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- PDB-5voi: X-ray crystal structure of bacterial RNA polymerase and pyrG prom... -

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Basic information

Entry
Database: PDB / ID: 5voi
TitleX-ray crystal structure of bacterial RNA polymerase and pyrG promoter complex
Components
  • (DNA-directed RNA polymerase subunit ...) x 4
  • (PyrG promoter) x 2
  • RNA polymerase sigma factor SigA
Keywordstranscription/dna/rna / Thermus thermophilus / RNA polymerase / reiterative transcription / holoenzyme / transcription-dna-rna complex
Function / homology
Function and homology information


sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
: / DNA-directed RNA polymerase subunit beta', hybrid domain / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3 / RNA polymerase Rpb2, domain 2 / RNA polymerase II, Rpb2 subunit, wall domain / RNA polymerase subunit, RPB6/omega / Eukaryotic RPB6 RNA polymerase subunit / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1 funnel domain / RNA polymerase, RBP11-like subunit ...: / DNA-directed RNA polymerase subunit beta', hybrid domain / Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3 / RNA polymerase Rpb2, domain 2 / RNA polymerase II, Rpb2 subunit, wall domain / RNA polymerase subunit, RPB6/omega / Eukaryotic RPB6 RNA polymerase subunit / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1 funnel domain / RNA polymerase, RBP11-like subunit / Enzyme I; Chain A, domain 2 / Topoisomerase I; Chain A, domain 4 / DNA-directed RNA polymerase, insert domain / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / RNA Polymerase Alpha Subunit; Chain A, domain 2 / : / RNA polymerase sigma-70 region 1.2 / Sigma-70 factor, region 1.2 / RNA polymerase sigma-70 region 3 / Sigma-70 region 3 / Sigma-70 factors family signature 2. / RNA polymerase sigma-70 / RNA polymerase sigma-70 region 4 / Sigma-70, region 4 / RNA polymerase sigma-70 region 2 / RNA polymerase sigma-70 like domain / Sigma-70 region 2 / RNA polymerase sigma factor, region 2 / RNA polymerase sigma factor, region 3/4-like / Gyrase A; domain 2 / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase beta subunit, bacterial-type / DNA-directed RNA polymerase, alpha subunit / Beta Complex / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, N-terminal / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase Rpb2, domain 2 / RNA polymerase I subunit A N-terminus / RNA polymerase, RBP11-like subunit / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Winged helix-like DNA-binding domain superfamily / Alpha-Beta Complex / Beta Barrel / 2-Layer Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA polymerase sigma factor SigA / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
Bacillus subtilis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsMurakami, K.S. / Shin, Y. / Turnbough Jr, C.L. / Molodtsov, V.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM087350 United States
CitationJournal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2017
Title: X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.
Authors: Murakami, K.S. / Shin, Y. / Turnbough, C.L. / Molodtsov, V.
History
DepositionMay 2, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 24, 2017Provider: repository / Type: Initial release
Revision 1.1Jul 12, 2017Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name
Revision 1.2Aug 9, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Sep 20, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Dec 13, 2017Group: Advisory / Category: database_PDB_caveat
Revision 1.5Jan 1, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.6Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.7Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-directed RNA polymerase subunit alpha
B: DNA-directed RNA polymerase subunit alpha
C: DNA-directed RNA polymerase subunit beta
D: DNA-directed RNA polymerase subunit beta'
E: DNA-directed RNA polymerase subunit omega
F: RNA polymerase sigma factor SigA
G: PyrG promoter
H: PyrG promoter
hetero molecules


Theoretical massNumber of molelcules
Total (without water)441,96813
Polymers441,7648
Non-polymers2045
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area51260 Å2
ΔGint-203 kcal/mol
Surface area141630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)183.180, 103.474, 294.444
Angle α, β, γ (deg.)90.00, 99.14, 90.00
Int Tables number5
Space group name H-MC121

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Components

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DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules ABCDE

#1: Protein DNA-directed RNA polymerase subunit alpha / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 35056.164 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) (bacteria)
Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: Q72I32, DNA-directed RNA polymerase
#2: Protein DNA-directed RNA polymerase subunit beta / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 125436.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE9, DNA-directed RNA polymerase
#3: Protein DNA-directed RNA polymerase subunit beta' / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 170997.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE8, DNA-directed RNA polymerase
#4: Protein DNA-directed RNA polymerase subunit omega / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 11533.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q8RQE7, DNA-directed RNA polymerase

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Protein , 1 types, 1 molecules F

#5: Protein RNA polymerase sigma factor SigA


Mass: 48598.031 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Strain: HB8 / ATCC 27634 / DSM 579 / References: UniProt: Q5SKW1

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DNA chain , 2 types, 2 molecules GH

#6: DNA chain PyrG promoter


Mass: 6674.347 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Bacillus subtilis (bacteria)
#7: DNA chain PyrG promoter


Mass: 8412.417 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Bacillus subtilis (bacteria)

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Non-polymers , 2 types, 5 molecules

#8: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#9: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.12 Å3/Da / Density % sol: 60.55 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.98 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 7, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98 Å / Relative weight: 1
ReflectionResolution: 2.8→30 Å / Num. obs: 132496 / % possible obs: 98.2 % / Redundancy: 3.3 % / Net I/σ(I): 8.6

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Processing

Software
NameVersionClassification
PHENIX(1.11.1_2575: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4Q4Z

4q4z


Resolution: 2.8→29.737 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.68 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2356 2000 1.52 %
Rwork0.192 --
obs0.1927 131509 98.15 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.8→29.737 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms27608 819 5 0 28432
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01429035
X-RAY DIFFRACTIONf_angle_d1.47639417
X-RAY DIFFRACTIONf_dihedral_angle_d25.53111272
X-RAY DIFFRACTIONf_chiral_restr0.0884438
X-RAY DIFFRACTIONf_plane_restr0.0115031
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.8-2.870.41861250.34977969X-RAY DIFFRACTION85
2.87-2.94750.38181480.31068940X-RAY DIFFRACTION95
2.9475-3.03420.33911420.27819183X-RAY DIFFRACTION99
3.0342-3.1320.31771330.26799296X-RAY DIFFRACTION99
3.132-3.24380.30591480.26829338X-RAY DIFFRACTION99
3.2438-3.37350.27151400.22359337X-RAY DIFFRACTION100
3.3735-3.52680.23871530.2089348X-RAY DIFFRACTION100
3.5268-3.71240.26241290.19589333X-RAY DIFFRACTION99
3.7124-3.94450.23271510.18039358X-RAY DIFFRACTION100
3.9445-4.24830.19581410.16089421X-RAY DIFFRACTION100
4.2483-4.67430.19981470.14699407X-RAY DIFFRACTION100
4.6743-5.34730.19621470.15679434X-RAY DIFFRACTION100
5.3473-6.72420.22141530.18389481X-RAY DIFFRACTION100
6.7242-29.73820.18161430.15329664X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.81010.4784-0.08851.7688-0.23171.92760.10890.2907-0.112-0.03580.03110.0993-0.24980.0709-0.12750.64820.0129-0.03680.99230.00290.3082641.56117.9475718.3679
20.2594-0.3505-0.47760.69010.33061.25170.0327-0.27920.19240.27980.0286-0.1740.13180.3795-0.06130.4793-0.0318-0.0770.6735-0.09720.3901658.91759.0371695.8795
31.91570.74610.20851.85181.03454.09170.1798-1.14070.05970.62860.0255-0.47720.0820.6871-0.1960.4706-0.0623-0.11071.1449-0.02780.4607672.47514.1652699.9155
41.6108-0.9643-0.48853.50620.28911.2577-0.0704-0.49140.35960.14490.2227-0.5528-0.08350.3481-0.17110.4551-0.1099-0.09560.8879-0.11450.4475669.92858.4495692.063
56.08111.1196-4.19134.8147-0.33935.3135-0.1821-0.2983-0.31330.56510.15330.51680.3041-0.49970.0320.478-0.0214-0.05280.81190.03920.3024635.2463.8369714.8841
62.5525-0.30810.05791.2222-0.30762.0087-0.0612-0.35920.3140.3758-0.0678-0.1179-0.41480.62630.1330.6692-0.12380.0270.7916-0.2130.3613645.807515.926708.9701
73.4994-0.2866-0.87351.4596-0.05632.2548-0.0232-0.30170.4932-0.06030.09870.0062-0.6403-0.2549-0.05840.64190.04780.02590.5168-0.10470.3239628.132523.7537702.3645
83.06850.94460.35485.30631.09874.45090.3069-0.60970.15810.2726-0.15210.3405-0.4613-0.3432-0.15110.64540.20470.10620.9988-0.04230.3933601.548519.5895712.2622
94.2647-1.60750.52611.90220.08212.1695-0.206-1.05140.52090.24670.22490.2687-0.5594-0.7081-0.01880.85990.28280.11781.0192-0.13190.5613602.538922.7415714.5093
104.9243-0.93430.89433.6563-0.41341.02160.0175-0.08450.407-0.59560.2757-0.3472-0.36840.3451-0.30580.8081-0.06630.07770.529-0.17310.3894633.553523.0625702.1381
114.6332-0.5262-2.37862.2695-0.37964.53890.11970.334-0.168-0.06150.0153-0.36440.12440.1704-0.1160.29210.105-0.07520.3111-0.00210.4916671.3859-25.3966653.0667
122.4379-0.1067-0.30892.77170.45735.2162-0.52621.0774-0.5504-1.17020.50110.03281.1679-0.12130.01691.0834-0.27810.05180.8128-0.07420.6231645.3429-37.4455632.0242
132.0571-0.0684-0.12441.33960.3711.22920.00240.243-0.248-0.0236-0.0675-0.05090.12460.0240.07290.36430.0965-0.00790.24780.03880.3768651.3745-25.9515660.2404
141.99550.2277-0.50641.0848-0.08981.3160.0167-0.21650.02410.0536-0.0398-0.3012-0.06340.5050.03860.3198-0.0021-0.06120.4092-0.02380.283663.43190.9541674.0907
151.12850.1247-0.34030.3909-0.07631.66980.0322-0.14130.08870.1137-0.03960.015-0.14630.2283-0.02350.2423-0.0258-0.00720.1399-0.06650.2971640.53346.1752670.6644
160.7097-0.9283-1.11540.49250.77661.39570.2150.8268-0.2463-0.3326-0.42470.0801-0.1617-0.53140.180.6658-0.00180.03641.5242-0.16380.6017659.8125-9.4774592.9423
171.3055-0.251-0.02870.562-0.15661.60860.03580.0097-0.12680.03420.00050.16290.081-0.147-0.02790.257-0.03640.00190.1372-0.01880.3659620.06113.6096661.4672
181.59530.4690.22872.90360.23492.548-0.0127-0.18310.72060.15480.07820.1228-0.77090.2027-0.05230.5678-0.03540.01940.2478-0.0620.6373626.376437.131663.4847
194.952-3.9297-2.04756.7273-2.08594.6625-0.6088-0.74941.26360.8530.2909-0.5549-0.95930.5240.32150.6137-0.05510.03090.3401-0.15480.6779633.986937.7786672.5929
203.61132.03490.41711.95470.55331.8847-0.06510.49390.7646-0.53170.3667-0.0084-0.61450.292-0.30190.8965-0.02540.00190.36950.06250.8371627.345147.6877653.5194
211.05350.8609-0.34771.9209-2.24223.31680.12140.82870.0824-0.23350.0101-0.0256-0.3335-0.2594-0.10730.96830.08680.07811.6383-0.23970.5782666.1432-2.8438595.3009
221.0602-0.34490.11392.18161.28671.7380.1830.5591-0.2122-0.1371-0.094-0.3477-0.04320.2429-0.07910.3876-0.05610.0390.774-0.00970.4209674.5958-2.6686628.2716
233.95684.9404-0.68526.1524-0.85160.1087-0.19880.1156-0.1125-0.27720.0586-0.6536-0.07370.20110.12480.7166-0.03930.03710.5972-0.03921.146668.628949.6104648.89
245.96261.15884.27735.0112-2.04187.00130.95480.5832-0.579-0.29370.43010.72490.7967-0.512-1.37571.3125-0.6467-0.2291.8263-0.25961.7699620.4753-17.4552622.3238
253.41760.56610.9092.5816-1.05963.00580.49910.459-1.1263-0.21190.2019-0.17030.19810.1494-0.68570.3831-0.12570.02340.5894-0.18240.6832637.6725-4.3532641.5438
262.0005-4.78152.39539.3465-1.06052.00040.36060.1144-1.09-0.0439-0.1502-0.89341.27140.9161-0.22640.89930.00040.04690.8052-0.09960.6165654.75470.6325656.9238
272.01140.9478-2.5674.64660.22766.40990.21350.3516-0.49240.04920.132-0.46960.53250.4833-0.43490.65030.0924-0.07820.9215-0.11180.6292670.9675-18.526613.0836
280.23630.8930.08833.4102-0.10835.3286-0.80070.4531-0.8401-0.6803-0.0586-1.34490.22981.14790.84460.7565-0.27730.1830.8680.03421.1791639.4893-17.0099636.4765
297.01344.63013.88449.4752-1.33036.1241-0.41561.4014-1.6533-3.28650.3051-0.14440.4202-0.31990.11741.4269-0.1555-0.0841.3918-0.46640.8698626.1773-12.9789622.3351
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 31 )
2X-RAY DIFFRACTION2chain 'A' and (resid 32 through 68 )
3X-RAY DIFFRACTION3chain 'A' and (resid 69 through 127 )
4X-RAY DIFFRACTION4chain 'A' and (resid 128 through 173 )
5X-RAY DIFFRACTION5chain 'A' and (resid 174 through 191 )
6X-RAY DIFFRACTION6chain 'A' and (resid 192 through 229 )
7X-RAY DIFFRACTION7chain 'B' and (resid 7 through 58 )
8X-RAY DIFFRACTION8chain 'B' and (resid 59 through 95 )
9X-RAY DIFFRACTION9chain 'B' and (resid 96 through 173 )
10X-RAY DIFFRACTION10chain 'B' and (resid 174 through 228 )
11X-RAY DIFFRACTION11chain 'C' and (resid 1 through 136 )
12X-RAY DIFFRACTION12chain 'C' and (resid 137 through 335 )
13X-RAY DIFFRACTION13chain 'C' and (resid 336 through 577 )
14X-RAY DIFFRACTION14chain 'C' and (resid 578 through 897 )
15X-RAY DIFFRACTION15chain 'C' and (resid 898 through 1118 )
16X-RAY DIFFRACTION16chain 'D' and (resid 3 through 582 )
17X-RAY DIFFRACTION17chain 'D' and (resid 583 through 1502 )
18X-RAY DIFFRACTION18chain 'E' and (resid 2 through 46 )
19X-RAY DIFFRACTION19chain 'E' and (resid 47 through 70 )
20X-RAY DIFFRACTION20chain 'E' and (resid 71 through 95 )
21X-RAY DIFFRACTION21chain 'F' and (resid 78 through 202 )
22X-RAY DIFFRACTION22chain 'F' and (resid 203 through 340 )
23X-RAY DIFFRACTION23chain 'F' and (resid 341 through 423 )
24X-RAY DIFFRACTION24chain 'G' and (resid 3 through 7 )
25X-RAY DIFFRACTION25chain 'G' and (resid 8 through 17 )
26X-RAY DIFFRACTION26chain 'G' and (resid 18 through 20 )
27X-RAY DIFFRACTION27chain 'H' and (resid 1 through 10 )
28X-RAY DIFFRACTION28chain 'H' and (resid 11 through 18 )
29X-RAY DIFFRACTION29chain 'H' and (resid 19 through 25 )

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