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Open data
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Basic information
Entry | Database: PDB / ID: 5vpn | |||||||||||||||||||||
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Title | E. coli Quinol fumarate reductase FrdA E245Q mutation | |||||||||||||||||||||
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![]() | OXIDOREDUCTASE / quinol fumarate reductase / fumarate reduction / membrane protein / Complex II Superfamily | |||||||||||||||||||||
Function / homology | ![]() : / : / succinate dehydrogenase activity / fermentation / fumarate metabolic process / succinate dehydrogenase / anaerobic electron transport chain / succinate dehydrogenase (quinone) activity / anaerobic respiration / 3 iron, 4 sulfur cluster binding ...: / : / succinate dehydrogenase activity / fermentation / fumarate metabolic process / succinate dehydrogenase / anaerobic electron transport chain / succinate dehydrogenase (quinone) activity / anaerobic respiration / 3 iron, 4 sulfur cluster binding / bacterial-type flagellum assembly / tricarboxylic acid cycle / FAD binding / 2 iron, 2 sulfur cluster binding / flavin adenine dinucleotide binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / DNA damage response / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | |||||||||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||||||||
![]() | Starbird, C.A. / Maklashina, E. / Sharma, P. / Qualls-Histed, S. / Cecchini, G. / Iverson, T.M. | |||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase. Authors: Starbird, C.A. / Maklashina, E. / Sharma, P. / Qualls-Histed, S. / Cecchini, G. / Iverson, T.M. | |||||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 872 KB | Display | ![]() |
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PDB format | ![]() | 723.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 901.5 KB | Display | ![]() |
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Full document | ![]() | 928 KB | Display | |
Data in XML | ![]() | 44.1 KB | Display | |
Data in CIF | ![]() | 64.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1l0vS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 2 types, 4 molecules AEBF
#1: Protein | Mass: 64339.711 Da / Num. of mol.: 2 / Mutation: E245Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: frdA, b4154, JW4115 / Production host: ![]() ![]() #2: Protein | Mass: 27021.885 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: A0A076YHN1, UniProt: P0AC49*PLUS, succinate dehydrogenase |
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-Fumarate reductase subunit ... , 2 types, 4 molecules CGDH
#3: Protein | Mass: 14898.773 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: frdC, b4152, JW4113 / Production host: ![]() ![]() #4: Protein | Mass: 13118.870 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: frdD, b4151, JW4112 / Production host: ![]() ![]() |
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-Non-polymers , 4 types, 8 molecules ![](data/chem/img/FAD.gif)
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#5: Chemical | #6: Chemical | #7: Chemical | #8: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.25 Å3/Da / Density % sol: 71.07 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: Crystals of the QFR-FrdAE245Q mutant were grown using the hanging-drop vapor diffusion method in droplets containing protein solution (15 mg/ml QFR FrdAE245Q in 25 mM Tris-HCl pH 7.4, 1 mM ...Details: Crystals of the QFR-FrdAE245Q mutant were grown using the hanging-drop vapor diffusion method in droplets containing protein solution (15 mg/ml QFR FrdAE245Q in 25 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.02 % C12E9) and the reservoir solution (275 mM NaMalonate, 19% polyethylene glycol 6000, 100 mM NaCitrate pH 4.0, 1 mM EDTA and 0.001% dithiothreitol) in a 1:1 ratio. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 19, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 4.22→50 Å / Num. obs: 28494 / % possible obs: 96.8 % / Redundancy: 5.5 % / Net I/σ(I): 1.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1L0V Resolution: 4.2232→22.962 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 32.59
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 469.59 Å2 / Biso mean: 214.5661 Å2 / Biso min: 67.98 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 4.2232→22.962 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: -3.395 Å / Origin y: -38.8332 Å / Origin z: 6.8835 Å
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Refinement TLS group |
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