Component-ID: _ / Beg auth comp-ID: ILE / Beg label comp-ID: ILE / End auth comp-ID: ALA / End label comp-ID: ALA / Refine code: _ / Auth seq-ID: 89 - 425 / Label seq-ID: 1 - 337
Dom-ID
Ens-ID
Auth asym-ID
Label asym-ID
1
1
A
A
2
1
B
B
1
2
A
A
2
2
C
C
1
3
A
A
2
3
D
D
1
4
B
B
2
4
C
C
1
5
B
B
2
5
D
D
1
6
C
C
2
6
D
D
NCS ensembles :
ID
1
2
3
4
5
6
-
Components
#1: Protein
fructose-1,6-bisphosphatase
Mass: 36660.176 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Physcomitrella patens subsp. patens (plant) Gene: PHYPADRAFT_233069 / Production host: Physcomitrella patens (plant) / References: UniProt: A9T230
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.29 Å3/Da / Density % sol: 46.35 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5 / Details: 25 % PEG 3350 0.1 M Bis-Tris
Resolution: 3→102.34 Å / Cor.coef. Fo:Fc: 0.861 / Cor.coef. Fo:Fc free: 0.799 / SU B: 33.964 / SU ML: 0.637 / Cross valid method: THROUGHOUT / ESU R Free: 0.796 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.31508
931
5 %
RANDOM
Rwork
0.25409
-
-
-
obs
0.25711
17748
72.95 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å