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- PDB-5v4s: CryoEM Structure of a Prokaryotic Cyclic Nucleotide-Gated Ion Channel -

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Basic information

Entry
Database: PDB / ID: 5v4s
TitleCryoEM Structure of a Prokaryotic Cyclic Nucleotide-Gated Ion Channel
DescriptorTransporter
cation channel family / cyclic nucleotide-binding domain multi-domain protein
KeywordsTRANSPORT PROTEIN / Ion channel / cyclic nucleotide / allostery / vision / olfaction
Specimen sourceLeptospira licerasiae serovar varillal str. var 010 / bacteria
MethodElectron microscopy (4.2 A resolution / Single particle)
AuthorsJames, Z.M. / Borst, A.J. / Haitin, Y. / Frenz, B. / DiMaio, F. / Zagotta, W.N. / Veesler, D.
CitationProc. Natl. Acad. Sci. U.S.A., 2017

Proc. Natl. Acad. Sci. U.S.A., 2017 StrPapers
CryoEM structure of a prokaryotic cyclic nucleotide-gated ion channel.
Zachary M James / Andrew J Borst / Yoni Haitin / Brandon Frenz / Frank DiMaio / William N Zagotta / David Veesler

DateDeposition: Mar 10, 2017 / Release: Apr 12, 2017 / Last modification: Apr 26, 2017

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Assembly

Deposited unit
A: Transporter, cation channel family / cyclic nucleotide-binding domain multi-domain protein
B: Transporter, cation channel family / cyclic nucleotide-binding domain multi-domain protein
C: Transporter, cation channel family / cyclic nucleotide-binding domain multi-domain protein
D: Transporter, cation channel family / cyclic nucleotide-binding domain multi-domain protein


Theoretical massNumber of molelcules
Total (without water)213,8594
Polyers213,8594
Non-polymers00
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Polypeptide(L)
Transporter, cation channel family / cyclic nucleotide-binding domain multi-domain protein


Mass: 53464.785 Da / Num. of mol.: 4
Source: (gene. exp.) Leptospira licerasiae serovar varillal str. var 010 / bacteria
References: UniProt: I0XVQ9

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: LliK / Type: COMPLEX
Specimen supportGrid material: COPPER / Grid mesh size: 400 /inch. / Grid type: Quantifoil R 1.2/1.3
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD

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Processing

3D reconstructionResolution: 4.2 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 18737
Atomic model buildingRef protocol: OTHER / Ref space: REAL

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