Details | Type | Solution-ID | Contents | Details | Label | Solvent system |
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solution1 | 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 100% D2O | 0.5 mM ribonucleic acid, 5 mM KH2PO4/K2HPO4, 0.25 mM EDTASample 100% D2O100% D2Osolution2 | 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 95% H2O/5% D2O | 0.5 mM ribonucleic acid, 5 mM KH2PO4/K2HPO4, 0.25 mM EDTASample 95% H2O/5% D2O95% H2O/5% D2O | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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0.5 mMRNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3') | NA Adenosine, Cytidine,Guanosine, Uridine1 | 5.0 mMpotassium phosphatenatural abundance1 | 0.25 mMEDTAnatural abundance1 | 0.5 mMRNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3') | NA Adenosine, Cytidine,Guanosine, Uridine2 | 5.0 mMpotassium phosphatenatural abundance2 | 0.25 mMEDTAnatural abundance2 | | | | | | | | | | | | | | | | |
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Sample conditions | Conditions-ID | Details | Ionic strength | Label | pH | Pressure (kPa) | Temperature (K) |
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1 | 0.5 mM ribonucleic acid, 5 mM KH2PO4/K2HPO4, 0.25 mM EDTA0.0112 MCondition 100% D2O6 | 1 atm298 K2 | 0.5 mM ribonucleic acid, 5 mM KH2PO4/K2HPO4, 0.25 mM EDTA0.0112 MCondition 95% H2O/5% D2O6 | 1 atm278 K | | | | | | | | | |
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