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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 5z8y | |||||||||
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タイトル | Crystal structure of human LRRTM2 in complex with Neurexin 1beta | |||||||||
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![]() | CELL ADHESION / Synapse / leucine-rich repeat / synaptic organizer | |||||||||
機能・相同性 | ![]() protein-containing complex assembly involved in synapse maturation / positive regulation of presynaptic active zone assembly / cell-cell adhesion involved in synapse maturation / protein complex involved in cell-cell adhesion / gephyrin clustering involved in postsynaptic density assembly / type 1 fibroblast growth factor receptor binding / positive regulation of neuromuscular synaptic transmission / guanylate kinase-associated protein clustering / neuron to neuron synapse / neuronal signal transduction ...protein-containing complex assembly involved in synapse maturation / positive regulation of presynaptic active zone assembly / cell-cell adhesion involved in synapse maturation / protein complex involved in cell-cell adhesion / gephyrin clustering involved in postsynaptic density assembly / type 1 fibroblast growth factor receptor binding / positive regulation of neuromuscular synaptic transmission / guanylate kinase-associated protein clustering / neuron to neuron synapse / neuronal signal transduction / neuroligin clustering involved in postsynaptic membrane assembly / postsynaptic density protein 95 clustering / cerebellar granule cell differentiation / postsynaptic membrane assembly / synapse maturation / presynaptic membrane assembly / gamma-aminobutyric acid receptor clustering / negative regulation of filopodium assembly / maintenance of synapse structure / neuroligin family protein binding / positive regulation of fibroblast growth factor receptor signaling pathway / regulation of postsynaptic density assembly / inhibitory synapse / synaptic vesicle clustering / neuron cell-cell adhesion / neurexin family protein binding / receptor localization to synapse / protein localization to synapse / NMDA glutamate receptor clustering / vocalization behavior / calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules / regulation of AMPA receptor activity / negative regulation of receptor internalization / postsynaptic specialization membrane / Neurexins and neuroligins / positive regulation of synapse assembly / regulation of NMDA receptor activity / positive regulation of protein kinase C activity / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / adult behavior / positive regulation of protein kinase A signaling / positive regulation of excitatory postsynaptic potential / social behavior / excitatory synapse / endocytic vesicle / GABA-ergic synapse / axonal growth cone / cell adhesion molecule binding / synapse assembly / cellular response to calcium ion / hippocampal mossy fiber to CA3 synapse / positive regulation of synaptic transmission, glutamatergic / neuron projection morphogenesis / learning / positive regulation of synaptic transmission, GABAergic / long-term synaptic potentiation / positive regulation of protein localization to plasma membrane / postsynaptic density membrane / synapse organization / Schaffer collateral - CA1 synapse / establishment of protein localization / neuromuscular junction / positive regulation of neuron projection development / transmembrane signaling receptor activity / calcium-dependent protein binding / neuron projection development / presynapse / positive regulation of peptidyl-serine phosphorylation / presynaptic membrane / nuclear membrane / angiogenesis / vesicle / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of ERK1 and ERK2 cascade / negative regulation of gene expression / signaling receptor binding / neuronal cell body / glutamatergic synapse / positive regulation of gene expression / cell surface / endoplasmic reticulum / signal transduction / extracellular space / metal ion binding / plasma membrane 類似検索 - 分子機能 | |||||||||
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手法 | ![]() ![]() ![]() | |||||||||
![]() | Yamagata, A. / Fukai, S. | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction. 著者: Yamagata, A. / Goto-Ito, S. / Sato, Y. / Shiroshima, T. / Maeda, A. / Watanabe, M. / Saitoh, T. / Maenaka, K. / Terada, T. / Yoshida, T. / Uemura, T. / Fukai, S. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 820.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 684.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.6 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.6 MB | 表示 | |
XML形式データ | ![]() | 43.6 KB | 表示 | |
CIF形式データ | ![]() | 63.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
-タンパク質 , 2種, 8分子 ACEGBDFH
#1: タンパク質 | 分子量: 39723.441 Da / 分子数: 4 / 変異: H355A / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: タンパク質 | 分子量: 20953.354 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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-糖 , 2種, 16分子 ![](data/chem/img/NAG.gif)
#3: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose #4: 糖 | ChemComp-NAG / |
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-非ポリマー , 3種, 10分子 ![](data/chem/img/ZN.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#5: 化合物 | #6: 化合物 | ChemComp-CA / #7: 水 | ChemComp-HOH / | |
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-詳細
配列の詳細 | Sequence of Neurexin-1-beta was based on isoform 3b of database P58400 (NRX1B_HUMAN). |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 4 Å3/Da / 溶媒含有率: 69.26 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.8 / 詳細: 15% PEG 3350, 0.1 M sodium/potassium phosphate |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2017年5月12日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 3.4→50 Å / Num. obs: 49781 / % possible obs: 96.3 % / 冗長度: 3 % / Rsym value: 0.105 / Net I/σ(I): 7.2 |
反射 シェル | 解像度: 3.4→3.46 Å / 冗長度: 2.5 % / CC1/2: 0.526 / Rsym value: 0.427 / % possible all: 93.1 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 5Z8X,3B3Q 解像度: 3.4→49.815 Å / SU ML: 0.44 / 交差検証法: FREE R-VALUE / σ(F): 1.99 / 位相誤差: 24.64 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 3.4→49.815 Å
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拘束条件 |
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LS精密化 シェル |
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精密化 TLS | 手法: refined / Origin x: -21.4377 Å / Origin y: 23.7672 Å / Origin z: -3.8036 Å
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精密化 TLSグループ | Selection details: all |