- PDB-5mj1: Extracellular domain of human CD83 - rhombohedral crystal form -
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基本情報
登録情報
データベース: PDB / ID: 5mj1
タイトル
Extracellular domain of human CD83 - rhombohedral crystal form
要素
CD83 antigen
キーワード
IMMUNE SYSTEM / Dendritic cell / receptor / immunoglobulin
機能・相同性
機能・相同性情報
positive regulation of CD4-positive, alpha-beta T cell differentiation / negative regulation of interleukin-4 production / CD4-positive, alpha-beta T cell differentiation / positive regulation of interleukin-10 production / humoral immune response / positive regulation of interleukin-2 production / response to organic cyclic compound / defense response / external side of plasma membrane / signal transduction / plasma membrane 類似検索 - 分子機能
CD83antigen / hCD83 / B-cell activation protein / Cell surface protein HB15
分子量: 12766.979 Da / 分子数: 1 / 変異: C27S, C100S, C129S / 由来タイプ: 組換発現 詳細: The first four residues (GSPG) are non-native residues of the linker which remain after the GST-tag was cleaved off. The third residue (P) was modeled as alanine due to missing electron ...詳細: The first four residues (GSPG) are non-native residues of the linker which remain after the GST-tag was cleaved off. The third residue (P) was modeled as alanine due to missing electron density. The first and last two residues as well as the central region were not visible in the electron density maps. 由来: (組換発現) Homo sapiens (ヒト) / Cell: Dendritic cells / 遺伝子: CD83 / プラスミド: pGEX-2T / 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' / 参照: UniProt: Q01151
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 0.2 microliter protein (39 mg/ml in ultrapure water) mixed with 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) was equilibrated against 70 microliter of ...詳細: 0.2 microliter protein (39 mg/ml in ultrapure water) mixed with 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) was equilibrated against 70 microliter of reservoir solution. Crystals appeared after ~ 13 months