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- PDB-5okz: Crystal Strucrure of the Mpp6 Exosome complex -

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Basic information

Entry
Database: PDB / ID: 5okz
TitleCrystal Strucrure of the Mpp6 Exosome complex
Components
  • (Exosome complex component ...) x 9
  • M-phase phosphoprotein 6 homolog
KeywordsRNA BINDING PROTEIN / RNA degradation Exosome Ribosome biogenesis KH-domain
Function / homology
Function and homology information


nuclear mRNA surveillance of spliceosomal pre-mRNA splicing / nuclear mRNA surveillance of mRNA 3'-end processing / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / mRNA decay by 3' to 5' exoribonuclease / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent CUT catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / U5 snRNA 3'-end processing / cytoplasmic exosome (RNase complex) ...nuclear mRNA surveillance of spliceosomal pre-mRNA splicing / nuclear mRNA surveillance of mRNA 3'-end processing / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / mRNA decay by 3' to 5' exoribonuclease / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent CUT catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / U5 snRNA 3'-end processing / cytoplasmic exosome (RNase complex) / TRAMP-dependent tRNA surveillance pathway / CUT catabolic process / U4 snRNA 3'-end processing / nuclear polyadenylation-dependent rRNA catabolic process / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / : / poly(A)-dependent snoRNA 3'-end processing / exosome (RNase complex) / nuclear exosome (RNase complex) / nuclear-transcribed mRNA catabolic process, non-stop decay / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / : / rRNA catabolic process / nuclear mRNA surveillance / RNA catabolic process / poly(U) RNA binding / rRNA metabolic process / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA catabolic process / RNA processing / mRNA processing / manganese ion binding / protein-containing complex binding / nucleolus / RNA binding / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
M-phase phosphoprotein 6 / M-phase phosphoprotein 6 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / RRP4, S1 domain / Exosome complex component RRP45 / Rrp40, S1 domain / : ...M-phase phosphoprotein 6 / M-phase phosphoprotein 6 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / RRP4, S1 domain / Exosome complex component RRP45 / Rrp40, S1 domain / : / Exosome complex component RRP40, S1 domain / Exosome complex component CSL4, C-terminal / Exosome complex component, N-terminal domain / Exosome complex component Csl4 / Exosome component EXOSC1/CSL4 / Exosome complex exonuclease RRP4 N-terminal region / : / Exosome complex RNA-binding protein 1/RRP40/RRP4 / KH domain / GHMP Kinase, N-terminal domain / K Homology domain, type 1 / Exoribonuclease, phosphorolytic domain 2 / 3' exoribonuclease family, domain 2 / Exoribonuclease, phosphorolytic domain 1 / PNPase/RNase PH domain superfamily / Exoribonuclease, PH domain 2 superfamily / 3' exoribonuclease family, domain 1 / K Homology domain, type 1 / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Ribosomal Protein S8; Chain: A, domain 1 / K Homology domain, type 1 superfamily / Ribosomal Protein S5; domain 2 / Nucleic acid-binding proteins / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold / Beta Barrel / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Exosome complex component RRP43 / Exosome complex component RRP4 / Exosome complex component SKI6 / Exosome complex component MTR3 / Exosome complex component RRP46 / M-phase phosphoprotein 6 homolog / Exosome complex component CSL4 / Exosome complex component RRP45 / Exosome complex component RRP40 / Exosome complex component RRP42
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.20003898551 Å
AuthorsFalk, S. / Ebert, J. / Conti, E.
Funding support Belgium, Germany, 4items
OrganizationGrant numberCountry
European Research Council294371 Belgium
German Research FoundationSFB1035 Germany
German Research FoundationGRK1721 Germany
German Research FoundationCluster of Excellence CIPSM Germany
CitationJournal: Cell Rep / Year: 2017
Title: Mpp6 Incorporation in the Nuclear Exosome Contributes to RNA Channeling through the Mtr4 Helicase.
Authors: Falk, S. / Bonneau, F. / Ebert, J. / Kogel, A. / Conti, E.
History
DepositionJul 26, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 30, 2017Provider: repository / Type: Initial release
Revision 1.1Sep 20, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
m: Exosome complex component CSL4
A: Exosome complex component RRP45
B: Exosome complex component SKI6
C: Exosome complex component RRP43
D: Exosome complex component RRP46
E: Exosome complex component RRP42
F: Exosome complex component MTR3
G: Exosome complex component RRP40
H: Exosome complex component RRP4
I: Exosome complex component CSL4
J: M-phase phosphoprotein 6 homolog
K: Exosome complex component RRP45
L: Exosome complex component SKI6
M: Exosome complex component RRP43
N: Exosome complex component RRP46
O: Exosome complex component RRP42
P: Exosome complex component MTR3
Q: Exosome complex component RRP40
R: Exosome complex component RRP4
S: Exosome complex component CSL4
T: M-phase phosphoprotein 6 homolog
U: Exosome complex component RRP45
V: Exosome complex component SKI6
W: Exosome complex component RRP43
X: Exosome complex component RRP46
Y: Exosome complex component RRP42
Z: Exosome complex component MTR3
a: Exosome complex component RRP40
b: Exosome complex component RRP4
c: Exosome complex component CSL4
d: M-phase phosphoprotein 6 homolog
e: Exosome complex component RRP45
f: Exosome complex component SKI6
g: Exosome complex component RRP43
h: Exosome complex component RRP46
i: Exosome complex component RRP42
j: Exosome complex component MTR3
k: Exosome complex component RRP40
l: Exosome complex component RRP4
n: M-phase phosphoprotein 6 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,211,38253
Polymers1,210,43240
Non-polymers95013
Water2,522140
1
m: Exosome complex component CSL4
e: Exosome complex component RRP45
f: Exosome complex component SKI6
g: Exosome complex component RRP43
h: Exosome complex component RRP46
i: Exosome complex component RRP42
j: Exosome complex component MTR3
k: Exosome complex component RRP40
l: Exosome complex component RRP4
n: M-phase phosphoprotein 6 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)302,86614
Polymers302,60810
Non-polymers2584
Water1267
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area31280 Å2
ΔGint-176 kcal/mol
Surface area89130 Å2
MethodPISA
2
A: Exosome complex component RRP45
B: Exosome complex component SKI6
C: Exosome complex component RRP43
D: Exosome complex component RRP46
E: Exosome complex component RRP42
F: Exosome complex component MTR3
G: Exosome complex component RRP40
H: Exosome complex component RRP4
I: Exosome complex component CSL4
J: M-phase phosphoprotein 6 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)302,84213
Polymers302,60810
Non-polymers2343
Water1267
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area31470 Å2
ΔGint-172 kcal/mol
Surface area88760 Å2
MethodPISA
3
K: Exosome complex component RRP45
L: Exosome complex component SKI6
M: Exosome complex component RRP43
N: Exosome complex component RRP46
O: Exosome complex component RRP42
P: Exosome complex component MTR3
Q: Exosome complex component RRP40
R: Exosome complex component RRP4
S: Exosome complex component CSL4
T: M-phase phosphoprotein 6 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)302,86614
Polymers302,60810
Non-polymers2584
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
U: Exosome complex component RRP45
V: Exosome complex component SKI6
W: Exosome complex component RRP43
X: Exosome complex component RRP46
Y: Exosome complex component RRP42
Z: Exosome complex component MTR3
a: Exosome complex component RRP40
b: Exosome complex component RRP4
c: Exosome complex component CSL4
d: M-phase phosphoprotein 6 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)302,80712
Polymers302,60810
Non-polymers1992
Water1267
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area31160 Å2
ΔGint-153 kcal/mol
Surface area90560 Å2
MethodPISA
Unit cell
Length a, b, c (Å)161.779, 237.430, 201.895
Angle α, β, γ (deg.)90.00, 110.37, 90.00
Int Tables number4
Space group name H-MP1211
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
12
22
32
42
13
23
33
43
14
24
15
25
35
45
16
26
36
46
17
27
37
47
18
28
38
19
29
39
49
110
210
310

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LYSLYSMETMET(chain A and (resseq 3:13 or (resid 14 and (name...AB3 - 2963 - 296
211LYSLYSMETMET(chain K and (resseq 3:13 or (resid 14 and (name...KL3 - 2963 - 296
311LYSLYSMETMET(chain U and (resseq 3:13 or (resid 14 and (name...UV3 - 2963 - 296
411LYSLYSMETMET(chain e and ((resid 3 and (name N or name...eFA3 - 2963 - 296
112ARGARGPHEPHE(chain F and (resseq 8:19 or (resid 20 and (name...FG8 - 208 - 20
122GLNGLNASPASP(chain F and (resseq 8:19 or (resid 20 and (name...FG44 - 14644 - 146
132SERSERMETMET(chain F and (resseq 8:19 or (resid 20 and (name...FG163 - 246163 - 246
212ARGARGPHEPHE(chain P and (resseq 8:19 or (resid 20 and (name...PQ8 - 208 - 20
222GLNGLNASPASP(chain P and (resseq 8:19 or (resid 20 and (name...PQ44 - 14644 - 146
232SERSERMETMET(chain P and (resseq 8:19 or (resid 20 and (name...PQ163 - 246163 - 246
312ARGARGPHEPHE(chain Z and (resseq 8:15 or (resid 16 and (name...ZAA8 - 208 - 20
322GLNGLNASPASP(chain Z and (resseq 8:15 or (resid 16 and (name...ZAA44 - 14644 - 146
332SERSERMETMET(chain Z and (resseq 8:15 or (resid 16 and (name...ZAA163 - 246163 - 246
412ARGARGPHEPHE(chain j and (resseq 8:15 or (resid 16 and (name...jKA8 - 208 - 20
422GLNGLNASPASP(chain j and (resseq 8:15 or (resid 16 and (name...jKA44 - 14644 - 146
432SERSERMETMET(chain j and (resseq 8:15 or (resid 16 and (name...jKA163 - 246163 - 246
113SERSERPROPRO(chain H and (resseq 52:56 or (resid 57 and (name...HI52 - 1449 - 101
123LEULEUARGARG(chain H and (resseq 52:56 or (resid 57 and (name...HI157 - 243114 - 200
133GLNGLNGLNGLN(chain H and (resseq 52:56 or (resid 57 and (name...HI279 - 316236 - 273
143ARGARGMETMET(chain H and (resseq 52:56 or (resid 57 and (name...HI318 - 354275 - 311
213SERSERPROPRO(chain R and (resseq 52:56 or (resid 57 and (name...RS52 - 1449 - 101
223LEULEUARGARG(chain R and (resseq 52:56 or (resid 57 and (name...RS157 - 243114 - 200
233GLNGLNGLNGLN(chain R and (resseq 52:56 or (resid 57 and (name...RS279 - 316236 - 273
243ARGARGMETMET(chain R and (resseq 52:56 or (resid 57 and (name...RS318 - 354275 - 311
313SERSERPROPRO(chain b and (resseq 52:56 or (resid 57 and (name...bCA52 - 1449 - 101
323LEULEUARGARG(chain b and (resseq 52:56 or (resid 57 and (name...bCA157 - 243114 - 200
343GLNGLNGLNGLN(chain b and (resseq 52:56 or (resid 57 and (name...bCA279 - 316236 - 273
353ARGARGMETMET(chain b and (resseq 52:56 or (resid 57 and (name...bCA318 - 354275 - 311
413SERSERPROPRO(chain l and (resseq 52:65 or (resid 66 and (name...lMA52 - 1449 - 101
423LEULEUARGARG(chain l and (resseq 52:65 or (resid 66 and (name...lMA157 - 243114 - 200
433GLNGLNGLNGLN(chain l and (resseq 52:65 or (resid 66 and (name...lMA279 - 316236 - 273
443ARGARGMETMET(chain l and (resseq 52:65 or (resid 66 and (name...lMA318 - 354275 - 311
114ILEILETHRTHRchain TTU109 - 114113 - 118
214ILEILETHRTHRchain ddEA109 - 114113 - 118
115VALVALCYSCYS(chain C and ((resid 21 and (name N or name...CD21 - 4221 - 42
125LYSLYSTHRTHR(chain C and ((resid 21 and (name N or name...CD45 - 9945 - 99
135GLUGLUPROPRO(chain C and ((resid 21 and (name N or name...CD121 - 190121 - 190
145LYSLYSPHEPHE(chain C and ((resid 21 and (name N or name...CD206 - 247206 - 247
155ARGARGGLYGLY(chain C and ((resid 21 and (name N or name...CD256 - 263256 - 263
165ILEILELEULEU(chain C and ((resid 21 and (name N or name...CD274 - 302274 - 302
175ASNASNPHEPHE(chain C and ((resid 21 and (name N or name...CD327 - 392327 - 392
215VALVALCYSCYS(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN21 - 4221 - 42
225LYSLYSTHRTHR(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN45 - 9945 - 99
235GLUGLUPROPRO(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN121 - 190121 - 190
245LYSLYSPHEPHE(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN206 - 247206 - 247
255ARGARGGLYGLY(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN256 - 263256 - 263
265ILEILELEULEU(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN274 - 302274 - 302
275ASNASNPHEPHE(chain M and (resseq 21:43 or resseq 45:69 or (resid...MN327 - 392327 - 392
315VALVALCYSCYS(chain W and ((resid 21 and (name N or name...WX21 - 4221 - 42
325LYSLYSTHRTHR(chain W and ((resid 21 and (name N or name...WX45 - 9945 - 99
335GLUGLUPROPRO(chain W and ((resid 21 and (name N or name...WX121 - 190121 - 190
345LYSLYSPHEPHE(chain W and ((resid 21 and (name N or name...WX206 - 247206 - 247
355ARGARGGLYGLY(chain W and ((resid 21 and (name N or name...WX256 - 263256 - 263
365ILEILELEULEU(chain W and ((resid 21 and (name N or name...WX274 - 302274 - 302
375ASNASNPHEPHE(chain W and ((resid 21 and (name N or name...WX327 - 392327 - 392
415VALVALCYSCYS(chain g and ((resid 21 and (name N or name...gHA21 - 4221 - 42
425LYSLYSTHRTHR(chain g and ((resid 21 and (name N or name...gHA45 - 9945 - 99
435GLUGLUPROPRO(chain g and ((resid 21 and (name N or name...gHA121 - 190121 - 190
445LYSLYSPHEPHE(chain g and ((resid 21 and (name N or name...gHA206 - 247206 - 247
455ARGARGGLYGLY(chain g and ((resid 21 and (name N or name...gHA256 - 263256 - 263
465ILEILELEULEU(chain g and ((resid 21 and (name N or name...gHA274 - 302274 - 302
475ASNASNPHEPHE(chain g and ((resid 21 and (name N or name...gHA327 - 392327 - 392
116SERSERLEULEU(chain D and ((resid 2 and (name N or name...DE2 - 2215 - 224
216SERSERLEULEU(chain N and ((resid 2 and (name N or name...NO2 - 2215 - 224
316SERSERLEULEU(chain X and ((resid 2 and (name N or name...XY2 - 2215 - 224
416SERSERLEULEU(chain h and (resseq 2:10 or (resid 11 and (name...hIA2 - 2215 - 224
117METMETALAALA(chain E and (resseq 1:11 or (resid 12 and (name...EF1 - 1604 - 163
127LEULEULEULEU(chain E and (resseq 1:11 or (resid 12 and (name...EF169 - 244172 - 247
137GLNGLNVALVAL(chain E and (resseq 1:11 or (resid 12 and (name...EF246 - 259249 - 262
147SERSERLEULEU(chain E and (resseq 1:11 or (resid 12 and (name...EF261 - 262264 - 265
217METMETALAALA(chain O and ((resid 1 and (name N or name...OP1 - 1604 - 163
227LEULEULEULEU(chain O and ((resid 1 and (name N or name...OP169 - 244172 - 247
237GLNGLNVALVAL(chain O and ((resid 1 and (name N or name...OP246 - 259249 - 262
247SERSERLEULEU(chain O and ((resid 1 and (name N or name...OP261 - 262264 - 265
317METMETALAALA(chain Y and ((resid 1 and (name N or name...YZ1 - 1604 - 163
327LEULEULEULEU(chain Y and ((resid 1 and (name N or name...YZ169 - 244172 - 247
337GLNGLNVALVAL(chain Y and ((resid 1 and (name N or name...YZ246 - 259249 - 262
347SERSERLEULEU(chain Y and ((resid 1 and (name N or name...YZ261 - 262264 - 265
417METMETALAALA(chain i and ((resid 1 and (name N or name...iJA1 - 1604 - 163
427LEULEULEULEU(chain i and ((resid 1 and (name N or name...iJA169 - 244172 - 247
437GLNGLNVALVAL(chain i and ((resid 1 and (name N or name...iJA246 - 259249 - 262
447SERSERLEULEU(chain i and ((resid 1 and (name N or name...iJA261 - 262264 - 265
118SERSERHISHIS(chain G and (resseq 2:3 or (resid 4 and (name...GH2 - 436 - 47
128GLNGLNASNASN(chain G and (resseq 2:3 or (resid 4 and (name...GH53 - 10457 - 108
138LYSLYSTHRTHR(chain G and (resseq 2:3 or (resid 4 and (name...GH108 - 140112 - 144
148GLYGLYCYSCYS(chain G and (resseq 2:3 or (resid 4 and (name...GH146 - 198150 - 202
158LEULEUILEILE(chain G and (resseq 2:3 or (resid 4 and (name...GH201 - 234205 - 238
218SERSERHISHIS(chain a and ((resid 2 and (name N or name...aBA2 - 436 - 47
228GLNGLNASNASN(chain a and ((resid 2 and (name N or name...aBA53 - 10457 - 108
238LYSLYSTHRTHR(chain a and ((resid 2 and (name N or name...aBA108 - 140112 - 144
248GLYGLYCYSCYS(chain a and ((resid 2 and (name N or name...aBA146 - 198150 - 202
258LEULEUILEILE(chain a and ((resid 2 and (name N or name...aBA201 - 234205 - 238
318SERSERHISHIS(chain k and (resseq 2:3 or (resid 4 and (name...kLA2 - 436 - 47
328GLNGLNASNASN(chain k and (resseq 2:3 or (resid 4 and (name...kLA53 - 10457 - 108
338LYSLYSTHRTHR(chain k and (resseq 2:3 or (resid 4 and (name...kLA108 - 140112 - 144
348GLYGLYCYSCYS(chain k and (resseq 2:3 or (resid 4 and (name...kLA146 - 198150 - 202
358LEULEUILEILE(chain k and (resseq 2:3 or (resid 4 and (name...kLA201 - 234205 - 238
119LEULEUPROPRO(chain B and (resseq 4:9 or (resid 10 and (name...BC4 - 337 - 36
129ALAALALYSLYS(chain B and (resseq 4:9 or (resid 10 and (name...BC35 - 8338 - 86
139ARGARGARGARG(chain B and (resseq 4:9 or (resid 10 and (name...BC96 - 23999 - 242
219LEULEUPROPRO(chain L and ((resid 4 and (name N or name...LM4 - 337 - 36
229ALAALALYSLYS(chain L and ((resid 4 and (name N or name...LM35 - 8338 - 86
239ARGARGARGARG(chain L and ((resid 4 and (name N or name...LM96 - 23999 - 242
319LEULEUPROPRO(chain V and (resseq 4:9 or (resid 10 and (name...VW4 - 337 - 36
329ALAALALYSLYS(chain V and (resseq 4:9 or (resid 10 and (name...VW35 - 8338 - 86
339ARGARGARGARG(chain V and (resseq 4:9 or (resid 10 and (name...VW96 - 23999 - 242
419LEULEUPROPRO(chain f and ((resid 4 and (name N or name...fGA4 - 337 - 36
429ALAALALYSLYS(chain f and ((resid 4 and (name N or name...fGA35 - 8338 - 86
439ARGARGARGARG(chain f and ((resid 4 and (name N or name...fGA96 - 23999 - 242
1110PROPROPROPRO(chain S and (resseq 8:19 or resseq 37:45 or (resid...ST8 - 1911 - 22
1210PROPROARGARG(chain S and (resseq 8:19 or resseq 37:45 or (resid...ST37 - 6540 - 68
1310SERSERPROPRO(chain S and (resseq 8:19 or resseq 37:45 or (resid...ST109 - 112112 - 115
1410ASNASNLYSLYS(chain S and (resseq 8:19 or resseq 37:45 or (resid...ST126 - 161129 - 164
1510THRTHRPROPRO(chain S and (resseq 8:19 or resseq 37:45 or (resid...ST187 - 291190 - 294
2210PROPROPROPRO(chain c and (resseq 8:19 or resseq 37:45 or (resid...cDA8 - 1911 - 22
2310PROPROARGARG(chain c and (resseq 8:19 or resseq 37:45 or (resid...cDA37 - 6540 - 68
2410SERSERPROPRO(chain c and (resseq 8:19 or resseq 37:45 or (resid...cDA109 - 112112 - 115
2510ASNASNLYSLYS(chain c and (resseq 8:19 or resseq 37:45 or (resid...cDA126 - 161129 - 164
2610THRTHRPROPRO(chain c and (resseq 8:19 or resseq 37:45 or (resid...cDA187 - 291190 - 294
3110PROPROPROPRO(chain m and (resseq 8:19 or resseq 37:45 or (resid...mA8 - 1911 - 22
3210PROPROARGARG(chain m and (resseq 8:19 or resseq 37:45 or (resid...mA37 - 6540 - 68
3310SERSERPROPRO(chain m and (resseq 8:19 or resseq 37:45 or (resid...mA109 - 112112 - 115
3410ASNASNLYSLYS(chain m and (resseq 8:19 or resseq 37:45 or (resid...mA126 - 161129 - 164
3510THRTHRPROPRO(chain m and (resseq 8:19 or resseq 37:45 or (resid...mA187 - 291190 - 294

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10

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Components

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Exosome complex component ... , 9 types, 36 molecules mIScAKUeBLVfCMWgDNXhEOYiFPZjGQ...

#1: Protein
Exosome complex component CSL4 / / CEP1 synthetic lethal protein 4


Mass: 31911.594 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: CSL4, SKI4, YNL232W, N1154 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P53859
#2: Protein
Exosome complex component RRP45 / / Ribosomal RNA-processing protein 45


Mass: 34001.859 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RRP45, YDR280W, D9954.1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q05636
#3: Protein
Exosome complex component SKI6 / / Extracellular mutant protein 20 / Ribosomal RNA-processing protein 41 / Superkiller protein 6


Mass: 27892.041 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: SKI6, ECM20, RRP41, YGR195W, G7587 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P46948
#4: Protein
Exosome complex component RRP43 / / Ribosomal RNA-processing protein 43


Mass: 44093.000 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RRP43, YCR035C, YCR35C, YCR522 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P25359
#5: Protein
Exosome complex component RRP46 / / Ribosomal RNA-processing protein 46


Mass: 24659.428 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RRP46, YGR095C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P53256
#6: Protein
Exosome complex component RRP42 / / Ribosomal RNA-processing protein 42


Mass: 29391.516 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RRP42, YDL111C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q12277
#7: Protein
Exosome complex component MTR3 / / mRNA transport regulator 3


Mass: 27573.896 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: MTR3, YGR158C, G6676 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P48240
#8: Protein
Exosome complex component RRP40 / / Ribosomal RNA-processing protein 40


Mass: 26939.688 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RRP40, YOL142W / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q08285
#9: Protein
Exosome complex component RRP4 / / Ribosomal RNA-processing protein 4


Mass: 34603.148 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: RRP4, YHR069C / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P38792

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Protein , 1 types, 4 molecules JTdn

#10: Protein
M-phase phosphoprotein 6 homolog / Exosome-associated RNA-binding protein MPP6


Mass: 21541.881 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: MPP6, YNR024W, N3230 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P53725

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Non-polymers , 4 types, 153 molecules

#11: Chemical
ChemComp-TAM / TRIS(HYDROXYETHYL)AMINOMETHANE / Tris


Mass: 163.215 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C7H17NO3 / Comment: pH buffer*YM
#12: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Cl
#13: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#14: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 140 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 3.68 Å3/Da / Density % sol: 66.57 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: 0.1 M Tris/Mops pH 7.5 30 mM MgCl2, 30 mM CaCl2 30% PEG 8000/Ethylene glycol

-
Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1.25 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 10, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.25 Å / Relative weight: 1
ReflectionResolution: 3.2→151.66 Å / Num. obs: 229497 / % possible obs: 97.7 % / Redundancy: 4.3 % / Biso Wilson estimate: 72.3570397634 Å2 / CC1/2: 0.985 / Rmerge(I) obs: 0.214 / Rpim(I) all: 0.114 / Net I/σ(I): 5.7
Reflection shellResolution: 3.2→3.25 Å / Redundancy: 2.8 % / Rmerge(I) obs: 1.881 / Mean I/σ(I) obs: 0.6 / Num. unique all: 9275 / CC1/2: 0.243 / Rpim(I) all: 1.298 / % possible all: 79.9

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
DIALSdata processing
Aimlessdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5jea

5jea


Resolution: 3.20003898551→80.76 Å / SU ML: 0.520726703071 / Cross valid method: FREE R-VALUE / Phase error: 29.4475207363
RfactorNum. reflection% reflection
Rfree0.26152374451 11365 4.9721137382 %
Rwork0.222533890933 --
obs0.224490359891 229006 95.764203604 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 95.2736983465 Å2
Refinement stepCycle: LAST / Resolution: 3.20003898551→80.76 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms66971 0 53 140 67164
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.23640.4040962650875010.39233541441411017X-RAY DIFFRACTION73.7199180748
3.2364-3.27450.4134288536216000.37903648363411344X-RAY DIFFRACTION77.6997137653
3.2745-3.31440.4059483899126430.37233981951512162X-RAY DIFFRACTION82.3896538412
3.3144-3.35640.396515864736670.37196138240912695X-RAY DIFFRACTION86.2732438017
3.3564-3.40050.3974223748237740.35367580062913029X-RAY DIFFRACTION89.5658944909
3.4005-3.44710.3764997025257780.34053190113913696X-RAY DIFFRACTION92.7761040959
3.4471-3.49640.3642609299197860.32703372577714126X-RAY DIFFRACTION96.4366552415
3.4964-3.54860.3555625386766870.30980335821614602X-RAY DIFFRACTION98.8747332342
3.5486-3.6040.3472204831947840.31017141103114690X-RAY DIFFRACTION99.3834296724
3.604-3.66310.3405750064268260.32314971054114480X-RAY DIFFRACTION99.3057808344
3.6631-3.72630.3377391607768170.30968688001814542X-RAY DIFFRACTION99.2375783421
3.7263-3.7940.3365280942997780.29182553487214555X-RAY DIFFRACTION99.1016028956
3.794-3.8670.2984103069517590.26150613591114585X-RAY DIFFRACTION99.0894413949
3.867-3.94590.2823718308747560.23577329057614605X-RAY DIFFRACTION98.7528126005
3.9459-4.03170.2737592072987130.22353877996814493X-RAY DIFFRACTION98.3888709156
4.0317-4.12550.2514995996317180.20906485609714389X-RAY DIFFRACTION97.6914123125
4.1255-4.22870.2288019233177140.18854453485514667X-RAY DIFFRACTION99.1491007542
4.2287-4.3430.2144329256137570.1745527327714657X-RAY DIFFRACTION99.419504644
4.343-4.47080.1962728816427980.1690096637314609X-RAY DIFFRACTION99.2655112428
4.4708-4.61510.2134429361347090.16386817251614599X-RAY DIFFRACTION99.2994291645
4.6151-4.78010.2044027723878270.15600206714414564X-RAY DIFFRACTION98.9456766313
4.7801-4.97150.1992942846587400.15808122588214390X-RAY DIFFRACTION98.3809090318
4.9715-5.19770.2167710473917210.16492206103514597X-RAY DIFFRACTION98.343605547
5.1977-5.47170.2203005435177340.1734031577614637X-RAY DIFFRACTION99.4757960135
5.4717-5.81450.2429734695267310.1945724237114699X-RAY DIFFRACTION99.5483870968
5.8145-6.26330.2730667367327410.20001130773614644X-RAY DIFFRACTION99.3157317152
6.2633-6.89350.2523321436867440.20566354881214400X-RAY DIFFRACTION98.0003882741
6.8935-7.89050.2258915294687670.18989107360314648X-RAY DIFFRACTION99.5543787135
7.8905-9.93960.1858380990817700.14274023932814500X-RAY DIFFRACTION98.5670023238
9.9396-94.67540.2184152973427870.20773969087914274X-RAY DIFFRACTION97.1426728586
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.324621009222-0.1226392676410.1061174035540.247374873914-0.1996911824620.185157354592-0.4789989413860.8635103795450.3811834771180.762462457289-0.003525680726830.330984258315-1.051988149620.07004271237780.0001202416734051.47146765088-0.05205551541860.06218542938081.504413756440.06051765107191.1987875110477.555322541919.259625543761.6399750588
20.01622067823940.002045179918860.0139531866480.0179486989383-0.004521425002860.00512396425471-0.7901722158-0.5245355228620.222529981477-0.076926324254-0.3623831071330.5724885833330.5669568575280.465782667957-0.0007569442186961.44880421506-0.200795627792-0.363209960231.465453189480.2792266422350.86354497683476.874337756320.236836052280.9748403212
30.1135582040620.0987288486318-0.1358732612340.0993835637221-0.003862448283220.3575990500940.5377901938680.9546955349570.6582414294670.002049902565170.249812380107-0.3595082308550.03566201840521.274814860770.009918916160951.44696506357-0.248405499630.1970694637791.358847036180.2777784775932.3828341416190.290407304617.56307533296.9950256154
40.09948257057020.03477682492580.08498945197770.005129507782830.01269267310320.02841881555340.2572587907460.347954693705-0.6259454615460.7008490050610.3008767721310.3246003817990.656949288170.09227184375929.77964554798E-51.46331808491-0.0112361912153-0.01382698441811.58293966316-0.02475619674571.5206243594680.801753353415.493216209691.9166178972
50.57090578455-0.06367477571750.6028517049250.0644280391419-0.06465286214130.5543925311-0.1701473102040.5862586028220.809972417935-0.2824205345810.266118788007-0.717500997997-1.008613632330.266288616353-2.71926611899E-51.86923566375-0.1842493320870.2480624884521.19803561735-0.1906209531412.4246855593191.425671659423.2610210184105.564180955
62.735712925360.0771872315314-0.2657698749132.56250392167-0.4753605372540.605960663068-0.05309717742550.06920843140670.291302584831-0.111801312181-0.0789186103224-0.2231270368450.0678976652461-0.152961613767-0.000138333574580.6269013997980.06355669465490.01027175299770.530077426138-0.0375394167710.54654270688785.3815201839-78.9475984008110.463613968
71.45009606798-0.7258830350.09174617281880.946197383656-0.6794600760271.55448875363-0.123130630042-0.4324393403980.6741012107080.256258125852-0.107775187145-0.0654457205294-0.048680306114-0.09894632441783.1721929119E-50.7122663403730.145096544025-0.03202990049720.560320564326-0.1219955271580.57421036209280.9034349685-71.3864948827113.358908786
80.0486704235518-0.00346183308546-0.01293644007560.0430309883426-0.03111000818360.17230323686-0.8681601799-0.3239181492021.0686215635-0.636956807061-0.136040653334-0.593470102905-0.7544755106420.65909104352-2.6543428702E-50.670550393229-0.186482835158-0.04566051575720.792753811932-0.03062763287061.3587181000175.0713481785-48.997223693286.0599285196
90.403834558583-0.124533787579-0.02872968303680.0439452941340.04622326363530.1632925313080.0090031537780.6222590488330.058479406887-0.4385893216960.124447563196-0.5085129587390.2395419684280.0929385214342-8.10526030196E-60.93942937642-0.193488027648-0.1608657017130.7201725217460.1105865034540.58512330697267.3692990232-73.978321316173.7033135701
100.620426810548-0.1592067111680.1196508891810.4045091810320.6334083110031.1124467290.123431952922-0.288202967782-0.190021781898-0.00701324266015-0.1717964614420.4578389097370.1330864507270.0887057669552.24528440019E-50.661252614876-0.06891353119-0.1184060861460.5894061528410.0292697270580.80714167936370.9371187208-83.522794094982.427104789
110.0499502004245-0.001078178938250.0182043651454-0.002255171056970.007431365261870.01618947173340.680337154476-1.102721880930.211606947764-0.1921991653550.513323099642-0.3167320170350.137211123302-0.2923033591188.78787962835E-50.775127794246-0.1869040650190.009487074897961.0560642469-0.1797358464250.70505904655289.9155671981-72.859946579580.366912569
121.67563625949-1.01090192008-0.3028197684121.216231921870.3842378510371.53896091857-0.21625753123-0.162762646253-0.00688494752771-0.04056891060310.00896972831218-0.08661860932970.380972654932-0.06800836965917.27829197454E-50.593700636569-0.1253096513830.02815954267810.560726836345-0.01647970404180.44048818539270.5875095257-79.902003162686.9159671967
130.836029397675-0.8268510148340.2807075501790.8937701425680.2521079964681.1681937537-0.3244094781060.188405215030.372510890885-0.314814641620.0899755871137-0.608164750418-0.2357097143240.06248649532244.24739996167E-50.456779973492-0.0117071361605-0.03270684981960.5353268840630.01766992595550.84954156370770.8113895261-64.092613619790.9170512366
140.09706077759730.06792619808390.1365578007070.554575260506-0.2969731127340.268283576484-0.2596620573620.3396272226370.5233679308060.184764486538-0.0171700594474-0.186144251674-0.1914789859040.0102160159185-1.25248676092E-50.5949868889750.0242851966367-0.002853884847650.653433674705-0.01729834426310.73451737283369.9235663876-64.7480396035100.054524139
151.35770283036-0.6508441505590.1723330434271.25572938634-0.7735339276330.562721220708-0.2151446680590.4055840680520.481503013581-0.707050729481-0.3866023235910.382940901475-0.490362056654-0.248884820635-0.0002867753079540.5568706734860.0423935594585-0.03578371154130.751919651830.03614746694560.93692703778859.0557039173-66.917932980982.4252336059
162.12184603122-0.1891879323450.6397537028441.26543144314-0.04853513046332.188606344860.01171726983330.37905319655-0.0868687296421-0.163464794068-0.117213483978-0.24531555853-0.0209315819720.112091211231-1.90414382941E-50.6049729892360.1923126463780.1879194420370.7763829848280.02608584810020.909334227689114.565181837-103.17657396693.8219716671
170.986576750022-0.183945170039-0.3080551660340.295510072270.5905537970681.526788266780.05701074494820.4639767895740.6316732139860.0801905140696-0.0352977975504-0.500905965524-0.4691161970.1794679993863.54769432408E-60.7747420096120.2053853048890.1054736114790.7057645475110.2358250319111.26968715525120.448634464-89.686087536790.2436957721
180.0526606139993-0.349827605797-0.02031616287280.479608676115-0.0214504392692-0.127171669127-0.1951553523330.4187193413690.0769004845953-0.198297331468-0.171612237295-0.862968939999-0.132630468980.287762746742-0.0001884306856660.9281075163230.007292795803680.2943446135150.8336854998560.1875630538341.59265687298121.848067878-76.370807137188.5229496355
191.17446112701-0.752072260090.7818994023111.14433676964-0.1369205747322.037033762870.00456554654180.085112850406-0.2660136591910.0875522925921-0.0382686519572-0.3195898126230.2865683095570.149848947446-4.39149041428E-50.7307044092680.2092614504820.005819851638190.59324859638-0.037821994240.808858212156116.197420565-115.265473834105.922939502
200.3398975356630.438391606484-0.250920538030.492864716679-0.3077681080290.179191893106-0.57566266754-0.2475764198730.8740914685941.174937018920.3543447155910.3218609955060.1744071877550.115711837865-0.00578325842840.9893987195540.098799103175-0.03739432732370.572385332677-0.08285998470570.51126314852788.4329651584-95.3271843375123.870106991
210.299697416336-0.0600995291639-0.2306471383140.346898087777-0.2813152997440.473476266948-1.291857791460.03825864070770.3489904384480.6343558728770.7020606642070.0279799555415-0.5596126146790.488800622356-0.01368753885350.88726473244-0.0248248727629-0.07512172824010.634911174474-0.06534059210550.41943984413195.3747299707-94.1893257014121.161711991
220.1618737051430.260077752883-0.05680078910110.452824274324-0.1139126694730.153087672330.1828098856830.693829195007-0.250366947727-0.4270275545460.05022571326010.675054960968-0.0545374339569-0.1174094609270.001862642776150.829693197452-0.1213645204820.1293147599350.714523484657-0.1569379467160.44274517632784.888749188-109.633269391103.601952097
230.154266527858-0.03621813908060.0507989820270.0578007809671-0.07264758401570.0588204921103-0.08335806111-0.1831809991530.235178587374-0.95083980305-0.441254129725-0.180640364-0.0109969729893-0.0192383477359-2.86683342874E-50.659583768350.1234689911070.08657313718120.5970108248170.1427247294340.49067583032195.0709587875-95.2415622568107.858227257
240.05012236431670.03972627398020.02043055414080.02351359271840.008004609785850.2162880833830.1526099645040.3637776545290.118000151337-0.129119056089-0.279643325659-0.0071239798953-0.0788939748492-0.6871813830721.17842790698E-50.6929763988210.07739104000970.1009594755880.62964216449-0.06410610281350.490238705142100.617886822-102.104599531109.095266708
250.459770742671-0.3106040863930.01122074143290.477023716719-0.4711670715270.735488325317-0.0912762517949-0.002039499828660.03980916656350.2648560140110.02244270191-0.07053972651490.273953215122-0.09097244712384.04613757517E-50.8211604689820.1547437127010.03135913945010.7026526963860.01122413606580.58895682090289.7796099374-105.373129074111.907432906
260.737715407276-0.683737344008-0.1095334084080.6561091579990.3026138325971.07208762434-0.214203578303-0.1027613924820.5829658936220.1765831838740.481599051179-0.6926707277450.5582155726910.3043491286090.01343412822710.7595846680530.18333578723-0.01433531295320.5873766406910.0805472473330.621961211302105.094062934-98.4733778229119.890001269
270.676457663173-0.1652458894830.05606203375990.0455309086922-0.06433108510420.508195809035-0.0632515169364-1.057747654340.759691593560.09748863441570.1298363067480.186226764314-0.1960006791590.2786092869490.01829757689030.6528843134860.0709821289464-0.2559737429010.6428480234390.03368365994820.899236232501113.036069923-98.0668800078119.581318253
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1630.110390564258-0.09938242063240.1506075807650.7507354633610.308795538690.583308292022-0.4705180434260.1013481004450.07387642978210.2356636900770.331763211392-0.04993083319950.258402864481-0.455280620006-0.0001692550526810.8835117018280.0242743136994-0.1143505088430.854803213629-0.02865132249950.68258913720777.9866662041-15.600334114126.047304556
1640.09109642665970.032529441216-0.1119793408370.0291634906131-0.03200644093460.0993294240065-0.782433771334-0.5121127774940.180698119359-0.5301902450080.73943452065-0.3709402292460.336130967059-0.04902987252810.0001674544801190.9554645864090.0464977832681-0.1492512986710.834331365514-0.1129843514211.0594058310386.91623175020.836942724344109.185531077
1651.17110177746-0.501206151560.3251645768590.652905518270.5221699146560.867717737785-0.269012611112-0.337352819340.264541060544-0.108377679720.129910500671-0.484806323417-0.1589028843040.0781594695374-1.57366705991E-50.8666394133520.113320491797-0.1638762503240.608315828035-0.1295062283880.75165077318976.6403776447-7.7785471288113.916310598
1660.825336285596-0.755362882752-0.09787666310820.791625524298-0.1393021971570.271712240194-0.1296330267850.4866272933380.03655679596320.4853291821110.4486200511140.0865935778302-0.3395476522160.0966364766873-3.64566300735E-50.8146490515150.152276035408-0.1822532478480.715887872035-0.1409235195360.6111419650268.9526491613-10.4386599515122.512306339
1670.712043823653-0.5657002984750.100636402690.4641646126590.0906280389730.437104040803-0.077055073903-0.96598372342-1.075856333230.4886499846370.280532474205-0.2154098410680.138729405702-0.1717063769560.0009592501910810.7399228262890.140539438393-0.07077592135570.8416092363080.0538863416890.72374820543255.3616149997-13.0289484275124.156689355
1680.6495631404580.150295364562-0.6400840608350.383124611757-0.120045882880.901491745523-0.0319643108658-0.788525851637-0.1092561450420.6151227773610.1180243933240.355658732139-0.13323062917-0.02487729779370.008394795593541.019119224450.184502722425-0.1187049807340.801147938978-0.1552721571020.61193160148665.615412727-3.40563755901129.915942258
1691.36129356217-0.2776679494110.2018188224761.437151624150.02032135876230.253228607672-0.0382066465660.4902132883710.0799593816068-0.03427200307220.130914008682-0.295797137421-0.0492917822579-0.1668642237168.58760188541E-50.697998924339-0.107737379138-0.07931291660050.837332033186-0.02462937599240.51738414575987.2224633548-20.603992873466.0471652448
1700.28538792816-0.109880505047-0.1574147441990.368534223969-0.06880514946960.820158734284-0.4078792260330.2049019480840.806208373294-0.534545585327-0.05989364261810.6305321694910.0470920125891-0.5965646361050.0001756990996960.708093934304-0.148445922569-0.1857343491571.02157811777-0.0002306918131540.56746755797773.4722442214-34.303285297866.8684810682
1711.804913968450.195204219447-0.1424789011360.720694961624-1.18218266942.37263448958-0.01706203574420.395048040006-0.0413774421205-0.3826085359190.161956992904-0.274439952520.15579965516-0.299034242116-9.44440128865E-50.583091989561-0.0864270197492-0.06351264885380.834019830494-0.04712347909840.55980982695482.0360566291-22.103534583659.2030542747
1720.07315533731620.190677861280.171125741450.2733567644570.1226114666490.0940108872999-0.5563870038890.3782123260190.05441949696290.208629270682-0.266188460548-0.0143685165211-0.4387176525460.143311958984-6.30869414197E-51.004649711120.3473801012140.02354374528431.05178478361-0.1564229352781.0525029048862.6197508739-20.6003086691.7525823653
1732.77595732485-0.879727363357-0.619060810762.460949536250.7499803277583.10698366740.08464223384030.3190793255690.347732403943-0.3067003705290.03104583944440.272769164954-0.46347133364-0.480217451191.23700398311E-50.8163232194830.130066231546-0.120201401480.8206648472050.06444253445480.65207757079369.13743045430.057904928537570.8693103838
1742.942131968511.93284721019-0.5582618394291.32218338661-0.3052185058011.889712000320.622590460614-1.390180308852.088381886330.6301843921460.1461728246290.645423129235-0.775621406817-0.2586369814850.9640871590971.430633062970.615090888463-0.3921724053430.919993394218-1.149286857790.99686955153782.133963420911.5037608152133.684562548
1750.5197718319510.5339813804780.1794230638090.6030034777110.005521476319540.364292810278-0.118944705952-0.167962498529-0.2528575295240.274434113673-0.110638907949-0.796002986735-0.826816980938-0.1064329761962.88856273778E-51.046174063960.0334707547701-0.2736677743241.04418578807-0.3240894426631.3621735319396.45552599382.34012703577124.42529806
1761.05164414747-1.504563265580.1889432626223.190551083740.9361863644691.51305323945-0.1485658873020.1626191401681.52551171062-0.93238613732-0.00211727919837-1.76262649062-1.25384435040.756696315074-0.002022593424080.890595359434-0.291869828135-0.1503172775920.93374216801-0.4911530956612.85617242121110.0088877965.22478938185115.316955381
1770.849726826271-0.632555217926-0.7511133649450.7338338544320.3242109397720.772433945644-0.239560019048-0.650455303171.024363196910.7963636211360.192545147355-0.989918396491-0.03425559756690.1403162297656.70914560485E-50.9827026221590.0182368432504-0.4743701904011.28983437869-0.2171598865361.35445596345107.437200802-11.3654562843132.686812563
1780.1529927292840.236169040483-0.1515022876771.664197302450.2418766019840.707940843223-0.213203383987-0.09916688577390.0893099877090.187499895818-0.177586300356-0.979443883238-0.07438383927180.281105764167-8.19447191494E-50.644834537066-0.201684633624-0.0953384804370.7407416958110.06476578009611.17472210211120.389729675-16.449990414284.8563822419
1791.26409371234-0.0616717563376-0.1422525098630.8686876987210.232638400660.7168869251390.0678575408634-0.3671422475370.7852691083940.104217373355-0.176268570710.357501264485-0.7495940607110.100600006662-0.002091831119770.855656524991-0.288892693815-0.1359081598950.7506699874390.08428999293011.20402430695112.7139201655.6704059944377.6725638845
1802.248616871850.9900285088311.327809250470.961199169701-0.5664871265142.6445693724-0.3944012091371.115410355220.101706867955-0.6948370126310.126315832699-0.225992579191-0.07402528192550.570758139176-0.0004332955543620.888615643178-0.2537777297670.1538730266041.104318537720.0644512324790.936601399481113.78258096-7.4835327247558.3055944123
1810.0726933212752-0.1096460750180.03100689262530.150588560164-0.01463600233460.0449282807714-0.100537392821-0.793564740949-0.1115843157770.4682482147950.4583772120190.002406409470390.09087763834840.5964776865940.003342723258041.20936616882-0.284982244414-0.4693418601591.36284450143-0.1524873578991.03206683937114.728636672-2.98829424463135.002444194
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'm' and (resid 8 through 109 )
2X-RAY DIFFRACTION2chain 'm' and (resid 110 through 131 )
3X-RAY DIFFRACTION3chain 'm' and (resid 132 through 159 )
4X-RAY DIFFRACTION4chain 'm' and (resid 160 through 201 )
5X-RAY DIFFRACTION5chain 'm' and (resid 202 through 291 )
6X-RAY DIFFRACTION6chain 'A' and (resid 3 through 161 )
7X-RAY DIFFRACTION7chain 'A' and (resid 162 through 297 )
8X-RAY DIFFRACTION8chain 'B' and (resid 3 through 22 )
9X-RAY DIFFRACTION9chain 'B' and (resid 23 through 37 )
10X-RAY DIFFRACTION10chain 'B' and (resid 38 through 78 )
11X-RAY DIFFRACTION11chain 'B' and (resid 79 through 92 )
12X-RAY DIFFRACTION12chain 'B' and (resid 93 through 149 )
13X-RAY DIFFRACTION13chain 'B' and (resid 150 through 190 )
14X-RAY DIFFRACTION14chain 'B' and (resid 191 through 209 )
15X-RAY DIFFRACTION15chain 'B' and (resid 210 through 244 )
16X-RAY DIFFRACTION16chain 'C' and (resid 16 through 175 )
17X-RAY DIFFRACTION17chain 'C' and (resid 176 through 240 )
18X-RAY DIFFRACTION18chain 'C' and (resid 241 through 291 )
19X-RAY DIFFRACTION19chain 'C' and (resid 292 through 393 )
20X-RAY DIFFRACTION20chain 'D' and (resid 2 through 14 )
21X-RAY DIFFRACTION21chain 'D' and (resid 15 through 31 )
22X-RAY DIFFRACTION22chain 'D' and (resid 32 through 45 )
23X-RAY DIFFRACTION23chain 'D' and (resid 46 through 60 )
24X-RAY DIFFRACTION24chain 'D' and (resid 61 through 77 )
25X-RAY DIFFRACTION25chain 'D' and (resid 78 through 96 )
26X-RAY DIFFRACTION26chain 'D' and (resid 97 through 150 )
27X-RAY DIFFRACTION27chain 'D' and (resid 151 through 167 )
28X-RAY DIFFRACTION28chain 'D' and (resid 168 through 187 )
29X-RAY DIFFRACTION29chain 'D' and (resid 188 through 222 )
30X-RAY DIFFRACTION30chain 'E' and (resid 0 through 16 )
31X-RAY DIFFRACTION31chain 'E' and (resid 17 through 103 )
32X-RAY DIFFRACTION32chain 'E' and (resid 104 through 119 )
33X-RAY DIFFRACTION33chain 'E' and (resid 120 through 176 )
34X-RAY DIFFRACTION34chain 'E' and (resid 177 through 202 )
35X-RAY DIFFRACTION35chain 'E' and (resid 203 through 263 )
36X-RAY DIFFRACTION36chain 'F' and (resid 6 through 44 )
37X-RAY DIFFRACTION37chain 'F' and (resid 45 through 247 )
38X-RAY DIFFRACTION38chain 'G' and (resid 0 through 18 )
39X-RAY DIFFRACTION39chain 'G' and (resid 19 through 94 )
40X-RAY DIFFRACTION40chain 'G' and (resid 95 through 125 )
41X-RAY DIFFRACTION41chain 'G' and (resid 126 through 219 )
42X-RAY DIFFRACTION42chain 'G' and (resid 220 through 237 )
43X-RAY DIFFRACTION43chain 'H' and (resid 52 through 99 )
44X-RAY DIFFRACTION44chain 'H' and (resid 100 through 127 )
45X-RAY DIFFRACTION45chain 'H' and (resid 128 through 161 )
46X-RAY DIFFRACTION46chain 'H' and (resid 162 through 242 )
47X-RAY DIFFRACTION47chain 'H' and (resid 243 through 290 )
48X-RAY DIFFRACTION48chain 'H' and (resid 291 through 355 )
49X-RAY DIFFRACTION49chain 'I' and (resid 11 through 37 )
50X-RAY DIFFRACTION50chain 'I' and (resid 38 through 58 )
51X-RAY DIFFRACTION51chain 'I' and (resid 59 through 137 )
52X-RAY DIFFRACTION52chain 'I' and (resid 138 through 149 )
53X-RAY DIFFRACTION53chain 'I' and (resid 150 through 194 )
54X-RAY DIFFRACTION54chain 'I' and (resid 195 through 209 )
55X-RAY DIFFRACTION55chain 'I' and (resid 210 through 236 )
56X-RAY DIFFRACTION56chain 'I' and (resid 237 through 261 )
57X-RAY DIFFRACTION57chain 'I' and (resid 262 through 290 )
58X-RAY DIFFRACTION58chain 'J' and (resid 90 through 94 )
59X-RAY DIFFRACTION59chain 'J' and (resid 95 through 99 )
60X-RAY DIFFRACTION60chain 'J' and (resid 108 through 117 )
61X-RAY DIFFRACTION61chain 'K' and (resid 3 through 161 )
62X-RAY DIFFRACTION62chain 'K' and (resid 162 through 176 )
63X-RAY DIFFRACTION63chain 'K' and (resid 177 through 224 )
64X-RAY DIFFRACTION64chain 'K' and (resid 225 through 297 )
65X-RAY DIFFRACTION65chain 'L' and (resid 2 through 29 )
66X-RAY DIFFRACTION66chain 'L' and (resid 30 through 78 )
67X-RAY DIFFRACTION67chain 'L' and (resid 79 through 109 )
68X-RAY DIFFRACTION68chain 'L' and (resid 110 through 166 )
69X-RAY DIFFRACTION69chain 'L' and (resid 167 through 241 )
70X-RAY DIFFRACTION70chain 'M' and (resid 19 through 51 )
71X-RAY DIFFRACTION71chain 'M' and (resid 52 through 175 )
72X-RAY DIFFRACTION72chain 'M' and (resid 176 through 240 )
73X-RAY DIFFRACTION73chain 'M' and (resid 241 through 264 )
74X-RAY DIFFRACTION74chain 'M' and (resid 272 through 335 )
75X-RAY DIFFRACTION75chain 'M' and (resid 336 through 370 )
76X-RAY DIFFRACTION76chain 'M' and (resid 371 through 393 )
77X-RAY DIFFRACTION77chain 'N' and (resid 2 through 31 )
78X-RAY DIFFRACTION78chain 'N' and (resid 32 through 45 )
79X-RAY DIFFRACTION79chain 'N' and (resid 46 through 85 )
80X-RAY DIFFRACTION80chain 'N' and (resid 86 through 105 )
81X-RAY DIFFRACTION81chain 'N' and (resid 106 through 139 )
82X-RAY DIFFRACTION82chain 'N' and (resid 140 through 150 )
83X-RAY DIFFRACTION83chain 'N' and (resid 151 through 223 )
84X-RAY DIFFRACTION84chain 'O' and (resid 0 through 15 )
85X-RAY DIFFRACTION85chain 'O' and (resid 16 through 82 )
86X-RAY DIFFRACTION86chain 'O' and (resid 83 through 102 )
87X-RAY DIFFRACTION87chain 'O' and (resid 103 through 119 )
88X-RAY DIFFRACTION88chain 'O' and (resid 120 through 199 )
89X-RAY DIFFRACTION89chain 'O' and (resid 200 through 265 )
90X-RAY DIFFRACTION90chain 'P' and (resid 5 through 44 )
91X-RAY DIFFRACTION91chain 'P' and (resid 45 through 247 )
92X-RAY DIFFRACTION92chain 'Q' and (resid 2 through 24 )
93X-RAY DIFFRACTION93chain 'Q' and (resid 25 through 79 )
94X-RAY DIFFRACTION94chain 'Q' and (resid 80 through 125 )
95X-RAY DIFFRACTION95chain 'Q' and (resid 126 through 140 )
96X-RAY DIFFRACTION96chain 'Q' and (resid 141 through 168 )
97X-RAY DIFFRACTION97chain 'Q' and (resid 169 through 200 )
98X-RAY DIFFRACTION98chain 'Q' and (resid 201 through 235 )
99X-RAY DIFFRACTION99chain 'R' and (resid 50 through 99 )
100X-RAY DIFFRACTION100chain 'R' and (resid 100 through 127 )
101X-RAY DIFFRACTION101chain 'R' and (resid 128 through 158 )
102X-RAY DIFFRACTION102chain 'R' and (resid 159 through 355 )
103X-RAY DIFFRACTION103chain 'S' and (resid 1 through 111 )
104X-RAY DIFFRACTION104chain 'S' and (resid 112 through 291 )
105X-RAY DIFFRACTION105chain 'T' and (resid 109 through 115 )
106X-RAY DIFFRACTION106chain 'U' and (resid 3 through 161 )
107X-RAY DIFFRACTION107chain 'U' and (resid 162 through 201 )
108X-RAY DIFFRACTION108chain 'U' and (resid 202 through 224 )
109X-RAY DIFFRACTION109chain 'U' and (resid 225 through 297 )
110X-RAY DIFFRACTION110chain 'V' and (resid 3 through 90 )
111X-RAY DIFFRACTION111chain 'V' and (resid 91 through 109 )
112X-RAY DIFFRACTION112chain 'V' and (resid 110 through 166 )
113X-RAY DIFFRACTION113chain 'V' and (resid 167 through 243 )
114X-RAY DIFFRACTION114chain 'W' and (resid 15 through 240 )
115X-RAY DIFFRACTION115chain 'W' and (resid 241 through 301 )
116X-RAY DIFFRACTION116chain 'W' and (resid 302 through 394 )
117X-RAY DIFFRACTION117chain 'X' and (resid 1 through 14 )
118X-RAY DIFFRACTION118chain 'X' and (resid 15 through 31 )
119X-RAY DIFFRACTION119chain 'X' and (resid 32 through 45 )
120X-RAY DIFFRACTION120chain 'X' and (resid 46 through 96 )
121X-RAY DIFFRACTION121chain 'X' and (resid 97 through 139 )
122X-RAY DIFFRACTION122chain 'X' and (resid 140 through 150 )
123X-RAY DIFFRACTION123chain 'X' and (resid 151 through 167 )
124X-RAY DIFFRACTION124chain 'X' and (resid 168 through 223 )
125X-RAY DIFFRACTION125chain 'Y' and (resid 1 through 38 )
126X-RAY DIFFRACTION126chain 'Y' and (resid 39 through 82 )
127X-RAY DIFFRACTION127chain 'Y' and (resid 83 through 103 )
128X-RAY DIFFRACTION128chain 'Y' and (resid 104 through 132 )
129X-RAY DIFFRACTION129chain 'Y' and (resid 133 through 185 )
130X-RAY DIFFRACTION130chain 'Y' and (resid 186 through 265 )
131X-RAY DIFFRACTION131chain 'Z' and (resid 5 through 44 )
132X-RAY DIFFRACTION132chain 'Z' and (resid 45 through 247 )
133X-RAY DIFFRACTION133chain 'a' and (resid 1 through 18 )
134X-RAY DIFFRACTION134chain 'a' and (resid 19 through 33 )
135X-RAY DIFFRACTION135chain 'a' and (resid 34 through 94 )
136X-RAY DIFFRACTION136chain 'a' and (resid 95 through 125 )
137X-RAY DIFFRACTION137chain 'a' and (resid 126 through 140 )
138X-RAY DIFFRACTION138chain 'a' and (resid 141 through 153 )
139X-RAY DIFFRACTION139chain 'a' and (resid 154 through 168 )
140X-RAY DIFFRACTION140chain 'a' and (resid 169 through 188 )
141X-RAY DIFFRACTION141chain 'a' and (resid 189 through 200 )
142X-RAY DIFFRACTION142chain 'a' and (resid 201 through 235 )
143X-RAY DIFFRACTION143chain 'b' and (resid 51 through 140 )
144X-RAY DIFFRACTION144chain 'b' and (resid 141 through 355 )
145X-RAY DIFFRACTION145chain 'c' and (resid 1 through 40 )
146X-RAY DIFFRACTION146chain 'c' and (resid 41 through 111 )
147X-RAY DIFFRACTION147chain 'c' and (resid 112 through 137 )
148X-RAY DIFFRACTION148chain 'c' and (resid 138 through 190 )
149X-RAY DIFFRACTION149chain 'c' and (resid 191 through 201 )
150X-RAY DIFFRACTION150chain 'c' and (resid 202 through 276 )
151X-RAY DIFFRACTION151chain 'c' and (resid 277 through 291 )
152X-RAY DIFFRACTION152chain 'd' and (resid 109 through 115 )
153X-RAY DIFFRACTION153chain 'e' and (resid 3 through 161 )
154X-RAY DIFFRACTION154chain 'e' and (resid 162 through 240 )
155X-RAY DIFFRACTION155chain 'e' and (resid 241 through 297 )
156X-RAY DIFFRACTION156chain 'f' and (resid 3 through 78 )
157X-RAY DIFFRACTION157chain 'f' and (resid 79 through 109 )
158X-RAY DIFFRACTION158chain 'f' and (resid 110 through 166 )
159X-RAY DIFFRACTION159chain 'f' and (resid 167 through 244 )
160X-RAY DIFFRACTION160chain 'g' and (resid 14 through 226 )
161X-RAY DIFFRACTION161chain 'g' and (resid 227 through 291 )
162X-RAY DIFFRACTION162chain 'g' and (resid 292 through 393 )
163X-RAY DIFFRACTION163chain 'h' and (resid 1 through 31 )
164X-RAY DIFFRACTION164chain 'h' and (resid 32 through 46 )
165X-RAY DIFFRACTION165chain 'h' and (resid 47 through 96 )
166X-RAY DIFFRACTION166chain 'h' and (resid 97 through 139 )
167X-RAY DIFFRACTION167chain 'h' and (resid 140 through 167 )
168X-RAY DIFFRACTION168chain 'h' and (resid 168 through 223 )
169X-RAY DIFFRACTION169chain 'i' and (resid 1 through 103 )
170X-RAY DIFFRACTION170chain 'i' and (resid 104 through 132 )
171X-RAY DIFFRACTION171chain 'i' and (resid 133 through 265 )
172X-RAY DIFFRACTION172chain 'j' and (resid 5 through 44 )
173X-RAY DIFFRACTION173chain 'j' and (resid 45 through 247 )
174X-RAY DIFFRACTION174chain 'k' and (resid 2 through 33 )
175X-RAY DIFFRACTION175chain 'k' and (resid 34 through 94 )
176X-RAY DIFFRACTION176chain 'k' and (resid 95 through 135 )
177X-RAY DIFFRACTION177chain 'k' and (resid 136 through 235 )
178X-RAY DIFFRACTION178chain 'l' and (resid 52 through 140 )
179X-RAY DIFFRACTION179chain 'l' and (resid 141 through 196 )
180X-RAY DIFFRACTION180chain 'l' and (resid 197 through 355 )
181X-RAY DIFFRACTION181chain 'n' and (resid 108 through 117 )

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