- PDB-5nz2: Twist and induce: Dissecting the link between the enzymatic activ... -
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基本情報
登録情報
データベース: PDB / ID: 5nz2
タイトル
Twist and induce: Dissecting the link between the enzymatic activity and the SaPI inducing capacity of the phage 80 dUTPase. D95E mutant from dUTPase 80alpha phage.
要素
DUTPase
キーワード
HYDROLASE / dUTPase / 80 phage / S.aureus
機能・相同性
機能・相同性情報
dUTP catabolic process / dUMP biosynthetic process / dUTP diphosphatase / dUTP diphosphatase activity / magnesium ion binding 類似検索 - 分子機能
分子量: 19128.695 Da / 分子数: 1 / 変異: D95E / 由来タイプ: 組換発現 詳細: C-terminal not present in the strcutre although nucleotide is placed in the active center. Magnesium atom is not coordinated by E95. 由来: (組換発現) Staphylococcus phage 80alpha (ファージ) プラスミド: pet28a / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: A4ZF98
解像度: 2.85→38.99 Å / Cor.coef. Fo:Fc: 0.914 / Cor.coef. Fo:Fc free: 0.869 / SU B: 20.197 / SU ML: 0.367 / 交差検証法: THROUGHOUT / ESU R Free: 0.413 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS