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Open data
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Basic information
Entry | Database: PDB / ID: 5xeq | |||||||||
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Title | Crystal Structure of human MDGA1 and human neuroligin-2 complex | |||||||||
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![]() | CELL ADHESION / Immunogloubulin-like domain | |||||||||
Function / homology | ![]() neurotransmitter-gated ion channel clustering / jump response / positive regulation of t-SNARE clustering / symmetric, GABA-ergic, inhibitory synapse / cerebral cortex radially oriented cell migration / spinal cord association neuron differentiation / gephyrin clustering involved in postsynaptic density assembly / terminal button organization / postsynaptic density protein 95 clustering / cell-cell junction maintenance ...neurotransmitter-gated ion channel clustering / jump response / positive regulation of t-SNARE clustering / symmetric, GABA-ergic, inhibitory synapse / cerebral cortex radially oriented cell migration / spinal cord association neuron differentiation / gephyrin clustering involved in postsynaptic density assembly / terminal button organization / postsynaptic density protein 95 clustering / cell-cell junction maintenance / postsynaptic membrane assembly / positive regulation of synaptic vesicle clustering / presynaptic membrane assembly / thigmotaxis / axon guidance receptor activity / ribbon synapse / Post-translational modification: synthesis of GPI-anchored proteins / inhibitory synapse / regulation of respiratory gaseous exchange by nervous system process / insulin metabolic process / neuron cell-cell adhesion / presynapse assembly / neurexin family protein binding / dopaminergic synapse / protein localization to synapse / inhibitory synapse assembly / regulation of AMPA receptor activity / positive regulation of inhibitory postsynaptic potential / glycinergic synapse / synaptic transmission, GABAergic / protein localization to cell surface / postsynaptic specialization membrane / Neurexins and neuroligins / positive regulation of synapse assembly / regulation of synaptic membrane adhesion / positive regulation of protein localization to synapse / positive regulation of dendritic spine development / locomotory exploration behavior / homophilic cell adhesion via plasma membrane adhesion molecules / positive regulation of excitatory postsynaptic potential / social behavior / neuromuscular process controlling balance / regulation of presynapse assembly / excitatory synapse / synaptic vesicle endocytosis / GABA-ergic synapse / side of membrane / sensory perception of pain / cell adhesion molecule binding / synapse assembly / positive regulation of synaptic transmission, glutamatergic / dendritic shaft / positive regulation of synaptic transmission, GABAergic / synapse organization / neuron migration / modulation of chemical synaptic transmission / positive regulation of insulin secretion / brain development / cell-cell adhesion / presynaptic membrane / nervous system development / signaling receptor activity / chemical synaptic transmission / postsynaptic membrane / axon / neuronal cell body / synapse / positive regulation of cell population proliferation / Golgi apparatus / cell surface / extracellular space / extracellular region / identical protein binding / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kim, H.M. / Kim, J.A. / Kim, D. | |||||||||
![]() | ![]() Title: Structural Insights into Modulation of Neurexin-Neuroligin Trans-synaptic Adhesion by MDGA1/Neuroligin-2 Complex Authors: Kim, J.A. / Kim, D. / Won, S.Y. / Han, K.A. / Park, D. / Cho, E. / Yun, N. / An, H.J. / Um, J.W. / Kim, E. / Lee, J.O. / Ko, J. / Kim, H.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 176.9 KB | Display | ![]() |
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PDB format | ![]() | 136 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 736.9 KB | Display | ![]() |
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Full document | ![]() | 750.6 KB | Display | |
Data in XML | ![]() | 30.6 KB | Display | |
Data in CIF | ![]() | 40.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3bl8S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 64101.059 Da / Num. of mol.: 1 / Fragment: UNP residues 42-610 / Mutation: N98Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 35038.707 Da / Num. of mol.: 1 / Fragment: UNP residues 19-330 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#4: Sugar |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.42 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 300mM Ammonium citrate tribasic, pH 7.0, 13% 1,3-Butanediol and 18%(v/v) PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() | |||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 30, 2016 | |||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.14→49.2 Å / Num. obs: 18485 / % possible obs: 100 % / Redundancy: 12.8 % / Biso Wilson estimate: 77.18 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.075 / Rpim(I) all: 0.022 / Rrim(I) all: 0.078 / Net I/σ(I): 22.9 / Num. measured all: 236647 / Scaling rejects: 171 | |||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3BL8 Resolution: 3.136→49.199 Å / SU ML: 0.48 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 31.35 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 146.18 Å2 / Biso mean: 75.0805 Å2 / Biso min: 32.37 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.136→49.199 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 13 / % reflection obs: 100 %
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