- PDB-5f0q: Crystal structure of C-terminal domain of the human DNA primase l... -
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基本情報
登録情報
データベース: PDB / ID: 5f0q
タイトル
Crystal structure of C-terminal domain of the human DNA primase large subunit with bound DNA template/RNA primer
要素
DNA (5'-D(*GP*CP*CP*GP*CP*CP*AP*AP*CP*AP*TP*A)-3')
DNA primase large subunit
RNA (5'-R(P*GP*GP*CP*GP*GP*C)-3')
キーワード
Tranferase/DNA/RNA / Tranferase-DNA-RNA complex / DNA primase / large subunit / iron-sulfur cluster / RNA / DNA / primer / template / triphosphate / initiation site
機能・相同性
機能・相同性情報
positive regulation of DNA primase activity / DNA replication initiation / DNA/RNA hybrid binding / Telomere C-strand synthesis initiation / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / alpha DNA polymerase:primase complex / Polymerase switching / Processive synthesis on the lagging strand / Removal of the Flap Intermediate / Polymerase switching on the C-strand of the telomere ...positive regulation of DNA primase activity / DNA replication initiation / DNA/RNA hybrid binding / Telomere C-strand synthesis initiation / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / alpha DNA polymerase:primase complex / Polymerase switching / Processive synthesis on the lagging strand / Removal of the Flap Intermediate / Polymerase switching on the C-strand of the telomere / primosome complex / DNA replication, synthesis of primer / Activation of the pre-replicative complex / DNA replication initiation / Defective pyroptosis / 4 iron, 4 sulfur cluster binding / DNA binding / nucleoplasm / metal ion binding 類似検索 - 分子機能
DNA primase, large subunit, eukaryotic / DNA primase large subunit, eukaryotic/archaeal / Eukaryotic and archaeal DNA primase, large subunit 類似検索 - ドメイン・相同性
IRON/SULFUR CLUSTER / DNA / DNA (> 10) / RNA / DNA primase large subunit 類似検索 - 構成要素
#236 - 2019年8月 サイクリンとサイクリン依存性キナーゼ (Cyclin and Cyclin-dependent Kinase) 類似性 (1)
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集合体
登録構造単位
A: DNA primase large subunit B: DNA primase large subunit C: RNA (5'-R(P*GP*GP*CP*GP*GP*C)-3') D: DNA (5'-D(*GP*CP*CP*GP*CP*CP*AP*AP*CP*AP*TP*A)-3') E: RNA (5'-R(P*GP*GP*CP*GP*GP*C)-3') F: DNA (5'-D(*GP*CP*CP*GP*CP*CP*AP*AP*CP*AP*TP*A)-3') ヘテロ分子
マシュー密度: 2.94 Å3/Da / 溶媒含有率: 58.1 % / 解説: needle-like prisms with a square in cross-section
結晶化
温度: 295 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: 0.18 M lithium sulfate, 50 mM sodium citrate, 2 mM TCEP, 0.1 M sodium malonate, 0.1 M Hepes-NaOH (pH 7.0), 19.7% PEG 8,000 and 0.02 M magnesium chloride