95→50 Å / % possible all: 100 |
-
Processing
Software |
| |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→50 Å
| |||||||||||||||||||||
Refine LS restraints |
|