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- PDB-5klb: Crystal structure of the CavAb voltage-gated calcium channel(wild... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5klb | |||||||||
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Title | Crystal structure of the CavAb voltage-gated calcium channel(wild-type, 2.7A) | |||||||||
![]() | Ion transport protein | |||||||||
![]() | TRANSPORT PROTEIN / Voltage-gated Calcium Channel | |||||||||
Function / homology | ![]() voltage-gated sodium channel complex / membrane depolarization during action potential / voltage-gated sodium channel activity / identical protein binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Tang, L. / Gamal EL-Din, T.M. / Swanson, T.M. / Pryde, D.C. / Scheuer, T. / Zheng, N. / Catterall, W.A. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for inhibition of a voltage-gated Ca(2+) channel by Ca(2+) antagonist drugs. Authors: Tang, L. / El-Din, T.M. / Swanson, T.M. / Pryde, D.C. / Scheuer, T. / Zheng, N. / Catterall, W.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 251.3 KB | Display | ![]() |
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PDB format | ![]() | 205.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 6 MB | Display | ![]() |
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Full document | ![]() | 6.2 MB | Display | |
Data in XML | ![]() | 60.7 KB | Display | |
Data in CIF | ![]() | 72.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5klgC ![]() 5klsC ![]() 5kmdC ![]() 5kmfC ![]() 5kmhC ![]() 4ms2S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33112.000 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: RM4018 / Gene: Abu_1752 / Plasmid: pFASTBAC DUAL / Production host: ![]() #2: Chemical | ChemComp-MC3 / #3: Chemical | #4: Chemical | ChemComp-CPS / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5 Details: CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate PH range: 4.7-5.3 |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 10, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→30 Å / Num. obs: 770488 / % possible obs: 92.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 10.1 % / CC1/2: 0.998 / Rmerge(I) obs: 0.114 / Net I/σ(I): 13.4 |
Reflection shell | Resolution: 2.7→2.85 Å / Redundancy: 9.8 % / Rmerge(I) obs: 0.984 / Mean I/σ(I) obs: 2.4 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4MS2 Resolution: 2.7→29.932 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 0.08 / Phase error: 27.65 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→29.932 Å
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Refine LS restraints |
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LS refinement shell |
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