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- PDB-6aye: Human apo-TRPML3 channel at pH 7.4 -

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Basic information

Database: PDB / ID: 6aye
TitleHuman apo-TRPML3 channel at pH 7.4
KeywordsTRANSPORT PROTEIN / ion channel / TRP channel / lysosomal
Specimen sourceHomo sapiens / human /
MethodElectron microscopy (4.06 Å resolution / Particle / Single particle) / Transmission electron microscopy
AuthorsZhou, X. / Li, M. / Su, D. / Jia, Q. / Li, H. / Li, X. / Yang, J.
CitationNat. Struct. Mol. Biol., 2017, 24, 1146-1154

Nat. Struct. Mol. Biol., 2017, 24, 1146-1154 Yorodumi Papers
Cryo-EM structures of the human endolysosomal TRPML3 channel in three distinct states.
Xiaoyuan Zhou / Minghui Li / Deyuan Su / Qi Jia / Huan Li / Xueming Li / Jian Yang

Validation Report
SummaryFull reportAbout validation report
DateDeposition: Sep 8, 2017 / Release: Nov 8, 2017
RevisionDateData content typeGroupCategoryItemProviderType
1.0Nov 8, 2017Structure modelrepositoryInitial release
1.1Nov 22, 2017Structure modelDatabase referencescitation_citation.journal_abbrev / _citation.pdbx_database_id_PubMed / _citation.title
1.2Dec 6, 2017Structure modelAuthor supporting evidencepdbx_audit_support_pdbx_audit_support.funding_organization
1.3Dec 20, 2017Structure modelDatabase referencescitation_citation.journal_volume / _citation.page_first / _citation.page_last

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Deposited unit
A: Mucolipin-3
B: Mucolipin-3
C: Mucolipin-3
D: Mucolipin-3

Theoretical massNumber of molelcules
Total (without water)258,5034

TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area (Å2)29740
ΔGint (kcal/M)-253
Surface area (Å2)91200


#1: Protein/peptide

Mass: 64625.785 Da / Num. of mol.: 4 / Source: (gene. exp.) Homo sapiens / human / / Gene: MCOLN3 / Plasmid name: pFastbac1 / Production host: Trichoplusia ni / References: UniProt: Q8TDD5

Experimental details


EM experimentAggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE

Sample preparation

ComponentName: human TRPML3 apo channel at pH 7.4 / Type: COMPLEX / Entity ID: 1 / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Homo sapiens
Source (recombinant)Organism: Trichoplusia ni
Buffer solutionDetails: The pH of the buffer was adjusted to 7.4 by NaOH. / pH: 7.4
Buffer component
IDConc.UnitsNameFormulaBuffer ID
2150mMsodium chlorideNaCl1
SpecimenConc.: 1.9 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 400 / Grid type: Quantifoil holey carbon grid R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 281 kelvins
Details: waiting for 3 seconds before blotting for 4 seconds(double-sided, blot force 1),then the grid was immediately plunged into liquid ethane cooled by liquid-nitrogen

Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELD / Nominal magnification: 22500 / Nominal defocus max: 3000 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 8 sec. / Electron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k)
Image scansSampling size: 5 microns / Dimension width: 7676 / Dimension height: 7420 / Movie frames/image: 32 / Used frames/image: 1-32


EM software
1RELION1.4PARTICLE SELECTIONto automatically pick particles
2EMAN2PARTICLE SELECTIONto semi-automatically pick particles
CTF correctionType: NONE
SymmetryPoint symmetry: C4
3D reconstructionResolution: 4.06 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 43542 / Symmetry type: POINT

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