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- PDB-5wbh: Structure of the FRB domain of mTOR bound to a substrate recruitm... -

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Basic information

Entry
Database: PDB / ID: 5wbh
TitleStructure of the FRB domain of mTOR bound to a substrate recruitment peptide of S6K1
Components
  • Ribosomal protein S6 kinase beta-1
  • Serine/threonine-protein kinase mTOR
KeywordsTRANSFERASE
Function / homology
Function and homology information


long-chain fatty acid import into cell / RNA polymerase III type 2 promoter sequence-specific DNA binding / RNA polymerase III type 1 promoter sequence-specific DNA binding / positive regulation of cytoplasmic translational initiation / regulation of locomotor rhythm / T-helper 1 cell lineage commitment / positive regulation of pentose-phosphate shunt / positive regulation of wound healing, spreading of epidermal cells / TORC2 signaling / TORC2 complex ...long-chain fatty acid import into cell / RNA polymerase III type 2 promoter sequence-specific DNA binding / RNA polymerase III type 1 promoter sequence-specific DNA binding / positive regulation of cytoplasmic translational initiation / regulation of locomotor rhythm / T-helper 1 cell lineage commitment / positive regulation of pentose-phosphate shunt / positive regulation of wound healing, spreading of epidermal cells / TORC2 signaling / TORC2 complex / regulation of membrane permeability / cellular response to leucine starvation / negative regulation of lysosome organization / heart valve morphogenesis / TFIIIC-class transcription factor complex binding / TORC1 complex / voluntary musculoskeletal movement / positive regulation of transcription of nucleolar large rRNA by RNA polymerase I / negative regulation of TORC2 signaling / calcineurin-NFAT signaling cascade / RNA polymerase III type 3 promoter sequence-specific DNA binding / positive regulation of keratinocyte migration / regulation of osteoclast differentiation / regulation of lysosome organization / MTOR signalling / cellular response to L-leucine / energy reserve metabolic process / regulation of autophagosome assembly / Energy dependent regulation of mTOR by LKB1-AMPK / cellular response to nutrient / Amino acids regulate mTORC1 / TORC1 signaling / serine/threonine protein kinase complex / ruffle organization / cellular response to methionine / negative regulation of cell size / positive regulation of ubiquitin-dependent protein catabolic process / cellular response to osmotic stress / negative regulation of protein localization to nucleus / anoikis / inositol hexakisphosphate binding / cardiac muscle cell development / negative regulation of calcineurin-NFAT signaling cascade / regulation of myelination / positive regulation of transcription by RNA polymerase III / negative regulation of macroautophagy / Macroautophagy / phosphatidylinositol-mediated signaling / positive regulation of myotube differentiation / regulation of cell size / Constitutive Signaling by AKT1 E17K in Cancer / positive regulation of actin filament polymerization / germ cell development / behavioral response to pain / TOR signaling / oligodendrocyte differentiation / positive regulation of oligodendrocyte differentiation / mTORC1-mediated signalling / positive regulation of translational initiation / CD28 dependent PI3K/Akt signaling / HSF1-dependent transactivation / regulation of macroautophagy / cellular response to dexamethasone stimulus / response to amino acid / 'de novo' pyrimidine nucleobase biosynthetic process / positive regulation of epithelial to mesenchymal transition / behavioral fear response / vascular endothelial cell response to laminar fluid shear stress / positive regulation of lipid biosynthetic process / heart morphogenesis / cellular response to nutrient levels / neuronal action potential / regulation of cellular response to heat / positive regulation of lamellipodium assembly / cardiac muscle contraction / T cell costimulation / positive regulation of stress fiber assembly / phagocytic vesicle / endomembrane system / cytoskeleton organization / protein serine/threonine/tyrosine kinase activity / positive regulation of TORC1 signaling / positive regulation of mitotic cell cycle / negative regulation of insulin receptor signaling pathway / negative regulation of autophagy / cellular response to amino acid starvation / regulation of signal transduction by p53 class mediator / positive regulation of glycolytic process / cellular response to starvation / positive regulation of translation / Regulation of PTEN gene transcription / VEGFR2 mediated vascular permeability / post-embryonic development / negative regulation of extrinsic apoptotic signaling pathway / TP53 Regulates Metabolic Genes / regulation of actin cytoskeleton organization / cellular response to amino acid stimulus / non-specific protein-tyrosine kinase / macroautophagy / regulation of cell growth
Similarity search - Function
FKBP12-rapamycin binding domain / Ribosomal protein S6 kinase / Domain of unknown function DUF3385, target of rapamycin protein / Serine/threonine-protein kinase mTOR domain / Domain of unknown function / FKBP12-rapamycin binding domain / Serine/threonine-protein kinase TOR / FKBP12-rapamycin binding domain superfamily / FKBP12-rapamycin binding domain / Rapamycin binding domain ...FKBP12-rapamycin binding domain / Ribosomal protein S6 kinase / Domain of unknown function DUF3385, target of rapamycin protein / Serine/threonine-protein kinase mTOR domain / Domain of unknown function / FKBP12-rapamycin binding domain / Serine/threonine-protein kinase TOR / FKBP12-rapamycin binding domain superfamily / FKBP12-rapamycin binding domain / Rapamycin binding domain / Serine/threonine-protein kinase ATR-like, HEAT repeats / Protein kinase, C-terminal / Protein kinase C terminal domain / : / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Extension to Ser/Thr-type protein kinases / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / AGC-kinase, C-terminal / AGC-kinase C-terminal domain profile. / Four Helix Bundle (Hemerythrin (Met), subunit A) / Armadillo-like helical / Tetratricopeptide-like helical domain superfamily / Armadillo-type fold / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Ribosomal protein S6 kinase beta-1 / Serine/threonine-protein kinase mTOR
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å
AuthorsPavletich, N.P. / Yang, H.
CitationJournal: Nature / Year: 2017
Title: Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Authors: Haijuan Yang / Xiaolu Jiang / Buren Li / Hyo J Yang / Meredith Miller / Angela Yang / Ankita Dhar / Nikola P Pavletich /
Abstract: The mechanistic target of rapamycin complex 1 (mTORC1) controls cell growth and metabolism in response to nutrients, energy levels, and growth factors. It contains the atypical kinase mTOR and the ...The mechanistic target of rapamycin complex 1 (mTORC1) controls cell growth and metabolism in response to nutrients, energy levels, and growth factors. It contains the atypical kinase mTOR and the RAPTOR subunit that binds to the Tor signalling sequence (TOS) motif of substrates and regulators. mTORC1 is activated by the small GTPase RHEB (Ras homologue enriched in brain) and inhibited by PRAS40. Here we present the 3.0 ångström cryo-electron microscopy structure of mTORC1 and the 3.4 ångström structure of activated RHEB-mTORC1. RHEB binds to mTOR distally from the kinase active site, yet causes a global conformational change that allosterically realigns active-site residues, accelerating catalysis. Cancer-associated hyperactivating mutations map to structural elements that maintain the inactive state, and we provide biochemical evidence that they mimic RHEB relieving auto-inhibition. We also present crystal structures of RAPTOR-TOS motif complexes that define the determinants of TOS recognition, of an mTOR FKBP12-rapamycin-binding (FRB) domain-substrate complex that establishes a second substrate-recruitment mechanism, and of a truncated mTOR-PRAS40 complex that reveals PRAS40 inhibits both substrate-recruitment sites. These findings help explain how mTORC1 selects its substrates, how its kinase activity is controlled, and how it is activated by cancer-associated mutations.
History
DepositionJun 29, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 20, 2017Provider: repository / Type: Initial release
Revision 1.1Apr 18, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Serine/threonine-protein kinase mTOR
B: Serine/threonine-protein kinase mTOR
C: Serine/threonine-protein kinase mTOR
D: Serine/threonine-protein kinase mTOR
E: Serine/threonine-protein kinase mTOR
W: Ribosomal protein S6 kinase beta-1


Theoretical massNumber of molelcules
Total (without water)63,5166
Polymers63,5166
Non-polymers00
Water6,017334
1
A: Serine/threonine-protein kinase mTOR


Theoretical massNumber of molelcules
Total (without water)12,0921
Polymers12,0921
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area6100 Å2
MethodPISA
2
B: Serine/threonine-protein kinase mTOR


Theoretical massNumber of molelcules
Total (without water)12,0921
Polymers12,0921
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area5770 Å2
MethodPISA
3
C: Serine/threonine-protein kinase mTOR


Theoretical massNumber of molelcules
Total (without water)12,0921
Polymers12,0921
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area6360 Å2
MethodPISA
4
D: Serine/threonine-protein kinase mTOR
W: Ribosomal protein S6 kinase beta-1


Theoretical massNumber of molelcules
Total (without water)15,1492
Polymers15,1492
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area880 Å2
ΔGint-8 kcal/mol
Surface area7320 Å2
MethodPISA
5
E: Serine/threonine-protein kinase mTOR


Theoretical massNumber of molelcules
Total (without water)12,0921
Polymers12,0921
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area6450 Å2
MethodPISA
Unit cell
Length a, b, c (Å)60.613, 80.944, 134.848
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP22121

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Components

#1: Protein
Serine/threonine-protein kinase mTOR / FK506-binding protein 12-rapamycin complex-associated protein 1 / FKBP12-rapamycin complex- ...FK506-binding protein 12-rapamycin complex-associated protein 1 / FKBP12-rapamycin complex-associated protein / Mammalian target of rapamycin / mTOR / Mechanistic target of rapamycin / Rapamycin and FKBP12 target 1 / Rapamycin target protein 1


Mass: 12091.749 Da / Num. of mol.: 5 / Fragment: residues 2018-2114
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1 / Plasmid: pET26 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: P42345, non-specific serine/threonine protein kinase
#2: Protein/peptide Ribosomal protein S6 kinase beta-1 / S6K1 / 70 kDa ribosomal protein S6 kinase 1 / p70-S6K 1 / Ribosomal protein S6 kinase I / ...S6K1 / 70 kDa ribosomal protein S6 kinase 1 / p70-S6K 1 / Ribosomal protein S6 kinase I / Serine/threonine-protein kinase 14A / p70 ribosomal S6 kinase alpha / p70 S6KA


Mass: 3057.544 Da / Num. of mol.: 1 / Fragment: residues 412-437
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RPS6KB1, STK14A / Plasmid: pET26 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: P23443, non-specific serine/threonine protein kinase
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 334 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.77 Å3/Da / Density % sol: 55.58 % / Mosaicity: 0.738 °
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: tacsimate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 12, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 1.75→50 Å / Num. obs: 66487 / % possible obs: 98.2 % / Redundancy: 4.9 % / Rmerge(I) obs: 0.068 / Rpim(I) all: 0.032 / Rrim(I) all: 0.076 / Χ2: 1.575 / Net I/σ(I): 11.6 / Num. measured all: 328879
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
1.75-1.8140.603615361530.7270.3040.680.89292.4
1.81-1.894.50.48964840.8460.240.5470.96297.1
1.89-1.974.80.39166280.9110.190.4361.16898.8
1.97-2.075.30.27766790.950.1290.3071.2299.8
2.07-2.25.20.18166990.9780.0850.21.30799.8
2.2-2.384.80.13266510.9850.0650.1481.59898.9
2.38-2.615.40.09867550.9930.0450.1081.60699.8
2.61-2.9950.07267090.9960.0340.081.91599.1
2.99-3.775.30.05367900.9970.0240.0582.46698.9
3.77-5050.0469390.9980.0190.0452.21497.3

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
REFMAC5.8.0158refinement
PDB_EXTRACT3.22data extraction
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1FAP

1fap


Resolution: 1.75→50.01 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.955 / SU B: 7.489 / SU ML: 0.102 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.111 / ESU R Free: 0.102
Details: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
RfactorNum. reflection% reflectionSelection details
Rfree0.2102 1932 3 %RANDOM
Rwork0.1931 ---
obs0.1937 61680 93.93 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å
Displacement parametersBiso max: 130.28 Å2 / Biso mean: 37.909 Å2 / Biso min: 12.88 Å2
Baniso -1Baniso -2Baniso -3
1--3.8 Å2-0 Å20 Å2
2--5.45 Å2-0 Å2
3----1.65 Å2
Refinement stepCycle: final / Resolution: 1.75→50.01 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4108 0 0 334 4442
Biso mean---46.87 -
Num. residues----485
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0070.0194221
X-RAY DIFFRACTIONr_bond_other_d0.0010.023936
X-RAY DIFFRACTIONr_angle_refined_deg1.0661.9275667
X-RAY DIFFRACTIONr_angle_other_deg0.8923.0018963
X-RAY DIFFRACTIONr_dihedral_angle_1_deg4.9515479
X-RAY DIFFRACTIONr_dihedral_angle_2_deg32.31523.203231
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.09815775
X-RAY DIFFRACTIONr_dihedral_angle_4_deg13.4591535
X-RAY DIFFRACTIONr_chiral_restr0.0650.2546
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.024722
X-RAY DIFFRACTIONr_gen_planes_other0.0010.021069
LS refinement shellResolution: 1.75→1.795 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.353 119 -
Rwork0.36 3649 -
all-3768 -
obs--76.04 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.1130.68472.30891.20040.46224.0271-0.0189-0.211-0.03020.06910.057-0.0650.0685-0.0158-0.0380.26340.02320.0230.1826-0.01540.2078-12.42721.397-8.094
25.8582-0.7562-1.4490.66140.14662.2255-0.096-0.0385-0.1363-0.05170.0513-0.04560.06920.04950.04470.28970.0062-0.00620.14260.01690.183717.68321.7311.004
35.56670.26611.74761.53260.27831.407-0.0198-0.19180.3220.1001-0.01170.0533-0.1230.03620.03150.31330.01340.01530.0207-0.0140.2910.278-0.195-30.097
42.7379-0.1032-1.9592.0190.40814.29840.01070.10640.0413-0.0372-0.0136-0.08460.0451-0.02130.00290.2380.0011-0.02830.00590.01230.1522-9.86817.854-39.42
53.72060.2751-1.25081.5949-0.72582.2012-0.0317-0.062-0.1385-0.0071-0.03140.11280.08350.20280.06320.29250.030.00050.03750.0150.30420.01133.968-30.867
61.4125-3.11972.46278.9162-5.77354.46950.17350.0981-0.2394-0.35350.02830.18020.25430.0643-0.20170.30990.0219-0.00440.2324-0.06680.3799-10.9635.581-48.758
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A2023 - 2116
2X-RAY DIFFRACTION2B2023 - 2116
3X-RAY DIFFRACTION3C2019 - 2116
4X-RAY DIFFRACTION4D2023 - 2116
5X-RAY DIFFRACTION5E2023 - 2116
6X-RAY DIFFRACTION6W392 - 410

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