PolycombproteinEED / hEED / Embryonic ectoderm development protein / WD protein associating with integrin cytoplasmic ...hEED / Embryonic ectoderm development protein / WD protein associating with integrin cytoplasmic tails 1 / WAIT-1
Mass: 42443.324 Da / Num. of mol.: 1 / Fragment: residues 75-401 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: EED / Plasmid: pET28GST-LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-V3R / References: UniProt: O75530
#2: Protein
EB22
Mass: 13993.254 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Plasmid: pET29b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entries 2QXV, 3LF9. The amino acid sequence of PDB entry 3LF9 was modified with the CCP4 program CHAINSAW to match the EB22 target sequence.
Resolution: 2.55→45.5 Å / Cor.coef. Fo:Fc: 0.896 / Cor.coef. Fo:Fc free: 0.887 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.608 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.612 / SU Rfree Blow DPI: 0.282 / SU Rfree Cruickshank DPI: 0.285 Details: higher resolution EED structures, such as PDB entry 3K26, were used for the interpretation of poor electron density. EED backbone coordinates were restrained to a PHENIX.ROSETTA_REFINE model. ...Details: higher resolution EED structures, such as PDB entry 3K26, were used for the interpretation of poor electron density. EED backbone coordinates were restrained to a PHENIX.ROSETTA_REFINE model. Weak electron density at and around EB22 residue Leu-68 suggested a helical fold and was interpreted homologously to PDB entry 3LF9.
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