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- PDB-5vx3: Bcl-xL in complex with Bim-h3Pc-RT -

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Basic information

Entry
Database: PDB / ID: 5vx3
TitleBcl-xL in complex with Bim-h3Pc-RT
Components
  • Bcl-2-like protein 1
  • Bcl-2-like protein 11
KeywordsAPOPTOSIS / Bcl-2 Family / Pro-survival
Function / homology
Function and homology information


BIM-BCL-xl complex / BIM-BCL-2 complex / regulation of developmental pigmentation / RUNX3 regulates BCL2L11 (BIM) transcription / positive regulation of mitochondrial membrane permeability involved in apoptotic process / developmental pigmentation / Activation of BIM and translocation to mitochondria / positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / positive regulation of fibroblast apoptotic process / apoptotic process involved in embryonic digit morphogenesis ...BIM-BCL-xl complex / BIM-BCL-2 complex / regulation of developmental pigmentation / RUNX3 regulates BCL2L11 (BIM) transcription / positive regulation of mitochondrial membrane permeability involved in apoptotic process / developmental pigmentation / Activation of BIM and translocation to mitochondria / positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / positive regulation of fibroblast apoptotic process / apoptotic process involved in embryonic digit morphogenesis / apoptotic process in bone marrow cell / SARS-CoV-1-mediated effects on programmed cell death / The NLRP1 inflammasome / dendritic cell apoptotic process / ear development / dendritic cell proliferation / positive regulation of mononuclear cell proliferation / meiosis I / mammary gland development / BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of T cell apoptotic process / negative regulation of execution phase of apoptosis / negative regulation of dendritic cell apoptotic process / tube formation / regulation of organ growth / negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway / fertilization / cellular response to glucocorticoid stimulus / regulation of mitochondrial membrane permeability / negative regulation of protein localization to plasma membrane / regulation of growth / Bcl-2 family protein complex / myeloid cell homeostasis / NFE2L2 regulating tumorigenic genes / FOXO-mediated transcription of cell death genes / response to cycloheximide / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / NRAGE signals death through JNK / cellular response to alkaloid / STAT5 activation downstream of FLT3 ITD mutants / hepatocyte apoptotic process / thymocyte apoptotic process / negative regulation of reproductive process / negative regulation of developmental process / negative regulation of release of cytochrome c from mitochondria / T cell homeostasis / BH3 domain binding / germ cell development / odontogenesis of dentin-containing tooth / apoptotic mitochondrial changes / positive regulation of IRE1-mediated unfolded protein response / positive regulation of release of cytochrome c from mitochondria / B cell homeostasis / negative regulation of anoikis / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / ectopic germ cell programmed cell death / endomembrane system / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / positive regulation of cell cycle / negative regulation of intrinsic apoptotic signaling pathway / positive regulation of intrinsic apoptotic signaling pathway / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / ovarian follicle development / extrinsic apoptotic signaling pathway in absence of ligand / spleen development / FLT3 Signaling / response to endoplasmic reticulum stress / negative regulation of autophagy / cell-matrix adhesion / release of cytochrome c from mitochondria / post-embryonic development / thymus development / regulation of mitochondrial membrane potential / regulation of cytokinesis / epithelial cell proliferation / response to cytokine / kidney development / cellular response to amino acid stimulus / positive regulation of protein-containing complex assembly / cellular response to gamma radiation / synaptic vesicle membrane / endocytosis / RAS processing / activation of cysteine-type endopeptidase activity involved in apoptotic process / male gonad development / intrinsic apoptotic signaling pathway in response to DNA damage / Signaling by BRAF and RAF1 fusions / positive regulation of neuron apoptotic process / spermatogenesis / microtubule binding / regulation of apoptotic process / nuclear membrane / Interleukin-4 and Interleukin-13 signaling / neuron apoptotic process / defense response to virus / in utero embryonic development / mitochondrial outer membrane / negative regulation of neuron apoptotic process / mitochondrial inner membrane
Similarity search - Function
Apoptosis, Bim N-terminal / Bcl-2-like protein 11 / Bim protein N-terminus / Bcl-x interacting, BH3 domain / Bcl-x interacting, BH3 domain / Apoptosis regulator, Bcl-X / Apoptosis regulator, Bcl-2/ BclX / Apoptosis regulator, Bcl-2, BH4 motif, conserved site / Apoptosis regulator, Bcl-2 family BH4 motif signature. / Apoptosis regulator, Bcl-2 protein, BH4 ...Apoptosis, Bim N-terminal / Bcl-2-like protein 11 / Bim protein N-terminus / Bcl-x interacting, BH3 domain / Bcl-x interacting, BH3 domain / Apoptosis regulator, Bcl-X / Apoptosis regulator, Bcl-2/ BclX / Apoptosis regulator, Bcl-2, BH4 motif, conserved site / Apoptosis regulator, Bcl-2 family BH4 motif signature. / Apoptosis regulator, Bcl-2 protein, BH4 / Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2 family BH4 motif profile. / BH4 Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl-2 family / Bcl-2, Bcl-2 homology region 1-3 / Bcl2-like / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily
Similarity search - Domain/homology
Bcl-2-like protein 11 / Bcl-2-like protein 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.945 Å
AuthorsCowan, A.D. / Brouwer, J.M. / Colman, P.M. / Czabotar, P.E.
Funding support Australia, 3items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)1079706 Australia
National Health and Medical Research Council (NHMRC, Australia)1058331 Australia
National Health and Medical Research Council (NHMRC, Australia)1113133 Australia
CitationJournal: Mol. Cell / Year: 2017
Title: Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design.
Authors: Brouwer, J.M. / Lan, P. / Cowan, A.D. / Bernardini, J.P. / Birkinshaw, R.W. / van Delft, M.F. / Sleebs, B.E. / Robin, A.Y. / Wardak, A. / Tan, I.K. / Reljic, B. / Lee, E.F. / Fairlie, W.D. / ...Authors: Brouwer, J.M. / Lan, P. / Cowan, A.D. / Bernardini, J.P. / Birkinshaw, R.W. / van Delft, M.F. / Sleebs, B.E. / Robin, A.Y. / Wardak, A. / Tan, I.K. / Reljic, B. / Lee, E.F. / Fairlie, W.D. / Call, M.J. / Smith, B.J. / Dewson, G. / Lessene, G. / Colman, P.M. / Czabotar, P.E.
History
DepositionMay 23, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 15, 2017Provider: repository / Type: Initial release
Revision 1.1Nov 29, 2017Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Jan 8, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Bcl-2-like protein 1
B: Bcl-2-like protein 11
C: Bcl-2-like protein 1
D: Bcl-2-like protein 11
E: Bcl-2-like protein 1
F: Bcl-2-like protein 11
G: Bcl-2-like protein 1
H: Bcl-2-like protein 11
hetero molecules


Theoretical massNumber of molelcules
Total (without water)85,44521
Polymers84,6388
Non-polymers80713
Water7,476415
1
A: Bcl-2-like protein 1
B: Bcl-2-like protein 11
E: Bcl-2-like protein 1
F: Bcl-2-like protein 11
hetero molecules


Theoretical massNumber of molelcules
Total (without water)42,69210
Polymers42,3194
Non-polymers3726
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9030 Å2
ΔGint-48 kcal/mol
Surface area16110 Å2
MethodPISA
2
C: Bcl-2-like protein 1
D: Bcl-2-like protein 11
G: Bcl-2-like protein 1
H: Bcl-2-like protein 11
hetero molecules


Theoretical massNumber of molelcules
Total (without water)42,75411
Polymers42,3194
Non-polymers4347
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9380 Å2
ΔGint-49 kcal/mol
Surface area15860 Å2
MethodPISA
Unit cell
Length a, b, c (Å)125.661, 68.811, 102.830
Angle α, β, γ (deg.)90.00, 125.35, 90.00
Int Tables number5
Space group name H-MC121
Components on special symmetry positions
IDModelComponents
11G-479-

HOH

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Components

#1: Protein
Bcl-2-like protein 1 / Bcl2-L-1 / Apoptosis regulator Bcl-X


Mass: 17917.959 Da / Num. of mol.: 4 / Fragment: UNP residues 1-26 and 83-209
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BCL2L1, BCL2L, BCLX / Production host: Escherichia coli (E. coli) / References: UniProt: Q07817
#2: Protein/peptide
Bcl-2-like protein 11 / Bcl2-L-11 / Bcl2-interacting mediator of cell death


Mass: 3241.641 Da / Num. of mol.: 4 / Fragment: UNP residues 141-166 / Mutation: W147R, Y163T / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: O43521
#3: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 13 / Source method: obtained synthetically / Formula: C2H6O2
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 415 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.14 Å3/Da / Density % sol: 42.58 %
Crystal growTemperature: 273 K / Method: vapor diffusion
Details: 200 mM magnesium chloride, 20 % PEG 6000, and 100 mM sodium MES pH 6

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 21, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 1.945→41.936 Å / Num. obs: 52409 / % possible obs: 99 % / Redundancy: 3.7 % / CC1/2: 0.995 / Rmerge(I) obs: 0.09715 / Net I/σ(I): 8.4

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
XDSdata processing
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.945→41.936 Å / SU ML: 0.28 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 25.78
RfactorNum. reflection% reflection
Rfree0.2429 2185 4.17 %
Rwork0.1939 --
obs0.196 52409 99.34 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 1.945→41.936 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5371 0 52 415 5838
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0075559
X-RAY DIFFRACTIONf_angle_d0.8447489
X-RAY DIFFRACTIONf_dihedral_angle_d15.7273240
X-RAY DIFFRACTIONf_chiral_restr0.051771
X-RAY DIFFRACTIONf_plane_restr0.015980
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.9445-1.98680.37381320.3222832X-RAY DIFFRACTION90
1.9868-2.0330.36971310.29033099X-RAY DIFFRACTION100
2.033-2.08390.28991440.25663159X-RAY DIFFRACTION100
2.0839-2.14020.29481330.23243122X-RAY DIFFRACTION100
2.1402-2.20320.25971300.21863172X-RAY DIFFRACTION100
2.2032-2.27430.26481400.21043139X-RAY DIFFRACTION100
2.2743-2.35560.26921320.20213130X-RAY DIFFRACTION100
2.3556-2.44990.25151410.1933172X-RAY DIFFRACTION100
2.4499-2.56140.23651290.18193154X-RAY DIFFRACTION100
2.5614-2.69640.21971440.18443112X-RAY DIFFRACTION100
2.6964-2.86530.25471280.19043211X-RAY DIFFRACTION100
2.8653-3.08650.24091420.18673124X-RAY DIFFRACTION100
3.0865-3.39690.24351390.18683173X-RAY DIFFRACTION100
3.3969-3.88820.2231360.17253162X-RAY DIFFRACTION100
3.8882-4.89750.18481450.1673204X-RAY DIFFRACTION100
4.8975-41.9460.25591390.19273259X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.9365-0.28593.81875.5518-0.76092.001-0.01190.410.1201-0.50590.06030.3474-0.177-0.01720.06980.23150.00890.00250.2166-0.03360.1902-33.3321-17.596925.8228
23.9983-0.88921.95553.024-1.97368.38460.2229-0.09410.2271-0.1606-0.244-0.279-0.15880.5852-0.02230.18610.02680.07180.2184-0.01670.2163-23.6649-20.960628.3868
32.63071.00171.0116.75634.05739.04410.2307-0.3144-0.07510.7395-0.0483-0.19050.1712-0.0431-0.04750.3099-0.03560.02620.20220.05530.2213-20.4113-2.736135.6594
43.5871-1.02810.83543.8780.37832.5372-0.145-0.24840.37810.29940.1116-0.0363-0.4107-0.0030.04010.27010.00230.01440.1528-0.01840.1815-20.758710.554828.2113
52.9657-3.1569-2.5295.61821.33665.3265-0.01040.03790.3441-0.0560.04910.1223-0.3103-0.2866-0.0550.20270.0206-0.0160.1452-0.00760.1606-26.62137.410521.1033
68.4566-5.81976.28055.7588-4.60524.71760.3031-0.03720.2253-1.0327-0.0725-0.47270.42150.6078-0.09720.20760.05220.00190.2906-0.03480.3498-8.6942-3.204920.3158
78.57912.5029-1.38636.10442.54959.11520.0316-0.3376-0.75140.41960.6492-0.68940.5050.3633-0.60920.23980.0806-0.00960.24320.0090.3958-12.3908-9.873528.987
86.3015-4.71471.74356.9212-1.78864.6222-0.2477-0.15640.64111.23510.0245-0.8921-0.85730.44570.17040.5107-0.111-0.10540.4085-0.03240.2861-13.057611.124634.752
97.45090.25562.19244.50060.64316.29880.15910.18620.00090.10890.02210.34240.2544-0.2596-0.16860.22990.02140.03060.17560.00420.1876-16.179752.15753.1374
104.0965-6.1552-2.49799.59622.91426.23730.28750.06170.5439-0.7228-0.1373-0.8916-0.58840.2942-0.08260.2718-0.06550.04480.2180.02620.3493-5.205240.234511.2406
116.3264.1552-0.42083.15550.50892.48460.166-0.2231-0.943-0.178-0.036-1.68250.17740.5331-0.01340.55020.04090.08720.36350.05360.39783.82926.28869.2402
126.45974.72346.70977.92955.01247.0251-0.08431.1017-1.4022-0.7854-0.3337-1.3873-0.18620.56360.03740.66110.11810.00240.4337-0.08080.6617-3.609117.92716.7239
135.68062.2340.46815.97512.91513.75360.29620.3568-0.88640.0757-0.19130.02990.92520.08390.17470.36860.0072-0.02590.2014-0.02520.3137-14.250318.697513.3136
143.53830.44610.79670.88020.7445.19740.07190.0339-0.2432-0.0579-0.0139-0.31060.61270.3643-0.0490.35250.0030.06530.1407-0.00910.2512-8.671228.366314.2415
154.2516-0.5999-0.06825.26763.31632.4810.00680.3595-0.352-0.3208-0.1589-0.05470.3708-0.29580.18650.2679-0.0210.01750.1488-0.03970.1991-18.700926.65399.8313
162.56052.34580.09952.62831.71165.8269-0.0632-0.41540.05080.76810.18530.1830.04470.0024-0.13470.2767-0.0242-0.01260.2905-0.03190.273-13.37337.329427.0123
173.18072.1532-1.35032.4593-1.2626.7087-0.2516-0.43160.78110.32310.5571-0.6339-0.12950.5758-0.12630.4045-0.07270.00080.2561-0.0440.3829-6.455443.911120.5178
189.50735.08971.33583.62552.62387.67880.0141-0.4366-0.6915-0.38190.0065-1.33380.47130.4909-0.03010.27340.0159-0.0430.34440.07150.4213-0.859525.160418.3101
199.5964-4.6904-7.47883.76614.25735.7502-0.08240.232-0.4495-0.0347-0.2160.0924-0.0647-0.3070.3230.255-0.02930.00950.14780.01710.2057-25.3586-4.386223.5559
203.3052-0.1582-0.21523.86683.1882.6815-0.06730.0034-0.3348-0.157-0.03150.553-0.6959-0.7016-0.10810.30810.09050.00230.25680.00040.2928-36.64820.257927.9512
214.42141.7894-0.97832.61261.01872.24810.0144-0.13040.3270.34410.26890.7236-0.289-0.1071-0.42930.1555-0.00060.01740.2029-0.03410.2037-39.4577-14.211233.6201
222.0212-2.1272.47013.3852-3.67784.06-0.0088-0.22370.11560.27210.36210.40250.3859-0.5812-0.30270.4019-0.01790.0030.29630.07790.3147-39.406-27.886442.0935
235.948-1.4614-0.22573.6750.28523.34370.13460.1242-1.1024-0.2653-0.09630.42480.7216-0.2069-0.01190.3491-0.0739-0.01620.232-0.02140.3934-37.3037-36.69428.7291
242.7164-0.7003-0.3392.5179-0.50022.4622-0.1322-0.0628-0.3267-0.00590.0820.44340.3408-0.3273-0.03410.2214-0.0468-0.03250.2165-0.01930.2417-41.3405-26.451130.2496
255.1419-1.34120.15083.3318-0.36722.43060.18350.2577-0.5761-0.3234-0.06070.36130.2106-0.09030.0040.28110.0082-0.03780.2023-0.08750.2107-33.6225-28.797722.7073
265.2164-3.8276-0.43926.9542-0.14773.09220.0990.98920.0843-1.25780.05270.7363-0.0784-0.8796-0.05570.2974-0.0131-0.19150.5005-0.0060.4704-51.4374-18.269620.676
272.6434-1.2236-2.54342.7372.13522.92290.226-0.30360.71470.1081-0.16710.7254-0.394-0.5670.00860.2110.0414-0.05650.3555-0.08840.5032-47.499-10.64429.5734
285.9781-2.2242-0.58771.22721.62195.6994-0.0544-0.4725-0.78690.77570.0441.03820.5246-0.59-0.01460.263-0.11340.07610.37050.03630.4515-47.6762-29.406636.4882
296.9524-3.2959-6.0113.56343.75645.43230.15570.351-0.2765-0.3597-0.44260.1909-0.8054-0.41970.2160.24920.01630.02570.19520.00260.2386-15.966538.518210.4334
300.83832.5527-0.78437.6379-2.66960.9488-0.20520.1233-0.1916-0.51480.0436-0.30520.27170.15860.16220.30140.02280.0060.2077-0.01010.2055-12.017244.9998-5.4198
312.5347-1.9482-2.51143.813.10964.98910.0366-0.18160.353-0.33370.1425-0.1054-0.51510.3654-0.10760.4654-0.0158-0.02080.26160.02560.4028-7.155268.7469-3.0718
321.7537-0.09030.00362.99970.89333.1608-0.05620.13410.2948-0.4455-0.12570.4645-0.5571-0.17960.15220.23550.0292-0.05860.17510.0180.2503-17.423363.1292-3.4229
339.2551-3.07920.18632.73680.4437.89930.21710.407-0.425-0.7005-0.17010.51580.8664-0.1458-0.0520.4056-0.0101-0.13070.20860.01550.2571-21.842948.0906-13.1456
343.22170.58050.39876.69675.57856.4097-0.20940.36010.347-0.73380.07060.24880.16930.52640.08810.59620.0237-0.0330.43890.08910.1528-10.816663.8387-14.9718
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 20 )
2X-RAY DIFFRACTION2chain 'A' and (resid 21 through 25 )
3X-RAY DIFFRACTION3chain 'A' and (resid 26 through 105 )
4X-RAY DIFFRACTION4chain 'A' and (resid 106 through 156 )
5X-RAY DIFFRACTION5chain 'A' and (resid 157 through 177 )
6X-RAY DIFFRACTION6chain 'A' and (resid 178 through 186 )
7X-RAY DIFFRACTION7chain 'A' and (resid 187 through 196 )
8X-RAY DIFFRACTION8chain 'B' and (resid 84 through 104 )
9X-RAY DIFFRACTION9chain 'C' and (resid 0 through 25 )
10X-RAY DIFFRACTION10chain 'C' and (resid 26 through 100 )
11X-RAY DIFFRACTION11chain 'C' and (resid 101 through 105 )
12X-RAY DIFFRACTION12chain 'C' and (resid 106 through 112 )
13X-RAY DIFFRACTION13chain 'C' and (resid 113 through 130 )
14X-RAY DIFFRACTION14chain 'C' and (resid 131 through 156 )
15X-RAY DIFFRACTION15chain 'C' and (resid 157 through 177 )
16X-RAY DIFFRACTION16chain 'C' and (resid 178 through 186 )
17X-RAY DIFFRACTION17chain 'C' and (resid 187 through 196 )
18X-RAY DIFFRACTION18chain 'D' and (resid 85 through 106 )
19X-RAY DIFFRACTION19chain 'E' and (resid -4 through 20 )
20X-RAY DIFFRACTION20chain 'E' and (resid 21 through 25 )
21X-RAY DIFFRACTION21chain 'E' and (resid 26 through 100 )
22X-RAY DIFFRACTION22chain 'E' and (resid 101 through 105 )
23X-RAY DIFFRACTION23chain 'E' and (resid 106 through 130 )
24X-RAY DIFFRACTION24chain 'E' and (resid 131 through 156 )
25X-RAY DIFFRACTION25chain 'E' and (resid 157 through 177 )
26X-RAY DIFFRACTION26chain 'E' and (resid 178 through 186 )
27X-RAY DIFFRACTION27chain 'E' and (resid 187 through 197 )
28X-RAY DIFFRACTION28chain 'F' and (resid 85 through 106 )
29X-RAY DIFFRACTION29chain 'G' and (resid -4 through 20 )
30X-RAY DIFFRACTION30chain 'G' and (resid 21 through 100 )
31X-RAY DIFFRACTION31chain 'G' and (resid 101 through 118 )
32X-RAY DIFFRACTION32chain 'G' and (resid 119 through 177 )
33X-RAY DIFFRACTION33chain 'G' and (resid 178 through 197 )
34X-RAY DIFFRACTION34chain 'H' and (resid 84 through 106 )

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