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- PDB-5lz3: Crystal structure of human ACBD3 GOLD domain in complex with 3A p... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5lz3 | ||||||
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Title | Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus A | ||||||
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![]() | ANTIVIRAL PROTEIN / ACBD3 / GOLD / 3A / Aichivirus | ||||||
Function / homology | ![]() fatty-acyl-CoA binding / steroid biosynthetic process / Golgi Associated Vesicle Biogenesis / host cell Golgi membrane / protein kinase A regulatory subunit binding / symbiont-mediated suppression of host mRNA export from nucleus / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / protein complex oligomerization ...fatty-acyl-CoA binding / steroid biosynthetic process / Golgi Associated Vesicle Biogenesis / host cell Golgi membrane / protein kinase A regulatory subunit binding / symbiont-mediated suppression of host mRNA export from nucleus / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / protein complex oligomerization / monoatomic ion channel activity / symbiont-mediated suppression of host gene expression / RNA helicase activity / RNA helicase / symbiont entry into host cell / RNA-directed RNA polymerase / viral RNA genome replication / Golgi membrane / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / virion attachment to host cell / structural molecule activity / Golgi apparatus / ATP hydrolysis activity / mitochondrion / proteolysis / RNA binding / ATP binding / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Klima, M. / Boura, E. | ||||||
![]() | ![]() Title: Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein. Authors: Klima, M. / Chalupska, D. / Rozycki, B. / Humpolickova, J. / Rezabkova, L. / Silhan, J. / Baumlova, A. / Dubankova, A. / Boura, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 46.6 KB | Display | ![]() |
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PDB format | ![]() | 31 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 414.4 KB | Display | ![]() |
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Full document | ![]() | 414.7 KB | Display | |
Data in XML | ![]() | 7.6 KB | Display | |
Data in CIF | ![]() | 9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5lz1SC ![]() 5lz6C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19261.217 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 6612.464 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.67 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 100mM MOPS/HEPES pH 7.5, 12.5% w/v PEG 1.000, 12.5% w/v PEG 3.350, 12.5% v/v MPD, 20mM D-glucose/D-mannose/D-galactose/L-fucose/D-xylose/N-acetyl-D-glucosamine |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: DECTRIS PILATUS 200K / Detector: PIXEL / Date: Nov 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54187 Å / Relative weight: 1 |
Reflection | Resolution: 3→26.36 Å / Num. obs: 5185 / % possible obs: 95.26 % / Redundancy: 4.1 % / Biso Wilson estimate: 51.86 Å2 / CC1/2: 0.981 / Rmerge(I) obs: 0.2025 / Rsym value: 0.2025 / Net I/σ(I): 7.9 / Num. measured all: 21415 |
Reflection shell | Resolution: 3→3.107 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.6965 / Mean I/σ(I) obs: 1.68 / CC1/2: 0.691 / % possible all: 96.55 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5LZ1 Resolution: 3→26.36 Å / SU ML: 0.33 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.16
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→26.36 Å
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Refine LS restraints |
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LS refinement shell |
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