+Open data
-Basic information
Entry | Database: PDB / ID: 5y70 | ||||||
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Title | NMR structure of KMP11 in DPC micelle | ||||||
Components | Kinetoplastid membrane protein 11 | ||||||
Keywords | MEMBRANE PROTEIN / DPC micelle / kinetoplastid membrane protein-11 / trypanosomes | ||||||
Function / homology | Function and homology information ciliary basal body organization / cell tip / axoneme / cytoskeleton organization / ciliary basal body / defense response / cilium / microtubule cytoskeleton / microtubule / cytoskeleton ...ciliary basal body organization / cell tip / axoneme / cytoskeleton organization / ciliary basal body / defense response / cilium / microtubule cytoskeleton / microtubule / cytoskeleton / positive regulation of cell population proliferation / protein-containing complex binding / protein-containing complex Similarity search - Function | ||||||
Biological species | Trypanosoma brucei brucei (eukaryote) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Lu, Y. / Lim, L.Z. / Song, J. | ||||||
Funding support | Singapore, 1items
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Citation | Journal: FEBS Lett. / Year: 2017 Title: Kinetoplastid membrane protein-11 adopts a four-helix bundle fold in DPC micelle. Authors: Lim, L.Z. / Ee, S. / Fu, J. / Tan, Y. / He, C.Y. / Song, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5y70.cif.gz | 512.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5y70.ent.gz | 439.9 KB | Display | PDB format |
PDBx/mmJSON format | 5y70.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y7/5y70 ftp://data.pdbj.org/pub/pdb/validation_reports/y7/5y70 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12071.572 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: KMP-11/1, KMP-11/2, KMP-11/3, KMP-11/4 / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): K-12 / References: UniProt: P69300 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 500 uM [U-99% 13C; U-99% 15N] KMP-11, 150 mM DPC, 90% H2O/10% D2O Details: KMP-11 in DPC / Label: sample1 / Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 200 mM / Ionic strength err: 10 / Label: condition1 / pH: 6.5 / PH err: 0.1 / Pressure: 1 Pa / Pressure err: 0.01 / Temperature: 313 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 3 | ||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 15 |