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- PDB-5la0: The mechanism by which arabinoxylanases can recognise highly deco... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5la0 | ||||||
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Title | The mechanism by which arabinoxylanases can recognise highly decorated xylans | ||||||
![]() | Carbohydrate binding family 6 | ||||||
![]() | HYDROLASE / Arabinoxylanase Glycoside hydrolase Carbohydrate binding module Arabinose Clostridium thermocellum Cellulosome | ||||||
Function / homology | ![]() cellulose catabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds / carbohydrate binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Basle, A. / Labourel, A. / Cuskin, F. / Jackson, A. / Crouch, L. / Rogowski, A. / Gilbert, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans. Authors: Labourel, A. / Crouch, L.I. / Bras, J.L. / Jackson, A. / Rogowski, A. / Gray, J. / Yadav, M.P. / Henrissat, B. / Fontes, C.M. / Gilbert, H.J. / Najmudin, S. / Basle, A. / Cuskin, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 230.8 KB | Display | ![]() |
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PDB format | ![]() | 181.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.9 KB | Display | ![]() |
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Full document | ![]() | 448.3 KB | Display | |
Data in XML | ![]() | 24.1 KB | Display | |
Data in CIF | ![]() | 38.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5g56C ![]() 5la1C ![]() 5la2C ![]() 5ak1 S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54157.688 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Cther_1146 / Production host: ![]() ![]() | ||||
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#2: Chemical | ChemComp-SO4 / | ||||
#3: Chemical | #4: Sugar | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.7 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion Details: 1 M ammonium sulphate, 0.1 M Bis- Tris, 1 % PEG 3350 pH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 16, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9173 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→44.85 Å / Num. obs: 63523 / % possible obs: 98.5 % / Observed criterion σ(I): 1.5 / Redundancy: 3.5 % / CC1/2: 0.998 / Rmerge(I) obs: 0.067 / Net I/σ(I): 13 |
Reflection shell | Resolution: 1.65→1.68 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.651 / Mean I/σ(I) obs: 1.6 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5AK1 ![]() 5ak1 Resolution: 1.65→44.85 Å / Cor.coef. Fo:Fc: 0.979 / Cor.coef. Fo:Fc free: 0.961 / SU B: 4.129 / SU ML: 0.059 / Cross valid method: THROUGHOUT / ESU R: 0.095 / ESU R Free: 0.077 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.594 Å2
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Refinement step | Cycle: 1 / Resolution: 1.65→44.85 Å
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Refine LS restraints |
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