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- PDB-4g7o: Crystal structure of Thermus thermophilus transcription initiatio... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4g7o | ||||||
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Title | Crystal structure of Thermus thermophilus transcription initiation complex containing 2 nt of RNA | ||||||
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![]() | TRANSCRIPTION / TRANSFERASE/DNA / protein-DNA complex / transcription initiation complex / RNAP-promoter complex / RNAP-promoter open complex / open complex / RPo / RNA polymerase / transcription initiation / transcription Initiation factor sigma A / promoter DNA / nucleoid / TRANSFERASE-DNA complex | ||||||
Function / homology | ![]() sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Y. / Ebright, R.H. | ||||||
![]() | ![]() Title: Structural basis of transcription initiation. Authors: Zhang, Y. / Feng, Y. / Chatterjee, S. / Tuske, S. / Ho, M.X. / Arnold, E. / Ebright, R.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.4 MB | Display | ![]() |
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PDB format | ![]() | 1.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 642.3 KB | Display | ![]() |
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Full document | ![]() | 893.6 KB | Display | |
Data in XML | ![]() | 259.3 KB | Display | |
Data in CIF | ![]() | 351.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4g7hSC ![]() 4g7zC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-DNA-directed RNA polymerase subunit ... , 4 types, 10 molecules ABKLCMDNEO
#1: Protein | Mass: 35056.164 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein | Mass: 125436.539 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Protein | Mass: 170997.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #4: Protein | Mass: 11533.316 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-DNA chain , 2 types, 4 molecules GQHR
#6: DNA chain | Mass: 6480.172 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: template strand #7: DNA chain | Mass: 8421.432 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: nontemplate strand |
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-Protein / RNA chain , 2 types, 4 molecules FPIS
#5: Protein | Mass: 50769.398 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #8: RNA chain | Mass: 629.454 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: RNA strand |
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-Non-polymers , 3 types, 281 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
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#9: Chemical | ChemComp-ZN / #10: Chemical | ChemComp-MG / #11: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.19 Å3/Da / Density % sol: 61.44 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.7 Details: 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 4, 2011 |
Radiation | Monochromator: single crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918 Å / Relative weight: 1 |
Reflection | Resolution: 2.993→50 Å / Num. all: 224790 / Num. obs: 221194 / % possible obs: 98.4 % / Redundancy: 11.4 % / Rmerge(I) obs: 0.174 / Rsym value: 0.174 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 2.993→3.05 Å / Redundancy: 7.9 % / Rmerge(I) obs: 0.87 / Mean I/σ(I) obs: 1.8 / Rsym value: 0.87 / % possible all: 91.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4G7H Resolution: 2.993→46.606 Å / SU ML: 0.45 / σ(F): 0 / Phase error: 27.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.993→46.606 Å
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Refine LS restraints |
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LS refinement shell |
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