温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Crystallography was performed using the sitting drop method using 20 mg/ml CYP144A1. Drops were prepared by the addition of 0.2 microl of CYP144A1-FLV and -TRV proteins to 0.2 microl of ...詳細: Crystallography was performed using the sitting drop method using 20 mg/ml CYP144A1. Drops were prepared by the addition of 0.2 microl of CYP144A1-FLV and -TRV proteins to 0.2 microl of mother liquor and by incubating at 4 oC. Following initial crystallogenesis using commercial screens, crystallization conditions were further refined to 0.8 or 1.0 M (NH4)2SO4 with 0.1 M HEPES, pH 7.55, and 25% PEG 3350. Single crystals were flash cooled after addition of 10% PEG 200 as cryoprotectant
解像度: 1.54→84.77 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.961 / SU B: 3.32 / SU ML: 0.053 / 交差検証法: THROUGHOUT / ESU R: 0.076 / ESU R Free: 0.073 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.19238
6118
5 %
RANDOM
Rwork
0.13761
-
-
-
obs
0.14028
116903
98.45 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK