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- PDB-5lj6: Structure of Aggregatibacter actinomycetemcomitans MacB bound to ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5lj6 | ||||||
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Title | Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P6522) | ||||||
![]() | Macrolide export ATP-binding/permease protein MacB | ||||||
![]() | TRANSPORT PROTEIN / Membrane Protein ABC Transporter | ||||||
Function / homology | ![]() Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / transmembrane transporter activity / response to antibiotic / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Crow, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily. Authors: Crow, A. / Greene, N.P. / Kaplan, E. / Koronakis, V. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 234.3 KB | Display | ![]() |
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PDB format | ![]() | 188.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 676.9 KB | Display | ![]() |
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Full document | ![]() | 687.6 KB | Display | |
Data in XML | ![]() | 22.6 KB | Display | |
Data in CIF | ![]() | 30.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5lilC ![]() 5lj7C ![]() 5lj8C ![]() 5lj9C ![]() 5ljaC ![]() 5naaC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 72114.703 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: macB / Production host: ![]() ![]() References: UniProt: Q2EHL8, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to catalyse transmembrane movement of substances |
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#2: Chemical | ChemComp-ATP / |
#3: Chemical | ChemComp-MG / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.4 Å3/Da / Density % sol: 72.07 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, sitting drop Details: PEG 400, Sodium Citrate, Magnesium Chloride, ATPyS, Dodecylmaltopyranoside |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 22, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97943 Å / Relative weight: 1 |
Reflection | Resolution: 3.9→61.56 Å / Num. obs: 21959 / % possible obs: 97.9 % / Redundancy: 13.8 % / Rsym value: 0.103 / Net I/σ(I): 10.3 |
Reflection shell | Highest resolution: 3.9 Å / Rsym value: 2.466 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Higher resolution MacB structure in space group P21 Resolution: 3.9→59.785 Å / SU ML: 0.63 / Cross valid method: FREE R-VALUE / σ(F): 0.08 / Phase error: 34.9
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.9→59.785 Å
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Refine LS restraints |
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LS refinement shell |
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