- PDB-5wip: TraE protein in complex with 2-(2-furyl)isonicotinic acid -
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Entry
Database: PDB / ID: 5wip
Title
TraE protein in complex with 2-(2-furyl)isonicotinic acid
Components
Conjugal transfer protein
Keywords
PROTEIN TRANSPORT / Type IV Secretion System / Fragment-Based Drug Design / Complex / Inhibitor / Antimicrobial Resistance / Bacterial secretion / VirB
VirB8 protein / Type IV secretion system protein VirB8/PtlE / Bacterial virulence protein VirB8 / VirB8 protein / NTF2-like domain superfamily / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta Similarity search - Domain/homology
A: Conjugal transfer protein D: Conjugal transfer protein hetero molecules
defined by author
Evidence: assay for oligomerization, Bacterial two-hybrid assay corroborates the dimer form, cross-linking, Crosslinking experiments with DSS show formation of homomultimeric forms, gel filtration, ...Evidence: assay for oligomerization, Bacterial two-hybrid assay corroborates the dimer form, cross-linking, Crosslinking experiments with DSS show formation of homomultimeric forms, gel filtration, Analytic gel filtration corroborates the dimeric form
Conjugaltransferprotein / Conjugal transfer protein TraE / TraE / TraE protein / Inner membrane protein forms channel for ...Conjugal transfer protein TraE / TraE / TraE protein / Inner membrane protein forms channel for type IV secretion of T-DNA complex / VirB8 / Type IV secretion of T-DNA VirB8 / Type IV secretion system protein VirB8
Mass: 18818.104 Da / Num. of mol.: 4 / Fragment: residues 70-232 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) Gene: traE, B634_00049, BE957_00530, ECONIH1_27615, pec386IL_00125, pEcNDM0_00049, pHKU1_33, pKC394-028, pKC396-021, pMUR050_041, pN3_023 Plasmid: PHT / Cell line (production host): BL21(DE3)STAR / Production host: Escherichia coli (E. coli) / References: UniProt: Q17U16
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