Component-ID: _ / Ens-ID: 1 / Beg auth comp-ID: LYS / Beg label comp-ID: LYS / End auth comp-ID: SER / End label comp-ID: SER / Refine code: _ / Auth seq-ID: 8 - 481 / Label seq-ID: 8 - 481
Dom-ID
Auth asym-ID
Label asym-ID
1
A
A
2
B
B
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Components
#1: Protein
SLIT-ROBORhoGTPase-activatingprotein2 / srGAP2 / Formin-binding protein 2 / Rho GTPase-activating protein 34
Mass: 56555.230 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SRGAP2, ARHGAP34, FNBP2, KIAA0456, SRGAP2A / Production host: Homo sapiens (human) / References: UniProt: O75044
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.05 Å3/Da / Density % sol: 59.65 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop Details: F-BARx eluted in 5% Glycerol, 25 mM Tris buffer pH=8.5, 1 mM DTT, and ~0.2 M NaCl at the elution peak, concentrated to ~15 mg/ml and crystallized in 0.2 M Ammonium citrate tribasic pH 6.5, 0. ...Details: F-BARx eluted in 5% Glycerol, 25 mM Tris buffer pH=8.5, 1 mM DTT, and ~0.2 M NaCl at the elution peak, concentrated to ~15 mg/ml and crystallized in 0.2 M Ammonium citrate tribasic pH 6.5, 0.2 M Guanidine, 0.3 M NaCl, 16% PEG 3350