+Open data
-Basic information
Entry | Database: PDB / ID: 5c6g | ||||||
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Title | Structural Insights into the Scc2-Scc4 Cohesin Loader | ||||||
Components |
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Keywords | CELL CYCLE / cohesin loader / TPR repeat | ||||||
Function / homology | Function and homology information positive regulation of cohesin loading / SMC loading complex / Scc2-Scc4 cohesin loading complex / mitotic cohesin loading / 2-micrometer circle DNA / tRNA gene clustering / rDNA chromatin condensation / establishment of protein localization to chromatin / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / kinetochore binding ...positive regulation of cohesin loading / SMC loading complex / Scc2-Scc4 cohesin loading complex / mitotic cohesin loading / 2-micrometer circle DNA / tRNA gene clustering / rDNA chromatin condensation / establishment of protein localization to chromatin / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / kinetochore binding / chromatin => GO:0000785 / replication-born double-strand break repair via sister chromatid exchange / establishment of mitotic sister chromatid cohesion / mitotic chromosome condensation / mitotic sister chromatid cohesion / double-strand break repair / regulation of gene expression / sequence-specific DNA binding / chromatin binding / chromatin / cytosol Similarity search - Function | ||||||
Biological species | Ashbya gossypii (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.6 Å | ||||||
Authors | Singleton, M.R. / Chao, W.C.H. | ||||||
Citation | Journal: Cell Rep / Year: 2015 Title: Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader. Authors: Chao, W.C. / Murayama, Y. / Munoz, S. / Costa, A. / Uhlmann, F. / Singleton, M.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5c6g.cif.gz | 292 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5c6g.ent.gz | 238.9 KB | Display | PDB format |
PDBx/mmJSON format | 5c6g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c6/5c6g ftp://data.pdbj.org/pub/pdb/validation_reports/c6/5c6g | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 70537.055 Da / Num. of mol.: 2 / Fragment: RESIDUES 34-653 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ashbya gossypii (fungus) / Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056 / Gene: AGOS_AGR133C / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q74ZR5 #2: Protein | Mass: 24624.908 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (fungus) Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056 / Gene: SCC2, AGL133W / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q750S2 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.93 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: PEG 6000, cacodylate. calcium acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 21, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2.55→98.66 Å / Num. obs: 63440 / % possible obs: 96.76 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 17.96 |
Reflection shell | Resolution: 2.55→2.62 Å / Rmerge(I) obs: 0.591 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.6→98.648 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 31.71 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→98.648 Å
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Refine LS restraints |
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LS refinement shell |
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