- PDB-5nvd: Crystal structure of hexameric CBS-CP12 protein from bloom-formin... -
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基本情報
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データベース: PDB / ID: 5nvd
タイトル
Crystal structure of hexameric CBS-CP12 protein from bloom-forming cyanobacteria at 2.5 A resolution in P6322 crystal form
要素
CBS-CP12
キーワード
PHOTOSYNTHESIS / Cystathionine beta synthase domain / fusion protein / redox-regulation of photosynthesis
機能・相同性
CP12 domain / CP12 domain / CP12 / CBS domain superfamily / Domain in cystathionine beta-synthase and other proteins. / CBS domain / CBS domain / CBS domain profile. / Similar to tr
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: Protein at 6.08 mg/ml in 50 mM Bicine/KOH pH 7.8, 40 mM KCl, 0.132 mM AMP solution was mixed with reservoir solution containing 0.15 M KSCN, 0.1 M HEPES pH 7.0 and 18% PEG3350 containing 0.1 ...詳細: Protein at 6.08 mg/ml in 50 mM Bicine/KOH pH 7.8, 40 mM KCl, 0.132 mM AMP solution was mixed with reservoir solution containing 0.15 M KSCN, 0.1 M HEPES pH 7.0 and 18% PEG3350 containing 0.1 mM Guanidine hydrochloride. Cryoprotector - reservoir solution with 25% ethylene glycol.
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回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: PETRA III, EMBL c/o DESY / ビームライン: P13 (MX1) / 波長: 0.9184 Å
解像度: 2.5→85.07 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.937 / SU B: 20.157 / SU ML: 0.205 / 交差検証法: THROUGHOUT / ESU R: 0.284 / ESU R Free: 0.223 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS