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- PDB-4wjw: Crystal Structure of the Chs5-Chs6 Exomer Cargo Adaptor Complex B... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4wjw | |||||||||
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Title | Crystal Structure of the Chs5-Chs6 Exomer Cargo Adaptor Complex Bound to portion of Chs3 | |||||||||
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![]() | transport protein/transferase / Adaptor protein / cargo binding / vesicular trafficking / chitin synthase / Biosynthetic Protein / transport protein-transferase complex | |||||||||
Function / homology | ![]() : / exomer complex / protein localization to bud neck / : / conjugation with cellular fusion / chitosome / chitin synthase / chitin synthase activity / prospore membrane / cellular bud site selection ...: / exomer complex / protein localization to bud neck / : / conjugation with cellular fusion / chitosome / chitin synthase / chitin synthase activity / prospore membrane / cellular bud site selection / ascospore wall assembly / incipient cellular bud site / Golgi to plasma membrane transport / cellular bud neck / cell septum / mating projection tip / cell periphery / trans-Golgi network / cytoplasmic vesicle membrane / small GTPase binding / protein transport / membrane => GO:0016020 / protein dimerization activity / regulation of DNA-templated transcription / endoplasmic reticulum / membrane / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Weiskoff, A.M. / Fromme, J.C. | |||||||||
![]() | ![]() Title: Distinct N-terminal regions of the exomer secretory vesicle cargo Chs3 regulate its trafficking itinerary. Authors: Weiskoff, A.M. / Fromme, J.C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 318.9 KB | Display | ![]() |
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PDB format | ![]() | 259 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4gns S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 8653.009 Da / Num. of mol.: 1 / Fragment: unp residues 1-77 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CHS5, CAL3, YLR330W, L8543.18 / Production host: ![]() ![]() |
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#2: Protein | Mass: 87977.008 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CHS6, CSD3, YJL099W, J0838 / Production host: ![]() ![]() |
#3: Protein/peptide | Mass: 2348.505 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.8 Å3/Da / Density % sol: 74.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.5 / Details: 0.3 M AMMONIUM SULFATE, 0.1 M CITRIC ACID / PH range: 4.0-4.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Mar 16, 2012 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9767 Å / Relative weight: 1 |
Reflection | Resolution: 2.59→44.65 Å / Num. obs: 59936 / % possible obs: 99 % / Redundancy: 12.2 % / Rsym value: 0.09 / Net I/σ(I): 19.45 |
Reflection shell | Resolution: 2.59→2.683 Å / % possible all: 99 |
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Processing
Software | Name: PHENIX / Version: (PHENIX.REFINE: 1.8.4_1496) / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: ![]() Starting model: 4GNS ![]() 4gns Resolution: 2.59→44.65 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 20.44 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.59→44.65 Å
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LS refinement shell |
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