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- PDB-5f0p: Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1... -

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Basic information

Entry
Database: PDB / ID: 5f0p
TitleStructure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1(L557M) (SeMet labeled)
Components
  • (Vacuolar protein sorting-associated protein ...Vacuole) x 2
  • Natural resistance-associated macrophage protein 2
  • Sorting nexin-3
KeywordsPROTEIN TRANSPORT / retromer / sorting nexin
Function / homology
Function and homology information


vanadium ion transmembrane transporter activity / vanadium ion transport / transition metal ion transmembrane transporter activity / paraferritin complex / Defective SLC11A2 causes hypochromic microcytic anemia, with iron overload 1 (AHMIO1) / lead ion transmembrane transporter activity / nickel cation transmembrane transporter activity / lead ion transport / negative regulation of early endosome to late endosome transport / cadmium ion transmembrane transport ...vanadium ion transmembrane transporter activity / vanadium ion transport / transition metal ion transmembrane transporter activity / paraferritin complex / Defective SLC11A2 causes hypochromic microcytic anemia, with iron overload 1 (AHMIO1) / lead ion transmembrane transporter activity / nickel cation transmembrane transporter activity / lead ion transport / negative regulation of early endosome to late endosome transport / cadmium ion transmembrane transport / late endosome to Golgi transport / protein to membrane docking / nickel cation transport / negative regulation of protein transport / positive regulation of locomotion involved in locomotory behavior / neurotransmitter receptor transport, endosome to plasma membrane / solute:proton symporter activity / negative regulation of protein localization / regulation of postsynapse assembly / mitochondrion-derived vesicle / inorganic cation transmembrane transporter activity / Metal ion SLC transporters / negative regulation of protein homooligomerization / manganese ion transport / membrane invagination / iron ion transmembrane transport / regulation of dendritic spine maintenance / manganese ion transmembrane transporter activity / zinc ion transmembrane transporter activity / tubular endosome / mitochondrion to lysosome vesicle-mediated transport / detection of oxygen / positive regulation of Wnt protein secretion / iron ion transmembrane transporter activity / cadmium ion transmembrane transporter activity / regulation of terminal button organization / cobalt ion transport / retromer, cargo-selective complex / cobalt ion transmembrane transporter activity / vesicle-mediated transport in synapse / WNT ligand biogenesis and trafficking / intralumenal vesicle formation / iron import into cell / retromer complex binding / negative regulation of late endosome to lysosome transport / copper ion transmembrane transporter activity / negative regulation of lysosomal protein catabolic process / positive regulation of dopamine receptor signaling pathway / positive regulation of dopamine biosynthetic process / ferrous iron transmembrane transporter activity / phosphatidylinositol-5-phosphate binding / dopaminergic synapse / mitochondrial fragmentation involved in apoptotic process / regulation of protein metabolic process / retromer complex / neurotransmitter receptor transport, endosome to postsynaptic membrane / protein localization to endosome / regulation of synapse maturation / voluntary musculoskeletal movement / copper ion transport / negative regulation of viral entry into host cell / basal part of cell / phosphatidylinositol-3-phosphate binding / transcytosis / endocytic recycling / early phagosome / positive regulation of protein localization to cell periphery / vacuole / phosphatidylinositol-4-phosphate binding / negative regulation of phagocytosis / retrograde transport, endosome to Golgi / phosphatidylinositol-3,5-bisphosphate binding / response to iron ion / regulation of mitochondrion organization / regulation of Wnt signaling pathway / dendrite morphogenesis / clathrin-coated vesicle / heme biosynthetic process / erythrocyte development / positive regulation of mitochondrial fission / lysosome organization / cadmium ion binding / regulation of presynapse assembly / D1 dopamine receptor binding / regulation of macroautophagy / brush border membrane / response to bacterium / Iron uptake and transport / intracellular protein transport / modulation of chemical synaptic transmission / protein destabilization / regulation of protein stability / trans-Golgi network / negative regulation of protein catabolic process / positive regulation of neuron projection development / recycling endosome / negative regulation of inflammatory response / Wnt signaling pathway / multicellular organismal-level iron ion homeostasis / recycling endosome membrane
Similarity search - Function
Vertebrate SNX3, PX domain / Vacuolar protein sorting-associated protein 35 / Vacuolar protein sorting protein 26 related / Vacuolar protein sorting-associated protein 35, C-terminal / Vacuolar protein sorting-associated protein 35 / Vacuolar protein sorting-associated protein 26 / Immunoglobulin-like - #640 / NRAMP family / Natural resistance-associated macrophage protein-like / Phox-like domain ...Vertebrate SNX3, PX domain / Vacuolar protein sorting-associated protein 35 / Vacuolar protein sorting protein 26 related / Vacuolar protein sorting-associated protein 35, C-terminal / Vacuolar protein sorting-associated protein 35 / Vacuolar protein sorting-associated protein 26 / Immunoglobulin-like - #640 / NRAMP family / Natural resistance-associated macrophage protein-like / Phox-like domain / PX Domain / Arrestin-like, C-terminal / PhoX homologous domain, present in p47phox and p40phox. / PX domain profile. / PX domain / Phox homology / PX domain superfamily / Armadillo-type fold / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Sorting nexin-3 / Vacuolar protein sorting-associated protein 26A / Natural resistance-associated macrophage protein 2 / Vacuolar protein sorting-associated protein 35
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.78 Å
AuthorsLucas, M. / Gershlick, D. / Vidaurrazaga, A. / Rojas, A.L. / Bonifacino, J.S. / Hierro, A.
Funding support Spain, 3items
OrganizationGrant numberCountry
Carlos III Health InstitutePI11/00121 Spain
Basque GovernmentPI2011-26 Spain
Spanish Ministry of Economy and CompetitivenessBFU2014-59759-R Spain
CitationJournal: Cell / Year: 2016
Title: Structural Mechanism for Cargo Recognition by the Retromer Complex.
Authors: Lucas, M. / Gershlick, D.C. / Vidaurrazaga, A. / Rojas, A.L. / Bonifacino, J.S. / Hierro, A.
History
DepositionNov 27, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 7, 2016Provider: repository / Type: Initial release
Revision 1.1Dec 14, 2016Group: Database references
Revision 1.2Nov 29, 2017Group: Database references / Category: pdbx_database_related
Revision 1.3Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / diffrn_radiation_wavelength / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Vacuolar protein sorting-associated protein 35
B: Vacuolar protein sorting-associated protein 26A
C: Sorting nexin-3
D: Natural resistance-associated macrophage protein 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)114,93035
Polymers112,6794
Non-polymers2,25231
Water75742
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12870 Å2
ΔGint-88 kcal/mol
Surface area45650 Å2
MethodPISA
Unit cell
Length a, b, c (Å)371.290, 75.320, 57.270
Angle α, β, γ (deg.)90.000, 97.660, 90.000
Int Tables number5
Space group name H-MC121

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Components

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Vacuolar protein sorting-associated protein ... , 2 types, 2 molecules AB

#1: Protein Vacuolar protein sorting-associated protein 35 / Vacuole / hVPS35 / Maternal-embryonic 3 / Vesicle protein sorting 35


Mass: 53288.270 Da / Num. of mol.: 1 / Fragment: Residues 14-470
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: VPS35, MEM3, TCCCTA00141 / Plasmid: pGST-Parallel2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q96QK1
#2: Protein Vacuolar protein sorting-associated protein 26A / Vacuole / Vesicle protein sorting 26A / hVPS26


Mass: 37913.312 Da / Num. of mol.: 1 / Fragment: Residues 1-321
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: VPS26A, VPS26 / Plasmid: pET-Sumo3 / Production host: Escherichia coli (E. coli) / Variant (production host): B834(DE3) / References: UniProt: O75436

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Protein / Protein/peptide , 2 types, 2 molecules CD

#3: Protein Sorting nexin-3 / / Protein SDP3


Mass: 19193.814 Da / Num. of mol.: 1 / Fragment: Residues 1-162
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SNX3 / Plasmid: pHis-MBP-Parallel2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O60493
#4: Protein/peptide Natural resistance-associated macrophage protein 2 / NRAMP 2 / Divalent cation transporter 1 / Divalent metal transporter 1 / DMT-1 / Solute carrier ...NRAMP 2 / Divalent cation transporter 1 / Divalent metal transporter 1 / DMT-1 / Solute carrier family 11 member 2 / Divalent cation transporter II


Mass: 2283.244 Da / Num. of mol.: 1 / Mutation: L557M
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SLC11A2, DCT1, DMT1, NRAMP2, OK/SW-cl.20 / Plasmid: pET-Sumo3 / Production host: Escherichia coli (E. coli) / Variant (production host): B834(DE3) / References: UniProt: P49281

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Non-polymers , 5 types, 73 molecules

#5: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: SO4
#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 20 / Source method: obtained synthetically / Formula: C2H6O2
#8: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
#9: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 42 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.52 Å3/Da / Density % sol: 65.11 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 0.8 M AmSO4, 0.1 M MES pH 6.0, 10% Glycerol

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.97895 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 23, 2015
RadiationMonochromator: Si(111) double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97895 Å / Relative weight: 1
ReflectionResolution: 2.77→56.761 Å / Num. obs: 77507 / % possible obs: 99.1 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 80.168 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.066 / Rrim(I) all: 0.078 / Χ2: 1.043 / Net I/σ(I): 12.17 / Num. measured all: 271917
Reflection shell

Diffraction-ID: 1 / Rejects: 0

Resolution (Å)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
2.77-2.940.9041.334090612604120130.8081.07395.3
2.94-3.140.5212.314158011906119010.9190.617100
3.14-3.390.2524.63981411099110740.980.29799.8
3.39-3.710.1238.513464410090100850.9930.146100
3.71-4.150.07414.1633137923792290.9960.08799.9
4.15-4.790.04821.5228280810180910.9970.05799.9
4.79-5.850.04225.2724788687568660.9980.0599.9
5.85-8.230.03628.7218466532453070.9980.04299.7
8.23-56.7610.02444.8710302296729410.9990.02999.1

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Processing

Software
NameVersionClassification
REFMAC5.8.0155refinement
XSCALEdata scaling
PDB_EXTRACT3.15data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5F0L

5f0l


Resolution: 2.78→56.76 Å / Cor.coef. Fo:Fc: 0.928 / Cor.coef. Fo:Fc free: 0.894 / WRfactor Rfree: 0.2344 / WRfactor Rwork: 0.1928 / FOM work R set: 0.7671 / SU B: 16.993 / SU ML: 0.294 / SU R Cruickshank DPI: 0.7397 / SU Rfree: 0.3443 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.74 / ESU R Free: 0.344 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
RfactorNum. reflection% reflectionSelection details
Rfree0.2605 1901 5 %RANDOM
Rwork0.2189 ---
obs0.2209 36032 95.13 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 171.69 Å2 / Biso mean: 70.57 Å2 / Biso min: 17.41 Å2
Baniso -1Baniso -2Baniso -3
1--0.04 Å20 Å2-0.73 Å2
2--0.09 Å20 Å2
3---0.14 Å2
Refinement stepCycle: final / Resolution: 2.78→56.76 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7453 0 135 42 7630
Biso mean--80.77 37.38 -
Num. residues----913
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0070.0197702
X-RAY DIFFRACTIONr_bond_other_d0.0010.027535
X-RAY DIFFRACTIONr_angle_refined_deg1.1351.98710359
X-RAY DIFFRACTIONr_angle_other_deg0.851317323
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.795909
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.63224.271377
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.526151396
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.6511555
X-RAY DIFFRACTIONr_chiral_restr0.0640.21166
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.0218473
X-RAY DIFFRACTIONr_gen_planes_other0.0010.021731
X-RAY DIFFRACTIONr_mcbond_it2.5127.0653648
X-RAY DIFFRACTIONr_mcbond_other2.5127.0653647
X-RAY DIFFRACTIONr_mcangle_it4.41410.5874553
LS refinement shellResolution: 2.777→2.849 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.443 69 -
Rwork0.433 1347 -
all-1416 -
obs--48.36 %

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