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- PDB-5xf8: Cryo-EM structure of the Cdt1-MCM2-7 complex in AMPPNP state -

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Basic information

Entry
Database: PDB / ID: 5xf8
TitleCryo-EM structure of the Cdt1-MCM2-7 complex in AMPPNP state
DescriptorDNA replication licensing factor MCM2
DNA replication licensing factor MCM3
DNA replication licensing factor MCM4
Minichromosome maintenance protein 5
DNA replication licensing factor MCM6
DNA replication licensing factor MCM7
(Cell division cycle protein ...) x 1
KeywordsHYDROLASE / helicase / DNA replication
Specimen sourceSaccharomyces cerevisiae (strain atcc 204508 / s288c) / yeast / Baker's yeast /
MethodElectron microscopy (7.1 A resolution / Single particle)
AuthorsZhai, Y. / Cheng, E. / Wu, H. / Li, N. / Yung, P.Y. / Gao, N. / Tye, B.K.
CitationNat. Struct. Mol. Biol., 2017, 24, 300-308

Nat. Struct. Mol. Biol., 2017, 24, 300-308 StrPapers
Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer.
Yuanliang Zhai / Erchao Cheng / Hao Wu / Ningning Li / Philip Yuk Kwong Yung / Ning Gao / Bik-Kwoon Tye

DateDeposition: Apr 9, 2017 / Release: May 3, 2017

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Assembly

Deposited unit
2: DNA replication licensing factor MCM2
3: DNA replication licensing factor MCM3
4: DNA replication licensing factor MCM4
5: Minichromosome maintenance protein 5
6: DNA replication licensing factor MCM6
7: DNA replication licensing factor MCM7
C: Cell division cycle protein CDT1


Theoretical massNumber of molelcules
Total (without water)677,9767
Polyers677,9767
Non-polymers00
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Polypeptide(L) , 6 types, 6 molecules 234567

#1: Polypeptide(L)DNA replication licensing factor MCM2 / Minichromosome maintenance protein 2


Mass: 98911.539 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P29469, EC: 3.6.4.12
#2: Polypeptide(L)DNA replication licensing factor MCM3 / Minichromosome maintenance protein 3


Mass: 110774.703 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P24279, EC: 3.6.4.12
#3: Polypeptide(L)DNA replication licensing factor MCM4 / Cell division control protein 54


Mass: 105138.375 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P30665, EC: 3.6.4.12
#4: Polypeptide(L)Minichromosome maintenance protein 5 / Cell division control protein 46


Mass: 86505.734 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P29496, EC: 3.6.4.12
#5: Polypeptide(L)DNA replication licensing factor MCM6 / Minichromosome maintenance protein 6


Mass: 113110.211 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P53091, EC: 3.6.4.12
#6: Polypeptide(L)DNA replication licensing factor MCM7 / Cell division control protein 47 / Minichromosome maintenance protein 7


Mass: 95049.875 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P38132, EC: 3.6.4.12

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Cell division cycle protein ... , 1 types, 1 molecules C

#7: Polypeptide(L)Cell division cycle protein CDT1 / SIC1 indispensable protein 2 / Topoisomerase-A hypersensitive protein 11


Mass: 68486.055 Da / Num. of mol.: 1
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
References: UniProt: P47112

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: Cdt1-Mcm2-7 / Type: COMPLEX
Specimen supportGrid material: COPPER
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD

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Processing

3D reconstructionResolution: 7.1 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 63000

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