PDB-5x8l: PD-L1 in complex with atezolizumab

Basic information

• Entry: Database: PDB / ID: 5x8l
• Title: PD-L1 in complex with atezolizumab

Components

• Programmed cell death 1 ligand 1
• atezolizumab heavy chain
• atezolizumab light chain

• Keywords: IMMUNE SYSTEM / complex / antibody

Function / homology

Function:

positive regulation of tolerance induction to tumor cell / negative regulation of tumor necrosis factor superfamily cytokine production / positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process / negative regulation of CD8-positive, alpha-beta T cell activation / TRIF-dependent toll-like receptor signaling pathway / negative regulation of CD4-positive, alpha-beta T cell proliferation / STAT3 nuclear events downstream of ALK signaling / negative regulation of interleukin-10 production / negative regulation of activated T cell proliferation / positive regulation of interleukin-10 production / negative regulation of type II interferon production / PD-1 signaling / positive regulation of T cell proliferation / T cell costimulation / response to cytokine / recycling endosome membrane / actin cytoskeleton / early endosome membrane / cellular response to lipopolysaccharide / adaptive immune response / transcription coactivator activity / cell surface receptor signaling pathway / positive regulation of cell migration / immune response / external side of plasma membrane / signal transduction / extracellular exosome / nucleoplasm / plasma membrane

Domain/homology:

CD80-like, immunoglobulin C2-set / CD80-like C2-set immunoglobulin domain / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta

Component:

Programmed cell death 1 ligand 1

• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.1 Å
• Authors: Heo, Y.S. / Lee, H.T.
• Citation: Journal: Sci Rep / Year: 2017; Title: Molecular mechanism of PD-1/PD-L1 blockade via anti-PD-L1 antibodies atezolizumab and durvalumab; Authors: Lee, H.T. / Lee, J.Y. / Lim, H. / Lee, S.H. / Moon, Y.J. / Pyo, H.J. / Ryu, S.E. / Shin, W. / Heo, Y.S.

History

Deposition	Mar 3, 2017	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Aug 16, 2017	Provider: repository / Type: Initial release

Assembly

Deposited unit

A: Programmed cell death 1 ligand 1

K: atezolizumab light chain

F: atezolizumab heavy chain

B: Programmed cell death 1 ligand 1

L: atezolizumab light chain

G: atezolizumab heavy chain

C: Programmed cell death 1 ligand 1

M: atezolizumab light chain

H: atezolizumab heavy chain

D: Programmed cell death 1 ligand 1

N: atezolizumab light chain

S: atezolizumab heavy chain

E: Programmed cell death 1 ligand 1

O: atezolizumab light chain

J: atezolizumab heavy chain

Summary:

• 311 kDa, 15 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	311,223	15
Polymers	311,223	15
Non-polymers	0	0
Water	0	0

1

A: Programmed cell death 1 ligand 1

K: atezolizumab light chain

F: atezolizumab heavy chain

Summary:

• defined by author&software
• Evidence: gel filtration
• 62.2 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	62,245	3
Polymers	62,245	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	5320 Å2
ΔGint	-33 kcal/mol
Surface area	23800 Å2
Method	PISA

2

B: Programmed cell death 1 ligand 1

L: atezolizumab light chain

G: atezolizumab heavy chain

Summary:

• defined by author&software
• 62.2 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	62,245	3
Polymers	62,245	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	5260 Å2
ΔGint	-29 kcal/mol
Surface area	23710 Å2
Method	PISA

3

C: Programmed cell death 1 ligand 1

M: atezolizumab light chain

H: atezolizumab heavy chain

Summary:

• defined by author&software
• 62.2 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	62,245	3
Polymers	62,245	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	5340 Å2
ΔGint	-28 kcal/mol
Surface area	23460 Å2
Method	PISA

4

D: Programmed cell death 1 ligand 1

N: atezolizumab light chain

S: atezolizumab heavy chain

Summary:

• defined by author&software
• 62.2 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	62,245	3
Polymers	62,245	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	5120 Å2
ΔGint	-28 kcal/mol
Surface area	24210 Å2
Method	PISA

5

E: Programmed cell death 1 ligand 1

O: atezolizumab light chain

J: atezolizumab heavy chain

Summary:

• defined by author&software
• 62.2 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	62,245	3
Polymers	62,245	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	5350 Å2
ΔGint	-32 kcal/mol
Surface area	23970 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	92.197, 169.793, 206.098
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P212121

Components

#1: Protein

A B C D E
Programmed cell death 1 ligand 1 / Programmed death ligand 1 / B7 homolog 1 / B7-H1

Details:

Mass: 14224.239 Da / Num. of mol.: 5 / Fragment: UNP residues 18-134 / Mutation: Q47E
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
Production host: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
References: UniProt: Q9NZQ7

#2: Antibody

K L M N O
atezolizumab light chain

Details:

Mass: 23386.951 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)

#3: Antibody

F G H S J
atezolizumab heavy chain

Details:

Mass: 24633.447 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.59 ų/Da / Density % sol: 52.54 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.1M Sodium HEPES pH 7.5, 20% w/v PEG8000

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 5C (4A) / Wavelength: 1 Å
• Detector: Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 23, 2016
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1 Å / Relative weight: 1
• Reflection: Resolution: 3.1→30 Å / Num. obs: 57608 / % possible obs: 97 % / Redundancy: 3.4 % / Net I/σ(I): 6.9

Processing

Software

Name	Version	Classification
PHENIX	1.9_1692	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Resolution: 3.1→29.921 Å / SU ML: 0.37 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.49 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2558	2894	5.02 %
Rwork	0.2076	-	-
obs	0.2101	57608	96.95 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.1→29.921 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	20569	0	0	0	20569

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.008	21082
X-RAY DIFFRACTION	f_angle_d	1.261	28639
X-RAY DIFFRACTION	f_dihedral_angle_d	16.078	7491
X-RAY DIFFRACTION	f_chiral_restr	0.05	3209
X-RAY DIFFRACTION	f_plane_restr	0.007	3645

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3.1001-3.1508	0.3512	144	0.2824	2519	X-RAY DIFFRACTION	96
3.1508-3.2051	0.3317	139	0.2735	2547	X-RAY DIFFRACTION	96
3.2051-3.2633	0.3923	148	0.2626	2547	X-RAY DIFFRACTION	97
3.2633-3.326	0.2688	121	0.2638	2570	X-RAY DIFFRACTION	96
3.326-3.3938	0.2956	108	0.255	2593	X-RAY DIFFRACTION	96
3.3938-3.4675	0.3384	131	0.2455	2490	X-RAY DIFFRACTION	95
3.4675-3.548	0.2727	123	0.2358	2581	X-RAY DIFFRACTION	96
3.548-3.6366	0.3055	149	0.2387	2557	X-RAY DIFFRACTION	97
3.6366-3.7348	0.2716	119	0.2418	2591	X-RAY DIFFRACTION	96
3.7348-3.8444	0.2838	137	0.2304	2574	X-RAY DIFFRACTION	96
3.8444-3.9683	0.287	135	0.2234	2566	X-RAY DIFFRACTION	97
3.9683-4.1097	0.2754	145	0.2089	2599	X-RAY DIFFRACTION	97
4.1097-4.2738	0.242	137	0.1857	2562	X-RAY DIFFRACTION	97
4.2738-4.4677	0.2166	137	0.1737	2614	X-RAY DIFFRACTION	97
4.4677-4.7024	0.2177	123	0.1603	2627	X-RAY DIFFRACTION	97
4.7024-4.9958	0.1797	158	0.1636	2637	X-RAY DIFFRACTION	99
4.9958-5.3795	0.2204	140	0.1727	2687	X-RAY DIFFRACTION	99
5.3795-5.9171	0.2271	149	0.1875	2703	X-RAY DIFFRACTION	100
5.9171-6.7646	0.2303	148	0.2037	2698	X-RAY DIFFRACTION	99
6.7646-8.4903	0.2245	151	0.1965	2714	X-RAY DIFFRACTION	99
8.4903-29.922	0.2232	152	0.176	2738	X-RAY DIFFRACTION	95

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.3473	0.062	-0.4196	2.5599	0.5342	0.286	0.2982	-0.1024	0.6478	0.2742	0.1073	-0.7276	0.2728	0.5389	-0.1601	0.0734	-0.0312	0.0527	0.5731	-0.0211	0.3699	36.9602	9.6622	62.4905
2	2.4439	-1.2129	0.5144	3.4135	1.0288	1.7953	-0.2364	0.4692	0.7875	-0.7356	0.147	0.5323	-1.0369	0.6449	-0.0106	0.6823	-0.1532	-0.1079	0.4772	-0.0879	0.5628	37.2823	29.8027	62.2168
3	1.3229	-0.2212	0.0528	0.292	-0.4628	0.825	-0.2689	0.1276	-0.0761	-0.3205	0.2738	-0.205	-0.063	-0.3715	0.0667	0.404	0.0169	-0.0171	0.3775	-0.0411	0.375	28.7876	9.0836	56.2374
4	1.8903	0.5987	-0.3559	2.8797	0.5409	1.8852	-0.0419	0.1335	-0.4289	1.1729	-0.0528	0.1429	-0.0234	0.408	0.2472	0.4201	-0.0021	-0.0541	0.2738	0.0191	0.2989	24.0516	12.6114	64.8565
5	0.7187	0.2638	0.3951	0.7552	0.091	0.5823	-0.2434	-0.5053	0.2336	0.047	-0.1553	-0.0894	0.2788	0.0467	0.0938	0.4689	0.06	-0.0191	0.2737	-0.0982	0.5315	23.6329	18.9426	69.9653
6	0.9276	-0.2083	-1.1799	3.6667	-0.2236	1.7824	-0.3045	0.1067	0.2083	0.1983	-0.0213	0.1101	-0.4778	0.1301	-0.0503	0.602	0.0585	-0.1606	0.754	0.1575	0.634	16.9782	16.7983	64.8674
7	0.4042	0.0293	-0.4678	0.7404	0.0381	0.5209	0.0469	0.1878	0.0401	0.022	0.1962	0.4827	0.0016	-0.3636	-0.094	0.8199	0.2896	-0.1523	1.2498	0.1114	0.7612	21.765	29.1443	65.7108
8	3.9655	-1.1769	-2.0202	5.5267	1.923	3.5605	-0.0885	0.5969	0.4989	-0.7596	-0.0262	1.1236	-0.3944	-0.3535	0.0553	0.3611	0.058	-0.7948	0.7063	-0.0733	-2.3292	23.9513	19.1775	55.4402
9	1.7944	-0.2296	0.0405	0.4145	-0.0174	1.9476	0.1206	-0.4709	0.0639	-0.0012	-0.2015	-0.4741	-0.634	-0.1996	0.2023	0.26	-0.115	-0.0483	0.263	-0.0175	0.4523	28.0511	16.309	56.4938
10	1.4396	0.9097	-0.5133	3.8597	3.1274	3.8058	0.2764	0.0952	0.6682	-0.3872	-0.0141	1.3137	-0.9608	-0.0916	-0.0357	0.7024	0.0481	-0.1054	0.4382	-0.1242	0.3862	30.6497	31.785	66.591
11	0.6639	0.0014	0.2785	1.2146	0.4307	1.1747	-0.1495	0.0027	0.4661	0.1154	0.1562	-0.1634	-0.3006	0.2261	0.0705	0.3748	-0.1236	-0.0015	0.391	-0.0606	0.4387	33.2025	16.1431	67.4987
12	1.0431	0.451	-0.1864	0.9043	-0.3128	1.28	-0.2729	0.0624	-0.3478	-0.1553	0.6064	0.2287	0.287	-0.1442	-0.1495	0.4898	0.0322	-0.0101	0.3366	-0.0714	0.2474	14.7979	-19.8004	69.9413
13	1.0834	0.8739	-0.164	1.2096	0.2889	0.5889	-0.0366	-0.0456	-0.1443	-0.056	0.0099	0.125	0.0503	0.107	0.0433	0.1188	0.0409	-0.0125	0.2205	0.0074	0.1949	22.8039	-13.3239	69.5544
14	0.4962	-0.0555	-0.547	2.0886	-0.815	1.2118	-0.0817	-0.0288	-0.1995	0.0277	-0.3131	-0.5589	0.0355	0.2012	0.1943	0.3306	-0.0049	-0.065	0.3088	-0.028	0.4202	20.9893	-18.8768	76.0353
15	0.9171	0.1656	-0.138	1.371	-1.6331	1.7677	-0.8596	0.2587	-0.4105	1.1641	0.5113	1.1063	-0.8418	0.6718	-0.0428	-3.2552	1.9775	-1.5816	-0.5157	0.7437	-0.3707	15.154	-4.3114	67.954
16	0.6849	-0.334	0.5891	0.7968	-0.315	0.2688	0.32	-0.0618	0.1098	0.2501	-0.3525	0.0042	-0.0837	-0.2792	0.0595	0.4397	-0.1684	0.0602	0.3867	-0.0174	0.3728	3.5001	-30.8056	90.2008
17	1.7893	1.1144	1.0925	1.8403	-0.2755	2.7948	0.0577	-0.5766	0.7521	0.6327	-0.3179	0.6039	-0.0086	-0.0109	0.2743	0.4604	-0.0801	0.0851	0.4919	-0.0526	0.593	-8.7063	-22.6792	99.8168
18	2.1414	-0.0245	0.2455	2.4016	-0.2037	1.7597	0.8166	0.1654	0.153	-0.4928	-0.302	0.3484	0.5422	-0.6075	-0.2516	0.4062	-0.0871	-0.0559	0.4764	-0.02	0.3485	-2.3513	-31.1389	85.4499
19	0.8727	0.058	0.1597	1.2671	-0.0633	0.932	0.7185	-0.8534	-1.068	0.4431	-0.2703	0.3638	-0.0335	-0.2074	-0.4624	0.5783	-0.0822	-0.004	0.3967	0.0633	0.5687	-0.3731	-26.8652	88.0754
20	0.8986	-0.2899	0.1939	2.9952	0.5379	1.6871	0.0535	0.0355	0.0149	-0.3353	-0.4881	1.2575	-0.0529	-0.5901	0.327	0.4243	-0.0096	-0.0033	0.4758	-0.0331	0.6423	-14.5172	-26.8527	94.6057
21	2.1532	-0.8194	-0.0277	1.68	0.689	0.8754	0.1084	-0.3124	-0.4384	0.0583	-0.1825	0.6358	0.0671	-0.0442	0.1571	0.5017	-0.0187	0.0729	0.2845	-0.014	0.5309	-8.0145	-37.1083	92.754
22	2.3727	0.2777	0.0969	0.8465	-0.325	0.7344	-0.0028	0.3621	0.1305	0.3299	0.0745	-0.145	0.2388	0.179	-0.073	0.3987	0.0682	-0.0661	0.3458	-0.0698	0.3372	13.9656	3.1256	89.1804
23	1.3192	-0.1811	0.1121	0.4509	0.6343	1.1326	-0.0554	-0.1512	0.1124	0.3153	0.1745	-0.1307	0.0003	0.0833	-0.044	0.2266	-0.0273	-0.0085	0.2845	0.0303	0.2159	18.4788	3.3282	80.1364
24	1.2094	0.0771	-0.2658	1.0891	-0.5578	0.9123	-0.192	-0.0348	-0.0956	-0.238	0.0214	-0.2006	-0.254	0.2341	0.1956	0.3366	0.0008	0.0201	0.324	-0.0735	0.3006	16.5495	11.4776	79.6144
25	0.5842	-0.5935	-0.0573	1.5367	0.3345	0.8398	-0.1157	0.2385	0.0053	0.1571	-0.2362	0.2753	-0.4712	-0.1795	0.243	0.311	0.0521	-0.0501	0.3669	-0.0506	0.3405	9.0677	2.2431	82.4831
26	-0.1589	0.4223	-0.1463	1.1068	0.5511	0.8728	0.1192	-0.1096	-0.2677	0.3559	-0.2788	0.0393	0.0968	0.0207	0.1083	0.3945	-0.0446	-0.0124	0.338	0.069	0.2765	7.3	-11.2424	93.8123
27	0.4363	0.6076	-0.1063	1.4444	0.7714	0.84	0.2615	-0.2727	-0.0219	0.2378	-0.2594	-0.2497	-0.1561	-0.3032	0.0825	0.2973	-0.0474	-0.0688	0.5338	-0.0447	0.4381	2.6229	-18.765	95.2577
28	0.7124	-0.1266	0.0155	0.4608	0.6678	0.7821	-0.047	0.0253	-0.3079	0.3796	-0.0427	-0.3438	0.3701	0.1017	0.1479	0.7693	-0.0846	-0.0389	0.4727	-0.0219	0.3917	3.5657	-20.7299	106.2415
29	1.0581	0.0621	0.2944	0.4027	0.0467	0.7483	0.4586	0.0262	0.2233	-0.1759	-0.238	0.4799	0.2447	0.0121	-0.0742	0.5525	0.1204	0.0213	0.4146	-0.0555	0.4645	-5.6737	-16.628	5.2918
30	0.3229	0.4526	-0.2389	1.8946	0.5879	0.6474	0.0127	0.4257	-0.1278	-0.7754	0.1139	0.0432	-0.4486	-0.4844	0.0588	0.42	-0.0065	-0.1127	0.5835	-0.0019	0.3961	-14.3883	-0.6292	1.8622
31	3.4886	0.2469	1.4731	1.5852	0.8618	3.8735	0.3779	-0.5171	-0.307	0.0512	0.4188	0.3668	-0.0872	0.0188	-0.5927	0.386	-0.0166	0.0356	0.4761	-0.0677	0.4085	-3.5543	-16.6288	17.2333
32	1.3456	-0.148	0.8033	1.6386	-1.6922	4.7559	-0.3352	0.165	0.4638	0.1713	-0.2014	-0.4916	-1.3377	0.2727	0.3382	0.4555	0.0339	-0.0456	0.3037	0.0204	0.347	2.7871	-4.4101	8.9628
33	1.692	0.6984	-0.6969	2.5426	-0.7738	2.5947	0.3574	-0.3091	0.5414	0.2222	-0.5954	0.6569	-0.0755	0.7999	-0.1893	0.6481	-0.1315	0.0791	0.563	0.0723	0.6517	3.4548	3.2108	11.9378
34	1.9132	-1.5348	1.1488	2.2589	1.0769	4.3884	-0.0311	-0.2585	0.3351	0.0773	-0.2742	-0.6445	-0.9858	-0.0003	0.0237	0.4398	-0.0616	0.0321	0.352	-0.1518	0.4355	-5.2118	4.3516	11.5925
35	1.6878	-0.2549	-0.2151	1.7635	-0.0899	1.3726	-0.3706	0.1614	0.0959	0.4772	-0.1073	0.6473	-0.3927	0.0572	0.4847	0.3659	0.0457	-0.0718	0.5578	0.0288	0.3331	-7.6268	-7.0634	14.9784
36	1.3023	-0.0842	-0.5139	0.1837	0.5947	2.9589	0.2432	0.1739	0.6479	-0.3878	0.1362	-1.0419	-0.7479	0.2188	-0.2476	0.3424	0.0044	-0.0519	0.3952	-0.0395	0.3927	-3.7301	-3.1168	3.8224
37	2.3298	-1.1458	1.3392	1.9854	-1.4428	2.5482	-0.2523	-0.3798	0.0658	-0.2186	0.2307	0.2045	-0.1993	-0.803	0.1575	0.4329	0.0033	-0.0612	0.4421	-0.0989	0.4202	-6.2938	-7.1397	-0.8008
38	0.932	0.1741	0.0778	1.0848	-0.2493	1.163	-0.1035	-0.0226	-0.245	-0.083	0.1226	-0.0439	0.3009	0.1604	0.0248	0.3602	0.0189	0.0453	0.2689	-0.0202	0.2695	20.8287	-28.2214	14.3169
39	1.2714	0.009	-0.5182	1.9499	0.2441	2.0689	0.2186	0.2169	-0.0926	-0.094	-0.2657	-0.7028	0.3475	-0.4646	0.1162	0.455	-0.0426	0.071	0.2694	-0.0224	0.3596	22.0106	-26.2893	14.2869
40	0.3977	0.16	-0.2745	0.5046	0.1334	0.3811	-0.824	0.1177	0.0261	0.4058	-0.2629	-0.7476	1.2197	0.9335	0.5055	-0.1587	0.2645	-0.3552	0.9896	0.5517	1.3157	53.7708	-25.5849	10.541
41	2.2586	3.3353	0.2058	8.5882	0.2816	0.0339	0.0903	-0.7594	-0.3412	0.3449	-0.1922	-0.2695	-0.1076	0.5446	0.0994	0.2295	0.1166	-0.6502	1.0068	0.5696	1.4069	58.9543	-20.172	11.7264
42	1.9454	-0.4783	-0.2466	3.2706	0.2033	1.5914	-0.4479	-1.1937	-0.226	0.6204	-0.748	-0.0212	-0.6015	0.2353	0.7514	0.7121	0.0243	-0.0261	0.8735	0.1675	0.6213	50.9309	-25.7519	17.6643
43	0.7492	-0.0113	-0.3513	1.0322	0.2938	1.6668	-0.1886	0.5675	-0.0761	-0.144	-0.8856	-0.2574	-0.3858	-0.2053	0.7581	0.7598	0.3196	0.1516	0.5879	0.0485	0.7299	44.1811	-32.1729	7.4851
44	0.4002	0.956	0.3601	1.6027	0.5116	0.1065	0.9655	-0.5762	-0.1314	0.1671	0.0658	0.0829	-0.585	0.6001	-0.2382	0.6599	-0.211	-0.2416	1.035	0.2601	0.9555	62.5416	-13.4312	16.3567
45	0.4012	0.5049	0.2905	0.636	0.4379	0.2963	0.2999	-0.5865	0.2408	0.1148	0.5401	-0.0492	0.1489	0.0169	-0.2106	0.9804	0.0618	-0.9082	1.2725	0.4038	1.9896	65.8289	-21.4111	20.4376
46	3.0861	2.0788	1.5775	1.4257	1.0547	0.8943	-0.1781	-0.0137	0.6987	1.0313	-0.8624	-1.3773	1.1521	0.7845	0.2863	0.6402	-0.1481	-0.5911	1.1363	0.5925	1.6543	62.2727	-31.0061	18.0914
47	1.581	0.2719	0.1396	2.7733	1.1997	2.424	-0.1927	0.595	0.1944	0.1497	0.0987	0.6894	-0.0131	0.4696	0.1051	0.4697	0.0033	-0.0239	0.2536	0.0107	0.285	28.3209	-6.3402	-0.129
48	1.2779	-0.6978	0.7672	1.4886	-0.3156	0.4405	0.6778	0.1068	0.0264	-0.2831	-0.3433	0.2138	-0.1834	0.1437	-0.1451	0.3712	-0.1066	0.1145	0.3427	-0.0182	0.2935	15.9563	-9.1158	-2.3546
49	1.2152	0.8683	-0.6432	1.7971	0.0674	1.092	0.0321	-0.2573	0.1574	0.3467	-0.2	-0.0208	-0.0537	0.1095	0.1788	0.3945	-0.0098	0.0095	0.365	-0.0435	0.2512	18.9798	-5.2854	6.5153
50	0.7879	0.2257	0.0911	0.8882	-0.0168	0.144	-0.1816	-0.0051	0.1818	-0.1042	0.0293	0.106	0.0343	0.0665	0.196	0.4216	-0.0394	0.057	0.357	0.0174	0.3175	27.3299	-9.4998	6.0013
51	0.3395	0.2222	0.1287	0.1866	0.043	0.3361	-0.3772	0.543	0.0827	-0.0044	-0.1463	-0.1512	0.4224	-0.1655	0.1998	0.488	0.286	0.0294	0.8616	0.3084	1.1063	61.3004	-21.7353	2.7626
52	1.8251	0.1931	0.6763	1.1988	-0.0986	1.5255	-0.2403	-0.3081	-0.2606	0.0567	0.0776	-0.5131	0.4384	1.1587	0.1157	0.4064	0.1025	-0.051	0.594	0.0657	0.4234	50.0155	-10.9464	4.4679
53	1.1826	-0.584	-0.663	0.5935	-0.1339	2.5781	-0.0651	-0.051	-0.4643	-0.5658	-0.4008	-0.0878	-0.2688	0.3112	0.5927	0.6608	0.0471	0.077	0.4681	0.0445	0.5631	50.043	-18.4944	2.9262
54	1.5916	-1.2239	-0.9834	0.7273	0.3586	1.8177	-0.7616	1.0181	-0.9675	-0.5464	-0.0467	-1.6736	0.0806	1.2476	0.3832	0.1245	0.7796	0.5038	0.115	0.4579	1.7621	60.1929	-27.6944	-2.6541
55	0.8765	0.3444	1.1034	1.4432	-1.0611	3.0328	0.3792	-0.2055	-0.522	-0.8175	-0.6002	-0.5729	1.1346	0.3102	0.2027	0.5332	0.0295	0.1031	0.3747	0.1458	0.6533	54.4731	-13.2249	-4.7459
56	2.6826	-0.2044	-1.1713	1.6333	1.4469	2.894	-0.0717	-0.5734	-0.5456	-0.0304	0.5588	0.0923	-0.7426	0.3785	-0.3994	0.2327	-0.0195	0.0407	0.3086	0.0446	0.4534	1.2934	-20.5192	34.0299
57	1.3418	0.8289	0.6282	1.4061	-0.1451	0.4398	-0.1602	0.0417	0.1324	-0.6249	0.2597	-0.1924	0.3978	0.0549	-0.0139	0.2585	0.015	0.0502	0.4232	-0.0372	0.3232	19.0775	-24.3347	36.8266
58	2.561	-1.3437	-0.7849	3.411	0.728	0.6723	0.8999	0.6254	-0.0219	-0.5791	-1.1218	-0.0403	-0.4949	0.012	0.0658	0.3495	-0.0229	0.0228	0.4222	-0.0179	0.3148	0.7984	-7.3695	31.6327
59	2.1703	-0.3982	0.7918	1.8329	-0.3598	0.9081	-0.2414	0.2732	0.452	0.7312	0.2966	-0.6103	-0.2469	0.2538	0.0204	0.3462	0.0137	-0.0366	0.3416	0.0052	0.321	10.3134	-9.4466	45.8599
60	3.0222	0.2488	-0.8592	0.9027	-0.8962	3.9401	0.676	0.4676	0.273	0.3217	-0.0885	-0.1483	-0.904	0.2667	-0.561	0.3903	-0.0091	0.0338	0.415	0.0546	0.2515	21.0814	-12.9492	46.5149
61	1.4309	-0.3262	0.99	0.3981	-0.1838	0.6537	-0.089	0.3806	0.267	-0.1799	-0.1328	-0.1746	0.1613	0.6135	0.0679	0.3957	0.1469	0.036	0.3038	0.051	0.318	12.3459	-10.8192	33.4352
62	0.8418	-0.3592	0.1157	0.8238	-0.7877	0.8605	-0.0485	-0.3086	0.4361	-0.0236	-0.1065	-0.1506	0.3187	0.1623	0.2046	0.3577	-0.0604	0.0799	0.2452	-0.0041	0.264	11.6958	-18.0114	42.3727
63	2.6105	-1.0264	-0.4992	1.6232	-0.0784	1.632	-0.0818	0.4049	-0.3111	-0.2281	-0.2048	-0.271	0.7204	-0.1511	0.251	0.4279	-0.001	0.0834	0.2126	-0.0217	0.2581	9.3655	-24.3286	40.9897
64	1.4217	-0.039	-0.2565	1.4127	-0.9474	0.8234	0.1705	-0.0157	0.1097	-0.0519	-0.1713	0.2045	0.0239	-0.1539	-0.0551	0.1661	-0.0204	-0.0363	0.2299	-0.0388	0.1729	-21.0741	-2.428	42.7647
65	1.3654	-0.234	-0.0057	1.0738	0.5859	0.9892	-0.1889	-0.3792	-0.0326	-0.0829	0.0678	0.3762	-0.157	-0.0257	0.1714	0.2623	-0.0243	-0.0111	0.2971	0.0555	0.2737	-19.3223	-1.6225	46.7216
66	1.9404	-0.7418	-0.1471	0.9295	-0.9915	0.9614	-0.981	-0.8031	0.0507	1.0612	0.3984	0.2569	-0.4239	-0.1655	0.2587	0.7361	0.2577	-0.0941	0.6462	-0.0216	0.3293	-36.0762	14.3101	63.4783
67	0.8028	0.604	-0.5259	0.907	-0.4358	0.3904	0.533	-0.9435	0.4242	0.4931	-0.7177	-0.2733	-0.3019	0.4208	0.2728	0.8009	0.5215	-0.4029	0.9058	-0.2171	0.6423	-27.5402	20.5934	77.5261
68	2.8641	0.6234	0.8723	1.9943	0.7809	3.2645	-1.0187	0.3514	0.222	0.5017	-0.5653	-0.5694	-1.3181	0.5476	0.9379	0.6408	0.0051	-0.279	0.3681	-0.1166	0.6067	-31.9516	19.3212	60.3005
69	2.381	0.0397	0.0503	1.0509	0.2113	1.9435	-0.2673	-0.3564	0.4304	0.0059	-0.0319	0.125	-0.4313	-0.084	0.2285	0.4901	0.1082	-0.0331	0.5175	0.1564	0.579	-29.9385	15.3719	63.496
70	0.3251	0.4403	-0.3141	1.5644	-0.4107	0.5919	-0.2016	-0.1773	0.8996	0.5955	-0.1926	0.2192	-0.2687	0.4726	0.0897	1.1118	0.2756	-0.3279	0.7077	-0.2704	0.8465	-24.1119	28.1411	75.0097
71	1.542	0.7356	0.4667	1.0368	-0.0916	1.2099	-0.4544	-0.3572	0.1531	0.6464	0.7602	-0.4267	-0.1815	0.5515	-0.175	0.7383	0.1671	-0.1638	0.4546	-0.1067	0.6633	-35.2558	27.3831	66.1734
72	0.4245	-0.4057	0.097	1.4394	0.1535	1.672	-0.5447	-1.3581	0.1745	0.9351	0.1126	0.0525	-0.1145	-1.2272	0.1886	0.1494	-0.2605	0.239	0.3656	0.348	0.0836	-7.823	-7.5052	66.457
73	1.3448	0.0497	0.3899	0.7007	0.3931	0.9077	-0.0967	0.0192	-0.0919	0.1591	0.1014	-0.0977	0.176	0.0021	0.0316	0.1745	-0.0308	-0.0088	0.1521	0.0141	0.2285	-2.0895	-8.2921	56.9139
74	1.016	0.0316	-0.009	0.5775	0.7078	1.1833	0.0608	-0.2752	0.0408	0.1823	-0.1178	-0.1272	-0.0008	-0.26	0.0707	0.2882	0.0442	-0.0043	0.3673	0.0333	0.2883	-9.4846	-3.0449	61.2733
75	1.3051	-0.8064	0.6501	0.7111	-0.6259	0.7321	-0.2466	-0.2321	0.6332	0.04	0.7392	0.2284	-0.0244	-0.6406	-0.2149	0.6066	0.8081	-0.2848	1.7569	0.2514	0.1216	-33.6778	15.6268	78.7701
76	1.3473	-1.1569	-0.2487	3.7252	1.8046	1.1056	-0.197	-0.6606	-0.3457	1.1858	0.8211	-0.3222	-0.5267	-1.4528	-0.4817	0.4673	0.198	0.1043	1.0632	0.2662	0.2756	-26.1263	4.5894	78.5654
77	1.5142	-0.6782	0.7434	1.2349	0.6933	1.4508	0.0694	-0.2631	0.1465	0.8562	0.2758	0.152	-0.2144	-0.6433	-0.2003	0.4608	0.1601	-0.0535	0.6788	0.087	0.5446	-26.6757	8.8358	72.7285
78	0.9861	-0.5737	-0.0579	1.896	-0.3549	1.6363	0.2436	-1.0421	0.0079	1.5056	0.8592	0.9406	-0.887	-1.3185	-0.3841	0.131	1.2015	0.7617	1.7824	0.0241	0.454	-42.3801	12.034	78.9933
79	0.1715	0.445	-0.0871	1.5175	0.4053	1.1433	0.0921	-0.5654	-0.125	0.4183	-0.3789	0.2081	0.4216	-0.3969	-0.0262	0.6047	0.1921	-0.0657	1.5311	0.2338	0.6868	-28.8993	6.5657	85.1446
80	0.6411	0.4847	0.3052	2.4219	0.7202	0.7273	-0.0936	0.1472	-0.3127	-0.0337	0.2765	-0.4047	-0.1026	0.0479	-0.1683	0.2318	0.0334	-0.0117	0.371	0.0891	0.3866	46.0085	-2.236	37.3541
81	3.9377	0.6281	-0.5212	1.6742	-0.3902	1.5537	-0.2569	0.0414	-1.0954	-0.0806	-0.1872	-0.5376	0.7914	0.905	0.4731	0.3503	0.0574	0.1814	0.3871	0.0658	0.4948	34.6163	-16.6696	29.1903
82	1.3794	-0.2732	-0.524	1.4912	-0.3877	0.3592	-0.3504	-0.0186	-0.3312	0.7554	0.3419	-0.0722	0.7177	0.3338	0.1673	0.4029	0.0651	0.0576	0.4389	0.0955	0.5031	39.4244	-1.5499	44.7717
83	0.8305	0.5735	0.3078	1.8838	0.5217	2.448	-0.4226	0.3547	-0.07	-0.3021	0.3029	-0.1954	-0.0648	0.2517	0.2547	0.2596	0.012	0.0052	0.3355	0.0453	0.3766	32.3779	3.0863	33.4974
84	1.6093	0.2782	0.0871	1.434	0.3629	1.4886	0.2098	0.0526	-0.3883	-0.1044	0.073	0.6902	-0.1393	-0.5003	-0.2548	0.3683	0.1218	0.0826	0.7213	0.0971	0.4559	25.5953	6.7927	40.6729
85	0.8991	0.6838	0.5485	1.5232	-0.6388	1.3362	0.02	-0.0382	-0.0577	-0.1546	0.6939	0.1303	0.141	-0.6179	-0.5075	0.4365	0.0272	-0.2544	0.5859	0.0155	0.4772	22.8689	-0.0509	29.0247
86	1.5506	1.2303	0.0797	2.2808	1.4066	1.3602	0.5287	0.7788	0.231	0.44	0.7147	1.2495	-0.3378	-1.4861	-0.7028	0.5879	-0.0785	-0.1702	0.4486	0.0639	0.2885	23.6428	-6.1489	33.5299
87	1.9407	-0.4777	1.7894	0.2547	-0.296	1.8059	0.0529	-0.3826	0.5188	-0.3139	-0.0866	0.4284	0.5844	-0.7334	-0.0638	0.3048	0.0082	-0.1068	0.5272	0.066	0.4339	33.6362	-3.8097	46.2889
88	0.9559	0.4631	0.062	1.691	0.5782	2.215	0.2045	0.4206	-0.4268	0.0792	0.4086	-0.0214	-0.0441	0.2448	-0.3964	0.3879	0.0821	-0.0253	0.3594	-0.0264	0.3477	30.2863	-10.3639	30.4333
89	0.7826	-0.4478	-0.7581	1.0666	1.5302	2.0786	-0.4794	0.0977	0.7709	-0.7663	0.2285	-0.3147	0.0951	0.0892	0.078	0.4294	0.1064	-0.0583	0.2439	0.0182	0.5389	38.719	2.2285	34.2739
90	1.9479	0.791	-0.2043	2.1957	-0.7816	0.422	0.3509	0.8524	0.0219	-0.1073	-0.3661	-0.0456	0.6937	0.2158	0.1137	0.4866	0.0491	0.0546	0.4155	-0.1018	0.3279	40.7273	-6.5151	28.3297
91	0.9842	-0.1215	0.2461	1.3601	0.4101	1.386	-0.1903	0.0514	0.1999	-0.1727	0.1201	-0.0167	-0.188	0.0964	0.0685	0.2416	-0.0411	-0.0121	0.2894	-0.0156	0.256	47.9141	27.5116	43.287
92	2.1224	1.1114	0.5724	1.8932	-0.0416	1.033	-0.4127	0.1509	0.5027	-0.6548	0.1193	0.074	-0.0572	0.4088	0.2316	0.4071	-0.0196	0.005	0.4808	0.0589	0.3762	44.9126	28.3278	40.4596
93	0.5162	0.5703	0.3256	0.4496	0.0041	-0.1058	-0.6405	0.2161	1.2353	0.1019	-0.0822	-0.2501	-0.5217	-0.1675	0.4806	0.8664	-0.1513	-0.2549	0.6944	0.0614	1.2246	38.578	58.5695	29.5817
94	1.2728	-0.0464	-0.8713	1.54	0.248	0.6513	-0.1363	-0.5663	0.6535	1.3136	-0.2346	0.3122	-0.3615	0.2971	0.213	0.705	0.047	-0.3008	0.7481	0.0177	1.0153	38.3524	58.2306	35.9421
95	2.433	0.7182	1.1756	1.5953	-0.6313	1.2178	-0.005	-0.0982	0.6747	-0.4763	-0.1384	0.4034	-0.6199	0.1209	0.3526	0.8244	-0.1015	0.0449	0.4471	-0.0238	0.6673	44.0597	50.5724	32.8631
96	1.3022	0.0901	0.7411	0.4028	-0.1824	1.4399	0.2228	-0.1705	0.4031	0.1879	-0.1676	0.1252	-0.4847	-0.4182	0.143	0.5964	0.0297	-0.11	0.5594	0.1653	1.1857	24.641	63.6926	29.0304
97	0.797	-0.014	-0.5107	-0.0071	-0.0095	0.3237	-0.1874	-0.6382	0.5422	-0.5021	-0.4237	0.7555	0.0406	0.6917	0.1019	1.2586	0.4858	-0.4532	1.135	-0.6495	1.7312	32.5393	67.6107	36.4539
98	0.9829	-0.2149	-0.0534	0.7049	-0.1686	1.0542	0.2038	-0.0916	0.6376	-0.1062	-0.2474	0.3482	-0.4635	-0.0738	0.1683	1.5064	0.2373	-0.121	0.8995	-0.0226	0.9322	39.2003	69.9386	35.1801
99	1.3076	-0.516	-0.2218	3.4824	0.3741	2.1497	0.3544	0.0022	-0.3543	-1.1133	-0.0573	0.1012	-0.2188	0.1094	-0.3076	0.3975	-0.0622	-0.0806	0.4783	-0.0545	0.4543	35.1618	20.9544	21.487
100	2.0406	-0.0461	0.175	0.6498	-0.9916	1.6902	-0.2165	0.0377	0.1142	-0.1	0.0153	0.1283	-0.1173	-0.2087	0.1394	0.2965	0.0056	0.0085	0.3004	0.042	0.3902	30.462	18.3657	29.4469
101	-0.2695	-0.547	0.0226	1.3098	-0.4935	0.5758	0.0414	0.2244	0.1563	-0.0414	-0.1436	0.0854	-0.1092	-0.1379	0.0711	0.4054	-0.021	-0.0727	0.4534	0.0289	0.4007	32.4269	25.963	26.4464
102	2.785	-0.005	-0.3506	1.36	-0.7104	1.2627	0.1651	-0.0228	0.2486	0.1801	-0.2684	0.018	-0.6149	0.9314	-0.0819	0.5323	-0.2114	-0.0939	0.787	0.2066	0.9041	38.2954	60.6673	21.2386
103	1.4308	0.169	-0.028	1.1375	0.055	1.023	0.0553	0.5032	0.5927	0.1169	0.1074	-0.2476	-0.3233	-0.0117	-0.0238	0.4499	0.0312	-0.0577	0.4913	0.101	0.9152	35.4623	47.586	18.0888
104	1.9303	0.4888	1.1022	1.9813	0.284	0.5924	0.2296	0.4696	0.1124	0.18	-0.1271	-0.2615	-0.0778	-0.1326	-0.0263	0.4525	-0.0685	-0.0797	0.5383	0.1371	0.7526	35.5705	50.1996	25.0511
105	1.1883	1.2343	-0.5617	1.3155	-0.479	0.7403	0.2043	1.3877	0.2296	-0.5307	0.1887	0.0697	-0.1054	0.6838	-0.1943	0.8389	-0.1824	0.0356	1.0178	0.2353	1.4239	45.7638	61.7401	17.2101
106	1.8571	-0.4028	0.2081	0.2713	0.5253	1.7411	-0.2797	0.1708	-0.051	-0.5149	-0.246	-0.1297	-0.3515	0.0479	0.3313	0.5374	-0.0503	-0.09	0.7384	0.1866	0.7012	34.9697	51.9017	12.2663
107	4.2178	3.5415	3.1816	4.6549	2.8866	3.573	-0.0474	0.0649	0.1395	-0.2015	-0.2575	0.8851	-0.2443	-0.2094	0.121	0.4024	0.06	-0.1017	0.5214	-0.0826	0.3585	-15.8217	29.3284	17.4998
108	0.3214	0.3133	-0.5537	0.7398	-0.2508	0.4609	0.1899	0.3219	0.2781	0.603	-0.0504	0.3376	-0.4772	0.2085	0.0432	0.5262	0.1077	-0.2152	0.4468	0.0331	0.4979	-20.6338	15.2567	12.7394
109	4.2323	-0.7738	0.79	1.242	1.1367	1.6152	0.1365	-0.1777	0.3853	0.0638	0.0505	-0.332	-0.0807	0.3349	-0.1695	0.4169	0.064	-0.0763	0.3515	-0.0793	0.334	-5.6314	23.6294	20.9935
110	2.0896	0.7124	0.0969	1.9532	-0.5341	1.5244	-0.1323	-0.3734	-0.1121	-0.2335	-0.0027	-0.4796	0.2568	0.1859	0.119	0.4924	0.0474	-0.0406	0.4206	0.0136	0.4823	-9.42	11.0498	24.0545
111	1.1167	-0.1843	1.0004	3.6918	-2.2874	2.106	-0.0309	0.1833	0.3701	-0.7176	-0.5087	-0.0305	-0.1508	0.0846	0.3457	0.3308	-0.0323	-0.086	0.3807	-0.0792	0.3919	-4.226	21.3219	11.643
112	1.3406	-0.6569	-0.3949	0.3064	0.3659	1.6368	0.2335	0.2069	-0.2732	-0.1258	-0.3233	0.1304	-0.3211	0.0198	0.0204	0.4297	0.0256	-0.1121	0.3886	-0.0249	0.3312	-17.8737	10.7915	16.6109
113	2.4287	0.934	-0.1353	1.0875	0.3308	0.318	0.6264	0.4093	0.1906	-0.3014	-0.3051	0.1232	-0.5721	0.3768	-0.1978	0.4378	0.1131	-0.0271	0.3325	0.1343	0.4703	-12.4711	23.3652	23.8816
114	0.7012	-0.3857	-0.7259	1.3329	-0.0766	0.6688	-0.087	0.0951	-0.6023	-0.1341	0.2454	0.096	0.0249	-0.2756	-0.0519	0.3795	0.0913	-0.1793	0.5204	-0.1362	0.4086	-21.9478	20.0866	20.2804
115	1.0614	-0.157	-0.17	1.0838	0.1125	1.1378	-0.1188	-0.0072	-0.0809	0.0983	0.0373	-0.3668	0.0433	0.3778	0.0565	0.3657	0.007	-0.0174	0.3858	0.0065	0.3473	7.874	40.7915	37.2993
116	1.5202	-0.0836	0.6793	1.7711	-0.8183	1.3126	-0.1525	0.1556	0.1902	0.2779	-0.1783	-0.1286	-0.3237	0.0404	0.2465	0.2317	0.0284	0.0028	0.3104	0.0453	0.3756	-4.6902	43.2352	38.7359
117	2.8193	0.4256	0.3063	0.814	0.0139	0.3927	0.3103	0.1955	1.2103	-1.0942	-0.438	-0.2643	-0.0451	1.027	-0.0755	0.4313	-0.1453	0.2376	0.3885	0.0747	0.1037	5.539	44.7166	32.1581
118	0.2618	0.31	0.6297	0.8109	0.2178	1.6929	0.0409	0.0583	0.3002	0.6059	0.259	0.2813	0.097	0.3244	-0.0918	0.379	-0.0652	0.1249	0.0895	0.0123	0.5148	2.7127	38.8567	39.6864
119	-0.0796	0.1595	0.1356	-0.1668	0.1776	1.5835	0.7999	-0.6247	0.2278	1.5289	-0.3685	0.1296	-1.2007	0.2794	-0.0515	1.4747	-0.7869	-0.1379	0.4263	-0.7768	-0.2377	9.4956	51.095	60.6386
120	0.263	0.0139	0.0669	0.3905	-0.1883	0.3644	-0.1697	-0.2018	-0.1095	0.9164	0.0555	-0.4073	-0.1834	-0.0903	0.1324	1.1201	-0.1048	-0.014	0.9561	0.0522	0.5622	7.8371	35.6405	81.3421
121	2.2828	-0.2362	0.1555	1.6901	0.0565	1.9601	-0.0252	-0.3518	0.6314	-0.9391	0.1479	-0.2158	-1.1759	0.7877	0.1206	0.8008	-0.2057	-0.0617	0.719	-0.0786	0.611	15.6286	46.7766	67.5791
122	-0.0164	-0.0922	0.0576	0.2184	0.062	-0.0182	0.0397	-0.6272	0.1611	0.0574	0.1892	-0.7313	0.267	0.2081	-0.3289	1.0201	-0.0682	0.0386	1.3188	0.133	0.6217	19.6239	37.712	74.211
123	0.5968	1.4103	-0.1842	3.748	0.5137	2.464	0.6834	0.3147	0.5878	0.3423	0.2316	0.2095	-0.7604	-1.0588	-0.6633	0.9739	-0.0623	0.2012	0.5216	0.0582	0.5685	3.9672	49.0867	60.1216
124	1.4559	0.0163	-0.1398	0.8101	0.8111	0.844	-0.4401	-0.7841	-0.1437	0.6756	-0.0905	0.1491	0.1574	0.2711	0.3456	1.0176	-0.1851	-0.1053	1.1471	0.1475	0.6482	14.6802	34.0514	78.9755
125	1.2247	0.1246	-0.1902	0.5071	0.4418	0.7958	-0.1593	-0.6024	0.3408	-0.8373	0.34	-0.1397	-0.1881	0.0602	-0.2792	1.2672	-0.205	0.1198	1.3337	0.0298	0.8846	19.0611	41.564	82.4125
126	1.0049	-0.7073	1.0983	1.1327	0.1196	1.8333	-0.2982	-0.5216	-0.2051	0.5581	0.1102	-0.8736	-0.8397	0.775	0.0382	1.528	-0.6473	-0.1187	1.1436	-0.0818	0.7464	16.5502	50.1138	78.3722
127	0.6153	0.8307	-0.0421	1.0057	0.525	1.6484	0.23	0.0606	0.1253	0.1372	-0.3	0.1639	0.3088	-0.4913	0.1489	0.3621	-0.0795	0.1353	0.3666	-0.0199	0.3827	-9.1443	23.479	52.061
128	0.8348	0.3581	0.2977	0.6719	-0.1809	0.139	0.0071	-0.2558	0.0158	0.0927	-0.1246	0.2735	0.8261	-0.3259	0.2395	0.4142	0.002	0.0955	0.3421	-0.0491	0.386	-14.7258	23.9538	41.7173
129	1.2039	0.4881	-0.0152	2.1511	-0.4975	1.3099	-0.0322	-0.022	-0.0001	0.1249	-0.055	0.0657	-0.1633	-0.0391	0.0853	0.1949	0.0153	-0.0144	0.101	-0.0345	0.2267	-5.0114	22.5921	44.6457
130	0.9589	-1.6472	-0.1297	3.2394	-0.5412	0.9832	0.3536	-0.2303	0.1994	0.1208	0.0087	-0.724	-0.5516	0.5547	-0.0991	0.9834	-0.1581	0.1716	0.777	-0.1828	0.5224	2.2881	41.5123	77.9018
131	0.6653	0.0513	-0.3945	2.532	1.0281	2.407	0.7178	-0.6546	0.4485	0.9099	-0.6471	0.5003	-0.7282	-0.3132	-0.1789	0.7879	-0.0379	0.2198	0.5044	-0.1211	0.4423	-3.852	33.0191	68.8494
132	1.4956	0.3899	-0.3487	1.0858	0.034	1.6803	0.4836	-0.9163	0.5846	0.6476	-0.3246	-0.0694	-0.2408	0.268	-0.1643	0.9294	-0.1165	0.0658	0.4891	-0.0924	0.5055	2.8344	35.8074	67.9464
133	0.0029	-0.5211	-0.3215	2.06	1.7449	1.2241	-0.1374	-0.4305	0.8065	0.7762	0.0937	0.3764	-0.2444	-0.5186	-0.0181	1.4567	-0.2966	0.4694	0.683	-0.155	0.579	-4.1246	47.5517	78.5202
134	1.9029	-0.2846	-0.0874	1.2769	-0.7666	1.7831	0.5679	-0.2636	0.7838	0.0567	0.0766	0.1542	-0.1434	-0.0738	-0.7072	0.6739	-0.0353	0.2151	0.5225	-0.0793	0.681	-8.0816	30.0575	72.6002

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 18 through 26 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 27 through 35 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 36 through 49 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 50 through 59 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 60 through 67 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 68 through 78 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 79 through 84 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 85 through 91 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 92 through 101 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 102 through 109 )
11	X-RAY DIFFRACTION	11	chain 'A' and (resid 110 through 133 )
12	X-RAY DIFFRACTION	12	chain 'K' and (resid 1 through 18 )
13	X-RAY DIFFRACTION	13	chain 'K' and (resid 19 through 75 )
14	X-RAY DIFFRACTION	14	chain 'K' and (resid 76 through 90 )
15	X-RAY DIFFRACTION	15	chain 'K' and (resid 91 through 102 )
16	X-RAY DIFFRACTION	16	chain 'K' and (resid 103 through 122 )
17	X-RAY DIFFRACTION	17	chain 'K' and (resid 123 through 137 )
18	X-RAY DIFFRACTION	18	chain 'K' and (resid 138 through 150 )
19	X-RAY DIFFRACTION	19	chain 'K' and (resid 151 through 174 )
20	X-RAY DIFFRACTION	20	chain 'K' and (resid 175 through 197 )
21	X-RAY DIFFRACTION	21	chain 'K' and (resid 198 through 213 )
22	X-RAY DIFFRACTION	22	chain 'F' and (resid 1 through 17 )
23	X-RAY DIFFRACTION	23	chain 'F' and (resid 18 through 51 )
24	X-RAY DIFFRACTION	24	chain 'F' and (resid 52 through 83 )
25	X-RAY DIFFRACTION	25	chain 'F' and (resid 84 through 98 )
26	X-RAY DIFFRACTION	26	chain 'F' and (resid 99 through 162 )
27	X-RAY DIFFRACTION	27	chain 'F' and (resid 163 through 180 )
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 181 through 218 )
29	X-RAY DIFFRACTION	29	chain 'B' and (resid 18 through 26 )
30	X-RAY DIFFRACTION	30	chain 'B' and (resid 27 through 38 )
31	X-RAY DIFFRACTION	31	chain 'B' and (resid 39 through 49 )
32	X-RAY DIFFRACTION	32	chain 'B' and (resid 50 through 68 )
33	X-RAY DIFFRACTION	33	chain 'B' and (resid 69 through 81 )
34	X-RAY DIFFRACTION	34	chain 'B' and (resid 82 through 88 )
35	X-RAY DIFFRACTION	35	chain 'B' and (resid 89 through 101 )
36	X-RAY DIFFRACTION	36	chain 'B' and (resid 102 through 119 )
37	X-RAY DIFFRACTION	37	chain 'B' and (resid 120 through 133 )
38	X-RAY DIFFRACTION	38	chain 'L' and (resid 1 through 69 )
39	X-RAY DIFFRACTION	39	chain 'L' and (resid 70 through 101 )
40	X-RAY DIFFRACTION	40	chain 'L' and (resid 102 through 127 )
41	X-RAY DIFFRACTION	41	chain 'L' and (resid 128 through 137 )
42	X-RAY DIFFRACTION	42	chain 'L' and (resid 138 through 163 )
43	X-RAY DIFFRACTION	43	chain 'L' and (resid 164 through 174 )
44	X-RAY DIFFRACTION	44	chain 'L' and (resid 175 through 187 )
45	X-RAY DIFFRACTION	45	chain 'L' and (resid 188 through 197 )
46	X-RAY DIFFRACTION	46	chain 'L' and (resid 198 through 211 )
47	X-RAY DIFFRACTION	47	chain 'G' and (resid 1 through 17 )
48	X-RAY DIFFRACTION	48	chain 'G' and (resid 18 through 33 )
49	X-RAY DIFFRACTION	49	chain 'G' and (resid 34 through 83 )
50	X-RAY DIFFRACTION	50	chain 'G' and (resid 84 through 124 )
51	X-RAY DIFFRACTION	51	chain 'G' and (resid 125 through 143 )
52	X-RAY DIFFRACTION	52	chain 'G' and (resid 144 through 155 )
53	X-RAY DIFFRACTION	53	chain 'G' and (resid 156 through 182 )
54	X-RAY DIFFRACTION	54	chain 'G' and (resid 183 through 199 )
55	X-RAY DIFFRACTION	55	chain 'G' and (resid 200 through 218 )
56	X-RAY DIFFRACTION	56	chain 'C' and (resid 18 through 26 )
57	X-RAY DIFFRACTION	57	chain 'C' and (resid 27 through 38 )
58	X-RAY DIFFRACTION	58	chain 'C' and (resid 39 through 53 )
59	X-RAY DIFFRACTION	59	chain 'C' and (resid 54 through 78 )
60	X-RAY DIFFRACTION	60	chain 'C' and (resid 79 through 84 )
61	X-RAY DIFFRACTION	61	chain 'C' and (resid 85 through 101 )
62	X-RAY DIFFRACTION	62	chain 'C' and (resid 102 through 119 )
63	X-RAY DIFFRACTION	63	chain 'C' and (resid 120 through 133 )
64	X-RAY DIFFRACTION	64	chain 'M' and (resid 1 through 75 )
65	X-RAY DIFFRACTION	65	chain 'M' and (resid 76 through 102 )
66	X-RAY DIFFRACTION	66	chain 'M' and (resid 103 through 122 )
67	X-RAY DIFFRACTION	67	chain 'M' and (resid 123 through 137 )
68	X-RAY DIFFRACTION	68	chain 'M' and (resid 138 through 150 )
69	X-RAY DIFFRACTION	69	chain 'M' and (resid 151 through 174 )
70	X-RAY DIFFRACTION	70	chain 'M' and (resid 175 through 190 )
71	X-RAY DIFFRACTION	71	chain 'M' and (resid 191 through 213 )
72	X-RAY DIFFRACTION	72	chain 'H' and (resid 1 through 17 )
73	X-RAY DIFFRACTION	73	chain 'H' and (resid 18 through 83 )
74	X-RAY DIFFRACTION	74	chain 'H' and (resid 84 through 124 )
75	X-RAY DIFFRACTION	75	chain 'H' and (resid 125 through 148 )
76	X-RAY DIFFRACTION	76	chain 'H' and (resid 149 through 162 )
77	X-RAY DIFFRACTION	77	chain 'H' and (resid 163 through 182 )
78	X-RAY DIFFRACTION	78	chain 'H' and (resid 183 through 199 )
79	X-RAY DIFFRACTION	79	chain 'H' and (resid 200 through 218 )
80	X-RAY DIFFRACTION	80	chain 'D' and (resid 18 through 26 )
81	X-RAY DIFFRACTION	81	chain 'D' and (resid 27 through 35 )
82	X-RAY DIFFRACTION	82	chain 'D' and (resid 36 through 49 )
83	X-RAY DIFFRACTION	83	chain 'D' and (resid 50 through 67 )
84	X-RAY DIFFRACTION	84	chain 'D' and (resid 68 through 73 )
85	X-RAY DIFFRACTION	85	chain 'D' and (resid 74 through 81 )
86	X-RAY DIFFRACTION	86	chain 'D' and (resid 82 through 87 )
87	X-RAY DIFFRACTION	87	chain 'D' and (resid 88 through 95 )
88	X-RAY DIFFRACTION	88	chain 'D' and (resid 96 through 109 )
89	X-RAY DIFFRACTION	89	chain 'D' and (resid 110 through 119 )
90	X-RAY DIFFRACTION	90	chain 'D' and (resid 120 through 133 )
91	X-RAY DIFFRACTION	91	chain 'N' and (resid 1 through 75 )
92	X-RAY DIFFRACTION	92	chain 'N' and (resid 76 through 102 )
93	X-RAY DIFFRACTION	93	chain 'N' and (resid 103 through 129 )
94	X-RAY DIFFRACTION	94	chain 'N' and (resid 130 through 158 )
95	X-RAY DIFFRACTION	95	chain 'N' and (resid 159 through 174 )
96	X-RAY DIFFRACTION	96	chain 'N' and (resid 175 through 188 )
97	X-RAY DIFFRACTION	97	chain 'N' and (resid 189 through 198 )
98	X-RAY DIFFRACTION	98	chain 'N' and (resid 199 through 213 )
99	X-RAY DIFFRACTION	99	chain 'S' and (resid 1 through 33 )
100	X-RAY DIFFRACTION	100	chain 'S' and (resid 34 through 76 )
101	X-RAY DIFFRACTION	101	chain 'S' and (resid 77 through 124 )
102	X-RAY DIFFRACTION	102	chain 'S' and (resid 125 through 148 )
103	X-RAY DIFFRACTION	103	chain 'S' and (resid 149 through 162 )
104	X-RAY DIFFRACTION	104	chain 'S' and (resid 163 through 182 )
105	X-RAY DIFFRACTION	105	chain 'S' and (resid 183 through 199 )
106	X-RAY DIFFRACTION	106	chain 'S' and (resid 200 through 218 )
107	X-RAY DIFFRACTION	107	chain 'E' and (resid 18 through 26 )
108	X-RAY DIFFRACTION	108	chain 'E' and (resid 27 through 41 )
109	X-RAY DIFFRACTION	109	chain 'E' and (resid 42 through 59 )
110	X-RAY DIFFRACTION	110	chain 'E' and (resid 60 through 88 )
111	X-RAY DIFFRACTION	111	chain 'E' and (resid 89 through 95 )
112	X-RAY DIFFRACTION	112	chain 'E' and (resid 96 through 109 )
113	X-RAY DIFFRACTION	113	chain 'E' and (resid 110 through 119 )
114	X-RAY DIFFRACTION	114	chain 'E' and (resid 120 through 133 )
115	X-RAY DIFFRACTION	115	chain 'O' and (resid 1 through 38 )
116	X-RAY DIFFRACTION	116	chain 'O' and (resid 39 through 61 )
117	X-RAY DIFFRACTION	117	chain 'O' and (resid 62 through 75 )
118	X-RAY DIFFRACTION	118	chain 'O' and (resid 76 through 101 )
119	X-RAY DIFFRACTION	119	chain 'O' and (resid 102 through 117 )
120	X-RAY DIFFRACTION	120	chain 'O' and (resid 118 through 136 )
121	X-RAY DIFFRACTION	121	chain 'O' and (resid 137 through 150 )
122	X-RAY DIFFRACTION	122	chain 'O' and (resid 151 through 163 )
123	X-RAY DIFFRACTION	123	chain 'O' and (resid 164 through 174 )
124	X-RAY DIFFRACTION	124	chain 'O' and (resid 175 through 186 )
125	X-RAY DIFFRACTION	125	chain 'O' and (resid 187 through 197 )
126	X-RAY DIFFRACTION	126	chain 'O' and (resid 198 through 213 )
127	X-RAY DIFFRACTION	127	chain 'J' and (resid 1 through 16 )
128	X-RAY DIFFRACTION	128	chain 'J' and (resid 17 through 33 )
129	X-RAY DIFFRACTION	129	chain 'J' and (resid 34 through 124 )
130	X-RAY DIFFRACTION	130	chain 'J' and (resid 125 through 148 )
131	X-RAY DIFFRACTION	131	chain 'J' and (resid 149 through 162 )
132	X-RAY DIFFRACTION	132	chain 'J' and (resid 163 through 182 )
133	X-RAY DIFFRACTION	133	chain 'J' and (resid 183 through 200 )
134	X-RAY DIFFRACTION	134	chain 'J' and (resid 201 through 214 )