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- PDB-5fgj: Structure of tetrameric rat phenylalanine hydroxylase, residues 1-453 -
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Open data
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Basic information
Entry | Database: PDB / ID: 5fgj | ||||||
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Title | Structure of tetrameric rat phenylalanine hydroxylase, residues 1-453 | ||||||
![]() | Phenylalanine-4-hydroxylase | ||||||
![]() | OXIDOREDUCTASE / phenylketonuria / PKU mutation / allostery / ACT domain | ||||||
Function / homology | ![]() tyrosine biosynthetic process, by oxidation of phenylalanine / Phenylalanine metabolism / L-phenylalanine metabolic process / phenylalanine 4-monooxygenase / phenylalanine 4-monooxygenase activity / tyrosine biosynthetic process / L-phenylalanine catabolic process / amino acid binding / iron ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Taylor, A.B. / Roberts, K.M. / Fitzpatrick, P.F. | ||||||
![]() | ![]() Title: Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-ray Scattering. Authors: Meisburger, S.P. / Taylor, A.B. / Khan, C.A. / Zhang, S. / Fitzpatrick, P.F. / Ando, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 302.4 KB | Display | ![]() |
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PDB format | ![]() | 249.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457.2 KB | Display | ![]() |
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Full document | ![]() | 470.7 KB | Display | |
Data in XML | ![]() | 56.1 KB | Display | |
Data in CIF | ![]() | 75.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5egqC ![]() 2phmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51885.582 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-FE / #3: Chemical | ChemComp-MG / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.37 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 25% PEG 3350, 0.2 M magnesium chloride, 0.1 M bis-tris:HCl |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 7, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 3.6→102.69 Å / Num. obs: 24188 / % possible obs: 100 % / Redundancy: 8.2 % / Biso Wilson estimate: 75.6 Å2 / Rsym value: 0.388 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 3.6→3.79 Å / Redundancy: 8.3 % / Rmerge(I) obs: 1.287 / Mean I/σ(I) obs: 2.2 / Num. unique all: 1688 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2PHM Resolution: 3.6→101.48 Å / SU ML: 0.5 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 29.64 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.6→101.48 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.6→3.69 Å
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