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Yorodumi- PDB-5gin: Crystal structure of box C/D RNP with 12 nt guide regions and 9 n... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5gin | ||||||
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Title | Crystal structure of box C/D RNP with 12 nt guide regions and 9 nt substrates | ||||||
Components |
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Keywords | TRANSFERASE/RNA / 2'-O-methylation / guide RNA / RNP / TRANSFERASE-RNA complex | ||||||
Function / homology | Function and homology information histone H2AQ104 methyltransferase activity / box C/D sno(s)RNA 3'-end processing / rRNA methyltransferase activity / box C/D methylation guide snoRNP complex / ribonuclease P activity / tRNA 5'-leader removal / rRNA methylation / tRNA processing / snoRNA binding / Transferases; Transferring one-carbon groups; Methyltransferases ...histone H2AQ104 methyltransferase activity / box C/D sno(s)RNA 3'-end processing / rRNA methyltransferase activity / box C/D methylation guide snoRNP complex / ribonuclease P activity / tRNA 5'-leader removal / rRNA methylation / tRNA processing / snoRNA binding / Transferases; Transferring one-carbon groups; Methyltransferases / small-subunit processome / methyltransferase activity / rRNA processing / ribosome biogenesis / methylation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / RNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.308 Å | ||||||
Authors | Yang, Z. / Lin, J. / Ye, K. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2016 Title: Box C/D guide RNAs recognize a maximum of 10 nt of substrates Authors: Yang, Z. / Lin, J. / Ye, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gin.cif.gz | 505.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gin.ent.gz | 406.2 KB | Display | PDB format |
PDBx/mmJSON format | 5gin.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gi/5gin ftp://data.pdbj.org/pub/pdb/validation_reports/gi/5gin | HTTPS FTP |
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-Related structure data
Related structure data | 5gioC 5gipC 3plaS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
-Protein , 3 types, 9 molecules ABKCDLEFM
#1: Protein | Mass: 44168.531 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Gene: SULA_1947, SULB_1948, SULC_1946 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E3MJI1, UniProt: Q97ZH3*PLUS #2: Protein | Mass: 14075.301 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Gene: rpl7ae, SULA_1106, SULB_1107, SULC_1105 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E3JZF7, UniProt: P55858*PLUS #3: Protein | Mass: 26439.375 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Gene: flpA, SSOP1_0970, SULA_1948, SULB_1949, SULC_1947 / Production host: Escherichia coli (E. coli) References: UniProt: A0A0E3JUC9, UniProt: P58032*PLUS, Transferases; Transferring one-carbon groups; Methyltransferases |
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-RNA chain , 2 types, 6 molecules GHNIJO
#4: RNA chain | Mass: 12852.657 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Sulfolobus solfataricus (archaea) #5: RNA chain | Mass: 2871.767 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Sulfolobus solfataricus (archaea) |
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-Non-polymers , 1 types, 3 molecules
#6: Chemical |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.58 Å3/Da / Density % sol: 65.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 2.0 M ammonium sulfate, 2% v/v PEG 400, 10 mM magnesium chloride, 0.1 M HEPES |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9789 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 28, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→20 Å / Num. obs: 63174 / % possible obs: 96.5 % / Redundancy: 7.2 % / Rmerge(I) obs: 0.131 / Net I/σ(I): 12 |
Reflection shell | Resolution: 3.3→3.36 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3PLA Resolution: 3.308→19.999 Å / SU ML: 0.53 / Cross valid method: FREE R-VALUE / σ(F): 0.11 / Phase error: 32.82
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.308→19.999 Å
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Refine LS restraints |
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LS refinement shell |
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