Authors state that the long gap between the residues is due to the post-translational auto-cleavage ...Authors state that the long gap between the residues is due to the post-translational auto-cleavage of the nascent polypeptide between Asn266 and Thr267.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.21 Å3/Da / 溶媒含有率: 44.47 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 1.2-2.2 M ammonium sulphate, 0.2 M NaCl, 0.1 M HEPES buffer pH 7.5-8.5
解像度: 1.2→45.08 Å / Cor.coef. Fo:Fc: 0.984 / Cor.coef. Fo:Fc free: 0.979 / SU B: 0.831 / SU ML: 0.017 / 交差検証法: THROUGHOUT / ESU R: 0.032 / ESU R Free: 0.032 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS