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- PDB-5oql: Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum -

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Entry
Database: PDB / ID: 5oql
TitleCryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum
Components
  • (40S ribosomal protein ...) x 15
  • (Putative U3 small nucleolar ...) x 2
  • 35S rRNA
  • Bms1
  • Emg1
  • Faf1
  • Fcf2
  • Imp3
  • KRR1 small subunit processome component
  • Nop1
  • Nop58
  • Periodic tryptophan protein 2-like protein
  • Pre-rRNA-processing protein PNO1
  • Putative U3 snoRNP protein
  • Putative nucleolar proteinNucleolus
  • RNA 3'-terminal phosphate cyclase-like protein
  • RNA cytidine acetyltransferase
  • Rrp9
  • Snu13
  • Sof1
  • U3 small nucleolar RNA-associated protein 11
  • U3 snoRNA
  • Utp10
  • Utp12
  • Utp13
  • Utp14
  • Utp15
  • Utp17
  • Utp18
  • Utp2
  • Utp24
  • Utp3
  • Utp30
  • Utp4
  • Utp6
  • Utp7
KeywordsRIBOSOME / Ribosome biogenesis
Function / homologytRNA methyltransferase complex GCD14 subunit / Ribosome biogenesis protein Bms1, N-terminal / 40S ribosomal protein S11, N-terminal / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / Ribosomal protein S8 superfamily / Nop domain superfamily / WD40-repeat-containing domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / K Homology domain, type 1 superfamily ...tRNA methyltransferase complex GCD14 subunit / Ribosome biogenesis protein Bms1, N-terminal / 40S ribosomal protein S11, N-terminal / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / Ribosomal protein S8 superfamily / Nop domain superfamily / WD40-repeat-containing domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / rRNA-processing protein Fcf1, PIN domain / Ribosomal protein S4e, central domain superfamily / Bms1/Tsr1-type G domain / ATPase family associated with various cellular activities (AAA) / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e / Ribosomal protein S15 / Ribosomal protein S17 / Ribosomal protein S9/S16 / WD domain, G-beta repeat / Ribosomal protein S8 / Ribosomal protein S11 / Ribosomal protein L1p/L10e family / Ribosomal family S4e / Ribosomal S3Ae family / 40S ribosomal protein S4, C-terminal domain / 50S ribosomal protein L30e-like / RNA 3'-terminal phosphate cyclase / Ribosomal protein S15P / Ribosomal protein L7Ae/L8/Nhp2 family / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / WD40 repeat, conserved site / Ribosomal protein S17, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10 / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S4/S9 / Ribosomal protein L1-like / S-adenosyl-L-methionine-dependent methyltransferase / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S7 domain / Ribosomal RNA assembly KRR1 / Periodic tryptophan protein 2 / P-loop containing nucleoside triphosphate hydrolase / 40S ribosomal protein S1/3, eukaryotes / Protein of unknown function DUF4602 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Helix hairpin bin domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / PIN-like domain superfamily / Ribosomal protein S6e / Ribosomal protein S28e / Ribosomal protein S6e, conserved site / Ribosomal protein S24e signature. / GNAT acetyltransferase 2 / Possible tRNA binding domain / Domain of unknown function (DUF4602) / 40S ribosomal protein S4 C-terminus / Ribosomal_S17 N-terminal / Ribosomal protein S7 signature. / Ribosomal protein S8 signature. / Ribosomal protein S11 signature. / Ribosomal protein S12 signature. / Ribosomal protein S17 signature. / Ribosomal protein S9 signature. / Ribosomal protein S15 signature. / Ribosomal protein S4e signature. / Fibrillarin signature. / UTP15 C terminal / Ribosomal protein S6e signature. / Ribosomal protein S4 signature. / Trp-Asp (WD) repeats signature. / Ribosomal protein S7e signature. / Ribosomal protein S28e signature. / Ribosomal protein L7Ae signature. / Ribosomal protein S3Ae signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / Brix domain profile. / S4 RNA-binding domain profile. / Nop domain profile. / Bms1-type guanine nucleotide-binding (G) domain profile. / U3 small nucleolar RNA-associated protein 10 / Sas10 C-terminal domain / Ribosomal protein S8e / Utp21 specific WD40 associated putative domain / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Function and homology information
Specimen sourceChaetomium thermophilum (fungus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 3.2 Å resolution
AuthorsCheng, J. / Kellner, N. / Berninghausen, O. / Hurt, E. / Beckmann, R.
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2017
Title: 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.
Authors: Jingdong Cheng / Nikola Kellner / Otto Berninghausen / Ed Hurt / Roland Beckmann
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Aug 12, 2017 / Release: Oct 11, 2017
RevisionDateData content typeGroupCategoryItemProviderType
1.0Oct 11, 2017Structure modelrepositoryInitial release
1.1Nov 15, 2017Structure modelDatabase referencescitation_citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title

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Structure visualization

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Assembly

Deposited unit
A: Periodic tryptophan protein 2-like protein
B: Utp2
C: Utp3
D: Utp4
E: Utp6
F: Utp7
G: Utp10
H: U3 small nucleolar RNA-associated protein 11
I: Utp12
J: Utp13
K: Utp14
L: Utp15
M: Utp17
N: Utp18
O: Putative U3 snoRNP protein
P: Utp24
Q: Utp30
R: Nop1
S: Nop1
T: Putative nucleolar protein
U: Nop58
V: Snu13
W: Snu13
X: Rrp9
Y: RNA 3'-terminal phosphate cyclase-like protein
Z: Bms1
a: Imp3
b: Putative U3 small nucleolar ribonucleoprotein
c: Putative U3 small nucleolar ribonucleoprotein protein
d: Sof1
e: Emg1
f: Emg1
g: KRR1 small subunit processome component
h: Pre-rRNA-processing protein PNO1
i: RNA cytidine acetyltransferase
j: RNA cytidine acetyltransferase
k: Fcf2
l: 40S ribosomal protein S1
m: 40S ribosomal protein S4
n: 40S ribosomal protein s5-like protein
o: 40S ribosomal protein S6
p: 40S ribosomal protein S7-like protein
q: 40S ribosomal protein S8
r: 40S ribosomal protein s9-like protein
s: 40S ribosomal protein S13-like protein
t: 40S ribosomal protein S14-like protein
u: 40S ribosomal protein S16-like protein
v: 40S ribosomal protein S11-like protein
w: 40S ribosomal protein S22-like protein
x: 40S ribosomal protein s23-like protein
y: 40S ribosomal protein S24
z: 40S ribosomal protein S28-like protein
0: Faf1
1: 35S rRNA
2: U3 snoRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,735,32556
Polyers3,735,26055
Non-polymers651
Water0
1


  • idetical with deposited unit
  • defined by author
  • Evidence: immunoprecipitation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein/peptide , 32 types, 36 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZadef...

#1: Protein/peptide Periodic tryptophan protein 2-like protein / Utp1


Mass: 100817.508 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SF93
#2: Protein/peptide Utp2


Mass: 103960.703 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SDW0
#3: Protein/peptide Utp3


Mass: 73214.633 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S4H3
#4: Protein/peptide Utp4


Mass: 97696.523 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SCT7
#5: Protein/peptide Utp6 /


Mass: 46924.773 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S387
#6: Protein/peptide Utp7


Mass: 63629.379 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RZ99
#7: Protein/peptide Utp10


Mass: 198508.438 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S5L1
#8: Protein/peptide U3 small nucleolar RNA-associated protein 11 / U3 snoRNA-associated protein 11 / Utp11


Mass: 31379.650 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SF32
#9: Protein/peptide Utp12


Mass: 107573.039 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RZL9
#10: Protein/peptide Utp13


Mass: 100430.352 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SG95
#11: Protein/peptide Utp14


Mass: 104741.391 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S8Y4
#12: Protein/peptide Utp15 /


Mass: 60524.145 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SEI5
#13: Protein/peptide Utp17


Mass: 105039.695 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SCS8
#14: Protein/peptide Utp18 /


Mass: 69055.141 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SBN9
#15: Protein/peptide Putative U3 snoRNP protein / Utp21


Mass: 115122.008 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S872
#16: Protein/peptide Utp24


Mass: 22267.188 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SE30
#17: Protein/peptide Utp30


Mass: 43700.527 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S7X0
#18: Protein/peptide Nop1


Mass: 32960.844 Da / Num. of mol.: 2
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SGY2
#19: Protein/peptide Putative nucleolar protein / Nucleolus / Nop56


Mass: 57830.699 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S2U4
#20: Protein/peptide Nop58 /


Mass: 64292.113 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RYF6
#21: Protein/peptide Snu13


Mass: 13831.854 Da / Num. of mol.: 2
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SGM0
#22: Protein/peptide Rrp9 /


Mass: 69610.438 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SG51
#23: Protein/peptide RNA 3'-terminal phosphate cyclase-like protein / Rcl1


Mass: 43399.766 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S0B1
#24: Protein/peptide Bms1


Mass: 134172.250 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S445
#25: Protein/peptide Imp3 /


Mass: 21802.396 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SDL4
#28: Protein/peptide Sof1


Mass: 50869.828 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RY94
#29: Protein/peptide Emg1 /


Mass: 27936.164 Da / Num. of mol.: 2
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SAY9
#30: Protein/peptide KRR1 small subunit processome component / / KRR-R motif-containing protein 1


Mass: 37500.777 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S3V7
#31: Protein/peptide Pre-rRNA-processing protein PNO1 / Dim2


Mass: 28715.047 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S3T2
#32: Protein/peptide RNA cytidine acetyltransferase / 18S rRNA cytosine acetyltransferase / Kre33


Mass: 119795.125 Da / Num. of mol.: 2
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S273, Transferring groups other than amino-acyl groups
#33: Protein/peptide Fcf2


Mass: 22569.129 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S4E3
#49: Protein/peptide Faf1 /


Mass: 34576.586 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S5F1

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Putative U3 small nucleolar ... , 2 types, 2 molecules bc

#26: Protein/peptide Putative U3 small nucleolar ribonucleoprotein / Imp4


Mass: 34216.266 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SE90
#27: Protein/peptide Putative U3 small nucleolar ribonucleoprotein protein / Mpp10


Mass: 86082.141 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S9I7

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40S ribosomal protein ... , 15 types, 15 molecules lmnopqrstuvwxyz

#34: Protein/peptide 40S ribosomal protein S1 / / eS1


Mass: 29245.158 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S7T8
#35: Protein/peptide 40S ribosomal protein S4 / / eS4


Mass: 29800.764 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S1A6
#36: Protein/peptide 40S ribosomal protein s5-like protein / Ribosome / uS7


Mass: 23679.225 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S1Z0
#37: Protein/peptide 40S ribosomal protein S6 / / eS6


Mass: 27490.139 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RY43
#38: Protein/peptide 40S ribosomal protein S7-like protein / Ribosome / eS7


Mass: 23084.650 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S8C4
#39: Protein/peptide 40S ribosomal protein S8 / / eS8


Mass: 23102.416 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RY45
#40: Protein/peptide 40S ribosomal protein s9-like protein / Ribosome / uS4


Mass: 22029.836 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S0Z4
#41: Protein/peptide 40S ribosomal protein S13-like protein / Ribosome / uS15


Mass: 16912.891 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RZM9
#42: Protein/peptide 40S ribosomal protein S14-like protein / Ribosome


Mass: 16071.464 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SFL1
#43: Protein/peptide 40S ribosomal protein S16-like protein / Ribosome / uS9


Mass: 15962.771 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SBR7
#44: Protein/peptide 40S ribosomal protein S11-like protein / Ribosome / eS17


Mass: 18698.182 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SF00
#45: Protein/peptide 40S ribosomal protein S22-like protein / Ribosome / uS8


Mass: 14905.471 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0SHI0
#46: Protein/peptide 40S ribosomal protein s23-like protein / Ribosome / uS12


Mass: 15934.653 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0RY17
#47: Protein/peptide 40S ribosomal protein S24 / / eS24


Mass: 15535.286 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: P0CU28
#48: Protein/peptide 40S ribosomal protein S28-like protein / Ribosome / eS28


Mass: 7741.980 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
References: UniProt: G0S9M9

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RNA chain , 2 types, 2 molecules 12

#50: RNA chain 35S rRNA


Mass: 827801.812 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
#51: RNA chain U3 snoRNA


Mass: 87992.648 Da / Num. of mol.: 1
Source: (natural) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)

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Non-polymers , 1 types, 1 molecules

#52: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Formula: Zn / Zinc

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The 90S Pre-ribosomne / Type: COMPLEX
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51
Source: NATURAL
Source (natural)Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 2.4 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.11.1_2575: / Classification: refinement
EM software
IDNameVersionCategory
1Gautomatch0.50particle selection
4CTFFIND4CTF correction
7UCSF Chimeramodel fitting
8Cootmodel fitting
10RELION2.0initial Euler assignment
11RELION2.0final Euler assignment
12RELION2.0classification
13RELION2.03D reconstruction
14PHENIXmodel refinement
15REFMACmodel refinement
CTF correctionType: NONE
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 231121 / Symmetry type: POINT
Atomic model buildingRef protocol: AB INITIO MODEL
Refine LS restraints
Refine IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.007140858
ELECTRON MICROSCOPYf_angle_d1.137198388
ELECTRON MICROSCOPYf_dihedral_angle_d14.15181369
ELECTRON MICROSCOPYf_chiral_restr0.05924284
ELECTRON MICROSCOPYf_plane_restr0.00819361

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