[English] 日本語
![](img/lk-miru.gif)
- PDB-5anm: Crystal structure of IgE Fc in complex with a neutralizing antibody -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 5anm | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of IgE Fc in complex with a neutralizing antibody | |||||||||
![]() |
| |||||||||
![]() | IMMUNE SYSTEM / THERAPEUTIC ANTIBODY / IGG / IGE / ASTHMA | |||||||||
Function / homology | ![]() adaptive immune memory response / primary adaptive immune response / IgE B cell receptor complex / type I hypersensitivity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / eosinophil degranulation / IgE immunoglobulin complex / macrophage activation / type 2 immune response ...adaptive immune memory response / primary adaptive immune response / IgE B cell receptor complex / type I hypersensitivity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / eosinophil degranulation / IgE immunoglobulin complex / macrophage activation / type 2 immune response / Fc epsilon receptor (FCERI) signaling / antibody-dependent cellular cytotoxicity / mast cell degranulation / B cell proliferation / macrophage differentiation / Role of LAT2/NTAL/LAB on calcium mobilization / immunoglobulin complex, circulating / immunoglobulin receptor binding / FCERI mediated Ca+2 mobilization / complement activation, classical pathway / FCERI mediated MAPK activation / antigen binding / B cell receptor signaling pathway / FCERI mediated NF-kB activation / antibacterial humoral response / Interleukin-4 and Interleukin-13 signaling / adaptive immune response / blood microparticle / inflammatory response / immune response / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Cohen, E.S. / Dobson, C.L. / Kack, H. / Wang, B. / Sims, D.A. / Lloyd, C.O. / England, E. / Rees, D.G. / Guo, H. / Karagiannis, S.N. ...Cohen, E.S. / Dobson, C.L. / Kack, H. / Wang, B. / Sims, D.A. / Lloyd, C.O. / England, E. / Rees, D.G. / Guo, H. / Karagiannis, S.N. / O'Brien, S. / Persdotter, S. / Ekdahl, H. / Butler, R. / Keyes, F. / Oakley, S. / Carlsson, M. / Briend, E. / Wilkinson, T. / Anderson, I.K. / Monk, P.D. / vonWachenfeldt, K. / Eriksson, P.O. / Gould, H.J. / Vaughan, T.J. / May, R.D. | |||||||||
![]() | ![]() Title: A Novel Ige-Neutralizing Antibody for the Treatment of Severe Uncontrolled Asthma. Authors: Cohen, E.S. / Dobson, C.L. / Kack, H. / Wang, B. / Sims, D.A. / Lloyd, C.O. / England, E. / Rees, D.G. / Guo, H. / Karagiannis, S.N. / O'Brien, S. / Persdotter, S. / Ekdahl, H. / Butler, R. ...Authors: Cohen, E.S. / Dobson, C.L. / Kack, H. / Wang, B. / Sims, D.A. / Lloyd, C.O. / England, E. / Rees, D.G. / Guo, H. / Karagiannis, S.N. / O'Brien, S. / Persdotter, S. / Ekdahl, H. / Butler, R. / Keyes, F. / Oakley, S. / Carlsson, M. / Briend, E. / Wilkinson, T. / Anderson, I.K. / Monk, P.D. / Von Wachenfeldt, K. / Eriksson, P.F. / Gould, H.J. / Vaughan, T.J. / May, R.D. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 746.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 624.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 71.1 KB | Display | |
Data in CIF | ![]() | 97.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
-Antibody , 2 types, 6 molecules ACLBDH
#1: Antibody | Mass: 22930.264 Da / Num. of mol.: 3 / Fragment: FAB, LIGHT CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 24752.887 Da / Num. of mol.: 3 / Fragment: FAB, HEAVY CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|
-Protein / Non-polymers , 2 types, 193 molecules EFG![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#3: Protein | Mass: 27341.619 Da / Num. of mol.: 3 / Fragment: FC DOMAIN, CE3-CE4, RESIDUES 211-428 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #7: Water | ChemComp-HOH / | |
---|
-Sugars , 3 types, 3 molecules
#4: Polysaccharide | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
---|---|
#5: Polysaccharide | beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#6: Polysaccharide | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[beta-D-mannopyranose-(1-3)]beta-D- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[beta-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
-Details
Sequence details | THIS IS A UNIQUE FAB RAISED AGAINST IGE |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59 % / Description: NONE |
---|---|
Crystal grow | Details: 100 MM MGCL2, 100 MM SODIUM CITRATE PH 5.0 AND 15% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 19, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→109.11 Å / Num. obs: 66338 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 10.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 2.85→2.92 Å / Redundancy: 11 % / Rmerge(I) obs: 0.75 / Mean I/σ(I) obs: 3.4 / % possible all: 100 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 1AQK, 1FP5 Resolution: 2.85→31.29 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.887 / SU B: 30.044 / SU ML: 0.281 / Cross valid method: THROUGHOUT / ESU R: 1.133 / ESU R Free: 0.366 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 68.613 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.85→31.29 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|