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- PDB-5hvc: Solution structure of the apo state of the acyl carrier protein f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5hvc | ||||||
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Title | Solution structure of the apo state of the acyl carrier protein from the MLSA2 subunit of the mycolactone polyketide synthase | ||||||
![]() | Type I modular polyketide synthase | ||||||
![]() | TRANSFERASE / acyl carrier protein mycolactone | ||||||
Function / homology | ![]() phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / oxidoreductase activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Vance, S. / Tkachenko, O. / Thomas, B. / Bassuni, M. / Hong, H. / Nietlispach, D. / Broadhurst, R.W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase. Authors: Vance, S. / Tkachenko, O. / Thomas, B. / Bassuni, M. / Hong, H. / Nietlispach, D. / Broadhurst, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 609.2 KB | Display | ![]() |
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PDB format | ![]() | 519.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 469.8 KB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 80 KB | Display | |
Data in CIF | ![]() | 101.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5hv8C C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10237.473 Da / Num. of mol.: 1 Fragment: Acyl carrier protein fragment, UNP residues 2050 to 2140 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 150 mM sodium phosphate, 0.1 mM 333-trimethylsilylpropionate, sodium salt, 90% H2O/10% D2O Details: The sample was prepared at a concentration of 800 micromolar in phosphate buffer supplemented with 10 % D2O (Sigma) and 0.0025 % 3,3,3-trimethylsilylpropionate (Sigma) in 5 mm Ultra-Imperial ...Details: The sample was prepared at a concentration of 800 micromolar in phosphate buffer supplemented with 10 % D2O (Sigma) and 0.0025 % 3,3,3-trimethylsilylpropionate (Sigma) in 5 mm Ultra-Imperial grade NMR tubes (Wilmad) to a final volume of 600 microlitres. Label: 15N13C_sample / Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 150 mM / Ionic strength err: 25 / Label: conditions_1 / pH: 7.5 / PH err: 0.05 / Pressure: 1 atm / Temperature: 283 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 3 Details: The structures are based on 2839 distance restraints, no hydrogen bond restraints, and 167 backbone dihedral angle restraints defined using the DANGLE algorithm in CCPN Analysis | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |