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- PDB-5fl8: CRYO-EM STRUCTURE OF THE RIX1-REA1 PRE-60S PARTICLE -

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Entry
Database: PDB / ID: 5fl8
TitleCRYO-EM STRUCTURE OF THE RIX1-REA1 PRE-60S PARTICLE
Components
  • (60S ribosomal protein ...) x 36
  • (Ribosome assembly ...) x 2
  • 25S ribosomal RNA
  • 5.8S ribosomal RNA
  • 5S ribosomal RNA
  • ARX1
  • Eukaryotic translation initiation factor 6
  • Midasin
  • Nucleolar GTP-binding protein 1
  • Protein SDA1
  • Ribosome biogenesis protein RLP24
  • Rpl9ap
KeywordsRIBOSOME
Function / homology50S ribosomal protein L30e-like / Ribosomal protein L14 superfamily / Ribosomal protein L5, N-terminal / Ribosome assembly factor Mrt4 / Ribosomal protein L19, eukaryotic / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L19/L19e superfamily / Ribosomal protein L29/L35 superfamily / Ribosomal L18e/L15P superfamily / WD40-repeat-containing domain superfamily ...50S ribosomal protein L30e-like / Ribosomal protein L14 superfamily / Ribosomal protein L5, N-terminal / Ribosome assembly factor Mrt4 / Ribosomal protein L19, eukaryotic / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L19/L19e superfamily / Ribosomal protein L29/L35 superfamily / Ribosomal L18e/L15P superfamily / WD40-repeat-containing domain superfamily / Ribosomal protein L32e superfamily / Ribosomal protein L22/L17 superfamily / von Willebrand factor A-like domain superfamily / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L13 superfamily / OBG-type guanine nucleotide-binding (G) domain / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L21 superfamily / Ribosomal protein L36e domain superfamily / Ribosomal protein L38e superfamily / Ribosomal protein L7A/L8 superfamily / Ribosomal protein L22e superfamily / Ribosomal protein L34Ae superfamily / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L27e superfamily / Ribosomal protein L35A superfamily / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L22p/L17e / Ribosomal protein L14p/L23e / Ribosomal protein L23 / Ribosomal protein L5, C-terminal / Ribosomal protein L15 / Ribosomal protein L3 / Ribosomal protein L30, central domain, archaeal type / G-protein beta WD-40 repeat / Ribosomal protein L11, C-terminal / Ribosomal protein L11, N-terminal / Ribosomal protein L11, conserved site / Ribosomal protein L15e, conserved site / Ribosomal protein L5, conserved site / Ribosomal protein L18e/L15P / Ribosomal protein L18e, conserved site / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein L5 domain superfamily / Ribosomal protein L30e, conserved site / Ribosomal protein L13, conserved site / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Nucleolar GTP-binding protein 1 / Ribosomal protein L1/ribosomal biogenesis protein / P-loop containing nucleoside triphosphate hydrolase / Sda1 / 60S ribosomal protein L4, C-terminal domain / Sigma-54 interaction domain, ATP-binding site 1 / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / Ribosomal protein L15e core domain superfamily / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L1-like / Ribosomal protein L1, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L39e domain superfamily / Ribosomal protein L31e domain superfamily / Ribosomal protein L4 domain superfamily / Ribosomal protein L5 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L31e, conserved site / Ribosomal protein L5 signature. / Nucleolar GTP-binding protein 1 (NOG1) / AAA domain (dynein-related subfamily) / Ribosomal L30 N-terminal domain / NLE (NUC135) domain / NOGCT (NUC087) domain / NUC130/3NT domain / Ribosomal L18 C-terminal region / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins L26 eukaryotic, L24P archaeal / Ribosomal protein 60S L18 and 50S L18e / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L14 signature. / Ribosomal protein L23 signature. / Ribosomal protein L39e signature. / Ribosomal protein L11 signature. / Ribosomal Proteins L2, C-terminal domain / Ribosomal protein L22 signature. / Ribosomal protein L2 signature. / Ribosomal protein L3 signature. / Ribosomal protein L15 signature. / Ribosomal protein L19e signature. / Ribosomal protein L29 signature. / Ribosomal protein L32e signature. / Ribosomal protein L30 signature. / Trp-Asp (WD) repeats signature. / Ribosomal protein L6 signature 2. / Ribosomal protein L30e signature 1. / Ribosomal protein L13 signature. / Ribosomal protein L1e signature.
Function and homology information
Specimen sourceSaccharomyces cerevisiae (baker's yeast)
SACCHAROMYCES CEREVISIAE (baker's yeast)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 9.5 Å resolution
AuthorsBARRIO-GARCIA, C. / THOMS, M. / FLEMMING, D. / KATER, L. / BERNINGHAUSEN, O. / BASSLER, J. / BECKMANN, R. / HURT, E.
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2016
Title: Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling.
Authors: Clara Barrio-Garcia / Matthias Thoms / Dirk Flemming / Lukas Kater / Otto Berninghausen / Jochen Baßler / Roland Beckmann / Ed Hurt
Abstract: Ribosome synthesis is catalyzed by ∼200 assembly factors, which facilitate efficient production of mature ribosomes. Here, we determined the cryo-EM structure of a Saccharomyces cerevisiae ...Ribosome synthesis is catalyzed by ∼200 assembly factors, which facilitate efficient production of mature ribosomes. Here, we determined the cryo-EM structure of a Saccharomyces cerevisiae nucleoplasmic pre-60S particle containing the dynein-related 550-kDa Rea1 AAA(+) ATPase and the Rix1 subcomplex. This particle differs from its preceding state, the early Arx1 particle, by two massive structural rearrangements: an ∼180° rotation of the 5S ribonucleoprotein complex and the central protuberance (CP) rRNA helices, and the removal of the 'foot' structure from the 3' end of the 5.8S rRNA. Progression from the Arx1 to the Rix1 particle was blocked by mutational perturbation of the Rix1-Rea1 interaction but not by a dominant-lethal Rea1 AAA(+) ATPase-ring mutant. After remodeling, the Rix1 subcomplex and Rea1 become suitably positioned to sense correct structural maturation of the CP, which allows unidirectional progression toward mature ribosomes.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Oct 22, 2015 / Release: Dec 2, 2015
RevisionDateData content typeGroupCategoryItemProviderType
1.0Dec 2, 2015Structure modelrepositoryInitial release
1.1Dec 9, 2015Structure modelDatabase references / Other
1.2Dec 23, 2015Structure modelOther
1.3Feb 3, 2016Structure modelDatabase references / Other / Source and taxonomy
1.4Apr 19, 2017Structure modelOther
1.5Aug 30, 2017Structure modelData collection / Derived calculationsem_software / struct_conn_em_software.image_processing_id

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Assembly

Deposited unit
A: 60S ribosomal protein L2-A
B: 60S ribosomal protein L3
C: 60S ribosomal protein L4-A
D: 60S ribosomal protein L5
E: 60S ribosomal protein L6-A
F: 60S ribosomal protein L7-A
G: 60S ribosomal protein L8-A
H: Rpl9ap
I: 60S ribosomal protein L1-B
J: 60S ribosomal protein L11-A
K: 60S ribosomal protein L12-A
L: 60S ribosomal protein L13-A
M: 60S ribosomal protein L14-A
N: 60S ribosomal protein L15-A
O: 60S ribosomal protein L16-A
P: 60S ribosomal protein L17-A
Q: 60S ribosomal protein L18-A
R: 60S ribosomal protein L19-A
S: 60S ribosomal protein L20-A
T: 60S ribosomal protein L21-A
U: 60S ribosomal protein L22-A
V: 60S ribosomal protein L23-A
X: 60S ribosomal protein L25
Y: 60S ribosomal protein L26-A
Z: 60S ribosomal protein L27-A
a: 60S ribosomal protein L28
c: 60S ribosomal protein L30
d: 60S ribosomal protein L31-A
e: 60S ribosomal protein L32
f: 60S ribosomal protein L33-A
g: 60S ribosomal protein L34-A
h: 60S ribosomal protein L35-A
i: 60S ribosomal protein L36-A
j: 60S ribosomal protein L37-A
k: 60S ribosomal protein L38
l: 60S ribosomal protein L39
m: Eukaryotic translation initiation factor 6
n: Ribosome assembly factor MRT4
o: Nucleolar GTP-binding protein 1
p: 60S ribosomal protein L43-A
q: Ribosome assembly protein 4
r: Protein SDA1
s: Midasin
t: Ribosome biogenesis protein RLP24
u: ARX1
x: 25S ribosomal RNA
y: 5.8S ribosomal RNA
z: 5S ribosomal RNA


Theoretical massNumber of molelcules
Total (without water)2,592,65048
Polyers2,592,65048
Non-polymers00
Water0
1


  • idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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60S ribosomal protein ... , 36 types, 36 molecules ABCDEFGIJKLMNOPQRSTUVXYZacdefg...

#1: Protein/peptide 60S ribosomal protein L2-A / / L5 / RP8 / YL6


Mass: 27463.574 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX45
#2: Protein/peptide 60S ribosomal protein L3 / / Maintenance of killer protein 8 / RP1 / Trichodermin resistance protein / YL1


Mass: 43850.793 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P14126
#3: Protein/peptide 60S ribosomal protein L4-A / / L2 / RP2 / YL2


Mass: 39159.125 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P10664
#4: Protein/peptide 60S ribosomal protein L5 / / L1 / L1a / Ribosomal 5S RNA-binding protein / YL3


Mass: 33764.828 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P26321
#5: Protein/peptide 60S ribosomal protein L6-A / / L17 / RP18 / YL16


Mass: 20000.564 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q02326
#6: Protein/peptide 60S ribosomal protein L7-A / / L6 / RP11 / YL8


Mass: 27686.281 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05737
#7: Protein/peptide 60S ribosomal protein L8-A / / L4 / L4-2 / L7a-1 / Maintenance of killer protein 7 / RP6 / YL5


Mass: 28175.820 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P17076
#9: Protein/peptide 60S ribosomal protein L1-B / / L10a


Mass: 24524.799 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX44
#10: Protein/peptide 60S ribosomal protein L11-A / / L16 / RP39 / YL22


Mass: 19755.691 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0C0W9
#11: Protein/peptide 60S ribosomal protein L12-A / / L15 / YL23


Mass: 17850.621 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX53
#12: Protein/peptide 60S ribosomal protein L13-A /


Mass: 22604.164 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q12690
#13: Protein/peptide 60S ribosomal protein L14-A /


Mass: 15195.066 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P36105
#14: Protein/peptide 60S ribosomal protein L15-A / / L13 / RP15R / YL10 / YP18


Mass: 24482.357 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05748
#15: Protein/peptide 60S ribosomal protein L16-A / / L13a / L21 / RP22 / YL15


Mass: 22247.227 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P26784
#16: Protein/peptide 60S ribosomal protein L17-A / / L20A / YL17


Mass: 20589.518 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05740
#17: Protein/peptide 60S ribosomal protein L18-A / / RP28


Mass: 20609.252 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX49
#18: Protein/peptide 60S ribosomal protein L19-A / / L23 / RP15L / RP33 / YL14


Mass: 21762.316 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX82
#19: Protein/peptide 60S ribosomal protein L20-A / / L18a


Mass: 20478.852 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX23
#20: Protein/peptide 60S ribosomal protein L21-A /


Mass: 18279.266 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q02753
#21: Protein/peptide 60S ribosomal protein L22-A / / L1c / RP4 / YL31


Mass: 13711.359 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05749
#22: Protein/peptide 60S ribosomal protein L23-A / / L17a / YL32


Mass: 14493.950 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX41
#23: Protein/peptide 60S ribosomal protein L25 / / RP16L / YL25 / YP42'


Mass: 15787.612 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P04456
#24: Protein/peptide 60S ribosomal protein L26-A / / L33 / YL33


Mass: 14265.784 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05743
#25: Protein/peptide 60S ribosomal protein L27-A /


Mass: 15568.360 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0C2H6
#26: Protein/peptide 60S ribosomal protein L28 / / L27a / L29 / RP44 / RP62 / YL24


Mass: 16761.666 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P02406
#27: Protein/peptide 60S ribosomal protein L30 / / L32 / RP73 / YL38


Mass: 11430.364 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P14120
#28: Protein/peptide 60S ribosomal protein L31-A / / L34 / YL28


Mass: 12980.158 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0C2H8
#29: Protein/peptide 60S ribosomal protein L32 /


Mass: 14809.441 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P38061
#30: Protein/peptide 60S ribosomal protein L33-A / / L37 / RP47 / YL37


Mass: 12177.130 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05744
#31: Protein/peptide 60S ribosomal protein L34-A /


Mass: 13673.196 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P87262
#32: Protein/peptide 60S ribosomal protein L35-A /


Mass: 13942.640 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX84
#33: Protein/peptide 60S ribosomal protein L36-A / / L39 / YL39


Mass: 11151.259 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P05745
#34: Protein/peptide 60S ribosomal protein L37-A / / L43 / YL35 / YP55


Mass: 9877.395 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P49166
#35: Protein/peptide 60S ribosomal protein L38 /


Mass: 8845.561 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P49167
#36: Protein/peptide 60S ribosomal protein L39 / / L46 / YL40


Mass: 6358.640 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P04650
#40: Protein/peptide 60S ribosomal protein L43-A / / L37a / YL35


Mass: 10112.952 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P0CX25

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Protein/peptide , 7 types, 7 molecules Hmorstu

#8: Protein/peptide Rpl9ap


Mass: 21605.061 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:N1P1M8
#37: Protein/peptide Eukaryotic translation initiation factor 6 / eIF-6


Mass: 26476.605 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q12522
#39: Protein/peptide Nucleolar GTP-binding protein 1


Mass: 74531.227 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q02892
#42: Protein/peptide Protein SDA1 / Severe depolymerization of actin protein 1


Mass: 86755.578 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P53313
#43: Protein/peptide Midasin / MIDAS-containing protein / Ribosome export/assembly protein 1


Mass: 560352.875 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q12019
#44: Protein/peptide Ribosome biogenesis protein RLP24 / / Ribosomal protein L24-like


Mass: 24027.650 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:Q07915
#45: Protein/peptide ARX1


Mass: 41870.898 Da / Num. of mol.: 1 / Source: (natural) SACCHAROMYCES CEREVISIAE (baker's yeast)

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Ribosome assembly ... , 2 types, 2 molecules nq

#38: Protein/peptide Ribosome assembly factor MRT4 / mRNA turnover protein 4


Mass: 27098.012 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P33201
#41: Protein/peptide Ribosome assembly protein 4 / Notchless protein homolog 1 / Ribosome biogenesis factor RSA4


Mass: 57106.781 Da / Num. of mol.: 1
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt:P25382

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RNA chain , 3 types, 3 molecules xyz

#46: RNA chain 25S ribosomal RNA /


Mass: 898763.562 Da / Num. of mol.: 1 / Source: (natural) SACCHAROMYCES CEREVISIAE (baker's yeast)
#47: RNA chain 5.8S ribosomal RNA


Mass: 50682.922 Da / Num. of mol.: 1 / Source: (natural) SACCHAROMYCES CEREVISIAE (baker's yeast)
#48: RNA chain 5S ribosomal RNA /


Mass: 38951.105 Da / Num. of mol.: 1 / Source: (natural) Saccharomyces cerevisiae (baker's yeast) / References: GenBank:834774822

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: RIX1-REA1 PARTICLE / Type: COMPLEX
Buffer solutionName: 50 MM TRIS, 100 MM NACL, 1.5 MM OR 5 MM MGCL2 / Details: 50 MM TRIS, 100 MM NACL, 1.5 MM OR 5 MM MGCL2 / pH: 7.5
SpecimenConc.: 2.06 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: CARBON
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Details: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS / Date: Jun 12, 2014
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 75000 / Nominal defocus max: 4200 nm / Nominal defocus min: 1100 nm / Cs: 2.7 mm
Image recordingElectron dose: 20 e/Å2 / Film or detector model: GENERIC TVIPS
Image scansNumber digital images: 2659

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Processing

EM software
IDNameCategory
1Cootmodel fitting
2MDFFmodel fitting
3UCSF Chimeramodel fitting
4Signatureparticle selection
5SPIDER3D reconstruction
CTF correctionDetails: SUBVOLUMES
SymmetryPoint symmetry: C1
3D reconstructionResolution: 9.5 Å / Number of particles: 15749 / Nominal pixel size: 2.06 / Actual pixel size: 2.06
Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3199
Symmetry type: POINT

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