EMDB-3847: Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum

Basic information

• Entry: Database: EMDB / ID: EMD-3847
• Title: Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum

Sample

• Complex: The 90S Pre-ribosomne
  • Protein or peptide: x 49 types
  • RNA: x 2 types
• Ligand: x 1 types

• Keywords: Ribosome biogenesis / RIBOSOME

Function / homology

Function:

box H/ACA snoRNP complex / snRNA pseudouridine synthesis / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / Noc4p-Nop14p complex / tRNA acetylation / t-UTP complex / Mpp10 complex / Pwp2p-containing subcomplex of 90S preribosome / rRNA (pseudouridine) methyltransferase activity / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D methylation guide snoRNP complex / positive regulation of rRNA processing / rRNA primary transcript binding / rRNA base methylation / sno(s)RNA-containing ribonucleoprotein complex / histone H2AQ104 methyltransferase activity / U3 snoRNA binding / snoRNA binding / positive regulation of transcription by RNA polymerase I / 90S preribosome / RNA nuclease activity / RNA processing / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA endonuclease activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / methyltransferase activity / maturation of SSU-rRNA / small-subunit processome / mRNA splicing, via spliceosome / rRNA processing / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / small ribosomal subunit rRNA binding / methylation / tRNA binding / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / GTP binding / nucleolus / RNA binding / ATP binding / nucleus / cytoplasm

Domain/homology:

WD repeat-containing protein 75 first beta-propeller / : / Ribosomal RNA assembly KRR1 / : / : / : / KRR1 small subunit processome component, second KH domain / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10/TcmA, helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / GNAT acetyltransferase 2 / Nucleolar protein 14 / Nop14-like family / U3 small nucleolar RNA-associated protein 6 / U3 small nucleolar RNA-associated protein 4 / : / U3 small nucleolar RNA-associated protein 6 / Small-subunit processome, Utp11 / Sof1-like protein / BING4, C-terminal domain / Fcf2 pre-rRNA processing, C-terminal / rRNA-processing protein Fcf1, PIN domain / Fcf2/DNTTIP2 / WD repeat-containing protein WDR46/Utp7 / : / Utp11 protein / Sof1-like domain / BING4CT (NUC141) domain / Fcf2 pre-rRNA processing / BING4CT (NUC141) domain / : / Small-subunit processome, Utp14 / U3 small nucleolar RNA-associated protein 15, C-terminal / Utp14 protein / UTP15 C terminal / rRNA-processing protein Fcf1/Utp23 / Sas10 C-terminal domain / Fcf1 / Sas10 C-terminal domain / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / : / BP28, C-terminal domain / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosome biogenesis protein Bms1, N-terminal / U3 small nucleolar RNA-associated protein 10 / : / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / HEATR1-like, HEAT repeats / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal RNA-processing protein Rrp9-like / RNA 3'-terminal phosphate cyclase / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Nucleolar protein Nop56/Nop58 / Krr1, KH1 domain / Krr1 KH1 domain / rRNA 2'-O-methyltransferase fibrillarin-like / Fibrillarin, conserved site / Fibrillarin / Fibrillarin signature. / Fibrillarin / : / : / Eukaryotic type KH-domain (KH-domain type I) (327.11.2) / : / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / U3 snoRNP protein/Ribosome production factor 1 / Large family of predicted nucleotide-binding domains / Quinoprotein amine dehydrogenase, beta chain-like / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal

Component:

40S ribosomal protein s23-like protein / 40S ribosomal protein S6 / 40S ribosomal protein S8 / DDB1- and CUL4-associated factor 13 / Nucleolar protein 58 / BING4 C-terminal domain-containing protein / Small-subunit processome Utp12 domain-containing protein / 40S ribosomal protein S13-like protein / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein / 40S ribosomal protein S4 / 40S ribosomal protein s5-like protein / RNA cytidine acetyltransferase / Nucleolar protein 56 / U3 snoRNP protein / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 pre-rRNA processing C-terminal domain-containing protein / Sas10 C-terminal domain-containing protein / Uncharacterized protein / U3 small nucleolar RNA-associated protein 10 / Small ribosomal subunit protein eS1 / Uncharacterized protein / Putative U3 snoRNP protein / 40S ribosomal protein S7 / Uncharacterized protein / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Nucleolar essential protein 1 / Uncharacterized protein / 40S ribosomal protein S16-like protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Nucleolar complex protein 14 / PIN domain-containing protein / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 15 C-terminal domain-containing protein / 40S ribosomal protein S11-like protein / U3 small nucleolar RNA-associated protein 11 / Periodic tryptophan protein 2-like protein / 40S ribosomal protein S14-like protein / Ribosomal RNA-processing protein / U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein / H/ACA ribonucleoprotein complex subunit 2 / rRNA 2'-O-methyltransferase fibrillarin / 40S ribosomal protein S22-like protein / Small ribosomal subunit protein eS24

• Biological species: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Method: single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Cheng J / Kellner N
• Citation: Journal: Nat Struct Mol Biol / Year: 2017; Title: 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.; Authors: Jingdong Cheng / Nikola Kellner / Otto Berninghausen / Ed Hurt / Roland Beckmann / ; Abstract: The 40S small ribosomal subunit is cotranscriptionally assembled in the nucleolus as part of a large chaperone complex called the 90S preribosome or small-subunit processome. Here, we present the 3.2-Å-resolution structure of the Chaetomium thermophilum 90S preribosome, which allowed us to build atomic structures for 34 assembly factors, including the Mpp10 complex, Bms1, Utp14 and Utp18, and the complete U3 small nucleolar ribonucleoprotein. Moreover, we visualized the U3 RNA heteroduplexes with a 5' external transcribed spacer (5' ETS) and pre-18S RNA, and their stabilization by 90S factors. Overall, the structure explains how a highly intertwined network of assembly factors and pre-rRNA guide the sequential, independent folding of the individual pre-40S domains while the RNA regions forming the 40S active sites are kept immature. Finally, by identifying the unprocessed A1 cleavage site and the nearby Utp24 endonuclease, we suggest a proofreading model for regulated 5'-ETS separation and 90S-pre-40S transition.

History

Header (metadata) release	Jul 19, 2017	-
Deposition	Aug 12, 2017	-
Map release	Oct 11, 2017	-
Update	May 15, 2024	-
Current status	May 15, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_3847.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.084 Å

Density

Contour Level	By AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum	-0.3247096 - 0.5959159
Average (Standard dev.)	0.00035213277 (±0.0108130425)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 520.32 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.084	1.084	1.084
M x/y/z	480	480	480
origin x/y/z	0.000	0.000	0.000
length x/y/z	520.320	520.320	520.320
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	480	480	480
D min/max/mean	-0.325	0.596	0.000

Supplemental data

Sample components

Entire : The 90S Pre-ribosomne

• Entire: Name: The 90S Pre-ribosomne

Components

• Complex: The 90S Pre-ribosomne
  • Protein or peptide: Periodic tryptophan protein 2-like protein
  • Protein or peptide: Utp2
  • Protein or peptide: Utp3
  • Protein or peptide: Utp4
  • Protein or peptide: Utp6
  • Protein or peptide: Utp7
  • Protein or peptide: Utp10
  • Protein or peptide: U3 small nucleolar RNA-associated protein 11
  • Protein or peptide: Utp12
  • Protein or peptide: Utp13
  • Protein or peptide: Utp14
  • Protein or peptide: Utp15
  • Protein or peptide: Utp17
  • Protein or peptide: Utp18
  • Protein or peptide: Putative U3 snoRNP protein
  • Protein or peptide: Utp24
  • Protein or peptide: Utp30
  • Protein or peptide: Nop1
  • Protein or peptide: Putative nucleolar protein
  • Protein or peptide: Nop58
  • Protein or peptide: Snu13
  • Protein or peptide: Rrp9
  • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
  • Protein or peptide: Bms1
  • Protein or peptide: Imp3
  • Protein or peptide: Putative U3 small nucleolar ribonucleoprotein
  • Protein or peptide: Putative U3 small nucleolar ribonucleoprotein protein
  • Protein or peptide: Sof1
  • Protein or peptide: Emg1
  • Protein or peptide: KRR1 small subunit processome component
  • Protein or peptide: Pre-rRNA-processing protein PNO1
  • Protein or peptide: RNA cytidine acetyltransferase
  • Protein or peptide: Fcf2
  • Protein or peptide: 40S ribosomal protein S1
  • Protein or peptide: 40S ribosomal protein S4
  • Protein or peptide: 40S ribosomal protein s5-like protein
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S7-like protein
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein s9-like protein
  • Protein or peptide: 40S ribosomal protein S13-like protein
  • Protein or peptide: 40S ribosomal protein S14-like protein
  • Protein or peptide: 40S ribosomal protein S16-like protein
  • Protein or peptide: 40S ribosomal protein S11-like protein
  • Protein or peptide: 40S ribosomal protein S22-like protein
  • Protein or peptide: 40S ribosomal protein s23-like protein
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S28-like protein
  • Protein or peptide: Faf1
  • RNA: 35S rRNA
  • RNA: U3 snoRNA
• Ligand: ZINC ION

Supramolecule #1: The 90S Pre-ribosomne

• Supramolecule: Name: The 90S Pre-ribosomne / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)

Macromolecule #1: Periodic tryptophan protein 2-like protein

• Macromolecule: Name: Periodic tryptophan protein 2-like protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 100.817508 KDa

Sequence

String:

MKTNFKFSNL LGTVYCRGNL LFSPDGTHLF SPVGNRVTVF NLVENKSYTF PFAHRKNISR IGLTPQGNLL LSIDEDGQAI LTNVPRRVV LYHFSFKSPV TALAFSPSGR HFVVGLKRKI EVWHVPSTPD TNEDGDLEFA PFVRHHTHMQ HFDDVRHLEW S SDSRFFLS ASKDLTARIW SLDTEEGFVP TVLSGHRQGV VGAYFSKDQE TIYTVSKDGA VFEWKYVAKP GHEDDEMVDD ED MQWRIVN KHFFMQNAAT LRCAAYHAES NLLVAGFSNG IFGLYEMPDF NLIHTLSISQ NEIDFVTINK SGEWLAFGAS KLG QLLVWE WQSESYILKQ QGHFDAMNSL VYSPDGQRIV TAADDGKIKV WDVESGFCIV TFTEHTSGVT ACEFAKKGSV LFTA SLDGS VRAWDLIRYR NFRTFTAPER LSFTCMAVDP SGEVIAAGSI DSFDIHIWSV QTGQLLDRLS GHEGPVSSLA FAPDG SVLV SGSWDRTARI WSIFSRTQTS EPLQLQSDVL DVAFRPDSKQ IAISTLDGQL TFWSVSEAQQ VSGVDGRRDV SGGRRI TDR RTAANVAGTK NFNTIRYSMD GTCLLAGGNS KYICLYSTTT MVLLKKFTVS VNLSLSGTQE FLNSKLMTEA GPVGLLD DQ GEASDLEDRI DRSLPGSKRG DPGARKKFPE VRVSGVAFSP TGNSFCAAST EGLLVYSLDN TVQFDPFDLN MEITPAST L AVLEKEKDYL KALVMAFRLN EAGLITRVYQ AIPYTDIGLV VEQFPTVYVP RLLRFVAAQT EQSPHMEFCL LWIRALIDK HGPWLAANRG KVDVELRVVA RAVAKMRDEI RRLADENVYM VDYLLNQAKS KSNNRAVVGD AEEEWGGLDD FGVKALPSSK PETTLDDIM QDDVAESEEE WIGLE

UniProtKB: Periodic tryptophan protein 2-like protein

Macromolecule #2: Utp2

• Macromolecule: Name: Utp2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 103.960703 KDa

Sequence

String:

MAGSQLKRLK ASLREQGLIG PQKSKKQKRQ NANDPKAQEK RLQKAEALKS IREQFNPFQF KTNARGPKFE VTTNKPTDKN GMVIKGRPE LSRARSEEKR RQTILVEMQR RNKVGGIIDR RFGEGDPNLS VEDKMIERYT QEALRAHKKK GMFDLEDDDE A RLTHMGKP LFDDDEAPKE DFSEDDLPSG DESDTTRAER RALKRQRLAE AMEGLEDEAG QPERKKTRKE IYEEIIAKSK MY KAERQAI KEENNELRME VDQELPEVQQ LLYQIKKPET EKKEAPVPII AGKEKTVVDK EYDIRLKQLA MDKRAQPAEP VKT EEEKAA EAAKKLQELE EKRLKRMRGE PVSDDEESES EEDKGKKDKK DNAVDPLNAE ESEDEFGLGK GVKYKPTATE LGLD DEDDF LIDDDLVASG SELEFDSEDF SDVESDEEEG SSESKEDEED DEFIKGILTE AEQQEAVFQQ PKQGDDENGI PYTFP CPQS HEEMLNVVKG IEVTKLPTVV QRIRALHHPK LDAKNKERLG NFSQVLIHHI AYLGDRFQPH WFPTLEQLSR HVHSLA KTF PIEVAKAYRM RIQEMEEHRP LAPTVGDLVI LTAIGTTFPT SDHFHQVCTP AMLAIARYLG QKVPAALSDF AVGIYLS IL ALQYQDFAKR YVPEMMNFLL NTLCALAPER AKSKLGNFPV HEPPAGIRIK DATNTPIRQL NCGDCLRKDE LSPAETSS L QIAILSTATA ILKSAADTWH KLPAFIESFQ PALSVAQHLL TKPNASHLPS SLTSKLNDLA SHLSRLLQLS RLSRRPLEL HHHRPLAIKT YIPKFEDDFD PDKHYDPNRE RAELAKLKAE HKRERKGALR ELRKDAQFIR REQLRIKKEK DEAYEKKFKR IIAEIQNEE GRAANEYARE KAARKRKR

UniProtKB: Nucleolar complex protein 14

Macromolecule #3: Utp3

• Macromolecule: Name: Utp3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 73.214633 KDa

Sequence

String:

MAKKRKATRP AEPQGPKEID PKDARLTVNN YMDVADSEDE YWYKKDRLDI DSDDEPRSKR LKRQDKEDAF LEQSDEEIFD DELSSEEEE EDDEDERRAP TKKDGKKGAA LDDDDDAELL GLGKKKDDDE IRDEGWWGSS KKEYYNADAI ETEQDALEEE K EARRLQAK KLAKMQEADF AFDEAEWLAT KDEKTGGDEE VVTEVLKEVE VTSDMGPEER YNLLQARYPE FDYLVEEFRE LR PLLETLQ KEAEGKPAKS LPVVKAWVLG CYVAALASYF AILTSPTRDG DESKGIMNPS ELRDHEVMQT LMECREAWLK VKS LRPAKG AVSAHGMLSP PEEGEEAGSD VDMLDDHAVQ KPKKAKKLSK EEIKANKKKK EEEAKKAKAV EQSLAELTSL LQTT KKAAV KASVASKAMS TAATSGADSG MDDNRSDFGE EEELDARTAA EKAKRKKSLR FYTSQIVQKA NKRQGAGRDA GGDMD IPYR ERLKDRQARL NAEAERRGKR DSKFGADLGG DSSDDEDAKA RQVRDDEDAY YNEVVQAAAK KRADKQARFE ALAAAR KGD RVVEEETIGP DGKRQITWQI QKNKGLTPNR KKEQRNPRVK KRKKYEEKQK KLRSVKAVYK GGEGPGGYQG ELSGIKT NL VKSVKL

UniProtKB: Sas10 C-terminal domain-containing protein

Macromolecule #4: Utp4

• Macromolecule: Name: Utp4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 97.696523 KDa

Sequence

String:

MDIHRCRFVR YPASAINAVA FTHSALPVVS SSKKYLQKNI QVRLAIGRAN GDIEIWNPLN GGWYQEVIIP GGKDRSVDGL VWVTDPDEE MADGKIIHGK SRLFSIGYTT TITEWDLEKA RAKKHASGQH GEIWCFGVQP LPHKANAAAA QNRKLVAGTV D GNLVLYSI EDGDLKFQKT LTRTPSKKTK FVSIAFQSHN IVIVGCSNST ICAYDVRTGT MLRQMTLGTD LTGGSKNIIV WA VKCLPNG DIVSGDSTGQ VCIWDGKTYT QAQRIQSHTQ DVLCLSVSAD GSKIISGGMD RRTAVYEPMA GQSGRWSKVF HRR YHQHDV KAMASFEGKG MSVVVSGGSD ASPIVLPLRA LGKEFHRTLP HLPQHPTVLS APKARYILSW WENEIRIWHL LNSA QQFLD DPQAPLNLRK NRKFLAQVLI KGASHITSAS ISEDGTLLAA STPTDVKVFH LDPAAAQRNG QLYIKKVNMT GTGLG ATRV QISPDKRWIC WAEEGSKVMI SRVHATESAD GISYTVSVPH KLHRLRRQIP KHILLGGLGS YDRNVSQIAF SADSRM LSV ADLAGYIDTW VLRGPGEGVN GTGGEDSDGE SAASSSDSSD EKSEDIAGER WARNPKAAMI PKLSAAPVVL SFSPTPR DD GDYDLLVVTT LKQLLIFNPL RGMLSEWSRR NTYPKLPEPF RDTRDQVKGI VWQGQRAWFY GVASLFMFDL SQDFSPEK D LVETNGHKQG TKRKRGAHES GAGSKIEKHS LVPQRIRAAS APDGTKWEDI EMVDADDQKS VGVSSGVDDD DDETDGSEL QRLREENREA NSSANAEKEG PSRAKWWHTY QFRPIMGIVP IEGMMEKKLG AVEGIPPLEV ALIERPLSED DLPERYFAEG EWER

UniProtKB: Uncharacterized protein

Macromolecule #5: Utp6

• Macromolecule: Name: Utp6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 46.924773 KDa

Sequence

String:

MAWVADKARF YLERAAPELR EWEEKEIFTK DEIRNLVAKR SDFEHLVLAP GTKPTDFLNY VNWERSLDRL RAKRCARLNI RSVTSHASQ ARTFGIFERA VLKHPGSIEL WLAYLEFAAQ VKATKRWRRI MTRALRLHPM NASLWTLAGR RAAQNGDMQR A RAHFLRGC RFCTREPTLW LEYARCEMDW LARMEAKKQG QGVRKGVNAL EAIKATEGQE EGDIIPIGED TEDDSGDEDG LI LPDPDAE GTDGTKKAAK PVFDAEQTKK LEQSPALSGA IPIAVFDVAR KQPFWGPAAA EKFFDVFAKF GHLSCHERII SHV VTTMQE LFPNHPCTWS VHIRQPLVGV DVLTPAFPKA LRESLARLKA ALQSTTDRKA LATKMVAWMD GILAIEKLDA AIRT VLEHT KRSLEESPS

UniProtKB: U3 snoRNP protein

Macromolecule #6: Utp7

• Macromolecule: Name: Utp7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 63.629379 KDa

Sequence

String:

MDTSEAVDLA PAPAKQGRQA NGAHSIANAR TEFRSKAELD RIRRYKQAQK KYGRGPRVDI KSVRDKKLRR TLTNLENKYK TAALKAKEA EILLENQTGF LEPEGELERT YKVRQDEIVK EVAVEVAQKK FELKLTELGP YTCEYSRNGR DLILAGRKGH V ATMDWREG KLGCELQLGE TVRDARFLHN NQFFAVAQKK YVYIYDHNGV EIHCLRKHVE VSHMEFLPYH FLLATLSISG QL KYQDTST GQIVAEIATK HGTPVSLTQN PYNAILHIGQ QNGTVTLWSP NSTDPLVKLL AHRGPVRSLA VDREGRYMVS TGQ DNKMCI WDIRNFKEAV NSYFTRAPAT SVAISDTGLT AVGWGTHTTI WKGLFNKERP VQVKVDSPYM TWGGQGQVVE RVRW CPFED ILGIGHNEGF SSIIVPGAGE ANYDALEVNP FETKKQRQEG EVKALLNKLQ PEMIALDPNF IGNLDLRSEK QRQAE RDLD QPAQDIVEEL RKKARGRNTA LKKYLRKQRK KNIIDEKRLK AEELYRQMQE KRDGQTKEKQ AEVGPALARF VRKE

UniProtKB: BING4 C-terminal domain-containing protein

Macromolecule #7: Utp10

• Macromolecule: Name: Utp10 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 198.508438 KDa

Sequence

String:

MASSLAQQLA QIAANSRSSF NVKALKASHS KSLIWEPRVA VSQTFAEIYS QCYEGFKELC HLDSRFVPFD ATLFSAQSQE VDRTQMTAE ENAALDKRVD SFLHLVGSRL RLMPAIKAVE WLIRRFRIHE FNTGTLLATF LPYHTIPAFV TLLSILPVQR I PIEYRFLD PYIKSLTPPP RAAIVQQATN RPDLLSAISR YTLDSCRAKQ EYPGLISFWG GIMAEAVNGM IDKMRSGRRA IQ LENDHLL LQQIGPVLSE AMVMKDVPGI QIASYMVVAI LAAKGSLNDN ILTAFMEQLV HGWTVDTLRP GLVCLTMLAQ HRS AKQLSG RVAKAVIKVP DLVSSLRDIS KEHQVDKLAN GLVLAFVDRL AKKGDIRTLP VINSLLLSEL LQEKQAKVAY KALL LAAHK IDDNVDADGN IRKQVGSALV RLSQAEGDVG DAIRTAIQEV DFNIEELELK LGAAIRPKLA IEEAPEPSDE AMTVR PVDQ RPSLDSTFER LSKLQPTATS CLAKDSESLF NDLCSVFLSA AVSESDLERF DATPVLSRPK APSNSFYLSF YLRVWC GPY PTLAKVAALE RVKTRLKEGD CVDKDFQAIF PYCIAALSDP AKKVRRAAAD LVAVLGSAYK SLEKSQQLWA AKDLYGK TG TTSPLDKDAL KALIRSVLIP CLEESVLHED HVVAALVGAL ESSKDSENKN ADKRHLSHSA RLSIFKFLCG HVVETPLL A VKLRLLRSLN QIRRISGTTR TDLLLPLLRW WAGLSANEAA ELAAQESVDV PAIDDAVVDV VVPNHAAGLE AFFQLVKEA IRPGLLQAIF ARIAKMWPSM KSDTKYSTAK TLFELTQDPK LNAEQSDVIT EAVEVLRKVD LTTDILHYFI DSLQDEVRLA TEGPANKRR RVSTTEPGRG VGTQSSPELQ AALSKTTFVL ELVQESTPAN HPELLPSLFT TLSDLHHLSA IVGSELGYLQ N LVLSSLLA MMPTYKDNKD LTIDASVGHG DVLATCIQKS TSPAVINSAL LLVASLARTA PDVVLQSVMP IFTFMGSSVL KQ ADDYSAH VVNQTIKEVI PPLIETFRKR GRNVVASAKD LLASFVTAYE HIPSHRKHNL FISLIQNLGP DDFLFAILAM FVD RYGATD NMITFMTQLI SSFTVETQLQ TLLKFLDLVG DLFKSKPTLS NVLLGGGSGL NGEQDIQKAA TKQLNLLPHL LSNR RLKRE IMQLAERDDM EAGKIRELYA ALLEGILTVA STVKTKKTLH SRCGDALSNL LNLLSIAEFI KSVEALLDRP NIGLR QKVL RALELRVDSE SSVDPKSREA LLAFLPQLTA VIRESDDMNY KHTAVICVDK ISEKYGKKDL DAVAAAATTI AGDYCL GQP SQSLRVMALL CLASLVDVLQ DGIVPVLPVA IPKALSYLEQ SVAEGGRDVE LHNAAYAFMT ALAQHIPYMI TGNYLDR LL ACSNASAAAN LDDEANANRM QCLQFLAKLV DARVLYTGLH QNWASAAKFG FSAISEYLQI LGIALDKHSK TVVVKNVS S LSSIFLSAMD LRRTVAAGDI ASQISAMELD EIETKTHEDA LKMVYKLNDA AFRPIFSKFV EWATTGLPKS DVTGRTYRL YVVFGFLDAF FGSLKSIVTG YASYIVDASV KALKAVDFAV PEERNLWKRV LCTLAKCFEH DQDGFWQAPA HFGAVAPVLV EQFLRAEGQ VTATNVNDVI QDVVPAVVEL AAAVESQEHY KEINTALLKH LRNGSPGVRL AVVKCQQAIT AKLGEDWLHL L PEMLPYIS ELQDDDDEVV ERENRRWIVG IEEKLGESLD SMLQ

UniProtKB: U3 small nucleolar RNA-associated protein 10

Macromolecule #8: U3 small nucleolar RNA-associated protein 11

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 11 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 31.37965 KDa

Sequence

String:

MSSLRHAIQR RAHKERAQPL ERQRLGILEK KKDYRLRARD YKKKQAVLKS LRQKAAERNE DEFYFGMMSR KGPGSALTRG KGFTGTVDG DRGNKALSVE TVRLLKTQDL GYVRTMRNIA AKELKELEER YVLAGGADQP VEEFNSDEDE EESGSKQAKP K KIVFFEGV EERQQALEKQ KADEEMKDYD EEEDGYDFDD EEEMTEKEKE ERRKQLVLEK LARKVKAARK KLKALADAEY EL ELQQAKM AKTATSGGFT KSGRRIKVRE RKR

UniProtKB: U3 small nucleolar RNA-associated protein 11

Macromolecule #9: Utp12

• Macromolecule: Name: Utp12 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 107.573039 KDa

Sequence

String:

MVKSYLKFEP SKSFGVVVSS NSNLVWSSRG KAGAGAGQAI VAANEEVLVW DIKKGELLSR WRDENCKFRV TAIAQSRTDP DVFAVGYED GSIRLWDSKI ATSVVSFNGH KSAITVLAFD KTGVRLASGS KDTDIIVWDL VAEVGQYKLR GHKDQVTGLY F IEPDPVVR TEGEDDHAVM AVDSEPSDGF LLTTGKDSLI KLWDLSSRHC IETHVAQANG ECWALGVSPD FTGCITAGND GE ITVWALD ADALASSAQK VDLSQSVNFL QNRGTLHRSS KERAAEVVFH PLRDYFAVHG VEKSVEIWRI RNEAEIKKTL ARK KKRRRE KLKEKKAEGA DADMDETDDT DIAKAEVSDV FVQHVIVRTT GKVRSVDWAL NPGVKDLQLL VGGTNNLLEL YNIV GKERL KSKGEEPDYN KALAVELPGH RTDIRSVAIS SDDKMLASAA NGSLKIWNIK TQQCIRTFDC GYALCCAFLP GDKVV IVGT KEGELQLYDV ASASLLETVN AHDGHAVWAL QVHPDGRSVV SGGADKTAKF WDFKIVQEPV LGTTRTTHRL KLVQSR ILK VSDDILSLRF SPDARLLAVA LLDSTVKVFF NDTLKLYLNL YGHKLPVLSM DISYDSKLIV TSSADKNIRI WGLDFGD CH KALFGHQDSI LQVAFIPHNS DGNGHYFFSA SKDRTIKYWD ADKFEQIQRI DGHHGEIWAL AVSHSGRFLV SASHDKSI R VWEETDEQIF LEEEREKELE ELYEQTLTTS LEQDPDEQDA NREIAAATKQ TVETLMAGER IAEALELGMT DLNTIREWE EARQINPNIA PPQRNPIFVA LGNIPAETYV LNTLQKIKPA SLHDALLVLP FSTIPSLLTF LNLFAQRELN VPLTCRILFF VLKTHHKQI VASRTMRATL EKVRANLRAA LRRQKDEMGF NIAALKVVSM QLRDKSVREY VDETWEEKEK EKGVRKRAFA S LS

UniProtKB: Small-subunit processome Utp12 domain-containing protein

Macromolecule #10: Utp13

• Macromolecule: Name: Utp13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 100.430352 KDa

Sequence

String:

MATKQPAKTT FEVANVIQPI YTGGSVALEN GARILASTLG ENAILTELNT GKRLAEIQGD GEPISTLTIT PSGSHLIVCS RSLTMRIYS LAISPDYDSV EPTLVRTTKP HATPVVVLAV DRTSTLLATG AADGAIKIWD IIGGYVTHTV SGPSVLVSAL H FFEIAVTA ESQSSNKKPK KGSRKGQNDD ADEIASRFRL AWGTQDGRIR IFDLYKRTTT PVYADPKRKK EAHESNVQCI AY SPEQHAL LSGSRDKTMT LWLWRDGIWQ GTPMLRHELL ESVGFLNEGK WMYSAGTSGV LRIWDTTTHH EITKKQDAKS EGE AILSAV SLPERSLILC AQADFTLVLY RVPSPADVVS SSEGILLEPF RRISGTHDEI LDLTYILPDQ SMMAIATNSE DIRI VSVKD AQAYSEDNAE CRSGSYFGHD VALLKGHEDI VMSLDVDWSG HWIASGSKDN TARLWRVDPA NNSYTCYAVF TGHLE SVGA VALPKVVPPA NSEAFKNPLD HPPAFLISGS QDRFVQKRDI PRQLQKGGKL TSSLRRLAHD KDINALDISP NGKLFA SAS QDKTVKIWDV EKLEVQGILR GHKRGVWTVR FAPLNTPVIQ GEQGSVSGRG VVLTGSGDKT IKLWNLSDYT CIRTFEG HS HNVLKVVWLH ISRDDSITKT KVQFASAGAD TLVKVWDANT GETECTLDNH EDRLWTLAVH SKTNILASAG SDSKVTFW R DTTAETQAAA AQAALKLVEQ EQTLENYIHA GAYRDAIVLA LQLNHPGRLL NLFTNVVTTR NPDPDSLTGL KAVDDVLAK LSDEQIFQLL LRLRDWNTNA RTAPVAQRVL WALFKSHPAN KLSSLSVKGA RGHKSLNEVL DAIKVYTERH YKRIEELVDE SYLVEYTLR EMDALTPQTE ALEAGEDAVI AEA

UniProtKB: U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein

Macromolecule #11: Utp14

• Macromolecule: Name: Utp14 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 104.741391 KDa

Sequence

String:

MPGRQAHGRP LLPSLGSSSG GGKSKKSRSK ARRSAQSKAL DAFALAAEQV PESRSKGVRL RDLDEHPSTS ASRNKRQHED DEEDDFDGF DDDEGMGRKK RARRDSGGDE DGDDVDEDME GGADGEDDSE EWHVGVTGAD EDSEIDSDEA FGESDEERFE G FVFGGSKS HKKGTKKRRN GSEDDAIDLA TALDQYESSE EGEEGEESES EEDDEESEEE DSDEEDDDPS KLDALQSMIA GF AGEDEES GGEGEAKPSG KQKLSLKDLG LVGVKDPDMK RSLRLMKKEE KATKPGSSKK LEVPLAKRQQ DRLMREAAYK KTN ETLDRW IETVKHNRRA EHLVFPLAQN AHDRGLDASE LQPITQKTSG TELEQTILAI MEESGLGPTA KPEKKETSGE AGKQ GMSKE EQKELIRQKR RERELHSREM ARLKRIKKIK SKTWRRIHRK ELLKNEEAAY QAALEAGELD SEEEREALDR RRALE RVGA RHKESKWAKL GKKAGRAVWD EDFRAGLAEM ARRKDELRKR IEGLKNGSDD SSDDSDGDED ASGDEAAERR KLLAEL EKA AAYSDDDEPH SKLFQLKFMQ RGEELRRKEN EELIAQIRRE LDSDYDGSEP DEVEIGRRTF GMGKKDKTEQ QQAQDQL AR MQAAEARKEK AAARVAAAEE TVKQEAREKA EKDEASAPST AGAWSQPKVE AGAWSKPDDE GSSKPRKAPK GRPEEIDV T DLALAGKPAK AKANGQAKSQ VQGKAHASNG QSSSSQKAST SVAITGAEED GNDSDDSDAV HLPMAIRDRA LIERAFAGD DVAAEFEKEK AEIEADDDEK EIDNTLPGWG SWVGEGVSNR EKRRHQGRFV TKVEGIKKEK RKDYKLKDVI INEKRVKKND KYLATGLPH PFESQQQYER SLRLPVGPEW MTKETFQDAT KPRVIIKQGI IAPMSKPMY

UniProtKB: Uncharacterized protein

Macromolecule #12: Utp15

• Macromolecule: Name: Utp15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 60.524145 KDa

Sequence

String:

MAAEVVPLPQ LKLPSGPSPI TAEQRYWRSF KKQKSHTSTA NWPISHISFP ASLGTTALVS SSLVAAAKTN DLFAVTAGPR VEIFSIRKR EPLKTIGRFD SEAHCGEIRP DGRVLVAGED TGRMQVFDVG QGTRAVILKT WHIHKQPVWV TKWSPTELTT L MSCSDDKT VRLWDLPSND PTRLFTGHTD YVRCGAFMPG SANSNLLVSG SYDETVRVWD ARAPGGAVMT FKHADPIEDV LP LPSGTTL LAASGNAISV LDLVAAKPLR LITNHQKTVT SLSLASQGRR VVSGSLDGHV KVFETTSWNV VAGAKYPSPI LSL SVITAG ASHDDRHLAV GMQSGVLSIR TRLSGPAADR ERERERVEAA MAKGPEAIAK LDAAKAKRKR AAVSNKNMDL LGES ADVII PTADPGTHPR GRRPKLKPWQ KAFRQGRYAA AVDDVLNTTA PSYDPVIALT LLTALRHRSA LREALQGRDE LSVIN ILRW AGKYVADPRY RSICVDVAFH LIDLYAEHVG GSAELATQFQ QLLAKVNREV EKAELAIVTG GMVESLMMSV EAQ

UniProtKB: U3 small nucleolar RNA-associated protein 15 C-terminal domain-containing protein

Macromolecule #13: Utp17

• Macromolecule: Name: Utp17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 105.039695 KDa

Sequence

String:

MVSTMETNGD TASLKRKREP KDDPHSLQKK HRHRSRSKPQ DAATADSVAN LNSNSLAVQP IDGNSGPLQL ATTDRLASWK VSKPMGGRM SDIDPIFSQD ERHLIITYNT SIQVYSTEDS LLVRRIALPL TRTNDLDEPS ATHIVSSALS KSNPDYLWVA C SDGRIWHI NWTSGEGVDT PSTIDTKKLL DMAVDAIEVA GKVDDVLLTL NRLTKSSAQI IAYNSKMLAT KTGKLLHTYD ES PQSLRSV AGGRAIVAAA KEALHIGILK TKKLASWEEL AYRFVSFDVP DIISTFDIRP IIRMIKKGVA ELQDIDVAVG GAR GAIYVY SNLLAHLHTE ASGPLRVGTI QPRKYHWHRR AVHSVKWSGD GNYLISGGYE TVLVLWQLDT GRVDFLPHLS AAIE NIVVS PKGSAYALHL DDNSAMVLST AEMKPSMYVS GIQSLVLGDR PSKDALVRRV WRPIDEIASP LVATISPQNP SHMFL CVGN GQQATVGGGA TSTPLVQVFD ISSFQGVAKQ AIARTNPTDV NITSEGVPII EPTATKLAFS HDGKWLASID EWQPPE RDT EAYLTGSKTQ SDACKERREI YLKFWEVGAD QSLELVTRIN DAHYTKQTES IFDLASDPTS ARFATIGNDG MVRFWSP KL RKRDGLMATR PDGQPLRSWS CSRVVPLPVH ERQDDSVEIL KGIPYSGAIT FSEDGSILFA AFGPPSGALV VAIDTQTG T VRDVVSGMFK GDIRAMKSLS SCLIMLSDDL VVYDIVSDEM LASYTLKETS EAAKKLTQLA VNHQSRSFAL AAPIPNLGQ DKLKRGTKSE LLIFNIEDEE PKLVKTFSQV IISVCAVPSS SGFVVVDSAA QVWSITEGAE QAPLLKSLAD LGVDNTSNTE MEDTPDKQL LLEQAEANDD EMHDANYDLD MEDNEDTHAV VVAPQRLAEI FNAAPAFAMP PIEDVFYQVA SLFSTKPVIN A

UniProtKB: Uncharacterized protein

Macromolecule #14: Utp18

• Macromolecule: Name: Utp18 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 69.055141 KDa

Sequence

String:

MPRSNKKKQI EDLPSDSDHF PEFPEEEAFS SLSEEEEAEE RKKPVAEKDA EEEELEKFVL GAKETFREQL FRDDFLAPAN DPTALVKAG DDEEETGHEH LDDSMLFVID TEGDSSIQVP APTKVTAEET GGDAPAWEDS DDERLTISLA GATRLRKLRI S ESEDLVSG IEYARRLRQQ YLRLYPQPDW AKEANGSKRR RRRSLDASSD SLSGSDMEVD SDGESIDAPL PLDSFLRDAN SF KVAAEDS ARSAKRRKLR PETIDIQRTR DIPDTHKAAI SSLAFHPRYP ILLSSSTSSI MYLHKLDASA YPTPNPLLTS VHV KRTDLR RAAFVGPDGG EIIFAGRRRY FHCWNLSSGL VKKVSKIQGH QKEQRTMERF RVSPCGRYMA LVASDKKGGG MLNI INVGT MQWIAQARID GRHGVADFAW WSDGNGLTIA GRDGQVTEWS MITRRTVGIW RDEGSIGGTV MALGGRNGPA ELGGD RWVA IGSNSGILNV YDRNDLIEKP PKKNESNQEE QNSSEKTKEI RIKKYPTPTR VFEQLTTSIS VVAFSPDGQL LAFGSQ HKK DALRLVHLPS CTVYRNWPTE QTPLGRVTAI AFSSKSDVLA VGNDVGRVRL WEIRG

UniProtKB: Uncharacterized protein

Macromolecule #15: Putative U3 snoRNP protein

• Macromolecule: Name: Putative U3 snoRNP protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 115.122008 KDa

Sequence

String:

MPHPEPDFPL TKRQKLDAAP KASSSKRKKP GSAIFAPYRT IGLISPTGVP FTSIPLGKTT FQITTSVGRA LQTYDLKRGL NLVFVTRPQ TPSDITATHA WKERVYAAFG DPRNGEPQGL WVFQRGKKVA ELPLPSDLDQ PIKQILIFGG WIVACALTRI E VWKAATLE HYTTIFPAAS KKGDNELTGG AINMPTFLNK IFVGRKDGWV EIWNVSTGKL IYTLLPPSPD CGAVTCLQPT PA LSLLAIA YSGGPLVIQN VLTDKTVLLL EAGTDDAPVT SISFRTDGLG AGQDGRKDGV MATATSVSGD VTFWDLNKGG RIM GVLRSA HNPPSRHNIV RGGISKIEFL AGQPVIVTSG LDNSLKTWIF DESPFSPVPR ILHQRSGHAA PVRCLHFLPS DFDG AEGGN KWLLSGGKDR SLWGWSLRRD GQSAELSQGA IRKKARKMGL LAGGSHGPTT TLEDLKAPEI TCIASSLNRD GGMGA IPGK QMIWDKGDDK NRISNAELSG NTGWESVVTA HKDDPYARTW FWGRRRAGRW AFKTGDGEPV STVAISSCGT FALVGS TGG SIDMFNLQSG RHRQRFPSRL TPAQLRQLKL QQLRRLDEAN KLAHRSQQKT FAPGTGRHTN AVTGIVVDPL NRHVVSC SL DGKVKFWDFI TGNLVDEIDW APMTKIIGCR YHPGNDLIAF ACDDRSIRVV DIETKNTIRE FWGCRGDIND FCFSPDGR W IVAASQDSII RVWDLPTAHL IDAFRLEQPC TALAFSHTGE YLAGAMEGSL GVQIWTNRTL FRHVPTRQIS ESEIADVAA PTTSGEGGQG LIEAALEAEE EQAEDDGVMA PIIDQLSADM MTLSLVPRSR WQTLLHIDII KARNKPKEPP KAPEKAPFFL PPVGQNGIS SLIPQEDAKA KKEKAAANGA SRITKLDLTR QEQTFTSKLL VGGAKGDYTD FIEHLKALPP AAADLELRSL S IGNGDEAT NELLHFIRAL TSRLVARRDY ELTQAWMTVF LRLHFDLIME NEELLQALGE WREHQARERD RLSELVGYCG GV VSFLRSP RT

UniProtKB: Putative U3 snoRNP protein

Macromolecule #16: Utp24

• Macromolecule: Name: Utp24 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 22.267188 KDa

Sequence

String:

MGVAKRTRKF ATVKRIIGKQ DERRKAEAVK KAEEEKRKKE KQAIREVPQM PSSMFFEHNE ALVPPYNVLV DTNFLSRTVG AKLPLLESA MDCLYASVNI IITSCVMAEL EKLGPRYRVA LMIARDERWQ RLTCDHKGTY ADDCIVDRVQ KHRIYIVATN D RDLKRRIR KIPGVPIMSV QKGKYAIERL PGAPAS

UniProtKB: PIN domain-containing protein

Macromolecule #17: Utp30

• Macromolecule: Name: Utp30 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 43.700527 KDa

Sequence

String:

MAPSTAVAKK ETDAVIPVDP DQTLKACKAL LAHIKKAAAA PRPDGKQNLL ADEESTVAET PIWLTLTTKK HIHDSHRLQP GKIILPHPL NTSEEISVCL ITADPQRFYK NAVADEFPED LRAKIGRVID ISHLKAKFKA YEAQRKLFSE HDVFLADTRI I NRLPKALG KTFYKTTTKR PIPVVLMAQR EKVNGKRVPA PKGKKEKRDP LENANARPIP EIVAEIRKAI GAALVHLSPS TN TAIKVGY ANWEPEKLAA NIETVIRELV ERFVPQKWQN VRNFYVKGPE TAALPIYQTD ELWLDESKVV PDGQEPARAL PGK REKANI GKKRKPLEDA SQPALEETGK DERPKKKAKK TLPESNDDKL DKAIAERKEQ LKKQKAAAKK VAADI

UniProtKB: Uncharacterized protein

Macromolecule #18: Nop1

• Macromolecule: Name: Nop1 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 32.960844 KDa

Sequence

String:

MGFERGGRGG GRGGAAARGG RGGARGGRGG PAGRGGPAGR GRGGPAGRGR GGRSGRGGKP KAKGAKAGKK VIVEPHRHKG VFVARGGKE DLLCTANLVP GESVYGEKRI SVETPGSGPD AVATKTEYRI WNPFRSKLAA GILGGLETIY MKPGSKVLYL G AASGTSVS HVADIVGPTG AVYAVEFSHR SGRDLINMAT RRTNVIPIVE DARKPMAYRM LVPMVDVIFA DVAQPDQARI VG INARLFL KQGGGLLISI KASCIDSTAP PEQVFASEVQ KLREDKFFPK EQLTLEPYER DHAMVSCVYL QKEFEG

UniProtKB: rRNA 2'-O-methyltransferase fibrillarin

Macromolecule #19: Putative nucleolar protein

• Macromolecule: Name: Putative nucleolar protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 57.830699 KDa

Sequence

String:

MVVVNFLLFE SAVGFSLFEV VHQADTVGLE LPEVKDAMKT LDKFGKMVKL RSFNPWTSAA QGLEAINLIS EGIMPEYLKS ALEMNLPQT SGKKSKVVLG VADKKLAGEI TAAFPGVQCE AADTSEVVAA LLRGIRTHAN KLHKSLQEGD IGRAQLGLGH A YSRAKVKF SVHKNDNHII QGIATLDALD KSINQGAMRV REWYGWHFPE LIRIVSDNIT YAKVVLAIGN KSSLTDESVD DL ANVLNQD QDKALAIIQA AKVSMGQDIS EVDLQMVRDL ASNVTSMADY RRILAESLDK KMSEVAPNLQ VILGTPVAAR LIA HAGSLT NLAKYPASTL QILGAEKALF RALKTKSATP KYGLLYQSSF IGRAGPKVKG RISRYLANKC SIASRIDNFS EKPT RHFGE VLRQQLEQRL EWYAKGTKPM KNSEAMEKAI KAVMADDEET LPVAVDAMDI DSKSPAKEKK DKKEKKEKKE KKEKK KEDK EEKKKDKKRK SLGGEDVEMA DADVGENKKK KKRKSEIAE

UniProtKB: Nucleolar protein 56

Macromolecule #20: Nop58

• Macromolecule: Name: Nop58 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 64.292113 KDa

Sequence

String:

MPLFILTETS AGYALFKAAD KKLLDSDNVS ERLSTLDKII KEIKYKEFAK FDSAAIAVEE ASGILEGKVT PKLASLLNEL KDEKKVTLA VHDTKLSNSI TKLPGINIKP ISGSMTDDLF RAIRQHLYNL IPGMEPSNFD EMNLGLAHSL SRHKLKFSPE K VDVMIVHA VALLDELDKE LNVMAMRVKE WYGWHFPELG KILPDNLSYA RVVLALGLRT NAPNADLSEI LPPEIEAAVK AA ADISMGT EISTEDYENI KLLAVQVVER SEYRRQLAEY LQNRMKAISP NMTELIGALV GARLIAHSGS LVNLAKNPGS TIQ ILGAEK ALFRALKTKH ATPKYGIIYH ASLVGQASGP NKGKIARQLA AKIALSVRTD AFEDFPENAD DETRAAVGIQ ARAK LENNL RLLEGKPLNK GVALGPNGIP VGMPAKWDVK EARKYNIEAD GLATTTSKES SEQPKRPLIE EVPEVEMKDA SASDK KDKK EKKKEKKEVA SSKITEADYE RIAKELGMSL SKFTKKLEKG KIKIKPDGSV EVKGKDGEDA EAETPVKSKS SKRKHE PEE ETPAKEEKHK KKKKKSSKE

UniProtKB: Nucleolar protein 58

Macromolecule #21: Snu13

• Macromolecule: Name: Snu13 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 13.831854 KDa

Sequence

String:

MSNNESAAWP KAEDPALVQE LLDCVQQASH YRQLKKGANE TTKSVNRGTS ELVILAADTQ PLSIVLHIPL ICEEKNVPYV YVPSKVALG RACGVSRAVI AVSLTSNEAS DLNSKIRALR DKVERLAM

UniProtKB: H/ACA ribonucleoprotein complex subunit 2

Macromolecule #22: Rrp9

• Macromolecule: Name: Rrp9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 69.610438 KDa

Sequence

String:

MSSFFTAPAS EKKRKRAATA DAPKKRLATT KSSSKSGSKA PTKAAAPASK KKAIERDESI SGSDLDSDLS GDDEFIERRS SDAGSGDES EKEGETAAEK RLRLAQRYLE KTRKEVEQLD EYAFDAEEID RDLLAERLKE DVAEAKGKVY RRLASELAFD K ATYTQFRW NSGTVTSVAV CPPYAYTTTK DGYLTKWKLQ DLPKNQWPQT TKKKPKKPPA PPKKRPERIC FAKADARKAN DK TYQGHLK APLVVKASQD GKFVVTGGAD KRLVVYNAAD LKPIKAFTQH RDAVTGLAFR RGTNQLYSCS KDRTVKVWSL DEL AYVETL FGHQDEILDI DALGQERCVS VGARDRTARY WKVPEESQLV FRGGGEGGSS NTKKHKLPPG MDPASAAHEG SMDR VAMID DDMFVTGSDN GDLALWSIQR KKPLHVIARA HGLEPPIKLE DYSADEIPDP SIIPAPQPRG ITALRTLPYS DLIFS GSWD GCIRVWRLSE DKRKLEAVGI LGVGSETCEN STNISNGATS NGESSSTSSS TTLAAQSSSS SSPPPSQPKD LVRGIV NDI ALFERGERGR DGLCVVVVTG KEMRFGRWKY MKEGRCGAVI FEVPKVEKKN KRKNEDKKEE VNGVYKE

UniProtKB: Ribosomal RNA-processing protein

Macromolecule #23: RNA 3'-terminal phosphate cyclase-like protein

• Macromolecule: Name: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 43.399766 KDa

Sequence

String:

MAEPKPEFLR FTGHRAFTQR LVLATLYGRP IHISKIRSSS ATNPGLAPHE ISFLRLLESV TNGSIIDVSY SGTTITYQPG LITGTVPGM NASLSSDAIE HVIPATNTRG ITYFLIPLAL LAPFSKAHLN VRFTGPGVIT SATHGARDLS IDTFRTAVLP L YGLFGIPP ARIELRVLQR SCAGPGGKGG GGIVEMRFAS QVRLPKTLHL NRRPGKVRRI RGVAYCTGVA ASHNNRMITA AR GVLNQLV SDVHIAAQYD PAPLVAEKGT TQKKKTGIGF GLSLVAETSA EGVIYAADEV APPEGGVVPE DIGEKCAYQL LDV IAQGGC VMAASAPTVL TLMAMGSEDV GRLRLGRRVV SPELLELARD LKAFGAASWG IRDAGDDEDD AEGELGDLIV SVKG TGVGN VGRKVA

UniProtKB: RNA 3'-terminal phosphate cyclase-like protein

Macromolecule #24: Bms1

• Macromolecule: Name: Bms1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 134.17225 KDa

Sequence

String:

MDSQQHKPHR PSKTKEKKKK QNSGGTNPKA FAVANPGKLA RQAARSHDIK EKRLHVPLVD RLPDEPPPRL VVIVGPPGVG KTTLLKSLV RRYTKETMSD PVGPITVVTS KKQRLTFIEC PNELEAMIDM AKVADIVLLM IDGNYGFEME TMEFLNILAN T GMPGNVFG ILTHLDLFKK PSALKDAKKR LKHRLWTELY QGAHLFYLSG VLNGRYPDRE IHNLSRFLSV MKNPRPLVWR NT HPYTIID NYRDITHPTK IEEDPLCDRT IELSGYLRGT NFAAQGQRVH IAGVGDFTIS KIEELPDPCP TPAMEKAMAN LTG KKPRRR LDEKDKKLWA PMADRSGMKI SGDHIVITRE KGFTFDKDAN VERGEGEQLI VDLQGEKKLL GQTDKGVKLF AGGE QLTQI PEEDTNDTGR KTHRKARFLE DDRQNDENGV PEDEGFVSGE ESKGSDGSDI EEEFDEKRLG KMFRGDDEET QDEDV VFAD TDSELGSISG DEGVDSEESG SDEEFDSEEE EAVRWKENMM ERARALHGKR KPWRAIDLAR LMYDTTLTPA QALRRW RGE DNEKEEEEEE DIEKDEDTFF HKAKDEDDLE EDRMIPHFDY EELKTKWSNP ENIDALRRTR FSTGRPKGDG EGDSEGE GD GDDDDDFNGF DEDDEDEGDG AFEDLETGEK HGPSKEKKEE EEKPAMSLEE ERERNARRKE ELRARFEEED REGLLNDK A IARREGGLDE EFGEDQWYEM QKAMLQKQLD INKAEYAELD EHQRRQVEGY RAGKYARLVI EGVPAEFCKN FQPRMPILV GGLSATEDRF GFVQVRIKRH RWHKKILKTG DPLIFSLGWR RFQTLPIYSI WDNRTRNRML KYTPEHMHCF GTFWGPLIAP NTSFCCFQS FSASNPGFRI AATGTVLSVD ESTEIVKKLK LVGTPWKIFK NTAFIKDMFN SSLEIAKFEG AAIRTVSGIR G QIKRALSK PEGYFRATFE DKILLSDIVI LKAWYPVKPK QFYNPATNLI GWQSMRLTGE IRRAENIPTP QNPNSTYRKI ER PERHFNP LRVPKNLAAE LPFKSQIVQT KPQKKETYMQ KRAVVVGREE RKLRDLMQKL TTIRKEKIAK RKAKKEAQRE KLK KELAEI EERRREKQKK EKKEFWEREG KKRKASEEWG GGGKRRK

UniProtKB: Bms1-type G domain-containing protein

Macromolecule #25: Imp3

• Macromolecule: Name: Imp3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 21.802396 KDa

Sequence

String:

MVRKLKYHEQ KLLKKHDFIN YKSDNNHRDH DVIRRYMIQK PEDYHKYNRL CGSLRQFAHR LSLLPPDNEV RRKHETLLLD KLYDMGILS TKAKLSAVEH NVTVSAFARR RLPVVMTRLR MAETVQAATK LIEQGHVRVG VEEVRDPAFL VTRNMEDFVT W TVGSKIKQ NIMKYRDKLD DFELL

UniProtKB: Uncharacterized protein

Macromolecule #26: Putative U3 small nucleolar ribonucleoprotein

• Macromolecule: Name: Putative U3 small nucleolar ribonucleoprotein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 34.216266 KDa

Sequence

String:

MLRKQARQRR DYLYRRALLL RDAEIAEKRA KLRAALASGK PLDPKIANDK ELRKDFDYDV SRDIAKEQGE IDIDDEYSEL SGIVDPRVL VTTSRDPSSR LMAFSKEIRL MFPTAIRLNR GNLILPDLVM SAQRERLSDI ILLHEHRGTP TAITISHFPH G PTLMASLH NVVLRADIPK SIKGTVSESY PHLIFEGFRT PLGQRVVKIL KHLFPPRDPT NNAKSGNRVI TFVNQDDCIE VR HHVYVRT NYNSVELSEV GPRFTMRPFS ITMGTLENKD ADVEWHLSQY TRTGRKKNYF

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP4

Macromolecule #27: Putative U3 small nucleolar ribonucleoprotein protein

• Macromolecule: Name: Putative U3 small nucleolar ribonucleoprotein protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 86.082141 KDa

Sequence

String:

MPGAPSTTSS FTSTSHTLSA LPSMPQSLSA SAADGSGSGS DGAAAAAITA FLDSTAPENR HIFLRPTPQL PAGSLALLKA ALDPLAAQI ADHQAAGIAR LRESGALSSK KRKRDGSEKE NKPAALKIRK VHVDGFETQQ VWQQARKIIT SALGEAQAVL E ELKVNGEV EEEEGEDKVI EFGEDGFEVG SSDEEESEEE GNEEADTEDS DGEGASLGDE NAMFDLEAEE DSGSEEDKSD VG EEVDGEV DGEKHSDLDG EEEGEEGEED EEDEEDDDES AEDLVEDPHG LNDGFFSIDE FNKQTQMWED QDMRAEPTAE LDD DSEDID WHADPFAVKP SKRGKKDDGD MDLDDEEDES DDEAPPVGKK ALEKMLDKDE DDEGGNLEDD LADGMGMDLT ANDI YYKDF FAPPRKKKKP GSSKKKRELE LETKRPDDAD VERAEQDVRR DLFDDLSEHE DSEDALSDAS AGDPKSRKSA HERRQ AKIA EQIRKLEAEL VAKRAWTLAG EATAADRPVN SLLGEDMEFD HVGKPVPVVT EEVSESIEEL IKRRILAGEF DEVLRR RPD MFGNPHGVRR GLVDVEDTKA KQSLAEIYEE EAVKKANPDA YVSAADEKLR RDEEEIKRMW KEISAKLDAL SSWHYKP KP PAPTLTVVSD VATVAMEDAQ PATAQGVAGG ETSMIAPQEV YAPSKDTAEK GEVVTKAGIP IAKQEMSREE KLRRRRRE K ERIRKAGGLD GGKPVSEKEK EKKETVAQLK KSGVKVINRK GEVVGLDGKK VGEKKVQSSG AYKL

UniProtKB: Putative U3 small nucleolar ribonucleoprotein protein

Macromolecule #28: Sof1

• Macromolecule: Name: Sof1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 50.869828 KDa

Sequence

String:

MKIKALTRSI TAQQAPGSDV QRAPRNLAPE LHPFERAREY QRALNAVKLE RMFAKPFLGQ LGNGHVQGVY SMCKDKNSLN CIASGSGDG VVKVWDLTTR DEETWRVAAH NNIVKGLTFT NDKKLLSCAT DGIKLWDPYA SPSNTTPIAT WQEGGPYTSL S FHRSANTF AASSGQGCIR IWDLEHSTAG QAIQWPSFVD TITDVCFNQV ETSVIGSVAT DRSIILFDLR TNMPVIKTVL HF ACNRIVF NPMEAMNLAV ASEDHNIYIF DARNFDKALN IQKGHVAAVM DVEFSPTGEE LVSGSYDRTI RLWRRDAGHS RDV YHTKRM QRVFRTMWTM DSKYILTGSD DGNVRLWRAN ASERSGVKAT RQRQALEYNN ALLDRYGHLP EIRRIRRHRH LPKV VKKAT EIKREELAAI KRREENERKH SNKKYEKRKS EREKAVLVKQ Q

UniProtKB: DDB1- and CUL4-associated factor 13

Macromolecule #29: Emg1

• Macromolecule: Name: Emg1 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 27.936164 KDa

Sequence

String:

MQSQTAGTQS LPPPALPQLV AEQHVPIPPN DKDTKRLIVV LSNASLETYK ASHGTNRNGV REEKYTLLNS DEHIGIMRKM NRDISDARP DITHQCLLTL LDSPINKAGK LQIYIQTAKG VLIEVSPTVR IPRTFKRFAG LMVQLLHRLS IKGTNTNEKL L KVIQNPIT DHLPPNCRKV TLSFDAPLVR VRDYVDTLGP NESICVFVGA MAKGPDNFAD AYVDEKISIS NYSLSASVAC SK FCHACED AWDII

UniProtKB: Nucleolar essential protein 1

Macromolecule #30: KRR1 small subunit processome component

• Macromolecule: Name: KRR1 small subunit processome component / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 37.500777 KDa

Sequence

String:

MPSTHKKDKP WDTDDIDKWK IEPFLPEHSS GPFLEESSFM TLFPKYRERY LKDCWPLVTK ALEKHGIAAT LDIVEGSMTV KTTRKTYDP AAILKARDLI KLLARSVPAP QALKILEDGM ACDIIKIRSM VRNKERFVKR RQRLLGQNGT TLKALELLTQ T YILVHGNT VSVMGGYKGL KEVRRVVEDT MNNIHPIYLI KELMIKRELA KDPALAHEDW SRYLPQFKKR TLSKRRKPFK IN DKSKKPY TPFPPAPEKS KIDLQIESGE YFLSKEAKQR AAEAERAEKA RQKKEEKKRE REKEFVPPEE DGGKKKKRKV KHG EE

UniProtKB: KRR1 small subunit processome component

Macromolecule #31: Pre-rRNA-processing protein PNO1

• Macromolecule: Name: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 28.715047 KDa

Sequence

String:

MPAPTALKQP PPAPEQQAAP AITNENEDEL LIDIQQAAAT LTDPNAAEPP EETMENEMAV DEEGRPRFAP GKNIDPIRRI ETRKIPIPP HRMSALKANW TKYPPLVDHC KLQVRMNIKE KRVELRSSKY TVSNEALQMG ADFVSAFAMG FDIDDAIALL R LDSLYIQS FDIKDVRQTL GPDALSRAIG RIAGKDGKTK FAIENATKTR IVLAGSKVHI LGAFENIGMA RESIVSLVLG AQ PGKVYNN LRIIASRMKE RW

UniProtKB: Pre-rRNA-processing protein PNO1

Macromolecule #32: RNA cytidine acetyltransferase

• Macromolecule: Name: RNA cytidine acetyltransferase / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 119.795125 KDa

Sequence

String:

MTVQKTVDSR IPTLIRNGLQ TKKRSFFVVV GDHAKEAIVH LYYIMSSMDV RQNKSVLWAY KKELLGFTSH RKKREAKIKK EIKRGIREP NQADPFELFI SLNDIRYCYY KETDKILGNT YGMCILQDFE AITPNILART IETVEGGGLV VLLLKGMTSL K QLYTMTMD VHARYRTEAH DDVIARFNER FLLSLGSCES CLVIDDELNV LPISGGKGVK PLPPPDEDEE LSPAAKELKK IK DELEDTQ PIGSLIKLAR TVDQAKALLT FVDAIAEKTL RNTVTLTAAR GRGKSAAMGV AIAAAVAYGY SNIFITSPSP ENL KTLFEF VFKGFDALDY KDHADYTIIQ STNPEFNKAI VRVNIHRNHR QTIQYIRPQD AHVLGQAELV VIDEAAAIPL PLVK KLMGP YLVFMASTIS GYEGTGRSLS LKLIKQLREQ SRAGANPNGG NAVEVDRSTL KATKETTSVG GRSLKEITLS EPIRY AQGD NVEKWLNTLL CLDATLPRSK ISTTGCPDPS QCELLHVNRD TLFSFHPVSE KFLQQMVALY VASHYKNSPN DLQLMS DAP AHELFVLTGP IQEGRLPEPL CVIQVSLEGK ISKQSILKSL SRGQQPAGDL IPWLVSQQFQ DDEFASLSGA RIVRIAT NP DYMSMGYGSK ALQLLVDYYE GKFADLSEDA AAEVPRSIPR VTDAELSKGS LFDDIKVRDM HELPPLFSKL SERRPEKL D YVGVSYGLTQ QLHKFWKRAQ FVPVYLRQTA NDLTGEHTCV MIRPLQDGND PSWLGAFAAD FHKRFLSLLS YKFREFPSI LALTIEESAN AGAMLDPSNA PTELTKAELD QLFTPFDHKR LESYANGLLD YHVVLDLMPT IAQLYFTGRL REAVKLSGLQ QAILLALGL QRKDIDTLAT ELNLPGSQVL AIFMKIMRKV TQHFGALVSG AIAAELPDPN KTVGVSKENA MGIHDDEVVG L KFEALEQR LEDELDEGGD EALRELRKKQ RELIDSLPLD QYEIDGDDDA WKEAEKRVAS AAKSGKKVDG TLVSVPSAKA AK RKAEEMA ALRDELEKME KGKERGSKKA KKEKRR

UniProtKB: RNA cytidine acetyltransferase

Macromolecule #33: Fcf2

• Macromolecule: Name: Fcf2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 22.569129 KDa

Sequence

String:

MATTLGLPDE EIDRLLAEAE ARLAGSGDAD AGAIALAKPA ASKPLTVAAP AAPKAGEQTV PQVKKAEELS VRVPQLPQKK KGPPDTLSD WYNIPRTNLT PELKRDLQLL RMRDVVAMGK QFFKKDNRKD FVPEYCQVGT IIAGATDGVS GRLTRKERKR T IVEEVLSS DSVSKYKRKY HEIQEHKKSG RKGYYKKLMA ARKRK

UniProtKB: Fcf2 pre-rRNA processing C-terminal domain-containing protein

Macromolecule #34: 40S ribosomal protein S1

• Macromolecule: Name: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 29.245158 KDa

Sequence

String:

MAVGKNKRLS KGKKGLKKKV QDPFTRKDWY NIKAPAPFAV RDVGKTLVNR TTGLKNANDA LKGRIFEVCL ADLQKDEDHA FRKIKLRVD EVQGKNCLTN FHGLDFTTDK LRSLVRKWQT LIEANVTVTT TDHYLLRLFA IAFTKRRPNQ IKKTTYAQSS Q IRAIRRKM VEIIQREAAS CTLHQLVSKL IPEVIGREIE KATQGIYPLQ NVHIRKVKLL KQPKFDLGAL MALHGESSEE AG QKVEREF REQVLESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #35: 40S ribosomal protein S4

• Macromolecule: Name: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 29.800764 KDa

Sequence

String:

MARGPKKHQK RLSAPSHWLL DKLSGAYAPR PSTGPHKLRD CMPLIVFVRN RLKYALNYRE TKAIMMQRLV KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRITDEEA KYKLGKVKRV QLGRGGVPFL VTHDARTIRY PDPLIKVNDT V KIDLETGK ITDFIKFDTG ALAMITGGRN MGRVGVITHR ERHDGGFGIV HLKDALDNTF ATRESNVFVI GSEKPWISLP KG KGVKLTI AEERDQRRAR ALAAAGH

UniProtKB: 40S ribosomal protein S4

Macromolecule #36: 40S ribosomal protein s5-like protein

• Macromolecule: Name: 40S ribosomal protein s5-like protein / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 23.679225 KDa

Sequence

String:

MSEGEVEVAQ PQYDVLPKEV LAEVGSVKLF NRWSYDDIEI RDISLTDYIQ IRAPVYIPHS AGRYAVKRFR KANCPIIERL TNSLMMHGR NNGKKLMAVR IVAHAFEIIH LMTDQNPIQV AVDAIVNCGP REDSTRIGSA GTVRRQAVDV SPLRRVNQAI A LLTTGARE AAFRNVKTIA ECLAEELINA AKGSSNSYAI KKKDELERVA KSNR

UniProtKB: 40S ribosomal protein s5-like protein

Macromolecule #37: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 27.490139 KDa

Sequence

String:

MKLNISHPAN GSQKLIEVED ERKLRHFYDK RMGAEVAGDP LGPEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSEG HSCYRPRRD GERKRKSVRG CIVGPDLSVL ALSIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFSLSK DDDVRKYVIR R EVQPKGEG KKPYTKAPRI QRLVTPQRLQ HKRHRIALKR RQQEKVKEEA AEYAQILAKR VAEAKAQKAD LRKRRASSLH K

UniProtKB: 40S ribosomal protein S6

Macromolecule #38: 40S ribosomal protein S7-like protein

• Macromolecule: Name: 40S ribosomal protein S7-like protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 23.08465 KDa

Sequence

String:

MSAPSLNKIA ANSPSRQNPS DLERAIAGAL YDLETNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PALQGFHRVQ QRLTRELEK KFSDRHVLIL AARRILPKPK RSARSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRTKEDGSKV L KVILDEKE RGGVDYRLDT YSEVYRRLTG RNVTFEFPQT TITDY

UniProtKB: 40S ribosomal protein S7

Macromolecule #39: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 23.102416 KDa

Sequence

String:

MGISRDSRHK RSHTGAKRAF YRKKRAFELG RQPANTRIGP KRIHIVRTRG GNHKYRALRL DSGNFAWGSE GCTRKTRIIG VVYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAAR FAAHGKVDPA L EKQFEAGR LYAIISSRPG QSGRADGYIL EGEELAFYQR KLHK

UniProtKB: 40S ribosomal protein S8

Macromolecule #40: 40S ribosomal protein s9-like protein

• Macromolecule: Name: 40S ribosomal protein s9-like protein / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 22.029836 KDa

Sequence

String:

MAPRKYSKTY KVPRRPYEAA RLDSELKLVG EYGLRNKREV WRVLLTLSKI RRAARILLTL DEKDPKRLFE GNALIRRLVR IGVLDESRM KLDYVLALKA EDFLERRLQT LVYKLGLAKS IHHARVLIRQ RHIRVGKQIV NVPSFMVRLD SQKHIDFALT S PFGGGRPG RVRRKKAKAA EGGEGGEEEE EE

UniProtKB: 40S ribosomal protein s9-like protein

Macromolecule #41: 40S ribosomal protein S13-like protein

• Macromolecule: Name: 40S ribosomal protein S13-like protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 16.912891 KDa

Sequence

String:

MGRLHSKGKG ISASAIPYSR NPPAWLKTTP EQVVEQICKL ARKGATPSQI GVILRDSHGI AQVKVVTGNK ILRILKSNGL APDIPEDLY FLIKKAVAVR KHLERNRKDK DSKFRLILIE SRIHRLARYY KTVGVLPPTW KYESSTASTL VA

UniProtKB: 40S ribosomal protein S13-like protein

Macromolecule #42: 40S ribosomal protein S14-like protein

• Macromolecule: Name: 40S ribosomal protein S14-like protein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 16.071464 KDa

Sequence

String:

MPPKKTTRPA QENISLGPQV REGELVFGVA RIFASFNDTF VHVTDLSGRE TICRVTGGMK VKADRDESSP YAAMLAAQDV AARCKELGI TALHIKIRAT GGNGTKTPGP GAQSALRALA RSGMKIGRIE DVTPTPSDST RRKGGRRGRR L

UniProtKB: 40S ribosomal protein S14-like protein

Macromolecule #43: 40S ribosomal protein S16-like protein

• Macromolecule: Name: 40S ribosomal protein S16-like protein / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 15.962771 KDa

Sequence

String:

MASVQAVQVF GKKKNATAVA RCVQGKGLIK VNGKPLKLFA PEILRAKLYE PILILGTDKF ADVDIRIRVA GGGHTSQVYA VRQAIAKSI VAYYAKYVDE HSKNLLKQEL IQFDRSLLVA DPRRCEPKKF GGRGARARFQ KSYR

UniProtKB: 40S ribosomal protein S16-like protein

Macromolecule #44: 40S ribosomal protein S11-like protein

• Macromolecule: Name: 40S ribosomal protein S11-like protein / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 18.698182 KDa

Sequence

String:

MATELTVQSE RAFLKQPHIF LNSKVKVKST RPGKGGRRWY KDVGLGFKTP KTAIEGHYID KKCPFTGMVS IRGRILTGRV VSTKMHRTI IIRREYLHYI PKYNRYEKRH KNLAAHVSPA FRVEEGDMVV VGQCRPLSKT VRFNVLRVLP RTGKSVKKFQ K F

UniProtKB: 40S ribosomal protein S11-like protein

Macromolecule #45: 40S ribosomal protein S22-like protein

• Macromolecule: Name: 40S ribosomal protein S22-like protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 14.905471 KDa

Sequence

String:

MVRTSVLHDA LNSINNAEKM GKRQVMIRPS SKVIVKFLQV MQRHGYIGEF EEVDNHRSGK IVVQLNGRLN KCGVISPRYN VRLAELEKW VTKLLPARQF GYVILTTSAG IMDHEEARRK HVAGKIIGFF Y

UniProtKB: 40S ribosomal protein S22-like protein

Macromolecule #46: 40S ribosomal protein s23-like protein

• Macromolecule: Name: 40S ribosomal protein s23-like protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 15.934653 KDa

Sequence

String:

MSGGKPRGLN AARKLRNNRR EQRWADLQYK KRALGTAYKS SPFGGSSHAK GIVLEKVGVE AKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VGLLALWKEK KEKPRS

UniProtKB: 40S ribosomal protein s23-like protein

Macromolecule #47: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 15.535286 KDa

Sequence

String:

MADTDSPVTL RTRKFIRNPL LSRKQMVVDI LHPGRPNISK DELREKLATM YKAQKDQVSV FGLRTQYGGG KTTGFALIYD SPEALKKFE PRYRLVRAGL AAKVEKASRQ QRKQRKNRLK TLRGTAKVKG AKAKKEK

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #48: 40S ribosomal protein S28-like protein

• Macromolecule: Name: 40S ribosomal protein S28-like protein / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 7.74198 KDa

Sequence

String:

MDSSKAPVKF VKVTRVLGRT GSRGGVTQVR VEFMDDTTRS IIRNVKGPVR ENDILVLLES EREARRLR

UniProtKB: 40S ribosomal protein S28-like protein

Macromolecule #49: Faf1

• Macromolecule: Name: Faf1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 34.576586 KDa

Sequence

String:

MPGLLGKRKS RAEEDPEAVA KAQELLRKHF EAQFKPIDLA PLPRRAIESE DEEDESSEEG SDVNSGEGDE WDGISGDEDG TESEGDESD DEPHVVQVVD YSNDSSAADG KMSKQELKVY LSSRPPDPTR KSSSSKPKPS KKSTDDSFPE DSAELLANDL A LQRLIAES HILSEAGANP SHWQSSHAAT TGTNTRAFAT GRIAKKTTDM RIQALGAKES ILTQQKMPMN MRKGIVKHQE EK EKKRRQE ARENGIVLER EVKKKKTVRK RRERPVDLPA VGRMRGAELR ISAKEAAAIA REVRGPQGRG KRRR

UniProtKB: Uncharacterized protein

Macromolecule #50: 35S rRNA

• Macromolecule: Name: 35S rRNA / type: rna / ID: 50 / Number of copies: 1
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 827.801812 KDa

Sequence

String:

GGGGGGAUGU CCGGGUCGCC CUGGGCACCU CUCCGCGGCA GCCUUCGCGG GCAAGCCGUA GUCCCGGUUC AUACGUCGCC GCAAUCGCG AGGGGACCAA CGGGUAACCG UUCCUCCAAG CGGUCGCCGG CGAGCACCAC CCCAGCCCCG CCGCCGCCCC C AGCGCACC GCGCGCAGGG GGGCCGGCGC GGCAUCUGGG AAGACUCCUA AGGCGCAAGG GAAUACCCCG UGUGCGCCGG GG GUUGGGC UCUGUGGGUG CUUGGCCGCU GGCCAGACCU ACUUCAAAGC CACCGGGGGU ACCUACCGGU UGGGGAAGCC CUA GCGGCU GAACCUCUCG GUCGGGCCUC CUCUUGGGGC GCCUAGGCGG UGUCUUCGGG UCGCGUGCAG CCUCCCGUCC UCGC CUGCC GACAGCGCUC UGGGGAAUUC AGGAGGGGAA AGCAGAUGUG CCCCGCGGCG AGACUGGCGC ACCGCCGGAC GAGCU GCUG GGGCACGCGG GUACGCUAGC CGCUAGCGCC CGCCAGCCUG UAACUAGAGU GAACUCGGCC GCCCUGAAAC ACGGGC GGU UGGCCUGCUU AGGCGCACGA UAGUUACCUG GUUGAUUCUG CCAGUAGUCA UAUGCUUGUC UCAAAGAUUA AGCCAUG CA UGUCUAAGUA UAAGCAAUUA UACAGCGAAA CUGCGAAUGG CUCAUUAAAU CAGUUAUCGU UUAUUUGAUA GUACCUUA C UACAUGGAUA ACCGUGGUAA UUCUAGAGCU AAUACAUGCU GAAAAUCCCG ACUUCGGAAG GGAUGUAUUU AUUAGAUUA AAAACCAAUG CCCUUCGGGG CUUUCUGGUG AUUCAUAAUA ACUUCUCGAA UCGCACGGCC UUGCGCCGGC GAUGGUUCAU UCAAAUUUC UGCCCUAUCA ACUUUCGACG GCUGGGUAUU GGCCAGCCGU GGUGACAACG GGUAACGGAG GGUUAGGGCU C GACCCCGG AGAAGGAGCC UGAGAAACGG CUACUACAUC CAAGGAAGGC AGCAGGCGCG CAAAUUACCC AAUCCCGACA CG GGGAGGU AGUGACAAUA AAUACUGAUA CAGGGCUCUU UCGGGUCUUG UAAUUGGAAU GAGUACAAUU UAAAUCCCUU AAC GAGGAA CAAUUGGAGG GCAAGUCUGG UGCCAGCAGC CGCGGUAAUU CCAGCUCCAA UAGCGUAUAU UAAAGUUGUU GAGG UUAAA AAGCUCGUAG UUGAACCUUG GGCCUAGCCC GCCGGUCCGC CUCACCGCGU GCACUGGCUG GGCUGGGCCU UUCCU UCUG GAGAACCGCA UGCCCUUCAC UGGGUGUGUC GGGGAACCAG GACUUUUACU CUGAACAAAU UAGAUCGCUU AAAGAA GGC CUAUGCUUGA AUACAUUAGC AUGGAAUAAU AGAAUAGGAC GUGUGGUUCU AUUUUGUUGG UUUCUAGGAC CGCCGUA AU GAUUAAUAGG GACAGUCGGG GGCGUCAGUA UUCAAUUGUC AGAGGUGAAA UUCUUGGAUU UAUUGAAGAC UAACAACU G CGAAAGCAUU CGCCAAGGAU GUUUUCAUUA AUCAGGAACG AAAGUUAGGG GAUCGAAGAC GAUCAGAUAC CGUCGUAGU CUUAACCAUA AACUAUGCCG AUUAGGGAUC GGACGGCGUU UAUUUUUGAC CCGUUCGGCA CCUUACGAUA AAUCAAAAUG UUUGGGCUC CUGGGGGAGU AUGGUCGCAA GGCUGAAACU UAAAGAAAUU GACGGAAGGG CACCACCAGG GGUGGAGCCU G CGGCUUAA UUUGACUCAA CACGGGGAAA CUCACCAGGU CCAGACACGA UGAGGAUUGA CAGAUUGAGA GCUCUUUCUU GA UUUCGUG GGUGGUGGUG CAUGGCCGUU CUUAGUUGGU GGAGUGAUUU GUCUGCUUAA UUGCGAUAAC GAACGAGACC UUA ACCUGC UAAAUAGCCC GUAUUGCCUU GGCAGUACGC CGGCUUCUUA GAGGGACUAU CGGCUCAAGC CGAUGGAAGU UUGA GGCAA UAACAGGUCU GUGAUGCCCU UAGAUGUUCU GGGCCGCACG CGCGCUACAC UGACAGAGCC AGCGAGUACU CCCUU GGCC GAGAGGCCCG GGUAAUCUUG UUAAACUCUG UCGUGCUGGG GAUAGAGCAU UGCAAUUAUU GCUCUUCAAC GAGGAA UCC CUAGUAAGCG CAAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUACU ACCGAUU GA ACGGCUCAGU GAGGCUUUCG GACUGGCCCA GAGAGGUCGG CAACGACCAC UCAGGGCCGG AAAGUUAUCC AAACUCGG U CGUUUAGAGG AAGUAAAAGU CGUAACAAGG UCUCCGUAGG UGAACCUGCG GAGGGAUCAU UAAUGAGUUA CCACAACUC CCAAACCCAU UGUGAACCUA CCCUUCACGU UGCUUCGGCG GGUCGGGCGC UGUGCCGCCC CUCCGGGCGA CCCGGGGCUC CCCAAAGGC CCCGGGCCCG CCGCCCGCCG GAGGUACGCA AACUCUUGUU CAUUGUACGG CCUCUCUGAG UGAAGUACUG A AUAAGUCA

Macromolecule #51: U3 snoRNA

• Macromolecule: Name: U3 snoRNA / type: rna / ID: 51 / Number of copies: 1
• Source (natural): Organism: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
• Molecular weight: Theoretical: 87.992648 KDa

Sequence

String:

AGCGACAAUA CUUCAGAGAA UCAUUUCUAU AGUAGUUGUC CUCUCUUGUG UUUCCUAAAG GAGCCACAGA UCCCACCCGG GUUGAUGAA CGAGAUCCUC GGCGCCCAAC AGUGAGGUCC AUUAUUUACU CUCGCUCUAC CUGCAAAGGU GGCGGUCGCG U GCCUCGUC UCGCGGCUGU AUAGAGAGUG GCGAUGAUCU GUACCCCGGC UUUCCUGAGG UCUUGCCUUC GGGCGCUCAG GC GGGGUGG GUGUCGAUGG AAGUCUGACC GGCCAUU

Macromolecule #52: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 52 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.4 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: EMDB MAP
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 231121
• Initial angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)
• Final angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)

Atomic model buiding 1

• Refinement: Protocol: AB INITIO MODEL

Output model

PDB-5oql:
Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum