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Yorodumi- PDB-5tgl: A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5tgl | ||||||
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Title | A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX | ||||||
Components | LIPASE | ||||||
Keywords | HYDROLASE(CARBOXYLIC ESTERASE) | ||||||
Function / homology | Function and homology information triacylglycerol lipase / triglyceride lipase activity / lipid catabolic process / metal ion binding Similarity search - Function | ||||||
Biological species | Rhizomucor miehei (fungus) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3 Å | ||||||
Authors | Brzozowski, A.M. / Derewenda, U. / Derewenda, Z.S. / Dodson, G.G. / Lawson, D. / Turkenburg, J.P. / Bjorkling, F. / Huge-Jensen, B. / Patkar, S.R. / Thim, L. | ||||||
Citation | Journal: Nature / Year: 1991 Title: A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Authors: Brzozowski, A.M. / Derewenda, U. / Derewenda, Z.S. / Dodson, G.G. / Lawson, D.M. / Turkenburg, J.P. / Bjorkling, F. / Huge-Jensen, B. / Patkar, S.A. / Thim, L. #1: Journal: Nature / Year: 1990 Title: A Serine Protease Triad Forms the Catalytic Centre of a Triacylglycerol Lipase Authors: Brady, L. / Brzozowski, A.M. / Derewenda, Z.S. / Dodson, E.J. / Dodson, G. / Tolley, S. / Turkenburg, J.P. / Christiansen, L. / Huge-Jensen, B. / Norskov, L. / Thim, L. / Menge, U. #2: Journal: Lipids / Year: 1988 Title: Rhizomucor Miehei Triglyceride Lipase is Synthesized as a Precursor Authors: Boel, E. / Huge-Jensen, B. / Christensen, M. / Thim, L. / Fiil, N.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5tgl.cif.gz | 36.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5tgl.ent.gz | 13.9 KB | Display | PDB format |
PDBx/mmJSON format | 5tgl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tg/5tgl ftp://data.pdbj.org/pub/pdb/validation_reports/tg/5tgl | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO 34, PRO 209, PRO 229, AND PRO 250 ARE CIS PROLINES. |
-Components
#1: Protein | Mass: 29523.953 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhizomucor miehei (fungus) / References: UniProt: P19515, triacylglycerol lipase |
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#2: Chemical | ChemComp-HEE / |
Sequence details | RESIDUE 156 IN THIS ENTRY IS ASP AS IDENTIFIED BY ELECTRON DENSITY. IN THE PAPER CITED AS REFERENCE ...RESIDUE 156 IN THIS ENTRY IS ASP AS IDENTIFIED |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.51 % | |||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 5.44 Å / Num. obs: 5892 / % possible obs: 87.3 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.079 |
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Reflection shell | *PLUS Highest resolution: 3 Å / Lowest resolution: 3.18 Å / % possible obs: 27.1 % / Redundancy: 2 % / Num. unique obs: 965 / Rmerge(I) obs: 0.186 / Mean I/σ(I) obs: 6.5 |
-Processing
Software |
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Refinement | Rfactor obs: 0.185 / Highest resolution: 3 Å Details: THERE ARE SOME ERRORS IN THE SEQUENCE DUE TO UNCERTAINTY IN THE ORIENTATION OF SIDE CHAINS. THESE DIFFERENCES ARE MINOR AND IN NO WAY COMPROMISE THE OVERALL QUALITY OF THE STRUCTURE OR THE ...Details: THERE ARE SOME ERRORS IN THE SEQUENCE DUE TO UNCERTAINTY IN THE ORIENTATION OF SIDE CHAINS. THESE DIFFERENCES ARE MINOR AND IN NO WAY COMPROMISE THE OVERALL QUALITY OF THE STRUCTURE OR THE SUITABILITY FOR MODELING OR MOLECULAR REPLACEMENT. SEQUENCE ADVISORY NOTICE: DIFFERENCE BETWEEN PIR AND PDB SEQUENCE. PIR ENTRY NAME: A34959 PIR RESIDUE PDB SEQRES NAME NUMBER NAME CHAIN SEQ/INSERT CODE ASP 181 ASN 181 GLU 220 SER 220 | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 3 Å
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Refinement | *PLUS Highest resolution: 3 Å / Rfactor obs: 0.185 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |