PDB-5uog: Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate r...

ID or keywords:

Entry

Database: PDB / ID: 5uog

Title

Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase SMc04462 (SmGhrB) from Sinorhizobium meliloti in apo form

Components

NADPH-dependent glyoxylate/hydroxypyruvate reductase

Keywords

OXIDOREDUCTASE / NADPH-dependent glyoxylate/hydroxypyruvate reductase / NADP / NYSGRC / Sinorhizobium meliloti / Structural Genomics / PSI-Biology / New York Structural Genomics Research Consortium

Function / homology

Function and homology information

hydroxypyruvate reductase [NAD(P)H] activity / glyoxylate reductase (NADPH) activity / NAD binding / metal ion binding / cytosol
Similarity search - Function

Biological species

Rhizobium meliloti (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.4 Å

Authors

Shabalin, I.G. / Handing, K.B. / Gasiorowska, O.A. / Cooper, D.R. / Bonanno, J. / Almo, S.C. / Minor, W. / New York Structural Genomics Research Consortium (NYSGRC)

Funding support

United States, 1items

Citation

Journal: Biochemistry / Year: 2018
Title: Structural, Biochemical, and Evolutionary Characterizations of Glyoxylate/Hydroxypyruvate Reductases Show Their Division into Two Distinct Subfamilies.
Authors: Kutner, J. / Shabalin, I.G. / Matelska, D. / Handing, K.B. / Gasiorowska, O. / Sroka, P. / Gorna, M.W. / Ginalski, K. / Wozniak, K. / Minor, W.

History

Deposition	Jan 31, 2017	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Feb 22, 2017	Provider: repository / Type: Initial release
Revision 1.1	Sep 27, 2017	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2	May 16, 2018	Group: Data collection / Database references / Structure summary Category: citation / citation_author ...citation / citation_author / entity / struct Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _entity.pdbx_ec / _struct.pdbx_descriptor
Revision 1.3	Dec 4, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4	Oct 4, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5uog.cif.gz	496 KB	Display	PDBx/mmCIF format
PDB format	pdb5uog.ent.gz	407.9 KB	Display	PDB format
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Validation report

Summary document	5uog_validation.pdf.gz	455 KB	Display	wwPDB validaton report
Full document	5uog_full_validation.pdf.gz	455.6 KB	Display
Data in XML	5uog_validation.xml.gz	52.7 KB	Display
Data in CIF	5uog_validation.cif.gz	78.9 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/uo/5uog ftp://data.pdbj.org/pub/pdb/validation_reports/uo/5uog	HTTPS FTP

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Related structure data

Related structure data	4weqC 4z0pC 5j23SC 5unnC 5v6qC 5v72C 5v7gC 5v7nC S: Starting model for refinement C: citing same article (ref.)
Similar structure data	5v7n-1 5j23-2 5v72-2 2wwr-1 4u6s-1 1wwk-d 5v7g-1 6v89-1 5v6q-2 6bii-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Other databases	TargetTrack: NYSGRC-012132

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: NADPH-dependent glyoxylate/hydroxypyruvate reductase

B: NADPH-dependent glyoxylate/hydroxypyruvate reductase

C: NADPH-dependent glyoxylate/hydroxypyruvate reductase

D: NADPH-dependent glyoxylate/hydroxypyruvate reductase

hetero molecules

148 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: NADPH-dependent glyoxylate/hydroxypyruvate reductase

B: NADPH-dependent glyoxylate/hydroxypyruvate reductase

hetero molecules

defined by author&software
73.9 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: NADPH-dependent glyoxylate/hydroxypyruvate reductase

D: NADPH-dependent glyoxylate/hydroxypyruvate reductase

hetero molecules

defined by author&software
73.9 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	176.583, 176.583, 135.008
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	146
Space group name H-M	H3

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	A
2	1	B
1	2	A
2	2	C
1	3	A
2	3	D
1	4	B
2	4	C
1	5	B
2	5	D
1	6	C
2	6	D

NCS domain segments:

Component-ID: _ / Refine code: _

Dom-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	ARG	ARG	ALA	ALA	A	A	3 - 319	25 - 341
2	1	ARG	ARG	ALA	ALA	B	B	3 - 319	25 - 341
1	2	ARG	ARG	ALA	ALA	A	A	3 - 319	25 - 341
2	2	ARG	ARG	ALA	ALA	C	C	3 - 319	25 - 341
1	3	ARG	ARG	ARG	ARG	A	A	3 - 318	25 - 340
2	3	ARG	ARG	ARG	ARG	D	D	3 - 318	25 - 340
1	4	ARG	ARG	ALA	ALA	B	B	3 - 319	25 - 341
2	4	ARG	ARG	ALA	ALA	C	C	3 - 319	25 - 341
1	5	SER	SER	ARG	ARG	B	B	2 - 318	24 - 340
2	5	SER	SER	ARG	ARG	D	D	2 - 318	24 - 340
1	6	ARG	ARG	ARG	ARG	C	C	3 - 318	25 - 340
2	6	ARG	ARG	ARG	ARG	D	D	3 - 318	25 - 340

NCS ensembles :

ID
1
2
3
4
5
6

#1: Protein	NADPH-dependent glyoxylate/hydroxypyruvate reductase Mass: 36830.148 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhizobium meliloti (strain 1021) (bacteria) Strain: 1021 / Gene: SMc04462 / Plasmid: pSGC-His / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: Q92LZ4, hydroxypyruvate reductase
#2: Chemical	ChemComp-SO4 / SULFATE ION Mass: 96.063 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: SO₄
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 972 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.9 Å³/Da / Density % sol: 57.53 %
Crystal grow	Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 3.5 Details: 0.2 ul of 14 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide, 0.5 mM TCEP and 5 mM NADPH were mixed with 0.2 ul of the MCSG Suite 3 condition #61 (0.1 M ...Details: 0.2 ul of 14 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide, 0.5 mM TCEP and 5 mM NADPH were mixed with 0.2 ul of the MCSG Suite 3 condition #61 (0.1 M Citric acid pH=3.5, 2 M Ammonium sulfate) and equilibrated against 1.5 M NaCl solution in 96 Well 3 drop Crystallization Plate (Swissci). The protein was crystallized with the His-tag

Crystal

Density Matthews: 2.9 Å³/Da / Density % sol: 57.53 %

Crystal grow

Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 3.5
Details: 0.2 ul of 14 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide, 0.5 mM TCEP and 5 mM NADPH were mixed with 0.2 ul of the MCSG Suite 3 condition #61 (0.1 M ...

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 19-ID / Wavelength: 0.97924 Å

Detector

Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 8, 2014

Radiation

Monochromator: Si [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.97924 Å / Relative weight: 1

Reflection twin

Crystal-ID	ID	Operator	Domain-ID	Fraction
1	1	H, K, L	1	0.505
1	1	K, H, -L	2	0.495

Reflection

Resolution: 2.4→50 Å / Num. obs: 61899 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 3.2 % / B_iso Wilson estimate: 56.9 Å² / R_merge(I) obs: 0.061 / R_pim(I) all: 0.04 / R_rim(I) all: 0.073 / R_sym value: 0.061 / Χ²: 1.117 / Net I/av σ(I): 22.7 / Net I/σ(I): 9.8

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. unique obs	CC_1/2	R_pim(I) all	R_rim(I) all	R_sym value	Χ²	% possible all
2.4-2.44	3.2	0.63	2	3113	0.685	0.422	0.761	0.63	0.794	100
2.44-2.49	3.2	0.551			0.695	0.37	0.666		0.861	100
2.49-2.53	3.2	0.467			0.774	0.313	0.564		0.839	100
2.53-2.59	3.2	0.418			0.795	0.281	0.505		0.86	100
2.59-2.64	3.2	0.364			0.837	0.244	0.439		0.83	100
2.64-2.7	3.2	0.308			0.891	0.206	0.371		0.876	100
2.7-2.77	3.2	0.244			0.923	0.163	0.294		0.897	100
2.77-2.85	3.2	0.203			0.948	0.136	0.245		0.886	100
2.85-2.93	3.2	0.165			0.962	0.11	0.199		0.938	100
2.93-3.02	3.2	0.138			0.977	0.092	0.166		0.934	100
3.02-3.13	3.2	0.111			0.983	0.074	0.134		0.995	100
3.13-3.26	3.2	0.089			0.987	0.059	0.107		1.05	100
3.26-3.41	3.2	0.075			0.991	0.05	0.09		1.13	100
3.41-3.58	3.2	0.058			0.995	0.039	0.07		1.239	100
3.58-3.81	3.2	0.049			0.995	0.032	0.059		1.391	100
3.81-4.1	3.2	0.047			0.958	0.032	0.057		1.712	100
4.1-4.52	3.2	0.04			0.997	0.026	0.048		1.669	100
4.52-5.17	3.1	0.037			0.997	0.024	0.044		1.596	100
5.17-6.51	3.1	0.036			0.997	0.024	0.043		1.291	100
6.51-50	3.2	0.035			0.996	0.023	0.042		1.606	99.3

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Processing

Software

Name	Version	Classification
HKL-3000		data collection
HKL-3000		data reduction
HKL-3000		data scaling
HKL-3000		phasing
MOLREP		phasing
REFMAC	5.8.0135	refinement
PDB_EXTRACT	3.22	data extraction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5J23

5j23

Resolution: 2.4→36.78 Å / Cor.coef. F_o:F_c: 0.976 / Cor.coef. F_o:F_c free: 0.955 / SU B: 7.249 / SU ML: 0.104 / SU R Cruickshank DPI: 0.0544 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.054 / ESU R Free: 0.041
Details: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.1904	2939	4.8 %	RANDOM
R_work	0.1355	-	-	-
obs	0.1381	58425	99.93 %	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å

Displacement parameters

B_iso max: 130 Å² / B_iso mean: 57.536 Å² / B_iso min: 22.91 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-12.97 Å²	-0 Å²	-0 Å²
2-	-	-12.97 Å²	-0 Å²
3-	-	-	25.94 Å²

Refinement step

Cycle: final / Resolution: 2.4→36.78 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	9325	0	25	972	10322
B_iso mean	-	-	62.77	53.98	-
Num. residues	-	-	-	-	1271

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.009	0.019	9516
X-RAY DIFFRACTION	r_bond_other_d	0.005	0.02	9388
X-RAY DIFFRACTION	r_angle_refined_deg	1.319	1.983	13021
X-RAY DIFFRACTION	r_angle_other_deg	1.132	3	21434
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	5.533	5	1265
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	34.688	22.819	337
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	13.657	15	1432
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	20.002	15	80
X-RAY DIFFRACTION	r_chiral_restr	0.068	0.2	1611
X-RAY DIFFRACTION	r_gen_planes_refined	0.006	0.021	10761
X-RAY DIFFRACTION	r_gen_planes_other	0.004	0.02	2027

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-ID	Dom-ID	Auth asym-ID	Number	Rms dev position (Å)
1	1	A	38440	0.05
1	2	B	38440	0.05
2	1	A	38560	0.05
2	2	C	38560	0.05
3	1	A	37770	0.05
3	2	D	37770	0.05
4	1	B	37962	0.06
4	2	C	37962	0.06
5	1	B	37970	0.03
5	2	D	37970	0.03
6	1	C	37520	0.06
6	2	D	37520	0.06

LS refinement shell

Resolution: 2.4→2.462 Å / R_factor R_free error: 0 / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.29	204	-
R_work	0.156	4335	-
all	-	4539	-
obs	-	-	99.67 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.9722	0.2506	-0.8934	3.6622	0.0039	3.725	0.0199	0.075	0.0386	-0.2943	-0.0455	-0.6429	-0.2296	0.3292	0.0256	0.2075	0.0001	0.0656	0.264	-0.0056	0.1198	68.335	81.475	80.632
2	2.6392	-0.9339	2.3331	2.4534	-1.2492	3.5704	-0.0055	0.0937	0.23	0.0505	0.0729	-0.3578	-0.1523	0.2594	-0.0674	0.1773	-0.0023	0.026	0.2266	-0.0409	0.1631	70.458	83.56	87.352
3	2.272	-0.2748	-0.9886	2.075	-0.0434	2.6899	-0.0369	-0.2141	0.032	0.1904	0.145	-0.1394	0.2724	0.1286	-0.1082	0.233	0.0302	-0.0003	0.2613	0.0053	0.0377	61.487	77.87	93.219
4	0.5332	-0.171	0.0771	0.8434	0.0973	0.9456	-0.0037	-0.0183	-0.0188	0.1457	0.0388	0.078	0.0997	-0.1297	-0.0352	0.2368	-0.0107	0.0041	0.2386	-0.03	0.0408	61.313	47.293	86.276
5	0.5701	-0.6003	-0.3455	2.1073	0.797	0.3718	0.0318	-0.085	0.0105	0.0543	-0.0014	0.0062	-0.0023	-0.0265	-0.0304	0.2384	-0.0035	0.0168	0.2674	-0.0141	0.0409	62.137	69.423	84.848
6	4.1341	2.263	1.243	5.5178	1.2868	6.1086	-0.099	-0.0149	-0.1992	-0.0429	0.1234	-0.2745	0.068	0.274	-0.0244	0.2319	0.0238	-0.0629	0.2273	0.0044	0.1238	63.78	14.663	68.282
7	8.0049	-1.4707	-0.8053	1.4664	-4.163	15.7791	0.432	-0.2471	0.7687	-0.1067	-0.4101	-0.2119	0.0804	1.898	-0.0218	0.2677	-0.0403	0.056	0.6499	-0.2613	0.3402	73.065	14.889	59.066
8	2.2183	0.0133	0.1629	3.2933	0.6832	3.3672	-0.2138	0.2403	0.0142	-0.2727	0.2583	-0.1471	-0.2016	0.3933	-0.0446	0.2303	-0.0751	-0.0524	0.2551	-0.017	0.0546	61.605	18.594	55.223
9	0.3279	-0.3705	-0.0648	1.4213	-0.4743	0.3171	0.1554	0.0905	-0.0563	-0.2947	-0.1089	0.1705	0.052	-0.0476	-0.0466	0.2494	0.0073	-0.0782	0.2798	-0.0482	0.0371	58.787	50.984	66.097
10	0.0755	0.155	0.1356	0.9506	0.6443	0.4594	0.045	0.0256	-0.0545	-0.2415	0.0252	-0.0645	-0.1008	0.0033	-0.0702	0.3243	0.0032	-0.0672	0.2586	-0.0354	0.0453	62.463	37.88	59.076
11	1.5659	-0.6588	0.2635	1.4662	0.5489	1.3161	-0.0176	0.055	-0.1799	0.0367	-0.0403	0.1188	0.0785	-0.1668	0.0578	0.2326	-0.0198	0.0136	0.2118	0.0301	0.0659	-16.483	33.126	77.24
12	0.4736	-0.6563	0.3138	0.9676	-0.3906	0.5979	0.0577	-0.01	-0.0325	0.0057	0.0831	0.0063	-0.0232	0.056	-0.1408	0.2556	-0.0153	-0.0548	0.2443	-0.0114	0.0596	12.21	20.725	84.583
13	1.3654	-0.4051	0.6411	0.3434	0.4651	8.2657	0.0857	0.101	0.3707	-0.0395	0.0663	-0.1481	-0.5823	0.3635	-0.1521	0.3193	-0.0505	0.0225	0.2545	0.0766	0.2392	20.308	32.522	78.658
14	1.1753	-0.0266	0.0868	0.4605	-0.1634	1.4021	0.1201	0.1576	0.0946	-0.1329	-0.1112	0.0232	-0.074	0.0816	-0.009	0.241	0.0131	-0.0117	0.2612	0.0029	0.0195	14.556	20.153	71.949
15	1.9824	-1.4797	2.0812	2.3729	-2.0871	3.0683	-0.1372	0.06	0.096	0.1938	0.051	-0.0812	-0.2319	0.2111	0.0861	0.2577	-0.0181	-0.0003	0.2209	0.0204	0.0322	-7.469	41.285	80.188
16	2.9341	1.0497	1.1046	3.5141	-0.1291	0.5098	0.1027	0.1459	-0.4284	-0.1734	0.0471	-0.2408	0.0581	0.0686	-0.1499	0.234	0.0013	-0.0126	0.352	-0.0925	0.08	47.771	6.197	97.263
17	1.3223	0.5031	0.411	1.0284	0.5701	1.0596	0.1901	-0.3139	-0.1788	0.1952	-0.05	-0.1231	0.1681	-0.2386	-0.14	0.1864	-0.0455	-0.0606	0.4113	-0.0318	0.0602	41.098	4.601	108.171
18	0.5718	-0.6391	-0.1938	0.7891	0.1544	0.1374	-0.1004	-0.0724	0.0667	0.1083	0.1332	-0.0727	-0.025	-0.0096	-0.0327	0.2915	-0.02	-0.0561	0.2658	-0.0225	0.0746	12.176	22.219	93.374
19	13.5119	2.7612	1.9809	1.1934	1.656	2.7858	0.0287	0.0576	0.5478	0.1554	0.2099	-0.0509	0.321	0.4272	-0.2387	0.3368	0.0703	-0.1842	0.3686	-0.1605	0.2841	25.537	34.775	109.482
20	1.4216	-0.5736	0.5337	0.3384	-0.2064	0.2057	-0.1496	-0.2217	-0.0266	0.1537	0.1654	-0.0594	-0.0715	-0.0507	-0.0157	0.3033	0.0279	-0.0759	0.3147	-0.0636	0.0509	23.132	18.759	105.812

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	3 - 17
2	X-RAY DIFFRACTION	2	A	18 - 60
3	X-RAY DIFFRACTION	3	A	61 - 92
4	X-RAY DIFFRACTION	4	A	93 - 259
5	X-RAY DIFFRACTION	5	A	260 - 319
6	X-RAY DIFFRACTION	6	B	2 - 24
7	X-RAY DIFFRACTION	7	B	25 - 37
8	X-RAY DIFFRACTION	8	B	38 - 93
9	X-RAY DIFFRACTION	9	B	94 - 202
10	X-RAY DIFFRACTION	10	B	205 - 319
11	X-RAY DIFFRACTION	11	C	3 - 79
12	X-RAY DIFFRACTION	12	C	80 - 154
13	X-RAY DIFFRACTION	13	C	155 - 174
14	X-RAY DIFFRACTION	14	C	175 - 278
15	X-RAY DIFFRACTION	15	C	279 - 319
16	X-RAY DIFFRACTION	16	D	-5 - 23
17	X-RAY DIFFRACTION	17	D	24 - 93
18	X-RAY DIFFRACTION	18	D	94 - 167
19	X-RAY DIFFRACTION	19	D	168 - 177
20	X-RAY DIFFRACTION	20	D	178 - 319

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