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- PDB-5vr3: Crystal structure of the BRS domain of BRAF -

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Basic information

Entry
Database: PDB / ID: 5vr3
TitleCrystal structure of the BRS domain of BRAF
ComponentsBRAF
KeywordsTRANSFERASE / BRAF
Function / homology
Function and homology information


Signalling to p38 via RIT and RIN / ARMS-mediated activation / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / positive regulation of D-glucose transmembrane transport / establishment of protein localization to membrane / Negative feedback regulation of MAPK pathway / Frs2-mediated activation / MAP kinase kinase activity / positive regulation of peptidyl-serine phosphorylation ...Signalling to p38 via RIT and RIN / ARMS-mediated activation / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / positive regulation of D-glucose transmembrane transport / establishment of protein localization to membrane / Negative feedback regulation of MAPK pathway / Frs2-mediated activation / MAP kinase kinase activity / positive regulation of peptidyl-serine phosphorylation / MAP kinase kinase kinase activity / postsynaptic modulation of chemical synaptic transmission / animal organ morphogenesis / cellular response to calcium ion / protein modification process / RAF activation / Signaling by high-kinase activity BRAF mutants / Spry regulation of FGF signaling / MAP2K and MAPK activation / positive regulation of protein phosphorylation / epidermal growth factor receptor signaling pathway / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / MAPK cascade / Negative regulation of MAPK pathway / Signaling by BRAF and RAF1 fusions / scaffold protein binding / cell body / positive regulation of ERK1 and ERK2 cascade / protein kinase activity / protein phosphorylation / non-specific serine/threonine protein kinase / postsynapse / neuron projection / protein serine kinase activity / protein serine/threonine kinase activity / positive regulation of gene expression / calcium ion binding / negative regulation of apoptotic process / glutamatergic synapse / signal transduction / mitochondrion / zinc ion binding / ATP binding / metal ion binding / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
: / Raf-like Ras-binding domain / Raf-like Ras-binding / Ras-binding domain (RBD) profile. / Raf-like Ras-binding domain / Diacylglycerol/phorbol-ester binding / Phorbol esters/diacylglycerol binding domain (C1 domain) / : / Zinc finger phorbol-ester/DAG-type signature. / Zinc finger phorbol-ester/DAG-type profile. ...: / Raf-like Ras-binding domain / Raf-like Ras-binding / Ras-binding domain (RBD) profile. / Raf-like Ras-binding domain / Diacylglycerol/phorbol-ester binding / Phorbol esters/diacylglycerol binding domain (C1 domain) / : / Zinc finger phorbol-ester/DAG-type signature. / Zinc finger phorbol-ester/DAG-type profile. / Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains) / Protein kinase C-like, phorbol ester/diacylglycerol-binding domain / C1-like domain superfamily / Protein tyrosine and serine/threonine kinase / Serine-threonine/tyrosine-protein kinase, catalytic domain / Ubiquitin-like domain superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
non-specific serine/threonine protein kinase / Serine/threonine-protein kinase B-raf
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / AB INITIO PHASING / Resolution: 2.102 Å
AuthorsThevakumaran, N. / Maisonneuve, P. / Kurinov, I. / Lavoie, H. / Marullo, S.A. / Sahmi, M. / Jin, T. / Therrien, M. / Sicheri, F.
CitationJournal: Nature / Year: 2018
Title: MEK drives BRAF activation through allosteric control of KSR proteins.
Authors: Lavoie, H. / Sahmi, M. / Maisonneuve, P. / Marullo, S.A. / Thevakumaran, N. / Jin, T. / Kurinov, I. / Sicheri, F. / Therrien, M.
History
DepositionMay 10, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 14, 2018Provider: repository / Type: Initial release
Revision 1.1Feb 28, 2018Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Mar 7, 2018Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Mar 13, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: BRAF
hetero molecules


Theoretical massNumber of molelcules
Total (without water)9,6402
Polymers9,5441
Non-polymers961
Water23413
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)41.563, 41.563, 98.855
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number152
Space group name H-MP3121
Components on special symmetry positions
IDModelComponents
11A-311-

HOH

21A-313-

HOH

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Components

#1: Protein BRAF


Mass: 9544.413 Da / Num. of mol.: 1 / Fragment: UNp residues 38-116
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BRAF / Plasmid: pGEX 2T / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: G3RLM7, UniProt: P15056*PLUS, non-specific serine/threonine protein kinase
#2: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.58 Å3/Da / Density % sol: 52.44 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 / Details: 0.1 M Bis-Tris pH 6.5, 2.0 M ammonium sulphate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 29, 2016
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.1→50 Å / Num. obs: 5773 / % possible obs: 92.8 % / Redundancy: 21.9 % / CC1/2: 0.986 / Net I/σ(I): 75
Reflection shellResolution: 2.1→2.14 Å / Redundancy: 10.7 % / Rmerge(I) obs: 0.254 / Mean I/σ(I) obs: 3.61 / CC1/2: 0.986 / % possible all: 49.2

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Processing

Software
NameVersionClassification
PHENIX(1.10_2155: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
Arcimboldophasing
RefinementMethod to determine structure: AB INITIO PHASING / Resolution: 2.102→35.995 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 0.69 / Phase error: 36.55 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2244 492 4.9 %
Rwork0.2095 --
obs0.2102 10045 90.12 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.102→35.995 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms492 0 5 13 510
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.008504
X-RAY DIFFRACTIONf_angle_d0.8682
X-RAY DIFFRACTIONf_dihedral_angle_d19.147311
X-RAY DIFFRACTIONf_chiral_restr0.04476
X-RAY DIFFRACTIONf_plane_restr0.00487
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1015-2.3130.3203730.26171742X-RAY DIFFRACTION65
2.313-2.64760.33231430.26832537X-RAY DIFFRACTION97
2.6476-3.33530.26491260.24192640X-RAY DIFFRACTION100
3.3353-35.99990.19861500.18742634X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.72580.759-1.28496.6366-1.73362.8447-0.2617-0.58710.47220.2329-0.33080.2875-1.38460.06380.44640.37570.03580.02010.7816-0.09090.403525.814218.269625.2149
25.4116-5.4301-2.08226.18552.88742.66820.70461.4469-0.89811.3387-1.44781.26450.0688-1.82490.72641.5719-0.3976-0.02631.2934-0.02390.744222.251814.98880.4258
34.66322.3377-2.92964.087-2.82612.528-0.744-0.2282-0.0529-0.46250.2954-0.12821.9917-0.57760.34380.80910.011-0.02940.6852-0.0290.388226.54389.548321.4006
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 41 through 67 )
2X-RAY DIFFRACTION2chain 'A' and (resid 68 through 75 )
3X-RAY DIFFRACTION3chain 'A' and (resid 76 through 102 )

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