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- PDB-5o2i: An efficient setup for fixed-target, time-resolved serial crystal... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5o2i | ||||||
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Title | An efficient setup for fixed-target, time-resolved serial crystallography with optical excitation | ||||||
![]() | Fluoroacetate dehalogenase![]() | ||||||
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Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schulz, E.C. / Mueller-Werkmeister, H. / Mehrabi, P. / Pai, E.F. / Miller, R.J.D. | ||||||
![]() | ![]() Title: An efficient setup for fixed-target, time-resolved serial crystallography with optical excitation Authors: Schulz, E.C. / Mueller-Werkmeister, H. / Mehrabi, P. / Pai, E.F. / Miller, R.J.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 128 KB | Display | ![]() |
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PDB format | ![]() | 104.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 291.5 KB | Display | ![]() |
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Full document | ![]() | 293 KB | Display | |
Data in XML | ![]() | 24.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3r3uS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 34073.660 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC BAA-98 / CGA009 / Gene: RPA1163 / Production host: ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.42 % |
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Crystal grow![]() | Temperature: 293 K / Method: batch mode / pH: 8.5 Details: 18-20 % (w/v) PEG3350, 200 mM CaCl2, 100 mM Tris-HCl pH 8.5 |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 30, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→83.62 Å / Num. obs: 35399 / % possible obs: 99.55 % / Redundancy: 77.15 % / Net I/σ(I): 1.39 |
Reflection shell | Resolution: 2→2.03 Å |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3R3U Resolution: 2→83.595 Å / SU ML: 0.33 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 24.68 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→83.595 Å
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Refine LS restraints |
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LS refinement shell |
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