PDB-5wmf: Crystal structure of the Hexameric Ring of Epstein-Barr Virus Nuc...

ID or keywords:

Entry

Database: PDB / ID: 5wmf

Title

Crystal structure of the Hexameric Ring of Epstein-Barr Virus Nuclear Antigen-1, EBNA1

Components

Epstein-Barr nuclear antigen 1

Keywords

VIRAL PROTEIN / EBNA1 / DNA binding protein / Epstein-Barr Virus

Function / homology

Function and homology information

host cell PML body / viral latency / Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / enzyme-substrate adaptor activity / symbiont-mediated disruption of host cell PML body / regulation of DNA replication / symbiont-mediated suppression of host NF-kappaB cascade / endonuclease activity / DNA-binding transcription factor activity / positive regulation of DNA-templated transcription / DNA binding
Similarity search - Function

Biological species

Epstein-Barr virus (Epstein-Barr virus)

Method

X-RAY DIFFRACTION /

MOLECULAR REPLACEMENT / Resolution: 1.9 Å

Authors

Messick, T.E.

Funding support

United Kingdom, 1items

Citation

Journal: J. Virol. / Year: 2017
Title: Structural and Functional Basis for an EBNA1 Hexameric Ring in Epstein-Barr Virus Episome Maintenance.
Authors: Deakyne, J.S. / Malecka, K.A. / Messick, T.E. / Lieberman, P.M.

History

Deposition	Jul 28, 2017	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 9, 2017	Provider: repository / Type: Initial release
Revision 1.1	Sep 27, 2017	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.title / _citation_author.name
Revision 1.2	Oct 4, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	5wmf.cif.gz	473.8 KB	Display	PDBx/mmCIF format
PDB format	pdb5wmf.ent.gz	397.8 KB	Display	PDB format
PDBx/mmJSON format	5wmf.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Summary document	5wmf_validation.pdf.gz	469.6 KB	Display	wwPDB validaton report
Full document	5wmf_full_validation.pdf.gz	482.6 KB	Display
Data in XML	5wmf_validation.xml.gz	34.3 KB	Display
Data in CIF	5wmf_validation.cif.gz	48.3 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/wm/5wmf ftp://data.pdbj.org/pub/pdb/validation_reports/wm/5wmf	HTTPS FTP

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Related structure data

Related structure data	1vhiS S: Starting model for refinement
Similar structure data	3pki-7 4o6n-d 6km8-1 3s7i-2 4o6m-d 5tjc-1 4q6u-d 1uf9-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: Epstein-Barr nuclear antigen 1

B: Epstein-Barr nuclear antigen 1

C: Epstein-Barr nuclear antigen 1

D: Epstein-Barr nuclear antigen 1

E: Epstein-Barr nuclear antigen 1

F: Epstein-Barr nuclear antigen 1

99.7 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: cross-linking, The paper describes experiments to support a hexameric ring structure (trimer of dimers) of EBNA1. No translational matrices are needed for the to generate the hexameric structure.

Unit cell

Length a, b, c (Å)	31.280, 84.768, 84.709
Angle α, β, γ (deg.)	60.01, 87.31, 88.53
Int Tables number	1
Space group name H-M	P1

#1: Protein	Epstein-Barr nuclear antigen 1 / EBV nuclear antigen 1 Mass: 16618.188 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Epstein-Barr virus (Epstein-Barr virus) Strain: B95-8 / Gene: EBNA1, BKRF1 / Plasmid: pET SUMO EBNA1 468-619 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P03211
#2: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 344 / Source method: isolated from a natural source / Formula: H₂O

#1: Protein

Epstein-Barr nuclear antigen 1 / EBV nuclear antigen 1

Mass: 16618.188 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Epstein-Barr virus (Epstein-Barr virus)
Strain: B95-8 / Gene: EBNA1, BKRF1 / Plasmid: pET SUMO EBNA1 468-619 / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P03211

#2: Water

ChemComp-HOH / water

Mass: 18.015 Da / Num. of mol.: 344 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 1.95 Å³/Da / Density % sol: 36.89 %
Crystal grow	Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Concentrated EBNA1 was diluted 1:1 in a solution containing 50 mM morpholineethanesulfonic acid (MES) (pH 6.5) and 800 mM sodium formate. PH range: 6.0=7.0

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54178 Å
Detector	Type: RIGAKU SATURN 944+ / Detector: CCD / Date: Oct 7, 2011 / Details: VariMax optical mirrors
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.54178 Å / Relative weight: 1
Reflection	Resolution: 1.9→50 Å / Num. obs: 48887 / % possible obs: 82.4 % / Redundancy: 1.9 % / R_sym value: 0.063 / Net I/σ(I): 9.68

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Processing

Software

Name	Version	Classification
PHENIX	1.9_1692	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 1VHI

1vhi

Resolution: 1.9→29.189 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 0.02 / Phase error: 30.92

	R_factor	Num. reflection	% reflection
R_free	0.2764	1879	4.13 %
R_work	0.1983	-	-
obs	0.2015	45443	76.46 %

Solvent computation

Shrinkage radii: 0.7 Å / VDW probe radii: 0.9 Å

Refinement step

Cycle: LAST / Resolution: 1.9→29.189 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6373	0	0	344	6717

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.015	6554
X-RAY DIFFRACTION	f_angle_d	1.599	8934
X-RAY DIFFRACTION	f_dihedral_angle_d	15.022	2431
X-RAY DIFFRACTION	f_chiral_restr	0.057	1004
X-RAY DIFFRACTION	f_plane_restr	0.009	1153

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.8989-1.9502	0.3664	51	0.2823	987	X-RAY DIFFRACTION	22
1.9502-2.0076	0.334	75	0.2791	1467	X-RAY DIFFRACTION	34
2.0076-2.0724	0.3525	86	0.2527	2064	X-RAY DIFFRACTION	46
2.0724-2.1464	0.312	155	0.2389	3112	X-RAY DIFFRACTION	72
2.1464-2.2323	0.3553	162	0.2216	3685	X-RAY DIFFRACTION	82
2.2323-2.3339	0.3005	154	0.2155	3750	X-RAY DIFFRACTION	87
2.3339-2.4568	0.3176	147	0.2157	3881	X-RAY DIFFRACTION	88
2.4568-2.6107	0.2925	141	0.2075	3915	X-RAY DIFFRACTION	88
2.6107-2.8121	0.2998	168	0.2134	4028	X-RAY DIFFRACTION	92
2.8121-3.0948	0.2497	174	0.2057	4055	X-RAY DIFFRACTION	93
3.0948-3.542	0.2747	194	0.1931	4132	X-RAY DIFFRACTION	94
3.542-4.46	0.2423	193	0.1644	4258	X-RAY DIFFRACTION	97
4.46-29.1923	0.2382	179	0.1783	4230	X-RAY DIFFRACTION	97

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.0209	-0.0178	-0.0418	0.3183	0.245	0.2692	-0.0336	0.0117	0.0511	-0.0838	-0.1346	0.2443	-0.0798	-0.1137	-0.3241	0.1762	0.0284	0.0665	-0.0641	0.0428	0.3137	-3.1385	14.4081	5.8753
2	0.0901	0.0455	0.0758	0.1794	0.0767	0.0734	-0.1111	-0.06	-0.0954	0.0099	-0.0609	0.0095	0.0756	0.0021	-0.3233	0.2557	0.2181	0.4055	0.1397	-0.0139	0.1247	7.4051	-1.6638	-0.1219
3	0.1427	0.0711	0.2494	0.0411	0.1193	0.433	0.0669	-0.0836	0.0058	0.1434	0.0081	-0.0461	0.004	0.1252	0.0846	0.1301	-0.0179	0.0539	0.0696	-0.017	0.1404	-0.6479	2.9973	16.9099
4	0.0452	0.063	-0.101	0.187	-0.106	0.1811	-0.0905	0.0793	0.313	-0.2114	0.1647	0.1673	0.1437	-0.0536	-0.1113	0.1206	0.037	-0.0244	0.1007	0.0363	0.2157	-6.2911	4.6313	11.0694
5	0.1269	0.2521	-0.0854	0.5953	-0.2629	0.1459	-0.0872	-0.3345	-0.0485	0.2848	-0.0946	-0.0162	0.0054	0.1461	-0.2421	0.3325	0.0167	0.0773	0.2606	-0.035	0.0614	1.6654	0.941	37.1497
6	0.3278	0.2397	0.1128	0.2672	-0.0694	0.3005	0.2792	-0.009	0.2223	0.1255	0.147	-0.1728	0.0104	-0.0116	0.1447	0.2576	-0.0235	0.0372	0.1671	-0.0747	0.1502	-3.671	-0.7976	33.9818
7	0.0201	0.0513	0.0605	0.1295	0.1414	0.1875	0.19	-0.0494	-0.0021	0.1085	0.0896	-0.0554	0.0603	0.0407	0.0631	-0.0012	0.0088	0.0512	0.1708	-0.0026	0.1962	4.4082	1.797	11.0675
8	0.0532	-0.011	0.0145	0.005	-0.0016	0.0058	-0.0359	0.22	-0.006	0.0456	-0.1104	-0.0726	-0.1867	0.0628	-0.0326	0.1851	-0.0338	0.0056	0.1073	0.0104	0.1707	2.7934	13.0355	17.6297
9	0.2935	0.1689	-0.2153	0.7617	-0.2144	0.1702	0.0256	0.1898	0.0346	-0.3669	0.0126	0.2552	0.0274	0.0013	0.0002	0.2054	0.0605	-0.0243	0.1926	-0.026	0.2046	-2.0326	-11.1416	8.2215
10	0.0311	-0.0385	-0.039	0.1395	0.0516	0.0447	0.0228	-0.0311	-0.0553	0.169	-0.1264	0.1483	-0.0097	-0.0444	-0.0241	0.095	-0.0596	-0.0133	0.219	0.026	0.1825	-5.1045	-21.6189	35.5305
11	0.0226	-0.015	0.0034	0.0167	-0.0057	0.0042	0.0085	0.0182	-0.1102	0.0054	-0.0858	-0.0582	-0.0292	-0.0141	-0.0046	0.0375	0.0044	-0.0424	0.1432	-0.0612	0.1313	7.7679	-21.9552	32.5759
12	0.028	0.0104	0.0094	0.0591	0.0019	0.0263	0.0416	-0.0591	0.0219	0.0162	0.0275	0.0291	-0.0042	0.0485	0.0222	-0.0244	-0.0174	0.0546	0.2387	0.1424	0.2431	14.6863	-12.9333	34.0814
13	0.4191	-0.2089	0.2742	0.2382	-0.3778	0.6265	-0.1561	-0.0044	0.1688	0.13	-0.1288	-0.1514	-0.3436	0.0454	-0.1522	0.1891	-0.0338	-0.0396	0.0958	-0.0228	0.1582	4.6781	-6.9803	25.6935
14	0.0447	0.0165	0.0801	0.0741	0.0929	0.1881	-0.0379	-0.0899	-0.0074	0.1185	0.0063	0.0823	-0.0829	-0.1235	0.0911	0.1169	-0.0071	-0.0202	0.0985	-0.0089	0.0851	-3.1675	-11.942	30.2231
15	0.0014	0.0053	0.0026	0.2002	0.0438	0.0109	-0.1038	0.1121	-0.1018	-0.0348	0.0209	0.0709	-0.0116	-0.0878	-0.0526	0.0658	-0.0009	-0.0033	0.1513	0.0116	0.0921	-6.6749	-7.9703	4.574
16	0.0568	0.0178	-0.029	0.0121	0.0067	0.0419	0.0222	0.0488	-0.039	-0.0146	0.0617	-0.0696	0.1161	-0.1001	-0.0145	0.1974	-0.0138	-0.1331	0.1753	0.0653	0.1869	-10.2479	-4.5706	6.4132
17	0.0562	-0.1185	-0.0654	0.2768	0.1182	0.1864	-0.0404	0.0159	-0.0272	0.1494	0.1491	0.0597	-0.0705	-0.0039	0.1341	0.0694	-0.024	0.0335	0.0645	0.0062	0.1435	5.7906	-12.646	24.7052
18	0.1786	0.0401	0.1481	0.1073	-0.0586	0.2172	0.1558	0.1529	0.07	-0.0662	0.0021	0.0888	0.0865	0.1632	0.1011	0.0562	0.0085	0.0245	0.0493	-0.0395	0.1227	-0.4964	-21.0816	19.9716
19	0.7785	-0.1199	-0.0866	0.3078	-0.11	0.2278	-0.0041	-0.3052	0.0847	0.239	-0.1482	0.2033	0.0243	-0.0483	-0.0505	0.1593	-0.0851	0.0848	0.2265	-0.0431	0.222	4.4159	3.0431	30.2366
20	0.0483	0.0214	0.0073	0.0118	-0.0045	0.0876	-0.0505	0.2089	0.0654	-0.071	-0.1293	-0.218	0.0611	0.0913	-0.1294	0.0093	-0.0301	0.0144	0.2786	0.0282	0.1608	1.7542	-46.1447	-21.1576
21	0.0214	0.0148	0.0164	0.0117	0.012	0.0128	-0.1156	-0.0238	0.0168	0.0909	-0.0012	-0.0429	0.0363	0.0813	-0.0203	0.1936	0.0217	0.0526	0.2933	0.0254	0.1846	15.1014	-53.9636	-9.1306
22	0.201	-0.0323	0.0206	0.473	-0.2489	0.1712	-0.0076	-0.1339	-0.0599	0.2676	-0.1643	-0.0965	0.1439	-0.0093	-0.0737	0.157	0.0932	-0.062	0.1267	0.0048	0.1329	-0.1928	-42.717	-5.8912
23	0.0406	-0.0746	-0.0006	0.1808	-0.0425	0.0287	0.0132	-0.0161	0.1467	-0.1845	0.0094	0.1262	0.0969	-0.1208	0.0957	0.0625	0.01	0.0226	0.083	0.0122	0.0472	-2.778	-48.7132	-10.6776
24	0.0426	0.0548	0.0454	0.1912	0.0551	0.0675	0.0676	0.1087	0.1172	-0.089	0.0153	0.0834	0.0132	-0.0237	-0.024	0.1512	-0.01	-0.0086	0.0614	-0.0184	0.1295	-6.9818	-27.0896	5.7764
25	0.0271	-0.0046	-0.0071	0.0012	0.0008	0.0027	0.0033	0.0382	-0.1549	-0.0195	0.1329	0.127	-0.0476	-0.0422	0.0002	0.2035	0.0557	-0.0087	0.2292	-0.0315	0.146	-10.162	-32.8035	5.0452
26	0.0103	0.0126	0.0409	0.0199	0.06	0.2046	0.0451	-0.0591	0.102	0.0349	0.0536	-0.0231	0.1712	-0.1078	0.0139	0.1188	0.0184	-0.0226	0.0982	0.0198	0.1087	7.1007	-45.3048	-6.9198
27	0.102	-0.0427	-0.0225	0.2715	0.0634	0.3975	0.229	0.0276	0.1152	-0.0274	-0.0087	-0.1101	-0.1244	0.048	0.2006	0.0846	-0.0013	0.0288	0.08	0.0256	0.1273	1.4558	-35.7267	-13.8237
28	0.1587	0.1178	-0.2484	0.1263	-0.2137	0.4003	-0.2068	-0.1046	-0.0039	0.046	0.195	0.0541	0.2983	-0.1486	0.0161	0.2656	0.02	-0.0256	0.1281	-0.0179	0.2253	2.1321	-58.4846	1.3662
29	0.0914	-0.0531	-0.0352	0.0491	-0.0208	0.1107	0.0114	0.047	-0.017	0.0273	0.0334	0.0975	-0.0103	-0.2163	0.0095	0.2655	-0.0201	0.1858	0.556	0.1468	0.1247	-9.662	-42.2661	23.7453
30	0.0653	-0.0215	0.0849	0.1235	0.1156	0.296	-0.0499	0.0161	0.036	0.057	-0.0405	0.0112	-0.0882	-0.1223	-0.2187	0.243	-0.0706	-0.0544	0.2671	0.0604	0.0452	3.1036	-38.3649	24.1429
31	0.2478	-0.0542	-0.207	0.012	0.0446	0.1728	-0.1081	-0.0266	-0.0397	0.0312	-0.0108	-0.0037	-0.0271	0.0707	-0.0094	0.5029	-0.197	0.0398	0.3401	-0.0797	0.3231	7.7414	-29.9297	17.9369
32	0.0376	-0.0303	0.0057	0.064	0.0011	0.0776	0.007	-0.1045	0.0038	0.0788	-0.0043	-0.0314	-0.0479	0.0454	0.0087	0.1456	-0.0503	-0.0423	0.0981	0.0086	0.1232	1.9289	-38.2535	7.5945
33	0.4243	-0.3632	0.1127	0.3833	-0.0801	0.0543	-0.031	-0.0154	-0.12	0.0782	0.2682	0.264	-0.1329	-0.2674	0.041	0.1961	-0.0023	0.0106	0.1429	0.0133	0.1077	-6.5774	-40.535	13.1482
34	0.0169	-0.0283	-0.0095	0.0481	0.0127	0.0074	-0.0169	0.004	-0.0851	-0.0521	0.042	-0.0591	0.0364	-0.0123	0.0388	0.2323	-0.0141	-0.087	0.0178	-0.0287	0.1904	1.138	-60.1031	-2.1826
35	0.0274	0.0166	-0.0291	0.2283	-0.181	0.1577	0.077	-0.0843	-0.0475	-0.0473	-0.0511	-0.0202	0.1302	0.0211	-0.0465	0.1683	-0.1069	-0.0243	0.1352	-0.0312	0.0603	-3.017	-58.3377	-5.2265
36	0.2047	0.1322	0.2089	0.1307	0.0676	0.3628	0.1579	-0.07	0.0152	0.0999	0.0318	-0.0153	0.2043	-0.0512	0.1896	-0.0583	-0.084	-0.1218	0.0858	-0.0425	-0.0364	3.8278	-41.0699	12.2959
37	0.0259	-0.0093	-0.028	0.0104	0.0046	0.0132	-0.073	-0.1387	0.0154	0.1627	-0.0188	-0.0154	0.0999	0.1084	0.0001	0.1674	0.0045	0.003	0.1356	-0.0138	0.1227	1.5546	-51.5528	16.6467
38	0.4067	0.4064	0.332	0.4017	0.3284	0.2613	-0.0358	-0.227	0.2892	0.1008	-0.0579	0.2402	-0.1881	-0.2322	-0.0373	0.184	0.0061	0.0254	0.2434	0.0011	0.1432	-1.3774	-29.8608	0.8386
39	0.0432	0.0662	0.0415	0.1295	0.031	0.1483	-0.0854	0.0009	0.0047	-0.0746	-0.1259	0.1279	0.0295	-0.1525	-0.4483	0.2803	-0.0161	-0.1226	0.1159	-0.3303	-0.1211	-1.7824	-28.4251	-32.2267
40	0.0648	0.0064	0.0269	0.0326	-0.0164	0.0181	0.0978	0.0797	-0.0546	-0.0227	0.0073	-0.0349	0.1542	0.0719	0.1126	0.3666	0.1427	-0.2772	-0.0515	0.1907	0.3072	7.219	-25.8355	-15.6107
41	0.0181	-0.0479	-0.0228	0.1138	0.0449	0.0191	0.0856	0.1612	0.0237	-0.2111	-0.0777	0.0849	-0.0637	0.1082	-0.03	0.1918	0.0383	-0.0087	0.1443	0.0109	0.1189	-0.5075	-13.25	-27.8824
42	0.5736	-0.0425	-0.32	0.0031	0.0282	0.1785	-0.1082	0.2837	-0.3571	0.0335	-0.0086	0.0395	0.2787	-0.262	0.0267	0.2433	-0.02	-0.0747	0.0814	0.0007	0.032	-6.2703	-19.0387	-26.4678
43	0.1211	-0.0217	-0.0548	0.1199	0.0524	0.1565	0.0434	0.0336	0.1696	-0.1174	-0.0044	-0.0089	-0.2379	0.0046	0.0863	0.1706	0.0276	0.017	0.1166	0.0344	0.1386	1.4485	4.9394	-36.5636
44	0.3294	-0.1966	-0.1944	0.2867	0.0175	0.1639	0.2571	0.1372	-0.1964	-0.1476	0.0698	0.1684	-0.0291	0.1001	0.0619	0.2357	0.0117	-0.0524	0.1211	-0.0039	0.1074	-3.4695	3.367	-33.1417
45	0.0134	0.0246	-0.0408	0.064	-0.111	0.1737	0.0811	-0.0487	-0.0061	0.0788	0.1085	-0.1026	0.0824	-0.0384	0.2807	0.0159	0.133	-0.0582	0.0492	0.082	0.084	4.4583	-17.7386	-23.7933
46	0.0247	0.0203	-0.0047	0.0214	-0.0217	0.0176	0.0912	-0.0089	-0.0034	-0.1278	0.0278	0.0201	0.1141	0.0007	0.0001	0.1927	0.019	-0.0049	0.1757	-0.0021	0.0937	3.0658	-17.6139	-36.8125
47	0.3518	-0.4386	0.5849	0.8201	-0.5754	1.0537	0.0701	-0.178	-0.1823	0.1681	0.0437	0.429	0.2955	-0.3371	0.08	0.2314	-0.0124	-0.0392	0.1381	0.0125	0.1717	-2.0874	-13.4896	-11.3509
48	0.1113	0.0216	-0.0061	0.0097	-0.0065	0.0047	0.0007	-0.0541	0.0163	-0.0495	0.0411	0.0332	-0.0767	0.0259	0.0825	0.0589	0.0325	-0.0165	0.1581	-0.0727	0.2032	-5.4082	15.5201	-16.2302
49	0.0246	0.0079	0.0039	0.0612	0.0976	0.1556	0.0435	-0.0041	-0.0014	0.0156	0.1336	-0.0103	-0.0073	0.0105	0.1685	-0.0701	0.0193	-0.0128	0.2097	0.0159	0.1228	7.873	12.7257	-14.0707
50	0.0271	-0.0547	0.0735	0.1156	-0.1579	0.2104	0.1144	0.1494	0.0209	-0.0538	0.0639	0.0095	0.0145	-0.0003	0.026	0.073	-0.0173	0.0326	0.2165	0.0168	0.2048	14.8034	9.5838	-22.5352
51	0.4722	-0.1794	-0.2219	0.2135	0.031	0.5567	0.1145	0.0587	-0.1399	-0.2939	0.1933	-0.1165	0.0233	0.102	0.2226	0.2198	0.0002	0.0829	0.1427	-0.0788	0.0982	4.7933	-0.6351	-23.555
52	0.0415	-0.1028	-0.0978	0.428	0.1321	0.3104	-0.0834	0.0286	0.0466	-0.0931	-0.0755	0.1631	-0.1165	-0.1523	-0.1903	0.054	-0.004	0.0017	0.1063	-0.0152	0.0406	-3.0861	5.7422	-21.5584
53	-0.0011	0.0006	-0.0015	0.0024	0.0084	0.0398	-0.042	0.0241	-0.0322	0.0569	0.0301	0.0258	0.0743	-0.0048	0.0058	0.1731	-0.0195	0.0181	0.0948	0.0302	0.0727	-6.6636	-18.485	-12.1774
54	0.0067	-0.0262	0.0015	0.0989	0.0011	-0.0002	-0.0898	0.0572	-0.005	-0.0788	0.0512	-0.02	0.0159	-0.005	0.0026	0.2776	-0.098	-0.0189	0.0844	-0.0097	0.2073	-10.3499	-18.5562	-16.2294
55	0.0698	-0.0123	0.0046	0.0037	-0.0166	-0.0001	0.0492	0.1216	0.0035	-0.0191	0.0138	0.1174	-0.132	-0.057	0.0154	0.0816	0.0177	-0.0017	0.1535	-0.0052	0.1149	5.8638	1.3155	-18.1862
56	0.0722	-0.0085	-0.012	0.1178	-0.0007	0.0077	0.0359	-0.0734	0.1524	0.1525	-0.029	0.055	-0.0038	-0.0027	-0.0658	0.1001	-0.0371	-0.0333	0.1379	-0.0097	0.093	-0.4384	1.3868	-8.4946
57	0.2662	0.2004	0.2973	0.1458	0.2153	0.3126	-0.2599	0.2265	-0.0735	-0.2219	-0.1066	0.0469	-0.1416	-0.1792	-0.1244	0.2646	0.057	-0.0235	0.2464	0.0046	0.1605	2.8473	0.1156	-35.7865

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 475:492 )	A	475 - 492
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 493:501 )	A	493 - 501
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 502:516 )	A	502 - 516
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 517:537 )	A	517 - 537
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 538:549 )	A	538 - 549
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 550:560 )	A	550 - 560
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 561:576 )	A	561 - 576
8	X-RAY DIFFRACTION	8	( CHAIN A AND RESID 577:599 )	A	577 - 599
9	X-RAY DIFFRACTION	9	( CHAIN A AND RESID 600:611 )	A	600 - 611
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 475:483 )	B	475 - 483
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 484:491 )	B	484 - 491
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 492:497 )	B	492 - 497
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 498:514 )	B	498 - 514
14	X-RAY DIFFRACTION	14	( CHAIN B AND RESID 515:537 )	B	515 - 537
15	X-RAY DIFFRACTION	15	( CHAIN B AND RESID 538:548 )	B	538 - 548
16	X-RAY DIFFRACTION	16	( CHAIN B AND RESID 549:558 )	B	549 - 558
17	X-RAY DIFFRACTION	17	( CHAIN B AND RESID 559:582 )	B	559 - 582
18	X-RAY DIFFRACTION	18	( CHAIN B AND RESID 583:598 )	B	583 - 598
19	X-RAY DIFFRACTION	19	( CHAIN B AND RESID 599:611 )	B	599 - 611
20	X-RAY DIFFRACTION	20	( CHAIN C AND RESID 476:492 )	C	476 - 492
21	X-RAY DIFFRACTION	21	( CHAIN C AND RESID 493:501 )	C	493 - 501
22	X-RAY DIFFRACTION	22	( CHAIN C AND RESID 502:516 )	C	502 - 516
23	X-RAY DIFFRACTION	23	( CHAIN C AND RESID 517:537 )	C	517 - 537
24	X-RAY DIFFRACTION	24	( CHAIN C AND RESID 538:549 )	C	538 - 549
25	X-RAY DIFFRACTION	25	( CHAIN C AND RESID 550:560 )	C	550 - 560
26	X-RAY DIFFRACTION	26	( CHAIN C AND RESID 561:576 )	C	561 - 576
27	X-RAY DIFFRACTION	27	( CHAIN C AND RESID 577:599 )	C	577 - 599
28	X-RAY DIFFRACTION	28	( CHAIN C AND RESID 600:613 )	C	600 - 613
29	X-RAY DIFFRACTION	29	( CHAIN D AND RESID 475:483 )	D	475 - 483
30	X-RAY DIFFRACTION	30	( CHAIN D AND RESID 484:491 )	D	484 - 491
31	X-RAY DIFFRACTION	31	( CHAIN D AND RESID 492:497 )	D	492 - 497
32	X-RAY DIFFRACTION	32	( CHAIN D AND RESID 498:514 )	D	498 - 514
33	X-RAY DIFFRACTION	33	( CHAIN D AND RESID 515:537 )	D	515 - 537
34	X-RAY DIFFRACTION	34	( CHAIN D AND RESID 538:548 )	D	538 - 548
35	X-RAY DIFFRACTION	35	( CHAIN D AND RESID 549:558 )	D	549 - 558
36	X-RAY DIFFRACTION	36	( CHAIN D AND RESID 559:582 )	D	559 - 582
37	X-RAY DIFFRACTION	37	( CHAIN D AND RESID 583:598 )	D	583 - 598
38	X-RAY DIFFRACTION	38	( CHAIN D AND RESID 599:611 )	D	599 - 611
39	X-RAY DIFFRACTION	39	( CHAIN E AND RESID 477:492 )	E	477 - 492
40	X-RAY DIFFRACTION	40	( CHAIN E AND RESID 493:501 )	E	493 - 501
41	X-RAY DIFFRACTION	41	( CHAIN E AND RESID 502:516 )	E	502 - 516
42	X-RAY DIFFRACTION	42	( CHAIN E AND RESID 517:537 )	E	517 - 537
43	X-RAY DIFFRACTION	43	( CHAIN E AND RESID 538:549 )	E	538 - 549
44	X-RAY DIFFRACTION	44	( CHAIN E AND RESID 550:560 )	E	550 - 560
45	X-RAY DIFFRACTION	45	( CHAIN E AND RESID 561:576 )	E	561 - 576
46	X-RAY DIFFRACTION	46	( CHAIN E AND RESID 577:599 )	E	577 - 599
47	X-RAY DIFFRACTION	47	( CHAIN E AND RESID 600:611 )	E	600 - 611
48	X-RAY DIFFRACTION	48	( CHAIN F AND RESID 474:483 )	F	474 - 483
49	X-RAY DIFFRACTION	49	( CHAIN F AND RESID 484:491 )	F	484 - 491
50	X-RAY DIFFRACTION	50	( CHAIN F AND RESID 492:497 )	F	492 - 497
51	X-RAY DIFFRACTION	51	( CHAIN F AND RESID 498:514 )	F	498 - 514
52	X-RAY DIFFRACTION	52	( CHAIN F AND RESID 515:537 )	F	515 - 537
53	X-RAY DIFFRACTION	53	( CHAIN F AND RESID 538:548 )	F	538 - 548
54	X-RAY DIFFRACTION	54	( CHAIN F AND RESID 549:558 )	F	549 - 558
55	X-RAY DIFFRACTION	55	( CHAIN F AND RESID 559:582 )	F	559 - 582
56	X-RAY DIFFRACTION	56	( CHAIN F AND RESID 583:598 )	F	583 - 598
57	X-RAY DIFFRACTION	57	( CHAIN F AND RESID 599:613 )	F	599 - 613

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