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Yorodumi- PDB-5xwc: Crystal Structure of Aspergillus niger Glutamate Dehydrogenase Co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5xwc | ||||||
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Title | Crystal Structure of Aspergillus niger Glutamate Dehydrogenase Complexed With Alpha-iminoglutarate, 2-amino-2-hydroxyglutarate and NADP | ||||||
Components | Glutamate dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / Glutamate Dehydrogenase / Aspergillus niger / Alpha-iminoglutarate / 2-amino-2-hydroxyglutarate / NADP / Coenzyme / Reaction Intermediate | ||||||
Function / homology | Function and homology information glutamate dehydrogenase (NADP+) activity / amino acid metabolic process / nucleotide binding Similarity search - Function | ||||||
Biological species | Aspergillus niger (mold) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å | ||||||
Authors | Prakash, P. / Punekar, N.S. / Bhaumik, P. | ||||||
Funding support | India, 1items
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Citation | Journal: J. Biol. Chem. / Year: 2018 Title: Structural basis for the catalytic mechanism and alpha-ketoglutarate cooperativity of glutamate dehydrogenase. Authors: Prakash, P. / Punekar, N.S. / Bhaumik, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5xwc.cif.gz | 131 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5xwc.ent.gz | 99.3 KB | Display | PDB format |
PDBx/mmJSON format | 5xwc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xw/5xwc ftp://data.pdbj.org/pub/pdb/validation_reports/xw/5xwc | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 49442.508 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aspergillus niger (mold) Production host: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T (others) References: UniProt: B6V7E4 |
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-Non-polymers , 6 types, 580 molecules
#2: Chemical | ChemComp-NAP / | ||||
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#3: Chemical | ChemComp-8GL / ( | ||||
#4: Chemical | ChemComp-2IT / ( | ||||
#5: Chemical | ChemComp-GOL / #6: Chemical | ChemComp-PEG / | #7: Water | ChemComp-HOH / | |
-Details
Nonpolymer details | The author states that in this structure, the alpha-imino group of alpha-iminoglutarate is ...The author states that in this structure, the alpha-imino group of alpha-iminoglutarate is protonated. As this ligand is the reaction intermediate and the alpha-imino group forms an ionic interaction with the negatively charged carboxylate group of an active site asparate, it is highly possible that this group of the ligand is in the protonated form. The existing ligand (2IT) represents the neutral alpha-amino group, therefore does not satisfy the correct ionization state of the ligand present in this structure. |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 40% v/v PEG 300, 0.1M sodium cacodylate, pH 6.5, 0.2 M calcium acetate hydrate |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 0.9537 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 1, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→35 Å / Num. obs: 138857 / % possible obs: 99 % / Redundancy: 7.9 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 13.4 |
Reflection shell | Resolution: 1.75→1.85 Å / Redundancy: 7.9 % / Mean I/σ(I) obs: 1.9 / CC1/2: 0.69 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.75→34 Å / Cor.coef. Fo:Fc: 0.975 / Cor.coef. Fo:Fc free: 0.971 / SU B: 1.431 / SU ML: 0.042 / Cross valid method: THROUGHOUT / ESU R: 0.055 / ESU R Free: 0.056 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.291 Å2
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Refinement step | Cycle: 1 / Resolution: 1.75→34 Å
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Refine LS restraints |
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